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Comparison of Microdroplet Digital PCR Assays with Real-time Fluorescence Quantitative PCR for Clostridioides difficile Detection

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Biomedical and Environmental Sciences 2023年第7期36卷 653-657页

作者： YIN Cai Hong SONG Zhan yun LIU Xing Xing WANG Xiao Mu WANG Ying gao Cheng Cheng SONG xiu Ling FENG Xin College of Public Health Jilin UniversityChangchun 130021JilinChina Changchun customs Technology Center Changchun130062JilinChina CollegeofVeterinaryMedicine JilinUniversityChangchun130062JilinChina College of Basic Medical Sciences Jilin UniversityChangchun 130021JilinChina

Clostridioides difficile(***),is an anaerobic Gram-positive bacterium found in the human *** use of antibacterial drugs can result in *** infection(CDI)[1],which is one of the most important hospital-acquired diseases affecting all ***-and-culture-based identification and the cell cytotoxicity neutralization assay(CCNA)are considered the“gold standard”for detecting toxins or pathogenic strains[2],with long and time-consuming incubation *** quantitative polymerase chain reaction(PCR)is commonly used in clinical laboratory tests[3].However,the sensitivity,accuracy,and resolution of detection are limited by low-copy target gene molecules and subtle differences in template *** *** is distributed in feces in the natural environment and the sample matrix is complex,it is difficult to detect pathogens at low *** quantifying *** and implementing effective measures to combat *** infections are of utmost importance.

关键词： intestine drugs template

Simplified Scheme for Teleportation of a Multipartite Quantum State Using a Single Entangled Pair

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Communications in Theoretical Physics 2009年第2期51卷 235-238页

作者： YAN Li-Hua gao yun-Feng School of Physical Science and Information Engineering Liaocheng University Liaocheng 252059 China School of Medium and Communications Liaocheng University Liaocheng 252059 China

Abstract A simple scheme for teleporting an unknown M-qubit cat-like state is proposed. The steps of this scheme can be summarized simply： disentangle-teleport-reconstruct entanglement. If proper unitary operations and measurements from senders are given, the teleportation of an unknown M-qubit cat-like state can be converted into single qubit teleportation. In the meantime, the receiver should also carry out right unitary operations with the introduction of appropriate ancillary qubits to confirm the successful teleportation of the demanded entangled state. The present scheme can be generalized to teleport an unknown M-quNit state, i.e., an M-quNit state can be teleported by a single quNit entangled pair.

关键词： teleportation EPR pair M-qubit cat-like state quNit entangled pair M-quNit cat-like state

Hydrothermal Synthesis and Crystal Structure of Manganese(Ⅱ) Coordination Polymer Assembled by 4-Sulfophthalate and 4,4′-Bipyridine

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Chemical Research in Chinese Universities 2007年第1期23卷 105-108页

作者： WANG Qing-wei LI xiu-mei gao Guang gang Department of Chemistry Jilin Normal University Siping 136000 P. R. China Department of Chemistry Tonghua Teachers College Tonghua 134002 P.R. China

The rational design and synthesis of metal-directed supramolecular framework compounds have received much attention in coordination chemistry because of their potential applications in catalysis, molecular selection, nonlinear optics, ion exchange, and microelectronics. Many high-dimensional coordination complexes have been designed and prepared through molecular self-assembly processes. The open metal organic framework can be produced via two kinds of interactions, i. e. , coordinate covalent bonds and weaker intermolecular forces. For this reason, the most efficient means to synthesize these compounds is to establish possible connections among different units.

关键词： Hydrothermal synthesis Crystal structure Supramoleeular network Mixed ligand

Organic matter pores in the chang 7 lacustrine shales from the Ordos Basin and its effect on reflectance measurement

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Petroleum Science 2023年第1期20卷 60-86页

作者： Peng Pang Hui Han xiu-Cheng Tan Shi-Meng Ren Chen Guo Lin Xie Ling-Li Zheng Hai-Hua Zhu Yuan gao yun-Hui Xie School of Geoscience and Technology Southwest Petroleum UniversityChengduSichuan610500China Natural Gas Geology Key Laboratory of Sichuan Province Southwest Petroleum UniversityChengduSichuan610500China Shale Gas Industry Development Institute of Sichuan Province ChengduSichuan610500China The First Branch Mud Logging Company CNPC Bohai Drilling Engineering Co.Ltd.Tianjin300457China State Key Laboratory of Oil and Gas Reservoir Geology and Exploration Southwest Petroleum UniversityChengduSichuan610500China Beijing Center for Physical and Chemical Analysis Beijing100089China Nursing College Guizhou University of Traditional Chinese MedicineGuiyangGuizhou520002China

