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Sodium valproate suppresses abnormal neurogenesis induced by convulsive status epilepticus

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Neural Regeneration Research 2019年第3期14卷 480-484页

作者： Peng Wu Yue Hu xiu-Juan Li Min Cheng li Jiang Department of Neurology Children's Hospital of Chongqing Medical University Chongqing International Science and Technology Cooperation Center for Child Development and Disorders Ministry of Education Key Laboratory of Child Development and Disorders Key Laboratory of Pediatrics in Chongqing

Status epilepticus has been shown to activate the proliferation of neural stem cells in the hippocampus of the brain, while also causing a large amount of neuronal death, especially in the subgranular zone of the dentate gyrus and the subventricular zone. Simultaneously, proliferating stem cells tend to migrate to areas with obvious damage. Our previous studies have clearly confirmed the effect of sodium valproate on cognitive function in rats with convulsive status epilepticus. However, whether neurogenesis can play a role in the antiepileptic effect of sodium valproate remains unknown. A model of convulsive status epilepticus was established in Wistar rats by intraperitoneal injection of 3 mEq/kg lithium chloride, and intraperitoneal injection of pilocarpine 40 mg/kg after 18–20 hours. Sodium valproate(100, 200, 300, 400, 500, or 600 mg/kg) was intragastrically administered six times every day(4-hour intervals) for 5 days. To determine the best dosage, sodium valproate concentration was measured from the plasma. The effective concentration of sodium valproate in the plasma of the rats that received the 300-mg/kg intervention was 82.26 ± 11.23 μg/mL. Thus, 300 mg/kg was subsequently used as the intervention concentration of sodium valproate. The following changes were seen: Recording excitatory postsynaptic potentials in the CA1 region revealed high-frequency stimulation-induced long-term potentiation. Immunohistochemical staining for BrdU-positive cells in the brain revealed that sodium valproate intervention markedly increased the success rate and the duration of induced long-term potentiation in rats with convulsive status epilepticus. The intervention also reduced the number of newborn neurons in the subgranular area of the hippocampus and subventricular zone and inhibited the migration of newborn neurons to the dentate gyrus. These results indicate that sodium valproate can effectively inhibit the abnormal proliferation and migration of neural stem cells and newborn neurons after convulsive status epileptic

关键词： nerve regeneration status epilepticus sodium valproate long-term potentiation neural stem cells neurogenesis migration subgranular zone subventricular zone neural regeneration

Efficacy and safety of subthreshold micropulse laser in the treatment of acute central serous chorioretinopathy

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International Journal of Ophthalmology(English edition) 2023年第6期16卷 921-927页

作者： Jian-Jie Guo xiu-Juan Li Jing-Jing Wan Department of Ophthalmology the First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450000Henan ProvinceChina

·AIM:To analyze the efficacy and safety of subthreshold micropulse laser(SML)in the treatment of acute central serous chorioretinopathy(CSC).·METHODS:This is a retrospective case analysis study.Totally 58 eyes of 58 patients were enrolled,and they were divided into different groups.And 39 patients were treated with SML(SML group)and 19 patients were only observed(observation group).The follow-up period was 3mo after diagnosis.The best corrected visual acuity(BCVA),central retinal thickness(CRT),superficial retinal vascular density(SRVD),deep retinal vascular density(DRVD),the superficial and deep foveal avascular zone(FAZ)area,retinal light sensitivity(RLS),perfusion area of choroidal capillary layer(CCL),subfoveal choroidal thickness(SFCT)and fundus autofluorescence(FAF)were investigated.·RESULTS:The BCVA,CRT,SRVD,DRVD,the superficial and deep FAZ area,RLS,SFCT of SML group were significantly improved at 3mo(all Pline(all P>0.05).At the last follow-up,the BCVA and RLS in the SML group were better than those in the observation group,and CRT was lower,SRVD and DRVD,perfusion area of CCL were larger(all P<0.05).On FAF,no change of treatment spots was found after treatment.No structural laser damage was observed on optical coherence tomography(OCT)and optical coherence tomography angiography(OCTA),and no choroidal neovascularization was observed.·CONCLUSION:SML treatment of acute CSC can improve BCVA,RLS,and perfusion area of CCL,reduce CRT,increase SRVD and DRVD,and is safe.

