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The tumor suppressor Adenomatous Polyposis Coli (APC) gene is associated with susceptibility to schizophrenia

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The tumor suppressor Adenomatous Polyposis Coli (APC) gene i...

自体骨髓单个核细胞经介入途径移植治疗股骨头坏死54例:12个月疗效随访

第六次全国医学遗传学学术会议

作者： DH Cui, KD Jiang, SD Jiang, yf xu, H Yao Department of Psychiatry, Fudan University Affiliated Huashan Hospital, Shanghai, 200030, PR China Shanghai Mental Health Center, Shanghai, 200030, PR China Xinjiang Medical University Affiliated Traditional Chinese Medicine Hospital, Urumqi, 830000, PR China

The etiology of schizophrenia is unclear, although family, twin and linkage studies implicate genetic factors. Here we identified APC, a tumor suppressor gene, as a risk factor for schizophrenia. We compared leucocytic gene expression patterns of six pairs of patients with schizophrenia and healthy controls by microarray. APC expression levels were significantly increased in all patients compared to healthy controls. To confirm the finding of microarray analysis, we measured expression levels of APC in the leucocytes from 30 relapse patients taking antipsychotic, 29 first episodic drug-naive patients, and 30 healthy controls using real-time quantitative RT-PCR. APC expression levels were significantly increased in leucocytes of schizophrenics both taking and not taking antipsychotic, hence the increase of APC expression was not due to antipsychotic medication. APC is located at 5q21-22, which has been previously reported to be linked with schizophrenia. Further, we performed the transmission disequilibrium test (TDT) and TDT based on haplotypes to search for the association between schizophrenia and APC by examined 163 parent-offspring trios of Chinese descent. We analyzed 3 single nucleotide polymorphisms (SNPs) (rs2229992, rs42427, rs465899) at the exon region of APC. The TDT showed that the 3 SNPs are significantly associated with schizophrenia (TDTx2=4.23, P<0.05;4.15, P<0.05;8.49 P<0.01 respectively;HHRRx2 =5.54, P<0.05;4.40, P<0.05;9.79, P<0.01 respectively). We found a significant association between the APC haplotypes from rs2229992-rs42427-rs465899 and schizophrenia (Global x2 = 44.376,df=7, .P<0.001). The C-A-T haplotype has a frequency of more than 57% and has a strong association with schizophrenia (x2 =15.04, P<0.001). These results indicate that the APC may be a candidate gene conferring susceptibility to schizophrenia and also may be associated with reduced vulnerability to cancer in schizophrenia.

关键词： schizophrenia APC gene expression TDT haplotypes

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中国组织工程研究与临床康复 2007年第15期11卷 2904-2906页

作者：许忆峰杨晓凤王红梅张轶斌吴雁翔吕乃武单鸿吕欣解放军第四六三医院细胞治疗中心辽宁省沈阳市110042

目的:观察经介入途径移植自体骨髓单个核细胞在股骨头坏死治疗中的应用,并评价其疗效。方法:选择2004-07/2005-11在解放军四六三院细胞治疗中心住院的,具有完整随访资料的股骨头坏死确诊患者共54例91髋。纳入确诊股骨头坏死,有关节疼痛... 详细信息

目的:观察经介入途径移植自体骨髓单个核细胞在股骨头坏死治疗中的应用,并评价其疗效。方法:选择2004-07/2005-11在解放军四六三院细胞治疗中心住院的,具有完整随访资料的股骨头坏死确诊患者共54例91髋。纳入确诊股骨头坏死,有关节疼痛、功能障碍等症状患者,性别、年龄不限;排除有严重心力衰竭、严重肾功能异常等不能耐受手术者。符合纳入标准54例,男45例,女9例,12～68岁。按ARCO分期Ⅱ期42髋,Ⅲ期47髋,Ⅳ期2髋。实验对象对治疗的相关内容知情同意并签知情同意。干预措施:抽取患者髂后上嵴骨髓进行单个核细胞悬液的制备。在DSA监视下将采集的单个核细胞混悬液经股动脉行Seldinger法穿刺,穿刺成功后,置入4F动脉鞘,经动脉鞘置入Cobra导管,将导管超选择至闭孔动脉及旋股内外侧动脉,平均注入单个核细胞悬液。术后定期随访症状变化情况,1年后复查X射线或CT,随访疼痛、关节活动度等情况。实验评估:①疼痛指数:无疼痛症状为3分,Harris髋关节评分疼痛分级A级;时有隐痛2分,Harris髋关节评分疼痛B级;轻度疼痛为1分,Harris髋关节评分疼痛C级;中度疼痛为0分,Harris髋关节评分疼痛D级。②功能指数:髋关节屈、伸、展、收、旋转度评分达Harris髋关节活动范围评分4～5分为3分;3～4分为2分;2～3分为1分;小于2分为0分。③X射线平片指数:股骨头形态无变化,应力骨小梁清晰,坏死区明显缩小为3分;坏死区略缩小为2分;治疗前后无明显变化为1分;坏死区扩大为0分。④血管指数:治疗后旋股内、外侧动脉及其分支增粗、增多,延长1cm以上者3分;1～0.5cm者2分;小于0.5cm者1分,无变化者0分。结果:54例患者均完成疼痛症状、关节功能及影像学随访1年。①术后12个月复查疼痛消失9髋,缓解61髋,无缓解21髋,缓解率为76.9%。②关节功能缓解33髋,无缓解58髋,缓解率为36.3%。③1年后X射线平片或CT、MRI示股骨头区可见不同程度的股骨头坏死区骨质密度改变,坏死区有吸收、缩小,股骨头形态变圆滑规整,改善28髋,无缓解或加重63髋,缓解率为30.1%。④12例24髋完成术后12个月复查股骨头供血动脉数字减影血管造影,好转18髋,好转率为72.2%。结论:经介入途径移植自体骨髓单个核细胞治疗股骨头坏死损伤小,可缓解临床相关症状。