To quantify the pore characteristics of various macerals in Chang 7 lacustrine shales,macerals were effectively identified according to their optical and morphological characteristics,and the nanoscale pore structure of macerals was observed by scanning electron ***,the reflectances of different positions in the same pieces of vitrinite or solid bitumen with heterogeneous pores development were *** results showed that the average contents of sapropelinite,liptinite,vitrinite,inertinite and solid bitumen are 42.7%,8.7%,13.6%,13.8% and 21.2%,respectively,which suggests that the source of the organic matter of the Chang 7 shales is a mixed source *** organic pores of Chang 7 shales are enriched,and the pore shapes are mostly round or *** pore size of organic pores has a wide distribution,mainly concentrate in the range of 100-400 nm,and the average plane porosity of organic pores is 10.13%.The size order of the organic pores in various macerals is:solid bitumenfusinite>bituminite>solid bitumen>vitrinite>*** pores are mainly contributed by bituminite,solid bitumen and *** plane porosity of bituminite increases with *** the process of thermal evolution,the plane porosity of fusinite is distributed in the two ranges of 20%-28% and 1%-7%.The former is mainly the primary pores of the fusinite itself,and the latter is the secondary pores formed in the thermal *** for the organic pores of other macerals,no obvious thermal evolution law was ***,the surface imperfections of vitrinite or solid bitumen is enhanced by the enrichment of organic pores(an increase in pore size or pore number),which may result in the underestimation of their reflectances.

关键词： Ordos Basin Chang 7 member OM pore Macerals Differential developmental characteristics Reflectance

Synthesis of Methyl-substituted Phthalazinone-based Aromatic Poly(amide imide)s

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Chinese Chemical Letters 2002年第9期13卷 824-825页

作者： xiu Ling ZHU Xi gao JIAN Lian Zhou CHEN Department of Polymer Science&Materials Dalian University of TechnologyDalian 116012

A series of novel aromatic poly ( amide imide)s containing phthalazinone moieties were prepared from 2-(4-aminophenyl)-4-[3-methyl-4-(4-aminophenoxy)-2,3-phthalazinone-1], a novel diamine 1 with four diimide-dicarboxylic acids by Yamazaki phosphorylation method with the inherent viscosity of 0.36~0.65 dL/g. These polymers had high glass transition temperatures above 300C and they lost 10% weight between 426~475C in N2. The structure of diamine 1 and the polymers was confirmed by IR, 1H NMR and MS. The obtained polymers were readily soluble in polar solvents such as NMP, m-cresol etc. and easily cast into tough, flexible films. The X-ray indicated that they are all amorphous.

关键词： Aromatic poly(amide imide) phthalazinone synthesis heat-resistance.

Positive psychological intervention for anxiety, depression and coping in subjects addicted to online games

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World Journal of Clinical Cases 2021年第14期9卷 3287-3293页

作者： xiu-Jun gao Ji-Jun Sun Mei Xiang Department of Psychiatry Hangzhou Seventh People’s HospitalHangzhou 310013Zhejiang ProvinceChina Department of Elderly Psychiatry Hangzhou Seventh People’s HospitalHangzhou 310013Zhejiang ProvinceChina

BACKGROUND Addiction to online games is not *** patients usually refuse to take medications and present with affective symptoms such as anxiety,depression and negative coping *** intervention based on positive psychology is a promising treatment for such *** To evaluate the effect of positive psychological intervention on anxiety,depression and coping in people addicted to online *** This self-controlled study included 89 people addicted to online games,who received treatment at Hangzhou Seventh People’s Hospital,Hangzhou,China in *** Hamilton Anxiety Scale,Hamilton Depression Scale and Trait Coping Style Questionnaire were administered to evaluate the anxiety,depression and coping style among these *** intervention based on positive psychology was provided for 6 wk followed by another *** results were compared against those from the previous *** After 6 wk of psychological intervention,89 people achieved a significant improvement in the Hamilton Anxiety Scale and Hamilton Depression Scale-24 *** score for positive coping style in Trait Coping Style Questionnaire was significantly improved,while that of the negative coping style decreased significantly(P<0.05).CONCLUSION Psychological intervention based on positive psychology alleviated affective symptoms,such as anxiety and depression,in subjects addicted to online *** intervention corrected negative coping style,thereby improving mental health.