关键词： central serous chorioretinopathy subthreshold micropulse laser optical coherence tomography angiography microperimetry

Comparison of scleral buckling using wide-angle viewing systems and indirect ophthalmoscope for rhegmatogenous retinal detachment

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International Journal of Ophthalmology(English edition) 2016年第9期9卷 1310-1314页

作者： xiu-Juan Li Xiao-Peng Yang Xiao-Bei Lyu Department of Ophthalmology the First Affiliated Hospital of Zhengzhou University Department of Medical Equipment the First Affiliated Hospital of Zhengzhou University

AIM：To compare the effects of scleral buckling using wide-angle viewing systems（WAVS） with that using indirect ophthalmoscope for the treatment of rhegmatogenous retinal detachment.METHODS：The study was a retrospective analyses of the medical records of 94 eyes（94 patients） with rhegmatogenous retinal detachment.Among them,47 eyes underwent scleral buckling using WAVS with endoiiluminator（Group W）,and 47 eyes underwent scleral buckling using indirect ophthalmoscope（Group I）.Surgical durations,primary success rate,best-corrected visual acuities（BCVA）,delayed subretinal fluid absorptions and surgical complications were compared between the two groups.RESULTS：At baseline,there were no statistical differences between the two groups in patient＇s age（P=0.997）,gender（P=0.853）,symptom duration（P=0.216）,BCVA（P=0.389）,refractive error（P=0.167）,intraocular pressure（P=0.595）,the number of retinal breaks（P=0.832）,the extent of retinal detachment（P =0.246）,subretinal demarcation line（P=0.801）,and macular detachment（P=0.811）.The follow-up period was 12 mo.The surgical durations in Group W（with or without encircling buckling） were significant shorter than those in Group I（P〈0.001 respectively）.The primary success rate was94.27%in Group W,which was similar to that in Group I（92.38%,P=0.931）.The BCVA in Group W was better than that in Group I（P〈0.001） at 1-month follow-up visit.However,there were no significant differences between the two groups at 3-month（P=0.221）,6-month（P =0.674）,and 12-month（P=0.363） follow-up visits respectively.Delayed subretinal fluid absorptions were more common in Group I than in Group W at 1-month（P=0.045） follow-up visit,but there were no significant differences between the two groups at 3-month（P=0.111）,6-month（P =1.000） and 12-month follow-up visits respectively.CONCLUSION：Scleral buckling using WAVS can be an alternative choose for rhegmatogenous retinal detachment

关键词： scleral buckling retinal detachment wide-angle viewing systems

Flanibizumab Plus Combined Surgery for Treatment o Neovascular Glaucoma with Vitreous Hemorrhage

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Chinese Medical Journal 2015年第15期128卷 2078-2083页

作者： xiu-Juan Li Xiao-Peng Yang Qiu-Ming li Yu-Ying Wang Xiao-Bei Lyu Department of Ophthalmology The First Affiliated Hospital of Zhengzhou University Zhengzhou Henan 450052 China Department of Medical Equipment The First Affiliated Hospital of Zhengzhou University Zhengzhou Henan 450052 China

Background：Neovascular glaucoma （NVG） is a refractory glaucoma.The management of NVG is very difficult,and it is more difficult when combined with vitreous hemorrhage.The aim of this study was to investigate the effects of ranibizumab plus combined surgery for NVG with vitreous hemorrhage.Methods：A total of 26 eyes of 26 NVG patients with vitreous hemorrhage were recruited in this study.The patients aged from 36 to 63 years with a mean age of 51.97 ± 7.60 years.The mean intraocular pressure （IOP） was 46.38 ± 5.75 mmHg （1 mmHg =0.133 kPa） while being treated with the maximum medical therapy.The mean best-corrected visual acuities converted to logarithm of the minimum angle of resolution （logMAR BCVA） was 2.62 ± 0.43.All the patients underwent intravitreal injection of 0.5 mg （0.05 ml） ranibizumab combined with pars plana vitrectomy （PPV）,pars plana lensectomy （PPL） with a preserved anterior capsule,panretinal photocoagulation （PRP）,and trabeculectomy （intravitreal ranibizumab [IVR] ＋ PPV ＋ PPL ＋ PRP ＋ trabeculectomy）.The IOP and logMAR BCVA were the main outcome measures in this study.Results：The follow-up period was 12 months.The mean postoperative IOPs were 26.38 ± 3.75 mmHg,21.36 ± 3.32 mmHg,1 8.57 ± 3.21 mmHg,and 16.68 ± 2.96 mmHg,respectively at 7 days,1 month,3 months,and 12 months after PPV ＋ PPL ＋ PRP ＋ trabeculectomy.At the last follow-up,the mean IOP was significantly lower than the preoperative one （t =6.612,P =0.001）.At 7 days,1 month,3 months,and 12 months after PPV ＋ PPL ＋ PRP ＋ trabeculectomy,the mean logMAR BCVA were 1.30 ± 0.36,1.29 ± 0.37,1.29 ± 0.39,and 1.26 ± 0.29,respectively.At the last follow-up,the mean logMAR BCVA was significantly improved,and the difference was statistically significant compared with preoperative one （t =6.133,P =0.002）.The logMAR BCVA improved in 22 eyes （84.62%）,and remained stable in 4 eyes （15.38%）.The neovascularization in the iris and the angle regressed significantly in all patients 7 days after ranibizumab injection.No serious complications occurred during 12 mon

