检索结果-南通市图书馆

Downregulation of survivin by RNAi inhibits growth of human gastric carcinoma cells

World Journal of Gastroenterology 2007年第8期13卷 1170-1174页

作者： Guo-Ying Miao Qi-Ming Lu xiu-lan zhang Department of Digestion The People's Hospital of Gansu Province Lanzhou 730000 Gansu Province China

AIM: To investigate the inhibitory effect of a specific small survivin interfering RNA (siRNA) on cell proliferation and the expression of survivin in human gastric carcinoma cell line SGC-7901. METHODS: To knockdown survivin expression, a small interfering RNA targeting against survivin was synthesized and transfected into SGC-7901 cells with lipofectamineTM2000. The downregulation of survivin expression at both mRNA and protein levels were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis. Cell proliferation inhibition rates were determined by methyl thiazolyl tetrazolium (MTT) assay. The effect of survivin siRNA on cell cycle distribution and cell apoptosis was determined by flow cytometry (FCM). RESULTS: RNA interference could efficiently suppress the survivin expression in SGC-7901 cells. At 48 h after transfection, the expression inhibition rate was 44.52% at mRNA level detected by RT-PCR and 40.17% at protein level by Western blot analysis. Downregulation of survivin resulted in significant inhibition of tumor cell growth in vitro. The cell proliferation inhibition rates at 24, 48 and 72 h after survivin siRNA and non-siliencing siRNA transfection, were 34.06%, 47.61% and 40.36%, respectively. The apoptosis rate was 3.56% and the number of cells was increased in G0/G1 phase from 38.2% to 88.6%, and decreased in S and G2/M phase at 48 h after transfection. CONCLUSION: Downregulation of survivin results in significant inhibition of tumor growth in vitro. The inhibition of survivin expression can induce apoptosis of SGC-7901 cells. The use of survivin siRNA deserves further investigation as a novel approach to cancer therapy.

关键词： Gastric carcinoma Survivin RNA interference Apoptosis Gene expression

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

TRPC6:an underlying target for human glaucoma

引用

International Journal of Ophthalmology(English edition) 2012年第4期5卷 523-526页

作者： Qian Fan Wen-Bin Huang and xiu-lan zhang Zhongshan Ophthalmic Center State Key Laboratory of Ophthalmology Sun Yat-sen University Guangzhou 510060 Guangdong Province China

Glaucoma is one of the leading causes of visual impairment and blindness worldwide. Of known risk factors for glaucoma, an increased in intraocular pressure is most highly correlated with glaucomatous damage. Irrespective of the cause, apoptosis of the retinal ganglion cells is the eventual outcome. It is widely accepted that glaucoma is a neurodegenerative disease that is strongly correlated with central nervous system disorders, such as Alzheimer's disease. These two disorders also share some similarities in pathogenic mechanisms. Recent studies suggest that the transient receptor potential canonical 6 channel could work together with brain-derived neurotrophic factor to promote neuron survival in brain and retina. In this study, we propose that transient receptor potential canonical 6 may contribute to the pathogenesis of human glaucoma and become a potential therapeutic target.

关键词： glaucoma transient receptor potential canonical 6 channel neuroprotection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cod liver oil: a potential protective supplement for human glaucoma

引用

International Journal of Ophthalmology(English edition) 2011年第6期4卷 648-651页

作者： Wen-Bin Huang Qian Fan and xiu-lan zhang Zhongshan Ophthalmic Center State Key Laboratory of Ophthalmology Sun Yat-sen University Guangzhou 510060 Guangdong Province China

