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DNS of compressible turbulent boundary layer over a blunt wedge

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Science China(Physics,Mechanics & Astronomy) 2005年第2期48卷 129-141页

作者： LI xinliang fu Dexun MA Yanwan LNM Institute of Mechanics Chinese Academy of Sciences Beijing 100080 China LNM Institute of Mechanics Chinese Academy of Sciences Beijing 100080 China LNM Institute of Mechanics Chinese Academy of Sciences Beijing 100080 China

Direct numerical simulation of spatially evolving compressible boundary layer over a blunt wedge is performed in this paper. The free-stream Mach number is 6 and the disturbance source produced by wall blowing and suction is located downstream of the sound-speed point. Statistics are studied and compared with the results in incompressible flat-plate boundary layer. The mean pressure gradient effects on the vortex structure are studied.

关键词： boundary layer, transition, direct numerical simulation, compressible turbulence.

基于创新能力培养的兽医免疫学综合实验教学体系构建

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科教导刊 2024年第11期 57-59页

作者：刘文俊付新亮曹楠仲恺农业工程学院动物科技学院广东广州510225

科技是第一生产力、人才是第一资源、创新是第一动力。创新能力是当代青年的核心竞争力,为适应新时代的要求,培养高质量应用型畜牧兽医人才,文章在总结当前兽医免疫学实验教学中存在的问题,整合和优化实验内容的基础上,对如何构建研究... 详细信息

科技是第一生产力、人才是第一资源、创新是第一动力。创新能力是当代青年的核心竞争力,为适应新时代的要求,培养高质量应用型畜牧兽医人才,文章在总结当前兽医免疫学实验教学中存在的问题,整合和优化实验内容的基础上,对如何构建研究设计性综合实验教学体系,如何改变过去验证性的传统教学模式,提高学生的创新能力等方面进行了探讨。

关键词：动物免疫学实验教学改革创新能力培养

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Stabilization of Cu/Ni Alloy Nanoparticles with Graphdiyne Enabling Efficient CO_(2) Reduction

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Chemical Research in Chinese Universities 2021年第6期37卷 1328-1333页

作者： fu xinliang ZHU Aonan CHEN Xiaojie ZHANG Shifu WANG Mei YUAN Mingjian Key Laboratory of Advanced Energy Materials Chemistry Ministry of EducationRenewable Energy Conversion and Storage Center(RECAST)College of ChemistryNankai UniversityTianjin300071P.R.China Key Laboratory of Marine Chemistry Theory and Technology Ministry of EducationCollege of Chemistry and Chemical EngineeringOcean University of ChinaQingdao266100P.R.China

Electrocatalysis has become an attractive strategy for the artificial reduction of CO_(2) to high-value ***,the design and development of highly selective and stable non-noble metal electrocatalysts that convert CO_(2) to CO are still a *** a new type of two-dimensional carbon material,graphdiyne(GDY),is rarely used to explore the application in carbon dioxide reduction reaction(CO_(2)RR).Therefore,we tried to use GDY as a substrate to stabilize the copper-nickel alloy nanoparticles(NPs)to synthesize Cu/Ni@***/Ni@GDY requires an overpotential(−0.61 V)to 10 mA/cm^(2) for the formation of CO,and it shows better activity than Au and Ag,achieving a higher Faraday efficiency of about 95.2%and high stability of about 26 h at an overpotential(−0.70 V).The electronic interaction between GDY substrate and Cu/Ni alloy NPs and the large specific surface area of GDY is responsible for the high performance.

关键词： Graphdiyne(GDY) Carbon dioxide reduction reaction(CO_(2)RR) CO Electrocatalysis Cu/Ni alloy nanoparticle

CoS_(2)nanowires supported graphdiyne for highly efficient hydrogen evolution reaction

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Journal of Energy Chemistry 2021年第9期30卷 272-278页

作者： Wangjing Xie Kang Liu Guodong Shi xinliang fu Xiaojie Chen Zixiong Fan Min Liu Mingjian Yuan Mei Wang Key Laboratory of Marine Chemistry Theory and Technology (Ministry of Education)College of Chemistry and Chemical EngineeringOcean University of ChinaQingdao 266100China Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education)Renewable Energy Conversion and Storage Center(RECAST)College of ChemistryNankai University300071 TianjinChina School of Physics and Electronics State Key Laboratory of Powder MetallurgyCentral South UniversityChangsha 410083China College of Science Henan University of TechnologyZhengzhou 450001China

Transition metal sulfides are an important category for hydrogen evolution reaction(HER).However,only few edge unsaturated sulfurs functionalize as catalytic sites,which has dramatically limited the catalytic activity and *** this work,planar unsaturated sulfurs in(211)plane of the CoS_(2)nanowires have been successfully activated through constructing Graphdiyne-CoS_(2)heterojunction *** corresponding electrons transfer energy barriers for these planar unsaturated sulfurs have been significantly diminished,which are induced by the synergetic effects of the sp~1 hybridized carbons and unsaturated planar *** addition,DFT simulations reveal the synergetic effects of the sp~1 hybridized carbons and unsaturated planar sulfurs can promote electron transfer kinetics of the key step,VolmerHeyrovsky step,of the *** expected,the Graphdiyne-CoS_(2)heterojunction nanocomposites exhibit superior HER catalytic performance with low overpotential of 97 mV at 10 mA cm^(-2),and the Tafel slope of 56 mV dec^(-1).furthermore,the heterojunction shows outstanding stability as well due to the pr tection of the Graphdiyne(GDY).The approach thus paves the way for the further efficient transition metal disulfides catalyst manufactures.