关键词： Neovascular Glaucoma Panretinal Photocoagulation Pars Plana Vitrectomy Ranibizumab Trabeculectomy

Micro-vesicles from mesenchymal stem cells over-expressing miR-34a inhibit transforming growth factor-β1-induced epithelial-mesenchymal transition in renal tubular epithelial cells in vitro

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Chinese Medical Journal 2020年第7期133卷 800-807页

作者： juan He Ya-li Jiang Yan Wang xiu-juan Tian Shi-Ren Sun Department of Nephrology Xijing HospitalThe Fourth Military Medical UniversityXi’anShaanxi 710032China

Background:The use of microRNAs in the therapy of kidney disease is hampered by the difficulties in their effective delivery.Microvesicles(MVs)are known as natural carriers of small RNAs.Our prior research has demonstrated that MVs isolated from mesenchymal stem cells(MSCs)are capable of attenuating kidney injuries induced by unilateral ureteral obstruction and 5/6 sub-total nephrectomy in mice.The present study aimed to evaluate the effects of miR-34a-5p(miR-34a)-modified MSC-MVs on transforming growth factor(TGF)-β1-induced fibrosis and apoptosis in vitro.Methods:Bone marrow MSCs were modified by lentiviruses over-expressing miR-34a,from which MVs were collected for the treatment of human Kidney-2(HK-2)renal tubular cells exposed to TGF-β1(6 ng/mL).The survival of HK-2 cells was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT)and Annexin V-light 650/propidium iodide(PI)assays.The expression levels of epithelial markers(tight junction protein 1[TJP1]and E-cadherin)and mesenchymal markers(smooth muscle actin alpha(α-SMA)and fibronectin)in HK-2 cells were measured using Western blot analysis and an immunofluorescence assay.In addition,changes in Notch-1/Jagged-1 signaling were analyzed using Western blotting.Data were analyzed using a Student’s t test or one-way analysis of variance.Results:MiR-34a expression increased three-fold in MVs generated by miR-34a-modified MSCs compared with that expressed in control MVs(Plial-mesenchymal transition(EMT)markers were stronger than control MSC-MVs.The effects of miR-34a-enriched MSC-MVs on these EMT markers were str

关键词： Micro-vesicles Mesenchymal stem cells miR-34a Epithelial-mesenchymal transition

ntravitreal Ranibizumab for Aggressive Posterior Retinopathy of Prematurity

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Chinese Medical Journal 2016年第23期129卷 2879-2881页

作者： xiu-Juan Li Xiao-Peng Yang Shuang Sun Xiao-Bei Lyu Heng Jia Department of Ophthalmology The First Affiliated Hospital of Zhengzhou University Zhengzhou Henan 450052 China Department of Medical Equipment The First Affiliated Hospital of Zhengzhou University Zhengzhou Henan 450052 China Department of Ophthalmology Children's Hospital of Zhengzhou Zhengzhou Henan 450052 China

Retinopathy ofprematurity （ROP） is a proliferative disease that affects infants of young gestational age （GA） and low birth weight （BW）. Aggressive posterior ROP （AP-ROP） is a rapidly progressing and severe presentation of ROP. It is characterized as posterior location （zone 1 or posterior zone I1）, Stage 3, and with plus disease （arterial tortuosity and venous dilation）. It can quickly lead to retinal detachment and blindness if not treated in time.

关键词： Aggressive Posterior Retinopathy of Prematurity Intravitreal Injection Ranibizumab

Huangqi Decoction,a compound Chinese herbal medicine,inhibits the proliferation and activation of hepatic stellate cells by regulating the long noncoding RNA-C18orf26-1/microRNA-663a/transforming growth factor-βaxis

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Journal of Integrative Medicine 2023年第1期21卷 47-61页

作者： Ben-sheng Dong Fu-qun liu Wen-na Yang Xiao-dong li Miao-juan Shi Mao-rong li xiu-li Yan Hui Zhang Traditional Chinese Medicine Epigenomics Research Center Institute of Interdisciplinary Integrative Medicine ResearchShanghai University of Traditional Chinese MedicineShanghai 201203China Department of Rheumatology and Immunology Nanjing Lishui District Hospital of Traditional Chinese MedicineNanjing 211299Jiangsu ProvinceChina Department of Rheumatology and Immunology Yangzhou University Medical CollegeYangzhou 225000Jiangsu ProvinceChina Department of Cardiovascular Physiology Faculty of MedicineKagawa UniversityKita-gunKagawa 761-0793Japan Department of Gastroenterology Yueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghai 200437China

