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Science China(Life Sciences) 2023年第12期66卷 2910-2921页

作者： Jianduo Zhang Lu Zhang Chengwei Zhang Yongxing Yang Huayin liu Lu Li Shengxue Zhang Xianggan Li xinxiang liu Ya liu Jin Wang Guangyu Yang Qingyou Xia Weiguang Wang Jinxiao Yang Yunnan Key Laboratory of Tobacco Yunnan Academy of Tobacco ScienceKunming 650231China Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding Beijing Academy of Agriculture&Forestry SciencesBeijing 100089China Key Laboratory of Natural Products Synthetic Biology of Ethnic Medicinal Endophytes State Ethnic Affairs CommissionYunnan Minzu UniversityKunming 650031China Cropedit Biotechnology Co.Ltd. Beijing 102206China Biological Science Research Center Southwest UniversityChongqing 400716China

Prime editing(PE)is a versatile CRISPR-Cas based precise genome-editing platform widely used to introduce a range of possible base conversions in various ***,no PE systems have been shown to induce heritable mutations in tobacco,nor in any other *** this study,we generated an efficient PE system in tobacco that not only introduced heritable mutations,but also enabled anthocyanin-based reporter selection of transgene-free T_(1) *** system was used to confer Zabienol biosynthesis in the allotetraploid tobacco cultivar HHDJY by restoring a G>T conversion in the NtCPS2 *** levels of Z-abienol were detected in the leaves of homozygous T_(1) plants at two weeks after *** study describes an advance in PE systems and expands genome-editing toolbox in tobacco,even in dicots,for use in basic research and molecular *** restoring biosynthesis of Z-abienol in tobacco might provide an efficient way to obtain Z-abienol in plants.

关键词： prime editing Z-abienol biosynthesis visible marker CRISPR Cas9 tobacco

Using Riccati Equation to Construct New Solitary Solutions of Nonlinear Difference Differential Equations

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American Journal of Computational Mathematics 2022年第2期12卷 256-266页

作者： xinxiang liu Kaiwen Cui Guojiang Wu Hefei Thomas School Hefei China The Second High School Attached to Beijing Normal University International Department Beijing China Institute of Plasma Physics Hefei Institutes of Physical Science Chinese Academy of Sciences Hefei China

In this paper, we use Riccati equation to construct new solitary wave solutions of the nonlinear evolution equations (NLEEs). Through the new function transformation, the Riccati equation is solved, and many new solitary wave solutions are obtained. Then it is substituted into the (2 + 1)-dimensional BLMP equation and (2 + 1)-dimensional KDV equation as an auxiliary equation. Many types of solitary wave solutions are obtained by choosing different coefficient p1 and q1 in the Riccati equation, and some of them have not been found in other documents. These solutions that we obtained in this paper will be helpful to understand the physics of the NLEEs.

关键词： Nonlinear Evolution Equations Hyperbolic Function Riccati Equation Auxiliary Equation Solitary Wave Solutions

Potassium alleviated high nitrogen-induced apple growth inhibition by regulating photosynthetic nitrogen allocation and enhancing nitrogen utilization capacity

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Horticultural Plant Journal 2024年第1期10卷 1-14页

作者： xinxiang Xu Guangyuan liu Jingquan liu Mengxue Lyu Fen Wang Yue Xing Hao Meng Min Li Yu Jiang Ge Tian Zhanling Zhu Yuanmao Jiang Shunfeng Ge State Key Laboratory of Crop Biology College of Horticulture Science and EngineeringShandong Agricultural UniversityTai'anShandong 271018China Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong College of Biological and Agricultural EngineeringWeifang UniversityWeifangShandong 261061China

There is a close relationship between potassium(K)and nitrogen(N).However,the roles of K under high N conditions remain *** a hydroponics approach,we monitored the morphological,physiological,and molecular changes in M9T337 apple(Malus domestica)rootstocks under different nitrate(10 and 30 mmol·L^(-1)NO_(3)^(-))and K supply(0.5,6,10,and 20 mmol·L_(-1)K^(+))*** revealed that high nitrate inhibited the root growth of M9T337 rootstocks,downregulated the expressions of K transporter genes(MdPT5,MdHKT1,and MdATK1),and reduced the net NO3-and K+influx at the surface of roots,thereby resulting in an N/K imbalance in *** investigation showed that 10 mmol·L^(-1)K increased the activity of N metabolic enzymes(NR,GS,NiR,and GOGAT),upregulated the expressions of genes related to nitrate uptake and transport(MdNRT1.1,MdNRT1.2,MdNRT1.5,and MdNRT2.4),promoted15N transport from the roots to the shoots,optimized leaf N distribution,and improved photosynthetic N utilization efficiency under high nitrate *** results suggest that the negative effects of high nitrate may be related to the N/K imbalance and that reducing N/K in plants by increasing K supply level can effectively alleviate the inhibition of N assimilation by high nitrate stress.

