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无人机集群分组编队自适应跟踪-包含控制

北京航空航天大学学报 2024年第1期50卷 97-109页

作者：张毅于浩杨秀霞姜子劼王晨蕾海军航空大学烟台264001

针对无人机(UVA)集群分组编队跟踪-包含控制问题,建立包括分组协同、跟踪-包含的分层协同控制架构。为突出集群系统的顶层规划能力,提出基于队形参数组的分组协同控制策略,克服了现有多编队控制方法难以实现队形变换的缺陷;在考虑组间... 详细信息

针对无人机(UVA)集群分组编队跟踪-包含控制问题,建立包括分组协同、跟踪-包含的分层协同控制架构。为突出集群系统的顶层规划能力,提出基于队形参数组的分组协同控制策略,克服了现有多编队控制方法难以实现队形变换的缺陷;在考虑组间通信耦合的基础上,设计自适应分组编队跟踪-包含控制律,克服了控制输入对Laplacian矩阵特征值的依赖,并给出集群系统实现期望队形的可行性约束;对控制输入引入饱和约束,并进一步推导得到输入饱和约束下系统稳定的充要条件,避免了集群的大幅机动行为。研究结果表明:所提策略能够实现无人机集群分组编队的跟踪-包含控制。

关键词：分组编队跟踪-包含控制协同控制输入饱和队形变换

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气相色谱法测定植物源性食品中8种农药残留

现代食品 2024年第7期30卷 180-183页

作者：杨秀虾南平市食品药品检验检测中心福建南平353000

目的:建立植物源性食品中敌百虫、特丁硫磷、杀扑磷、硫环磷、丙溴磷、伏杀硫磷、灭线磷、甲基硫环磷残留量的气相色谱法测定方法。方法:试样以乙腈为提取溶剂,经QuEChERS方法提取净化后,用气相色谱法对植物源性食品中8种农药残留量进... 详细信息

目的:建立植物源性食品中敌百虫、特丁硫磷、杀扑磷、硫环磷、丙溴磷、伏杀硫磷、灭线磷、甲基硫环磷残留量的气相色谱法测定方法。方法:试样以乙腈为提取溶剂,经QuEChERS方法提取净化后,用气相色谱法对植物源性食品中8种农药残留量进行测定。结果:被测定的8种目标物组分在各线性范围内均具有良好的线性关系,相关系数为0.9988~0.9997,在低、中、高3个浓度加标水平的平均回收率为70.0%~107.5%,相对标准偏差为1.8%~8.4%(n=6)。结论:该方法快速、准确性好、干扰较少、精密度高,可应用于植物源性食品中上述农药残留量的快速筛查与定量分析。

关键词：气相色谱法农药残留植物源性食品

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Obstacle avoidance method of three-dimensional obstacle spherical cap

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Journal of Systems Engineering and Electronics 2018年第5期29卷 1058-1068页

作者： yang xiuxia ZHANG Yi ZHOU Weiwei Naval Aviation University Yantai 264001China

Focusing on obstacle avoidance in three-dimensional space for unmanned aerial vehicle(UAV), the direct obstacle avoidance method in dynamic space based on three-dimensional velocity obstacle spherical cap is proposed, which quantifies the influence of threatening obstacles through velocity obstacle spherical cap parameters. In addition, the obstacle avoidance schemes of any point on the critical curve during the multi-obstacles avoidance are given. Through prediction, the insertion point for the obstacle avoidance can be obtained and the flight path can be replanned. Taking the Pythagorean Hodograph(PH) curve trajectory re-planning as an example, the three-dimensional direct obstacle avoidance method in dynamic space is tested. Simulation results show that the proposed method can realize the online obstacle avoidance trajectory re-planning, which increases the flexibility of obstacle avoidance greatly.

关键词： three-dimensional direct space obstacle avoidance velocity obstacle cone velocity obstacle spherical cap

Siphon-Specific Expression of an Actin Encoding Gene Is Regulated by Six1/2 in Ciona savignyi

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Journal of Ocean University of China 2022年第6期21卷 1597-1606页

作者： YUE Wenxuan QIAO Jinghan yang xiuxia DONG Bo Sars-Fang Center MOE Key Laboratory of Marine Genetics and BreedingCollege of Marine Life SciencesOcean University of ChinaQingdao 266003China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China Institute of Evolution and Marine Biodiversity Ocean University of ChinaQingdao 266003China

Actin is a ubiquitous protein and plays essential roles on cellular structure maintenance and cellular motility in both muscle and non-muscle *** genes encoding muscle actin have been identified from the ascidians,including those expressed in the larval tail muscle,the adult body-wall muscle,and adult heart *** this study,a novel striated non-tail muscle actin gene was identified from the RNA-seq data of Ciona savignyi *** analysis,alignment of the N-terminal amino acid sequences and comparation of diagnostic residues provided evidence that it had high similarity with vertebrate cardiac and skeletal muscle *** situ hybridization and promoter-driven GFP reporter assay revealed that it was specifically expressed in the primordia of the oral and atrial *** hereby defined it as siphon-specific muscle actin coding gene(Cs-SMA).A 201 bp(−1350 bp to−1150 bp)sequence containing T-box and Six1/2 binding motif within the upstream region of Cs-SMA confined the expression of GFP in the siphons of electroporated ***1/2 binding motif was experimentally confirmed to play indispensable role in controlling the siphon-specific expression of *** tissue-specific expression of Cs-SMA in the siphon primordia indicated its potential crucial roles in Ciona embryogenesis and organogenesis.