Objective Huangqi Decoction(HQD),a classical traditional Chinese medicine formula,has been used as a valid treatment for alleviating liver fibrosis;however,the underlying molecular mechanism is still unknown.Although our previous studies showed that microRNA-663a(miR-663a)suppresses the proliferation and activation of hepatic stellate cells(HSCs)and the transforming growth factor-β/small mothers against decapentaplegic(TGF-β/Smad)pathway,whether long noncoding RNAs(lncRNAs)are involved in HSC activation via the miR-663a/TGF-β/Smad signaling pathway has not yet reported.The present study aimed to investigate the roles of lncRNA lnc-C18orf26-1 in the activation of HSCs and the mechanism by which HQD inhibits hepatic fibrosis.Methods The expression levels of lnc-C18orf26-1,miR-663a and related genes were measured by quantitative reverse transcription-polymerase chain reaction.HSCs were transfected with the miR-663a mimic or inhibitor and lnc-C18orf26-1 small interfering RNAs.The water-soluble tetrazolium salt-1 assay was used to assess the proliferation rate of HSCs.Changes in lncRNA expression were evaluated in miR-663a-overexpressing HSCs by using microarray to identify miR-663a-regulated lncRNAs.RNA hybrid was used to predict the potential miR-663a binding sites on lncRNAs.Luciferase reporter assays further confirmed the interaction between miR-663a and the lncRNA.The expression levels of collagen α-2(I)chain(COL1A2),α-smooth muscle actin(α-SMA)and TGF-β/Smad signaling pathway-related proteins were determined using Western blotting.Results Lnc-C18orf26-1 was upregulated in TGF-β1-activated HSCs and competitively bound to miR-663a.Knockdown of lnc-C18orf26-1 inhibited HSC proliferation and activation,downregulated TGF-β1-stimulatedα-SMA and COL1A2 expression,and inhibited the TGF-β1/Smad signaling pathway.HQD suppressed the proliferation and activation of HSCs.HQD increased miR-663a expression and decreased lnc-C18orf26-1 expression in HSCs.Further studies showed that HQD inhibited the expression of COL1A2,α-SMA,T

关键词： Longnoncoding RNA-C18orf26-1 MicroRNA-663a Transforming growth factor-β Hepatic stellate cells Huangqi Decoction

Intravitreal bevacizumab injection for chronic central serous chorioretinopathy

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Chinese Medical Journal 2010年第15期123卷 2145-2147页

作者： li xiu-juan ZHANG Jin-song Department of Ophthalmology the First Affiliated Hospital of Zhengzhou University Zhengzhou Henan 450052 China

Central serous chorioretinopathy （CSC） is characterized by an idiopathic serous neurosensory detachment primarily affecting the macula.In most cases,the disorder is self-limited and spontaneously in 4 to 6 months, a... 详细信息

关键词： central serous chorioretinopathy intravitreal bevacizumab retinal pigment epithelium detachment

Impact of the COVID-19 pandemic on endoscopic procedures:a single-center study in China

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World Journal of Emergency Medicine 2022年第4期13卷 301-304页

作者： Lai-juan Chen xiu-jing Yu Ting Chen Mei-feng Wu Yong-li Ye Lun-po Wu Nursing Department the Second Affiliated Hospital of Zhejiang University School of MedicineHangzhou 310009China Department of Endoscopy Center the Second Affiliated Hospital of Zhejiang University School of MedicineHangzhou 310009China Department of Gastroenterology the Second Affiliated Hospital of Zhejiang University School of MedicineHangzhou 310009China Institution of Gastroenterology Zhejiang UniversityHangzhou 310009China

The coronavirus disease 2019(COVID-19)has affected the whole globe since its outbreak in December 2019,[1]and many countries have tried several measures to protect and mitigate against its spread.Although the outbreaks have been effectively controlled in China,COVID-19 is still emerging as a public health crisis worldwide,causing massive panic.[2]Additionally,there are still scattered new cases being reported in China.[3]Therefore,in order to adhere to the policy of preventing imported cases and domestic resurgence,varied strategic countermeasures against COVID-19 have been enforced on a regular basis.As respiratory droplet and contact are the main modes of COVID-19 transmission,[4]all endoscopy centers have a higher risk of exposure to COVID-19 than other areas in a hospital.

关键词： Impact scattered policy