检索结果-南通市图书馆

Fine mapping of a major QTL qHYF_B06 for peanut yield

在线全文

学校读者我要写书评

暂无评论

作物学报:英文版 2023年第5期11卷 1533-1540页

作者： Yongqing Yang Qiao Su Yurong Li Zengshu Cheng Yahui Song xinxin jin jin Wang Institute of Cereal and Oil Crops Hebei Academy of Agricultural and Forestry Sciencesthe Key Laboratory of Crop Genetics and Breeding of HebeiShijiazhuang 050035HebeiChina

High yield is a major objective for peanut(Arachis hypogaea L.) breeding worldwide. However, fewer yield-related quantitative trait loci(QTL) have been reported in peanut than in other staple food crops such as rice(Oryza sativa), wheat(Triticum aestivum), and maize(Zea mays). This study aimed to identify stable major-effect QTL associated with pod yield per plant, hundred-pod weight for double-seeded pods,hundred-seed weight, shelling percentage, and pod number per plant, allowing us to predict candidate genes by means of transcriptome and genome sequencing. To this end, we used a population of recombinant inbred lines comprising 192 F9:11families derived from a JH6 × KX01-6 cross to construct a highresolution genetic map(1705.7 c M) consisting of 2273 polymorphic SNPs, with 0.75 c M(on average)between adjacent SNPs. We identified two high-confidence, yield-related QTL, qHYF_A08 and qHYF_B06, explaining 5.78%–31.40% of phenotypic variation and with LOD values of 5.10–24.48, in six environments. qHYF_A08 mainly explained the variation in shelling percentage, whereas qHYF_B06explained variation in hundred-pod weight and hundred-seed weight and accounted for 8.77%–31.40%of the variation in effective pod number per plant, pod number per plant, and shelling percentage. We narrowed down qHYF_B06 to an 890-kb interval using an advanced mapping population.Transcriptome and genome analyses revealed that only Arahy.129FS0 and Arahy.3R9A5K in the candidate mapping interval were differentially expressed between JH6 and KX01-6, with substantial structural variations in their promoter and coding regions. Genotypes of 208 peanut accessions determined using a diagnostic CAPS marker suggested that the two haplotypes of Arahy.3R9A5K were highly associated with hundred-seed weight and hundred-pod weight;this diagnostic CAPs marker could therefore be useful for selecting high-yielding lines during peanut breeding. Overall, our results provide valuable information for cloning alleles with favorable effects on peanut yield.

关键词： Arachis hypogaea Candidate gene E3 ubiquitin ligase Yield-related QTL region

Osteoimmunology research in rheumatoid arthritis:From single-cell omics approach

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2023年第14期136卷 1642-1652页

作者： Nan Hu jing Wang Bomiao Ju Yuanyuan Li Ping Fan xinxin jin Xiaomin Kang Shufang Wu Department of Rheumatology The First Affiliated Hospital of Xi’an Jiaotong UniversityXi’anShaanxi 710061China Department of Physiology and Pathophysiology School of Basic Medical SciencesXi’an Jiaotong University Health Science CenterXi’anShaanxi 710061China Center for Translational Medicine The First Affiliated Hospital of Xi’an Jiaotong UniversityXi’anShaanxi 710061China

Cellular immune responses as well as generalized and periarticular bone loss are the key pathogenic features of rheumatoid arthritis(RA).Under the pathological conditions of RA,dysregulated inflammation and immune processes tightly interact with skeletal system,resulting in pathological bone damage via inhibition of bone formation or induction of bone resorption.Singlecell omics technologies are revolutionary tools in the field of modern biological research.They enable the display of the state and function of cells in various environments from a single-cell resolution,thus making it conducive to identify the dysregulated molecular mechanisms of bone destruction in RA as well as the discovery of potential therapeutic targets and biomarkers.Here,we summarize the latest findings of single-cell omics technologies in osteoimmunology research in RA.These results suggest that single-cell omics have made significant contributions to transcriptomics and dynamics of specific cells involved in bone remodeling,providing a new direction for our understanding of cellular heterogeneity in the study of osteoimmunology in RA.