关键词： Online game addiction Positive psychology Anxiety Depression Coping style Psychological intervention

Novel Polyamides Containing Biphenyl-phthalazinone Moieties

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Chinese Chemical Letters 2001年第6期12卷 517-518页

作者： xiu Ling ZHU Xi gao JIAN Rui Ling WANG Department of Polymer Science&Materials Dalian University of TechnologyDalian 116012

A novel diamine monomer 2H-(4-aminophenyl)-4-[4-(4-aminophenoxy) biphenyl]phthalazinone 1 containing aza hetero cycle was synthesized and utilized for preparation of new high molecular weight aromatic polyamides with various aromatic dicarboxylic acids with the inherent viscosity of 0.50 similar to 1.62 g/dL. These polyamides had rather high glass transition temperatures of 327 similar to 356 degreesC and their structure was confirmed by IR, H-1 NMR and MS. They are readily soluble in polar solvents such as NMP, DMF etc, and easily cast into tough, flexible films.

关键词： Polyarylamide biphenyl-phthalazinone solubility thermostability

Evaluation of the net CO_2 uptake in the Canada Basin in the summer of 2008

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Acta Oceanologica Sinica 2017年第8期36卷 94-100页

作者： SUN Heng gao Zhongyong LU Peng xiu Peng CHEN Liqi Key Laboratory of Global Change and Marine Atmospheric Chemistry of State Oceanic Administration Third Institute of OceanographyState Oceanic Administration State Key Laboratory of Coastal and Offshore Engineering Dalian University of Technology State Key Laboratory of Tropical Oceanography South China Sea Institute of OceanologyChinese Academy of Sciences

The third Chinese National Arctic Research Expedition （CHINARE） was conducted in the summer of 2008. During the survey, the surface seawater partial pressure of CO2 （pCO2） was measured, and sea water samples were collected for CO2 measurement in the Canada Basin. The distribution of pCO2 in the Canada Basin was determined, the influencing factors were addressed, and the air-sea CO2 flux in the Canada Basin was evaluated. The Canada Basin was divided into three regions： the ice-free zone （south of 77°N）, the partially ice-covered zone （77°-80°N）, and the heavily ice-covered zone （north of 80°N）. In the ice-free zone, pCO2 was high （320 to 368 patm, 1 patm=0.101 325 Pa）, primarily due to rapid equilibration with atmospheric CO2 over a short time. In the partially ice-covered zone, the surface pCOs was relatively low （250 to 270 patm） due to ice-edge blooms and icemelt water dilution. In the heavily ice-covered zone, the seawater pCO2 varied between 270 and 300 laatm due to biological COs removal, the transportation of low pCOs water northward, and heavy ice cover. The surface seawater pCO2 during the survey was undersaturated with respect to the atmosphere in the Canada Basin, and it was a net sink for atmospheric CO2. The summertime net CO2 uptake of the ice-free zone, the partially ice-covered zone and the heavily ice-covered zone was （4.14±1.08）, （1.79±0.19）, and （0.57±0.03） Tg/a （calculated by carbon, 1 Tg=10^12 g）, respectively. Overall, the net COs sink of the Canada Basin in the summer of 2008 was （6.5＋1.3） Tg/a, which accounted for 4%-10% of the Arctic Ocean COs sink.

关键词： Canada Basin net CO2 uptake partial pressure of CO2

Enhanced Photothermal Selective Conversion of CO_(2)to CH4 in Water Vapor over Rod-Like Cu and N Co-Doped TiO_(2)

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Chinese Journal of Structural Chemistry 2022年第12期41卷 33-42页

作者： Ji-Chao Wang xiu Qiao Weina Shi Huiling gao Lingchen Guo College of Chemistry and Chemical Engineering Henan Institute of Science and TechnologyXinxiang 453000China School of Chemistry and Materials Engineering Xinxiang UniversityXinxiang 453000China School of Ecology and Environment Zhengzhou UniversityZhengzhou 450052China

Enhancing catalytic efficiency and selectivity is critical issues for CO_(2) *** rod-like Cu/N co-doped TiO_(2) samples(Cu/N-TiO_(2)) were synthesized by the electrospinning-calcination *** substitutional Cu and interstitial N doping not only enhanced visible-light absorption ability,but also formed the Ti(Ⅲ)*** obviously synergistic effect between the photocatalysis and thermalcatalysis appeared for CO_(2) conversion over the 8-Cu/N-TiO_(2) *** 9 h visible-light-illumination at 160℃,the CO,CH4and O_(2) yields reached 49.7,1455.1 and 2910.2μmol/gcat,*** the 7thcycling,the yields of two main CH4and O_(2) products were slightly down by less than 11.5%,and the selectivity of CH_(4) product kept above 98%.Combined with the theoretical surface mode,Cu/N co-doping could promote the adsorption-ability for H_(2)O and CO_(2) molecules and reduce activation-energy for CO_(2)***,the co-doping construction showed a great significance of designing efficient photothermal catalysts for the CO_(2)conversion application.

关键词： CO_(2)conversion photothermal catalysis rod-like TiO_(2) co-doped