关键词： Graphdiyne Electrocatalysts Hydrogen evolution reaction DFT simulation 2D carbon material

白术多糖通过花生四烯酸代谢通路缓解环磷酰胺诱导的小鼠肾损伤

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食品工业科技 2024年第10期45卷 325-334页

作者：李冰心龚淑影许丹宁李婉雁曹楠付新亮江丹莉洪龙胜余梓榆白帅飞田允波仲恺农业工程学院动物科技学院广东广州510225

本研究旨在探讨白术多糖(PAMK)对环磷酰胺(CTX)诱导的小鼠肾脏损伤的影响及潜在的作用机制。选用100只42~43日龄雄性C57BL/6小鼠随机分为四组,白术多糖(PAMK)组和白术多糖+环磷酰胺(PAMK+CTX)组灌胃200 mg/kg PAMK,1次/d;对照(Control)... 详细信息

本研究旨在探讨白术多糖(PAMK)对环磷酰胺(CTX)诱导的小鼠肾脏损伤的影响及潜在的作用机制。选用100只42~43日龄雄性C57BL/6小鼠随机分为四组,白术多糖(PAMK)组和白术多糖+环磷酰胺(PAMK+CTX)组灌胃200 mg/kg PAMK,1次/d;对照(Control)组和环磷酰胺(CTX)组给予等量的生理盐水。实验第25~27 d,CTX组和PAMK+CTX组腹腔注射100 mg/kg CTX,1次/d;Control组和PAMK组注射等量生理盐水。实验第35 d采集肾脏进行组织学观察,氧化应激检测和转录组测序。结果显示,与CTX组相比,PAMK+CTX组肾脏损伤有所缓解;肾脏中丙二醛量显著下降(P<0.05)、谷胱甘肽过氧化物酶、超氧化物歧化酶、过氧化氢酶活性和总抗氧化能力显著升高(P<0.05)。为了进一步探究PAMK缓解CTX诱导肾损伤的调控机制,本实验进行了肾脏转录组测序。结果显示,在Control组vs CTX组和CTX组vs PAMK+CTX组分别鉴定到493个和333个差异表达基因(DEGs)。两组DEGs功能富集分析结果发现DEGs富集在花生四烯酸代谢等信号通路。花生四烯酸通路相关基因检测结果显示,与Control组相比,CTX组Cyp2b9、PTGS1、NF-κB和Mfsd2a mRNA表达量显著升高(P<0.05);与CTX组相比,PAMK+CTX组Cyp2c65、BCL6 mRNA表达量显著升高(P<0.05),Cyp2b9、PTGS1和Mfsd2a mRNA表达量显著降低(P<0.05)。综上所述,PAMK可能通过花生四烯酸代谢通路缓解小鼠肾脏氧化应激,从而降低CTX诱导的小鼠肾脏损伤。

关键词：白术多糖小鼠肾脏损伤环磷酰胺转录组测序

马岗鹅早期发育阶段腿肌lncRNA-miRNA-mRNA网络构建与分析

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中国家禽 2024年第9期46卷 34-45页

作者：洪龙胜许丹宁李婉雁曹楠付新亮田允波李玉谷李冰心仲恺农业工程学院动物科技学院广东广州510225 华南农业大学兽医学院广东广州510642

为探究非编码RNA对鹅骨骼肌生长发育的调控机制,试验以20胚龄和孵化后3日龄马岗鹅的腿部肌肉作为研究对象,通过HE染色观察不同阶段腿肌组织结构差异,并通过RNA测序探讨可能影响腿肌生长发育的RNAs。3日龄马岗鹅腿肌相较于20胚龄显示出... 详细信息

为探究非编码RNA对鹅骨骼肌生长发育的调控机制,试验以20胚龄和孵化后3日龄马岗鹅的腿部肌肉作为研究对象,通过HE染色观察不同阶段腿肌组织结构差异,并通过RNA测序探讨可能影响腿肌生长发育的RNAs。3日龄马岗鹅腿肌相较于20胚龄显示出了完整的肌纤维结构,肌纤维轮廓清晰;两阶段内共有2289个差异表达mRNAs,17个差异表达miRNAs和192个差异表达lncRNAs;差异表达基因在骨骼肌生长和发育相关的通路中显著富集,如细胞周期、FoxO信号通路和MAPK通路等;研究通过预测差异表达的mRNA和lncRNA与miRNA的靶向关系,成功构建一个与骨骼肌生长发育密切相关的ceRNA网络,其中36个lncRNAs、10个miRNAs被预测可以调控CCNB2、SHC4、ANGPT2、NRK、SLC25A4、WHAMM、FREM1、HMMR、PHKG1、DUSP1和COL1A2等与腿肌生长发育相关的基因。研究结果揭示了lncRNA和miRNA在鹅腿肌生长和发育中的潜在功能和机制,为进一步的研究提供了参考。

关键词：马岗鹅全转录组分析 ceRNA 腿肌

Import of Rift Valley fever to China:a potential new threat?