关键词： Rheumatoid arthritis Osteoimmunology Single-cell omics Single-cell RNA sequencing scATAC-seq

An Auto-Grading Oriented Approach for Off-Line Handwritten Organic Cyclic Compound Structure Formulas Recognition

在线全文

学校读者我要写书评

暂无评论

Computer Modeling in Engineering & Sciences 2023年第6期135卷 2267-2285页

作者： Ting Zhang Yifei Wang xinxin jin Zhiwen Gu Xiaoliang Zhang Bin He Faculty of Artificial Intelligence in Education Central China Normal UniversityWuhan430079China

Auto-grading,as an instruction tool,could reduce teachers’workload,provide students with instant feedback and support highly personalized learning.Therefore,this topic attracts considerable attentions from researchers recently.To realize the automatic grading of handwritten chemistry assignments,the problem of chemical notations recognition should be solved first.The recent handwritten chemical notations recognition solutions belonging to the end-to-end trainable category suffered fromthe problem of lacking the accurate alignment information between the input and output.They serve the aim of reading notations into electrical devices to better prepare relevant edocuments instead of auto-grading handwritten assignments.To tackle this limitation to enable the auto-grading of handwritten chemistry assignments at a fine-grained level.In this work,we propose a component-detectionbased approach for recognizing off-line handwritten Organic Cyclic Compound Structure Formulas(OCCSFs).Specifically,we define different components of OCCSFs as objects(including graphical objects and text objects),and adopt the deep learning detector to detect them.Then,regarding the detected text objects,we introduce an improved attention-based encoder-decoder model for text recognition.Finally,with these detection results and the geometric relationships of detected objects,this article designs a holistic algorithm for interpreting the spatial structure of handwritten OCCSFs.The proposedmethod is evaluated on a self-collected data set consisting of 3000 samples and achieves promising results.

关键词： Handwritten chemical structure formulas structure interpretation components detection text recognition

Super-enhancer receives signals from the extracellular matrix to induce PD-L1-mediated immune evasion via integrin/ BRAF/TAK1/ERK/ETV4 signaling

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cancer Biology & Medicine 2022年第5期19卷 669-684页

作者： Panpan Ma xinxin jin Zhiwei Fan Zhou Wang Suhui Yue Changyue Wu Shiyin Chen Yuanyuan Wu Miaomiao Chen Donghua Gu Siliang Zhang Renfang Mao Yihui Fan Laboratory of Medical Science School of MedicineNantong UniversityNantong 226001China Department of Pathogenic Biology School of MedicineNantong UniversityNantong 226001China Department of Clinical Laboratory Yancheng No.1 People’s HospitalYancheng 224005China School of Life Sciences Nantong UniversityNantong 226001China Department of Dermatology Affiliated Hospital of Nantong UniversityNantong UniversityNantong 226001China The Department of Urology the Second Affiliated Hospital of Nantong UniversityNantong UniversityNantong 226001China The Department of Radiotherapy Oncology Harbin Medical University Cancer HospitalHarbin 150086China Department of Pathophysiology School of MedicineNantong UniversityNantong 226001China

Objective:PD-L1 and PD-L2 expression levels determine immune evasion and the therapeutic efficacy of immune checkpoint blockade.The factors that drive inducible PD-L1 expression have been extensively studied,but mechanisms that result in constitutive PD-L1 expression in cancer cells are largely unknown.Methods:DNA elements were deleted in cells by CRISPR/Cas9-mediated knockout.Protein function was inhibited by chemical inhibitors.Protein levels were examined by Western blot,mRNA levels were examined by real-time RT-PCR,and surface protein expression was determined by cellular immunofluorescence and flow cytometry.Immune evasion was examined by in vitro T cell-mediated killing.Results:We determined the core regions(chr9:5,496,378–5,499,663)of a previously identified PD-L1L2-super-enhancer(SE).Through systematic analysis,we found that the E26 transformation-specific(ETS)variant transcription factor(ETV4)bound to this core DNA region but not to DNA surrounding PD-L1L2SE.Genetic knockout of ETV4 dramatically reduced the expressions of both PD-L1 and PD-L2.ETV4 transcription was dependent on ERK activation,and BRAF/TAK1-induced ERK activation was dependent on extracellular signaling fromαvβ3 integrin,which profoundly affected ETV4 transcription and PD-L1/L2 expression.Genetic silencing or pharmacological inhibition of components of the PD-L1L2-SE-associated pathway rendered cancer cells susceptible to T cell-mediated killing.Conclusions:We identified a pathway originating from the extracellular matrix that signaled via integrin/BRAF/TAK1/ERK/ETV4 to PD-L1L2-SE to induce PD-L1-mediated immune evasion.These results provided new insights into PD-L1L2-SE activation and pathways associated with immune checkpoint regulation in cancer.