关键词： Ciona savignyi actin encoding gene siphon Six1/2 expression regulation

Establishment and Characterization of a New Marine Fish Cell Line from Ovary of Barfin Flounder(Verasper moseri)

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Journal of Ocean University of China 2015年第6期14卷 1105-1110页

作者： XU Xiaohui FAN Tingjun JIANG Guojian yang xiuxia College of Marine Life Sciences Ocean University of China

A novel continuous ovary cell line from barfin flounder(Verasper moseri)(BFO cell line) was established with its primitive application in transgenic expression demonstrated in this study. Primarily cultured cells grew well at 22℃ in Dulbecco's modified Eagle medium/F12 medium(DMEM/F12, 1:1; p H 7.2) supplemented with 20% fetal bovine serum(FBS), carboxymethyl chitooligosaccharide, basic fibroblast growth factor(b FGF) and insulin-like growth factor-I(IGF-I). The primary BFO cells in fibroblastic morphology proliferated into a confluent monolayer about 2 weeks later, and were able to be subcultured. Impacts of medium and temperature on the growth of the cells were examined. The optimum growth was found in DMEM/F12 with 20% FBS and at 22℃. The BFO cells can be continuously subcultured to Passage 120 steadily with a population doubling time of 32.7 h at Passage 60. Chromosome analysis revealed that 72% of BFO cells at Passage 60 maintained the normal diploid chromosome number(46) with a normal karyotype of 2st+44t. The results of gene transformation indicated that green fluorescence protein(GFP) positively expressed in these cells after being transformed with pc DNA3.1-GFP. Therefore, a continuous and transformable BFO cell line was successfully established, which may serve as a useful tool for cytotechnological manipulation and transgenic modification of this fish.

关键词： barfin flounder ovary cell cell line gene transformation green fluorescence protein GFP Verasper moseri

裂隙灯显微镜示教联合督导式教学门诊在全科住院医师眼科规范化培训中应用效果的探讨

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中华全科医师杂志 2024年第9期23卷 964-968页

作者：杨程成郑晖刘苹苹赵晓静刘洋杨秀霞中山大学附属第五医院眼科珠海519000

目的探讨裂隙灯显微镜示教联合督导式教学门诊在全科住院医师眼科规范化规培中的实践效果。方法该研究为随机对照试验。纳入2021年3月至2023年3月于中山大学附属第五医院眼科参加规范化培训的全科医师。采用抓阄法将入选学员随机分为联... 详细信息

目的探讨裂隙灯显微镜示教联合督导式教学门诊在全科住院医师眼科规范化规培中的实践效果。方法该研究为随机对照试验。纳入2021年3月至2023年3月于中山大学附属第五医院眼科参加规范化培训的全科医师。采用抓阄法将入选学员随机分为联合教学组和传统教学组。基线时入选学员先进行入科理论考核。传统教学组教学过程中教师起主导作用,学员在教师指导下进行观察和记录。联合教学组采用裂隙灯显微镜示教联合督导式教学门诊法进行教学。培训后两组学员统一接受出科考核,记录学员理论知识得分、门诊诊疗能力考核得分、综合评价得分以及总成绩。同时,通过调查问卷评估学员及带教老师对教学过程的满意度。结果共入选学员60名,男性28名(47%),年龄23.0(23.0,25.0)岁,其中传统教学组29名,联合教学组31名。两组学员的性别构成、年龄、入科理论考试成绩差异均无统计学意义(均P>0.05)。培训结束后,联合教学组学员出科理论知识得分、门诊诊疗能力得分及总成绩均高于传统教学组(均P<0.05)。联合教学组学员教学满意度各项得分均高于传统教学组(均P<0.05),联合教学组带教教师对学员的满意度各项得分亦均高于传统教学组(均P<0.05)。结论裂隙灯显微镜示教联合督导式教学门诊可提高全科住院医师眼科规范化培训效果。