Glaucoma is one of the leading causes of visual impairment and blindness. Improved knowledge of the pathogenesis of this disease has allowed the exploration of new therapeutic methods. In general, elevated intraocular pressure (IOP), oxidative stress, and vascular insufficiency are accepted as the major risk factors for the progression of glaucoma. Many natural compounds have been found beneficial for glaucoma. Nutritional therapies are now emerging as potentially effective in glaucomatous therapy. One nutritional supplement with potential therapeutic value is cod liver oil, a dietary supplement that contains vitamin A and omega-3 polyunsaturated fatty acids (PUFAs). Vitamin A is important for preserving normal vision and it is a well-known antioxidant that prevents the oxidative damage that contributes to the etiology and progression of glaucoma. Vitamin A is also a crucial factor for maintaining the integrity of conjunctival and corneal ocular surfaces, and preventing the impairment of ocular epithelium caused by topical antiglaucomatous drugs. Omega-3 fatty acids are beneficial for glaucoma patients as they decrease IOP, increase ocular blood flow, and improve optic neuroprotective function. In this article, we propose that cod liver oil, as a combination of vitamin A and omega-3 fatty acids, should be beneficial for the treatment of glaucoma. However, further studies are needed to explore the relationship between cod liver oil and glaucoma.

关键词： cod liver oil glaucoma omega-3 fatty acids vitamin A

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Morphological characterization of metal porphyrin tailed with aspirin interacting with bovine serum albumin congeries

引用

Chinese Chemical Letters 2007年第12期18卷 1455-1459页

作者： Kai Wang Lei Wu xiu lan zhang Zao Ying Li College of Chemistry and Molecular Sciences Wuhan University Wuhan 430072 China Hubei Key Laboratory of Novel Reactor ＆ Green Chemical Technology Wuhan 430074 China

The porphyrins tailed with acetylsalicylic acid （ASA） and Zn （or Cu） complexes were prepared. Meanwhile, morphological images, such as shape and size of porphyrins-BSA congeries were observed by using atomic force microscopy （AFM）. The result showed the interaction of BSA and prepared porphyrins led to obvious change of shape and size of BSA congeries.

关键词： Porphyrin Acetylsalicylic acid Bovine serum albumin Atomic force microscopy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Secondary in-the-bag intraocular lens implantation combined with 120-and 360-degree goniotomy in glaucoma following congenital cataract surgery:a case report

引用

International Journal of Ophthalmology(English edition) 2023年第4期16卷 659-662页

作者： Yu zhang Yue Zhou Yun-He Song xiu-lan zhang Wei-Rong Chen State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceGuangdong Provincial Clinical Research Center for Ocular DiseasesGuangzhou 510060Guangdong ProvinceChina

Dear Editor,Glaucoma following cataract surgery(GFCS)is one of the most sight-threatening postoperative complications of pediatric cataract surgery,and often becomes refractory to medical treatment[1].Goniotomy has be... 详细信息

关键词： glaucoma surgery cataract

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ex-PRESS implantation versus trabeculectomy in Chinese patients with POAG:fellow eye pilot study

引用

International Journal of Ophthalmology(English edition) 2017年第1期10卷 56-60页

作者： Wei Wang Min-Wen Zhou Wen-Bin Huang Xin-Bo Gao xiu-lan zhang Zhongshan Ophthalmic Center State Key Laboratory of Oph-thalmologySun Yat-Sen UniversityGuangzhou 510060Guangdong ProvinceChina

AIM： To compare the outcomes of Ex-PRESS implantation in one eye versus trabeculectomy with mitomycin C in the fellow eye in Chinese patients with primary open-angle glaucoma （POAG）. METHODS： This was a prospective, non-randomized comparative study. Forty-eight eyes of 24 patients with bilateral POAG necessitating surgery were included and underwent Ex-PRESS implantation under the scleral flap in one eye and trabeculectomy in the other eye according to patients’ choice. Primary outcome measures included mean intraocular pressure （IOP） and success rate. Secondary outcome measures were aqueous flare, postoperative medication use, visual acuity, and incidence of ***： All 24 patients finished a 1-year follow-up. Both groups maintained significant reductions in IOP after surgery throughout the follow-up period. At any point in time, the IOP of the two groups did not differ significantly. The Kaplan-Meier survival curve analysis showed no significant differences in success between the two groups （P=0.289）. The mean number of anti-glaucoma medicines and visual acuity in both groups were not significantly different. Eyes with Ex-PRESS implantation had lower aqueous flare values on days 1 and 3 （both P〈0.05）. Instances of early postoperative hypotony and choroidal effusion were significantly fewer in frequency after Ex-PRESS implantation under the scleral flap compared with those after trabeculectomy （P〈0.001）. CONCLUSION： Ex-PRESS is comparable to trabeculectomy in terms of IOP, success rate, number of glaucoma medications used, and visual acuity. However, Ex-PRESS resulted in fewer cases of inflammation and a lower rate of complications.