关键词： PD-L1 super-enhancer ETV4 ERK αvβ3 integrin BRAF TAK1

Additive manufacturing of hydroxyapatite bioceramic scaffolds: Dispersion, digital light processing, sintering, mechanical properties, and biocompatibility

在线全文

学校读者我要写书评

暂无评论

Journal of Advanced Ceramics 2020年第3期9卷 360-373页

作者： Chengwei FENG Keqiang ZHANG Rujie HE Guojiao DING Min XIA xinxin jin Chen XIE Instiute of Advanced Structure Technology Bejing Institute of TechnologyBeijing 100081China School of Materials Science and Engineering Beijing Institute of TechnologyBejing 100081China School of Materials Science and Engineering Harbin University of Science and TechnologyHarbin 150040China Shanghai Aircrafi Manufacturing Co. LtdShanghai 201324China

Hydroxyapatite(HA)bioceramic scaffolds were fabricated by using digital light processing(DLP)based additive manufacturing.Key issues on the HA bioceramic scaffolds,including dispersion,DLP fabrication,sintering,mechanical properties,and biocompatibility were discussed in detail.Firstly,the ffects of dispersant dosage,solid loading,and sintering temperature were studied.The optimal dispersant dosage,solid loading,and sintering temperature were 2wt%,50vol%,and 1250℃,respectively.Then,the mechanical properties and biocompatibility of the HA bioceramic scaffolds were investigated.The DLP-prepared porous HA bioceramic scaffold was found to exhibit excellent mechanical properties and degradation behavior.From this study,DLP technique shows good potential for manufacturing HA bioceramic scaffolds.

关键词： additive manufacturing digital light processing vat photopolymerization hydroxyapatite bioceramic scaffold

在线全文

学校读者我要写书评

暂无评论

关于《蒙古字韵》第一版的若干史实考述

西部蒙古论坛 2024年第2期 52-61+127页

作者：金欣欣人民教育出版社课程教材研究所

八思巴字是元代国师八思巴在元代建立前主持创制的一种拼音文字。《蒙古字韵》第一版则是在元世祖忽必烈时期编纂成的一部用八思巴字标注汉字读音的韵书。《蒙古字韵》反映了13世纪中后期元代汉语共同语的语音情况，是汉语音韵学中关于... 详细信息

八思巴字是元代国师八思巴在元代建立前主持创制的一种拼音文字。《蒙古字韵》第一版则是在元世祖忽必烈时期编纂成的一部用八思巴字标注汉字读音的韵书。《蒙古字韵》反映了13世纪中后期元代汉语共同语的语音情况，是汉语音韵学中关于元代汉语的一部重要文献资料，具有较高的语音史研究价值。此外，它对于少数民族文字与汉文双语辞书编纂研究、汉语韵书的发展史、文物考证、元代文化教育的研究等，也都有重要意义。关于《蒙古字韵》第一版的作者、成书时间和主要读者对象，学术界有不同观点。本文对此做了进一步探讨。

关键词：《蒙古字韵》第一版八思巴字考述文化交流

Spatial-temporal variations and driving factors of soil organic carbon in forest ecosystems of Northeast China

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Forest Ecosystems 2023年第2期10卷 141-152页

作者： Shuai Wang Bol Roland Kabindra Adhikari Qianlai Zhuang xinxin jin Chunlan Han Fengkui Qian College of Land and Environment Shenyang Agricultural UniversityShenyang110866China Key Laboratory of Ecosystem Network Observation and Modeling Institute of Geographic Sciences and Natural Resources ResearchCASBeijing100101China Institute of Bio-and Geosciences Agrosphere(IBG-3)Forschungszentrum Jülich GmbHWilhelm-Johnen-Straße52428JülichGermany School of Natural Sciences Environment Centre WalesBangor UniversityBangorLL572UWUK USDA-ARS GrasslandSoil and Water Research LaboratoryTempleTX76502USA Department of Earth Atmosphericand Planetary SciencesPurdue UniversityWest LafayetteIN47907USA