关键词：股骨头缺血性坏死骨髓干细胞移植

Protein arginine methyltransferase-6 regulates heterogeneous nuclear ribonucleoprotein-F expression and is a potential target for the treatment of neuropathic pain

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Neural Regeneration Research 2025年第9期20卷 2682-2696页

作者： Xiaoyu Zhang Yuqi Liu Fangxia xu Chengcheng Zhou Kaimei Lu Bin Fang Lijuan Wang Lina Huang Zifeng xu Department of Anesthesiology International Peace Maternity and Child Health HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina Department of Anesthesiology Shanghai General HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina Shanghai Key Laboratory of Embryo Original Diseases ShanghaiChina

Protein arginine methyltransferase-6 participates in a range of biological functions,particularly RNA processing,transcription,chromatin remodeling,and endosomal ***,it remains unclear whether protein arginine methyl transferase-6 modifies neuropathic pain and,if so,what the mechanisms of this *** this study,protein arginine methyltransferase-6 expression levels and its effect on neuropathic pain were investigated in the spared nerve injury model,chronic constriction injury model and bone cancer pain model,using immunohistochemistry,western blotting,immunoprecipitation,and label-free proteomic *** results showed that protein arginine methyltransferase-6 mostly co-localized withβ-tubulinⅢin the dorsal root ganglion,and that its expression decreased following spared nerve injury,chronic constriction injury and bone cancer *** addition,PRMT6 knockout(Prmt6~(-/-))mice exhibited pain ***,the development of spared nerve injury-induced hypersensitivity to mechanical pain was attenuated by blocking the decrease in protein arginine methyltransferase-6 ***,when protein arginine methyltransferase-6 expression was downregulated in the dorsal root ganglion in mice without spared nerve injury,increased levels of phosphorylated extracellular signal-regulated kinases were observed in the ipsilateral dorsal horn,and the response to mechanical stimuli was ***,protein arginine methyltransferase-6 appeared to contribute to spared nerve injury-induced neuropathic pain by regulating the expression of heterogeneous nuclear ***,protein arginine methyltransfe rase-6-mediated modulation of hete rogeneous nuclear ribonucleoprotein-F expression required amino atids 319 to 388,but not classical H3R2 *** findings indicated that protein arginine methyltransferase-6 is a potential therapeutic target fo r the treatment of peripheral neuro pathic pain.

关键词： dorsal root ganglion heterogeneous nuclear ribonucleoprotein F neuropathic pain protein arginine methyltransferase-6 sensory neurons

Study on Coastal Protection Engineering in the Area of Abandoned Yellow River Mouth

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China Ocean Engineering 1998年第2期13卷 223-234页

作者： Zhang, yf Yu, GH Sheng, ZC xu, M Nanjing Hydraul Res Inst River & Harbour Dept Nanjing 210024 Peoples R China

This paper reviews the history of coast evolution and coastal protection in the area of the abandoned Yellow River mouth in Jiangsu Province, analyzes the erosion characteristics and dynamic environment, evaluates the efficiency of coastal protection engineering works and designs the dimensions of the main seadike and offshore submerged dam. A gerneral scheme of shore protection for this area is proposed.

关键词： coastal protection coastal erosion wave calculation main seadike groin offshore submerged dam

Helicobacter pylori vacA genotypes and cagA status and their relationship to associated diseases

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World Journal of Gastroenterology 2000年第4期6卷 605-607页

作者： Hou P Tu ZX xu GM Gong yf Ji XH Li ZS Changhai Hosp Dept Gastroenterol Shanghai 200433 Peoples R China