关键词： Ex-PRESS trabeculectomy primary open-angle glaucoma Chinese

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Detection of dopamine using self-assembled diazoresin/single-walled carbon nanotube modified electrodes

引用

Chinese Chemical Letters 2014年第4期25卷 523-528页

作者： Bing Yu Hua Yuan Yi-Ying Yang Hai-Lin Cong Tian-Zi Hao Xiao-Dan Xu xiu-lan zhang Shu-Jing Yang Li-Xin zhang Laboratory for New Fiber Materials and Modern Textile Growing Base for State Key LaboratoryCollege of Chemical EngineeringQingdao University Collaborative Innovation Center for Marine Biomass Fibers Materials and Textiles of Shandong ProvinceQingdao University

Ultrathin films of diazoresin （DR）/single-walled carbon nanotube （SWNT） were fabricated on thioglycollic acid （TGA） decorated gold （Au） electrodes by the self-assembly method combined with the photocrosslinking technique. The electrochemical behavior of dopamine （DA） at the DR/SWNT modified electrodes was studied using the cyclic voltammetry （CV） and differential pulse voltammetry （DPV） methods. Under the optimal conditions, a linear CV response to DA concentration from 1 p, mol/L to 40 μmol/L was observed, and the detection limit of DA was 2.1 ×10-3 μmol/L via the DPV method in the presence of 10μmol/L of uric acid （UA） or 2.5 × 10-3 μmol/L via the DPV method in the presence of 10 μmol/L of ascorbic acid （AA）. Moreover, the modified electrodes exhibited good reproducibility and sensitivity, demonstratinz its feasibilitv for analytical ourooses.

关键词： Self-assembly Single-walled carbon nanotube Diazoresin Dopamine Cyclic voltammetry

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Posterior scleral application of a mitomycin C-soaked sponge during trabeculectomy

引用

International Journal of Ophthalmology(English edition) 2023年第7期16卷 1071-1077页

作者： Kun Hu Yun-He Song Feng-Bin Lin Ying-Zhe zhang Ling Jin Meng-Yin Liang Robert N.Weinreb xiu-lan zhang State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceGuangdong Provincial Clinical Research Center for Ocular DiseasesGuangzhou 510060Guangdong ProvinceChina Hamilton Glaucoma Center Shiley Eye InstituteViterbi Family Department of OphthalmologyUC San DiegoLa JollaCA 92093USA

AIM:To evaluate the safety and efficacy of posterior scleral application(a modified technique)of an antimetabolite mitomycin C-soaked sponge in trabeculectomy for patients with ***:This retrospective study included 101 patients(115 eyes)with glaucoma(aged 12–83y)who underwent trabeculectomy using a modified mitomycin C-soaked sponge placement method.A piece of 3.5×10 mm2 sponge was placed vertically and posteriorly with the long side perpendicular to the *** mitomycin C concentration and exposure time were 0.2–0.5 mg/m L and 1–5min,*** pressure,bestcorrected visual acuity,and hypotensive medications were recorded at baseline and at the final ***,interventions required,and bleb morphology were recorded *** primary outcome was trabeculectomy safety,including complications and bleb morphology;the secondary outcome was the trabeculectomy success ***:At the final follow-up[median 28mo,range 7–67mo and interquartile range(IQR)13mo],the qualified(cumulative)success rate was 93.0%and the complete success rate was 60.0%.No bleb-related complications were *** mean height,extent,and vascularity grades were 0.6±0.9,1.1±0.4,and 2.4±0.9,*** Seidel tests were *** mean posteriority grade was 0.8±***:Trabeculectomy with the long side of a mitomycin C-soaked sponge placed perpendicular to the corneal limbus is safe and effective.