Forest soil carbon is a major carbon pool of terrestrial ecosystems,and accurate estimation of soil organic carbon(SOC)stocks in forest ecosystems is rather challenging.This study compared the prediction performance of three empirical model approaches namely,regression kriging(RK),multiple stepwise regression(MSR),random forest(RF),and boosted regression trees(BRT)to predict SOC stocks in Northeast China for 1990 and 2015.Furthermore,the spatial variation of SOC stocks and the main controlling environmental factors during the past 25 years were identified.A total of 82(in 1990)and 157(in 2015)topsoil(0–20 cm)samples with 12 environmental factors(soil property,climate,topography and biology)were selected for model construction.Randomly selected80%of the soil sample data were used to train the models and the other 20%data for model verification using mean absolute error,root mean square error,coefficient of determination and Lin's consistency correlation coefficient indices.We found BRT model as the best prediction model and it could explain 67%and 60%spatial variation of SOC stocks,in 1990,and 2015,respectively.Predicted maps of all models in both periods showed similar spatial distribution characteristics,with the lower SOC in northeast and higher SOC in southwest.Mean annual temperature and elevation were the key environmental factors influencing the spatial variation of SOC stock in both periods.SOC stocks were mainly stored under Cambosols,Gleyosols and Isohumosols,accounting for 95.6%(1990)and 95.9%(2015).Overall,SOC stocks increased by 471 Tg C during the past 25 years.Our study found that the BRT model employing common environmental factors was the most robust method for forest topsoil SOC stocks inventories.The spatial resolution of BRT model enabled us to pinpoint in which areas of Northeast China that new forest tree planting would be most effective for enhancing forest C stocks.Overall,our approach is likely to be useful in forestry management and ecological restoration at and beyond the regional scale.

关键词： Soil organic carbon stocks Forest ecosystem Spatial-temporal variation Carbon sink Digital soil mapping

FeCo-based mesoporous carbon shells modified N-doped porous carbon spheres for oxygen reduction reaction

在线全文

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2021年第4期31卷 527-535页

作者： xinxin jin Yan Xie Chaoyue Zhao Yinghao Xu Yang Lv He Wang Li Chen Jiahui Huang Dalian National Laboratory for Clean Energy Dalian Institute of Chemical Physics Chinese Academy of Sciences University of Chinese Academy of Sciences Dalian University of Technology Shanghai Key Laboratory of Green Chemistry and Chemical Processes School of Chemistry and Molecular Engineering East China Normal University

Fe Co-based non-noble metal electrocatalysts(NNMEs) of Fe Co/MCS-NPCS was fabricated by immobilization of hemin on mesoporous carbon shells modified N-doped porous carbon spheres(MCS-NPCS). The obtained Fe Co/MCS-NPCS exhibits a half-wave potential(E1/2) of 0.851 V versus the reversible hydrogen electrode(vs. RHE) and a limited-diffusion current density(JL) of 5.45 m A cm-2. In addition, Fe Co/MCS-NPCS shows comparable oxygen reduction reaction(ORR) performances to 20 wt% Pt/C in terms of E1/2and JLand better electrochemical properties, including the methanol tolerance and durability in alkaline solution. Such outstanding electrochemical activities of Fe Co/MCS-NPCS can be ascribed to Fe and/or Co-based nitrides and carbides as well as N-doped carbon matrixes modified with mesoporous carbon shells. This research introduces a promising path to design and synthesize highly efficient Fe Co–N–C electrocatalysts towards ORR.

关键词： Zeolitic imidazolate framework Mesoporous carbon shell N-doped porous carbon Metal-macrocycle Oxygen reduction reaction

基于RTP晶体的271 cm^(−1)拉曼频移五阶斯托克斯激光

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

强激光与粒子束 2024年第6期36卷 54-58页

作者：王京金鑫鑫王严毅王鸿雁李志红段延敏朱海永温州大学电气与电子工程学院温州市微纳光电器件重点实验室浙江温州325035 青岛海泰光电技术有限公司山东青岛266100

报道了端面泵浦被动调Q激光驱动RbTiOPO4晶体拉曼实现271 cm^(−1)频移高阶斯托克斯激光输出。采用Nd:YAG与Cr4+:YAG键合设计来降低腔内的损耗,并使激光系统更紧凑,从而提升腔内光子功率密度,有利于拉曼频移向高阶斯托克斯激光转换。设... 详细信息

报道了端面泵浦被动调Q激光驱动RbTiOPO4晶体拉曼实现271 cm^(−1)频移高阶斯托克斯激光输出。采用Nd:YAG与Cr4+:YAG键合设计来降低腔内的损耗,并使激光系统更紧凑,从而提升腔内光子功率密度,有利于拉曼频移向高阶斯托克斯激光转换。设计不同频移的一阶斯托克斯激光对应在不同腔内振荡,利用与基频光模式匹配的差异来抑制687 cm^(−1)频移的一阶斯托克斯激光,最终获得较纯的271 cm^(−1)频移的五阶斯托克斯激光输出。在泵浦功率8.1 W下,获得了平均输出功率230 mW的1244 nm波长激光,对应的脉冲宽度和重复频率分别为2.9 ns和11.7 kHz。1244 nm波段的激光正好与水中OH−1吸收峰对应,在地表植被和行星水含量的检测等领域有重要的应用。

关键词： RTP晶体拉曼激光被动调Q 五阶斯托克斯光