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Virologica Sinica 2016年第5期31卷 454-456页

作者： xinliang fu Lifang Wang Bo Fang Ruirui Ma Yun Zheng San Huang Pei Zhou Zongxi Cao Jin Tian Shoujun Li Guihong Zhang College of Veterinary Medicine South China Agricultural UniversityGuangzhou 510642China State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150009China Institute of Animal Husbandry and Veterinary Medicine Hainan Academy of Agricultural SciencesHaikou 571100China Key Laboratory of Zoonosis Prevention and Control of Guangdong Province Guangzhou 510642China Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases Guangzhou 510642China

Several recent studies in Virologica Sinica and other journals have highlighted the enormous international challenge of emerging arboviral diseases,such as Zika virus disease,dengue,and chikungunya(Islam et al.,2015;Maurice et al.,2015;Dai et al.,2016;Deng et al.,2016;Song et al.,2016;Wang et al.,2016;Xu et al.,2016;Zhang et al.,2016;Zhou

关键词： RVF a potential new threat Import of Rift Valley fever to China

Synthesis of Spiro Indazole-tetrahydrothiophenes via Sulfa-Michael/aldol Cascade Reactions

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Synthesis of Spiro Indazole-tetrahydrothiophenes via Sulfa-M...

NoV P颗粒嵌合PEDV S1 基因多表位抗原的制备

中国化学会第十二届全国物理有机化学学术会议

作者： fu xinliang Yu Xianyong Key Laboratory of Theoretical Organic Chemistry and functional Molecules Ministry of EducationSchool of Chemistry and Chemical EngineeringHunan University of Science and Technology

＜正＞The sulfa-Michael/aldol cascade reaction of 5-arylmethylidene-1-phenyl-6,7-dihydro-1 H-indazol-4（5 H）-ones and 1,4-dithiane-2,5-diol afforded novel 2’-（4-aryl）-4’-hydroxy-1-phenyl-4’,5’,6,7-tetrahydro-2’H-spiro[indazole-5,3’-thiophen]-4（1 H）-ones in moderate *** structures of all the products were

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仲恺农业工程学院学报 2024年第2期37卷 12-18页

作者：章程罗沛周德荣付新亮阳佑天黄运茂刘文俊仲恺农业工程学院动物科技学院广东广州510225 广东省水禽健康养殖重点实验室广东广州510225 佛山科学技术学院医药工程学院广东佛山528225

为制备诺如病毒(Norovirus,NoV)P颗粒嵌合猪流行性腹泻病毒(Porcine Epidemic Diarrhea Virus,PEDV)S1基因多表位的抗原.本研究通过设计合成PEDV S1多表位基因序列,酶切后连接到诺如病毒P基因重组pGEX-4T-1载体,将载体转化至大肠杆菌DH... 详细信息

为制备诺如病毒(Norovirus,NoV)P颗粒嵌合猪流行性腹泻病毒(Porcine Epidemic Diarrhea Virus,PEDV)S1基因多表位的抗原.本研究通过设计合成PEDV S1多表位基因序列,酶切后连接到诺如病毒P基因重组pGEX-4T-1载体,将载体转化至大肠杆菌DH5α感受态细胞后,提取质粒进行双酶切验证和测序.将测序正确的重组质粒转化至BL21表达菌体,使用不同浓度异丙基-β-D-硫代半乳糖苷(Isopropyl-β-D-thiogalactopyranoside,IPTG)在不同诱导时间下进行原核表达,确定最佳诱导条件,并对重组蛋白进行可溶性分析.使用鼠源谷胱甘肽-S-转移酶(Glutathione-S-transferase,GST)单抗和PEDV阳性血清通过蛋白免疫印迹试验检测纯化后的重组蛋白.重组质粒NoV P-partical-PEDV-SE经双酶切鉴定获得大小约510 bp的目的基因条带,进一步测序结果证明重组质粒构建成功.诱导表达的重组蛋白分子质量约为79 ku,与预期大小一致.使用1.0 mmol/L IPTG在37℃条件下培养2 h作为最佳诱导条件,对超声破碎后的菌体进行检测,试验结果显示重组蛋白可在上清液中表达.鼠源GST单抗与PEDV阳性血清均能特异性地识别纯化后的重组蛋白,表明该重组蛋白具有良好的反应原性.本研究通过对NoV P-partical-PEDV-SE蛋白原核表达及纯化,成功获得大量纯度较高的重组蛋白,为进一步制备猪流行性腹泻多表位口服疫苗,实现对PEDV的有效预防奠定基础.

关键词：诺如病毒P颗粒猪流行性腹泻病毒蛋白免疫原性疫苗