关键词： glaucoma mitomycin C posterior scleral application trabeculectomy bleb morphology

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Possibilities for the synthesis of superheavy element Z=121 in fusion reactions

引用

Nuclear Science and Techniques 2024年第6期35卷 90-100页

作者： Ming-Hao zhang Yu-Hai zhang Ying Zou xiu-xiu Yang Gen zhang Feng-Shou zhang The Key Laboratory of Beam Technology of Ministry of Education College of Nuclear Science and TechnologyBeijing Normal UniversityBeijing 100875China Institute of Radiation Technology Beijing Academy of Science and TechnologyBeijing 100875China School of Physical Science and Technology Guangxi UniversityNanning 530004China National Laboratory of Heavy Ion Accelerator of lanzhou Center of Theoretical Nuclear PhysicsLanzhou 730000China

Based on the dinuclear system model,the calculated evaporation residue cross sections matched well with the current experimental *** synthesis of superheavy elements Z=121 was systematically studied through combinations of stable projectiles with Z=21-30 and targets with half-lives exceeding 50 *** influence of mass asymmetry and isotopic dependence on the projectile and target nuclei was investigated in *** reactions^(254)Es(^(46)Ti,3n)^(297)121 and^(252)Es(^(46)Ti,3n)^(295)121 were found to be experimentally feasible for synthesizing superheavy element Z=121,with maximal evaporation residue cross sections of 6.619 and 4.123 fb at 219.9 and 223.9 MeV,respectively.

关键词： Superheavy nuclei Dinuclear system model Fusion reaction Evaporation residue cross section

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Aqueous proinflammatory cytokines in acute primary angle-closure eyes

引用

International Journal of Ophthalmology(English edition) 2017年第5期10卷 733-737页

作者： Yao-Ming Liu Shi-Da Chen Xing-Yi Li Wen-Bin Huang Fei Li Jia-Wei Wang Ying-Qi Li xiu-lan zhang Zhongshan Ophthalmic Center State Key Laboratory of Ophthalmology Sun Yat-sen University Guangzhou 510060Guangdong Province China

AIM：To evaluate changes of proinflammatory cytokines in aqueous humor of patients with acute primary angleclosure（APAC） and age-related cataracts. METHODS：Twenty eyes of 20 APAC patients and 15 eyes of 15 age-related cataract patients were included in this cross-sectional study. Aqueous humor samples were collected prospectively. The levels of 20 proinflammatory cytokines were evaluated in the aqueous humor of the APAC and cataract patients using the multiplex bead immunoassay technique. Clinical data were collected for correlation ***：Seven of the 20 proinflammatory cytokines included in the magnetic bead panel were detectable in both APAC eyes and cataract eyes：interleukin（IL）-10, IL-12, IL-15, IL-21, IL-6, chemokine（C-C motif） ligand 20, and tumor necrosis factor alpha（TNF-α）. IL-27 was only detectable in APAC eyes. Compared with the cataract eyes, the APAC eyes had significantly elevated concentrations of IL-12（P=0.036）, IL-15（P=0.001）, IL-6（P=0.012）, and IL-27（only detectable in APAC eyes）. Age was positively correlated with IL-12（P=0.022） and IL-6（P=0.037）, and time elapsed between APAC onset and aqueous humor samples collection was positively correlated with IL-15（P=0.037）, IL-27（P=0.040）, and TNF-α（P=0.042）.CONCLUSION：Several proinflammatory cytokines including IL-12,IL-15, IL-6 and IL-27, were elevated in the APAC eyes and may be implicated in its pathologic mechanism.

关键词： acute primary angle-closure aqueous humor inflammation cytokines

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：