Helicobacter pylori (H. pylori ) is a major causativebacterium of chronic gastritis, peptic ulcer and mucosaassociated lymphoid tissue lymphoma in humans, and associated with an increased risk of gastric cancer[1 -8]. An important virulant factor of H. pylori is the vacuolating cytotoxin ( VacA ) encoded by vacA that induces cytoplasmic vacuolation in target cells both in vitro and in vivo[9-11]. VacA is produced as a 140 kDa precursor which contains an N-terminal signal peptide and an approximately 33 kDa C-terminal outer membrance exporter. The precursor is cleaved at both N-terminal and C-terminal and secreted into the extracellular milieu as a 95 kDa mature protein. The mature protein futher undergoes specific cleavage to yield 37 kDa and 58 kDa subunits[12-14] Although vacA is present in all H. pylori strains, only about 50% to 60% of strains can induce vacuolation of epithelial cells as assessed by the HeLa cell assay. vacA shows considerable genetic variation in H. pylori isolated from all over the world and contains at least two variable regions. The s region exists as sl or s2 allelic types. Among type sl strains, subtypes sla and slb have been identified. The m region occurs as ml or m2 allelic types. Specific vacA genotype of H. pylori strains are associated with the production of the cytotoxin in vitro, epithelial damage in vivo, and clinical consequences[15-27]. The other virulant factor is the cytotoxin-associated protein (CagA) encoded by the cytotoxin-associated gene (cagA). The cagA gene is present in about 60% to 70% of strains and all of these strains express the cagA. The presence of cagA is also associated with the production of the cytotoxin in vitro, and clinical outcome[24-30]. The aim of this study was (i) to identify vacA genotypes and cagA status of H. pylori isolated from Chinese patients; (ii) to evaluation the relatioship beween vacA genotypes, cagA status and related gastroenterological disorders.

关键词： Helicobacter pylori gastritis peptic ulcer stomach neoplasms genotype polymerase chain reaction

Fractal scaling of effective diffusion coefficient of solute in porous media

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Journal of Environmental Sciences 2001年第2期13卷 170-172页

作者： Liu, JG Nie, yf Tsinghua Univ Dept Environm Sci & Engn Beijing 100084 Peoples R China

Fractal approach is used to derive a power law relation between effective diffusion coefficient of solute in porous media and the geometry parameter characterizing the media. The results are consistent with the empirical equations analogous to Archie`s law and are expected to be applied to prediction of effective diffusion coefficient. Key words: diffusion; effective diffusion coefficient; fractal; porous media.

关键词： diffusion effective diffusion coefficient fractal porous media

Fracture Mechanism of UNS G10190 Steel in Hydrochloric Acid Containing Ferric Ion

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Journal of Materials Science & Technology 1996年第3期12卷 223-226页

作者： Cheng, yf Du, YL State Key Lab. of Corrosion Science and Protection Institute of Corrosion and Protection of Metals Chinese Academy of Sciences Shenyang 110015 China

In the paper, a new kind of electrochemical sensor was used for measuring the permeation rate of atomic hydrogen (H permeation current I) through the sensor in 5% HCl solution containing ferric ion with different concentrations. The susceptibility of brittle fracture and the fracture mechanism of UNS G10190 steel in the solution were studied by slow strain rate tensile technique (SSRT), scanning electron microscope (SEM) and cathodic and anodic polarization. The embrittlement of the steel in the solution was expressed as index of embrittlement (F%). The results showed that the fracture mechanism of the steel in 5%HCl aqueous solution containing ferric ion would be changed from hydrogen induced cracking (HIC) to anodic dissolution in nature with the increase of the concentration of ferric ion in the solution.

关键词： UNS Fracture Mechanism of UNS G10190 Steel in Hydrochloric Acid Containing Ferric Ion

The isotopic abundance and atomic weight of natural uranium

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Chinese Science Bulletin 1997年第10期42卷 814-817页

作者： Liu, yf Fu, SC China Institute of Atomic Energy Beijing China

THERE are three radioactive nuclides of uranium in nature,234U,235U and 238*** isotopicabundance and atomic weight of unanium was reported *** 1983,the International U-nion of Pure and Applied Chemistry（IUPAC）gave a review of the published *** resultsof the review showed the abundance of uraniums,234U:0.000 055（5）;235U:0.007 200（12）;238U:0.992 745（60）,the atomic weight of uranium,A,（U）=238.028 9（1）.

关键词： isotopic abundance of uranium atomic weight of uranium mass spectrometric measurement.

Estimation of Water Particle Velocities by Empirical Transfer Function

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China Ocean Engineering 1997年第4期12卷 393-410页

作者： Chiu, yf Kuo, YY Inst Harbor & Marine Technol Wuchi Coastal Engn Div Taichung Taiwan Natl Chiao Tung Univ Dept Civil Engn Hsinchu Taiwan

In previous and this studies it appears that the linear and nonlinear wave theory-can not accurately and easily predict the water particle velocities: Therefore, different from the theoretical considerations, in this study we have attempted to determine the transfer function empirically. Laboratory experiments were performed under various wave conditions. The empirical formulas of the transfer function of the wave height, angular frequency and water particle velocity were obtained on the basis of these test data by dimensional analysis and regression analysis. In intermediate and deep water depth conditions, the transfer function was only a function of a nondimensional parameter which is composed of the angular frequency, the depth of the velocity gauge under the still water level, water depth and the acceleration of gravity. Finally, the empirical formulas were compared with experimental data and observational data form present and Cavaleri's (1978) studies. The empirical formulas were found to be in sufficient correltion with these data.

关键词： water particle velocity empirical transfer function