关键词：教学眼科学全科医师裂隙灯显微镜示教督导式教学门诊

以病房示教为主联合TBL教学法在眼科教学中的探索

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中国继续医学教育 2024年第7期16卷 82-86页

作者：杨程成杨秀霞刘苹苹赵晓静郑晖刘洋中山大学附属第五医院眼科广东珠海519000

目的眼科学是临床专业学生的必修内容,其内容专业性强、学时相对有限、教学难度大。在眼科教学过程中改进教学方法并提高教学效率已经成为眼科学教学研究的重要课题。方法选取2021年11月—2022年5月于中山大学附属第五医院眼科见习的79... 详细信息

目的眼科学是临床专业学生的必修内容,其内容专业性强、学时相对有限、教学难度大。在眼科教学过程中改进教学方法并提高教学效率已经成为眼科学教学研究的重要课题。方法选取2021年11月—2022年5月于中山大学附属第五医院眼科见习的79名中山大学临床医学系本科学生为研究对象。随机分为对照组和研究组,对照组39名,研究组40名。对照组授课方式为传统教学法,研究组授课采用以病房示教为主联合以团队为基础的教学法(team-based learning,TBL)。教学结束后进行理论及技能考试对教学效果进行评价,采用问卷调查的方法评估学生对教学模式的满意度。结果研究组病例分析成绩[(89.04±4.03)分]优于对照组[(80.29±4.51)分],差异有统计学意义(P0.05)。教学满意度评价显示,研究组中对眼科疾病的整体认识、理论知识掌握程度及提高临床思维能力、拓宽知识面增强横向联系及纵向认知、提高眼科学专业学习兴趣、提升学习效率、提高沟通能力、提高团队合作精神的满意程度更高,差异有统计学意义(P<0.05)。结论病房示教为主联合TBL教学法的教学模式是眼科学临床教学方法改革的积极探索,可显著提高见习生对临床眼科疾病的识别及诊疗能力,加强眼科见习生对眼科疾病的掌握程度。

关键词：病房示教以团队为基础眼科教学本科见习教学改革传统教学

Recent developments and progress of inorganic photo-stimulated phosphors

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Journal of Rare Earths 2019年第7期37卷 679-690页

作者： Xiaotong Fan Zhichao Liu xiuxia yang Wenbo Chen Wei Zeng Shuyu Tian Xue Yu Jianbei Qiu Xuhui Xu College of Materials Science and Engineering Kunming University of Science and TechnologyKunming 650093China Engineering Research Center of New Energy Storage Devices and Applications Chongqing University of Arts and SciencesChongqing 401260China College of Chemistry and Chemical Engineering Northwest Normal UniversityLanzhou 730070China

Photo-stimulated luminescence(PSL) is the process in which trapped charges are released by photons and produce luminescence through recombination. The variegated optical characteristics of photostimulated phosphors(PSPs) have drawn increasing attention and a large body of work encompassing mechanism and application of PSPs has been addressed. The optical data storage capacity resulting from abundant defect states enables PSPs to be applied to information storage. Moreover, PSPs provide potential application for anti-counterfeiting, as color changes due to the tunneling process. Recently, near infrared(NIR) light PSPs have been developed, exhibiting enormous potential for in vivo bio-imaging, as the stable and high noise-signal ratio characteristic of PSL. In this review, we devote to introducing the development and process of PSPs, and the challenge and future advance have also been demonstrated.

关键词： Photo-stimulated phosphors Traps Optical data storage Anti-counterfeiting Bio-imaging Rare earths

Development of an Inactivated Iridovirus Vaccine Against Turbot Viral Reddish Body Syndrome

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Journal of Ocean University of China 2012年第1期11卷 65-69页

作者： FAN Tingjun HU Xiuzhong WANG Liyan GENG Xiaofen JIANG Guojian yang xiuxia YU Miaomiao Department of Marine Biology College of Marine Life SciencesOcean University of ChinaQingdao 266003P.R.China

Turbot(Scophthalmus maximus L.) reddish body iridovirus(TRBIV) was propagated in turbot fin cells(TF cells) and inactivated as the TRBIV vaccine with its protection efficiency evaluated in this *** cells were cultured in 10% bovine calf serum(BCS)-containing MEM medium(pH7.0) at 22℃,in which TRBIV propagated to a titer as high as 105.6 TCID50 *** TRBIV was inactivated with 0.1% formalin and formulated with 0.5% aluminum *** inactivated vaccine caused neither cytopathogenic effect(CPE) on TF cells nor pathogenic effect on *** being administered with the vaccine twice via muscle injection,the turbot developed high-tittered TRBIV neutralizing antibodies in a dose-dependent *** vaccine protected the turbot from dying with an immunoprotection rate of 83.3% as was determined via subcutaneous vaccination in the laboratory and 90.5% via bath vaccination in turbot farms,*** inactivated vaccine was very immunogenic,efficiently preventing tur-bot from *** holds the potential of being applied in aquaculture.

关键词： turbot reddish body iridovirus turbot fin cell inactivated vaccine Scophthalmus maximus