关键词： Apple rootstock K level High nitrate stress N metabolism ^(15)N

3D anchoring structured for LiFe_(0.5)Mn_(0.5)PO_(4)@cornstalk-C cathode materials

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中国化学快报:英文版 2023年第8期34卷 302-305页

作者： Guangliang Zhang Riran Zang Man Mo Zhijie Fang Yangxian Huang Kunsong Hu Jiali Huang xinxiang liu Lingyun Huang Guohui Kang Weijian Li Haiqing Zhan Xianquan Ming Guanhan Huang Guiliang Li Feng Zhan School of Resources Environment and Materials Guangxi UniversityNanning 530004China School of Electronics Engineering Guangxi University of Science and TechnologyLiuzhou 545006China State Key Laboratory of Featured Metal Materials and Life-cycle Safety for Composite Structures Guangxi UniversityNanning 530004China Guangxi Higher Education Key Laboratory of High Performance Structural Materials and Heat Treatment&Surface Processing Guangxi UniversityNanning 53004China South Manganese Group Limited Nanning 530029China

The organic carbon source coating LiFe_(x)Mn_(1-x)PO_(4)suffers from the problem of non-uniform carbon *** thick carbon cladding layer instead hinders the de-embedding of lithium *** this paper,we choose cornstalk as the carbon source,then LiFe_(0.5)Mn_(0.5)PO_(4)@cornstalk-C(LFMP@C-C)with 3D anchoring structure is prepared by the solvothermal *** results show that the LFMP with cornstalk as the carbon source has better performance compared to the sucrose-coated LFMP material(LFMP@C).The discharge capacity of LFMP@C-C is 116 mAh/g for the first cycle at 1 C and the capacity retention rate is 94.0%after 500 cycles,and the discharge capacity of LFMP@C-C is more than 17.17%higher than that of LFMP@C.

关键词： LiFe_(0.5)Mn_(0.5)PO_(4) Cornstalk Solvothermal method 3D anchoring structure Cathode materials

Genetic analysis of transcription factors in dopaminergic neuronal development in Parkinson’s disease

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Chinese Medical Journal 2024年第4期137卷 450-456页

作者： Yuwen Zhao Lixia Qin Hongxu Pan Tingwei Song Yige Wang Xiaoxia Zhou Yaqin Xiang Jinchen Li Zhenhua liu Qiying Sun Jifeng Guo xinxiang Yan Beisha Tang Qian Xu Department of Neurology Xiangya HospitalCentral South UniversityChangshaHunan 410008China Department of Neurology The Second Xiangya HospitalCentral South UniversityChangshaHunan 410011China Bioinformatics Center National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangshaHunan 410008China Department of Geriatrics Xiangya HospitalCentral South UniversityChangshaHunan 410008China Centre for Medical Genetics and Hunan Key Laboratory of Medical Genetics School of Life SciencesCentral South UniversityChangshaHunan 410008China Key Laboratory of Hunan Province in Neurodegenerative Disorders Central South UniversityChangshaHunan 410008China

Background:Genetic variants of dopaminergic transcription factor-encoding genes are suggested to be Parkinson’s disease(PD)risk factors;however,no comprehensive analyses of these genes in patients with PD have been ***,we aimed to genetically analyze 16 dopaminergic transcription factor genes in Chinese patients with ***:Whole-exome sequencing(WES)was performed using a Chinese cohort comprising 1917 unrelated patients with familial or sporadic early-onset PD and 1652 ***,whole-genome sequencing(WGS)was performed using another Chinese cohort comprising 1962 unrelated patients with sporadic late-onset PD and 1279 ***:We detected 308 rare and 208 rare protein-altering variants in the WES and WGS cohorts,***-based association analyses of rare variants suggested that MSX1 is enriched in sporadic late-onset ***,the significance did not pass the Bonferroni ***,72 and 1730 common variants were found in the WES and WGS cohorts,***,single-variant logistic association analyses did not identify significant associations between common variants and ***:Variants of 16 typical dopaminergic transcription factors might not be major genetic risk factors for PD in Chinese ***,we highlight the complexity of PD and the need for extensive research elucidating its etiology.

关键词： Parkinson’s disease Transcription factors Dopaminergic neurons Genetic Variants

Al_2O_3-TiO_2复合载体用于乙炔选择加氢反应

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石油化工 2006年第5期35卷 411-415页

作者：韦以刘新香中国石油化工股份有限公司北京化工研究院北京100013

采用共沉淀法制备了A l2O3-T iO2复合载体(简称复合载体),研究了该复合载体负载Pd后制备的Pd/A l2O3-T iO2催化剂在乙炔选择加氢反应中的催化性能。考察了复合载体合成条件(复合载体中T iO2的含量、中和反应温度、沉淀终点pH、A lC l3... 详细信息

采用共沉淀法制备了A l2O3-T iO2复合载体(简称复合载体),研究了该复合载体负载Pd后制备的Pd/A l2O3-T iO2催化剂在乙炔选择加氢反应中的催化性能。考察了复合载体合成条件(复合载体中T iO2的含量、中和反应温度、沉淀终点pH、A lC l3的质量浓度、沉淀物老化时间)对Pd/A l2O3-T iO2催化剂活性和选择性的影响。实验结果表明,复合载体的最佳合成条件为:T iO2质量分数30%,中和反应温度40℃,沉淀终点pH=9,A lC l3的质量浓度0.20g/mL,沉淀物不老化。采用最佳条件下合成的复合载体所制备的Pd/A l2O3-T iO2催化剂(Cat-02)的选择性大于85%,乙炔转化率达到90%以上,优于Pd/A l2O3催化剂。与工业催化剂G-58C相比,Cat-02在活性上还有一定差距,但具有更好的选择性。

关键词：钯氧化铝氧化钛复合载体乙炔选择加氢乙烯

Al_2O_3-TiO_2复合载体的制备与表征

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石油化工 2006年第2期35卷 173-177页

作者：韦以刘新香中国石油化工股份有限公司北京化工研究院北京100013

采用共沉淀法制备了A l2O3-T iO2复合载体,采用BET法、压汞法、扫描电子显微镜、X射线衍射、吡啶吸附-程序升温脱附和吡啶吸附红外光谱法对A l2O3-T iO2复合载体进行了表征。讨论了焙烧温度对A l2O3-T iO2复合载体的比表面积、孔径分布... 详细信息

采用共沉淀法制备了A l2O3-T iO2复合载体,采用BET法、压汞法、扫描电子显微镜、X射线衍射、吡啶吸附-程序升温脱附和吡啶吸附红外光谱法对A l2O3-T iO2复合载体进行了表征。讨论了焙烧温度对A l2O3-T iO2复合载体的比表面积、孔径分布及晶型的影响,观察了A l2O3-T iO2复合载体的形貌,同时对A l2O3-T iO2复合载体的表面酸性进行了研究。实验结果发现,随焙烧温度的升高,A l2O3-T iO2复合载体的比表面积减小、孔体积和平均孔径增大;A l2O3-T iO2复合载体中T iO2的存在降低了A l2O3晶型的转变温度,而A l2O3的存在则升高了T iO2的晶型转变温度,提高了T iO2的热稳定性;A l2O3-T iO2复合载体表面具有较低的酸性,且只有L酸中心;A l2O3-T iO2复合载体为球状微粒。

关键词：共沉淀法氧化铝二氧化钛复合载体孔结构晶型催化剂

Divalent anion intercalation and etching-hydrolysis strategies to construct ultra-stable electrodes for seawater splitting

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Science China Chemistry 2024年第2期67卷 687-695页

作者： Jiajia Lu Yang liu Han-Pu Liang Key Laboratory of Biofuels Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdao266101China Henan Provincial Key Laboratory of Nanocomposites and Applications Institute of Nano-Structured Functional MaterialsHuanghe Science and Technology CollegeZhengzhou450006China School of Materials Science and Engineering Henan Normal UniversityXinxiang453007China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing100049China Shandong Energy Institute Qingdao266101China

Developing stable electrodes for seawater splitting remains a great challenge due to the detachment of catalysts at a large operating current and severe anode corrosion caused by ***,divalent anion intercalation and etching-hydrolysis strategies are deployed to synthesize the ultra-stable anode,dendritic Fe(OH)_(3) grown on Ni(SO_4)_(0.3)(OH)_(1.4)–Ni(OH)_(2).Experimental results reveal that the anode exhibits good activity and excellent stability in alkaline simulated *** 500 h,the current density operated at 1.72 V remains 99.5%,about 210 m A cm^(-2).The outstanding stability originates from the etchinghydrolysis strategy,which strengthens the interaction between the catalyst and the carrier and retards thus the detachment of catalysts at a large current ***,theoretical simulations confirm that the intercalated divalent anions,such as SO_4^(2-) and CO_(3)^(2-),can weaken the adsorption strength of chlorine on the surface of catalysts and hinder the coupling and hybridization between chlorine and nickel,which slows down the anode corrosion and improves catalytic ***,the twoelectrode system shows the remarkable 95.1% energy efficiency at 2,000 A m-2and outstanding stability in 6 mol L^(-1) KOH +seawater at 80 ℃.

关键词： seawater splitting stability divalent anion intercalation etching-hydrolysis

Search for New Antiphytovirucides Ⅷ.Phosphorylation,Sulphonation and Carboxymethylation of Benzimidazole Derivatives and Antiviral Activity of Products

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Wuhan University Journal of Natural Sciences 1998年第2期3卷 201-204页

作者： Qu Fanqi, Dong Xinrong, Luo xinxiang, liu Xin, Huang Xiaoling(College of Chemistry, Wuhan University, Wuhan 430072, China)

The Iead compound of substituted 2-(4-hydroxyphenyl)benzimidazol were modified by phos-phorylation sulfonation and carboxymethylation. The henzimldazoloamidophosphate (6) (7) have better activi-ty against tobacco mosaic virus (TMV). The benzimldazolophenoxyacetic acid, phenoxysuIfonic acid c0ntaining tri flu0r0methyl, and nitro gr0up have 77% ~85% efflclency f0r wheat rust disease.

关键词： 2-(4'-hydroxyphenyl) benzimidazole phosphorylation sulfonation carboxymethylation an-tiphytovirucide

Effects of pyraclostrobin on growth,oxidative stress,and gene expression in relation to stress and ATP-binding cassette transporters in Tetrahymena thermophila

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Journal of Oceanology and Limnology 2024年第1期42卷 150-162页

作者： Yang liu Jiale ZHANG Peng XIAO Xin liu Yisifu MA Jing ZHANG Bangjun ZHANG Henan International Joint Laboratory of Aquatic Ecotoxicology and Health Protection Xinxiang 453007China College of Life Sciences Henan Normal UniversityXinxiang 453007China Journal of Henan Normal University Xinxiang 453007China College of Life and Environmental Science Wenzhou UniversityWenzhou 325035China Department of Ecosystem Studies School of Environmental ScienceUniversity of Shiga PrefectureShiga 522-8533Japan

Pyraclostrobin(PYR),a widely used fungicide,has negative effects on fish and algae,but its toxicity in protozoa remains *** this study,the effects of PYR on the growth,oxidative stress,and gene expression related to stress and ATP-binding cassette(ABC)transporters in Tetrahymena thermophila were *** result showed that the 96-h IC_(50)of PYR against *** was 17.2 mg/***,PYR inhibited the growth of *** in concentration-or time-dependent manner.A morphological study revealed that the shape and size of *** changed,and damage of cell membrane surface was observed by scanning electron microscopy after 96 h of PYR *** activities of superoxide dismutase(SOD)and catalase(CAT)increased throughout the *** contrast,the glutathione(GSH)content was increased at 24 h and 48 h of exposure and decreased at 96 ***,a significant increase in malondialdehyde(MDA)level was observed in *** after96 h of ***,PYR upregulated the HSP703,HSP705,GPx2,and ABAC15 gene expression in the 0.1–5-mg/L groups and downregulated the HSP704,HSP90,TGR,and ABCC52 mRNA levels at 96 h of *** results suggest that PYR may exert adverse effects on *** by inducing oxidative stress and changing the gene expression related to ABC transporters and stress,which may enrich the understanding of the toxicity mechanism of PYR in aquatic organisms and provide reference data for aquatic ecological risk assessments.

关键词： pyraclostrobin Tetrahymena thermophila growth oxidative stress gene expression