检索结果-南通市图书馆

CSPP1 stabilizes microtubules by capping both plus and minus ends

Journal of Molecular Cell Biology 2024年第2期16卷 36-46页

作者： Zhikai wang Wenwen wang Shuaiyu Liu Fengrui Yang Xu Liu Shasha hua Lĳuan Zhu Aoqing Xu Donald LHill Dongmei wang Kai Jiang Jennifer Lippincott-Schwartz xing Liu Xuebiao Yao MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics Center for Advanced Interdisciplinary Science and Biomedicine of IHMUniversity of Science and Technology of China School of Life SciencesHefei 230027China Anhui Key Laboratory for Cellular Dynamics and Chemical Biology Hefei National Research Center for Interdisciplinary Sciences at the MicroscaleHefei 230027China Frontier Science Center for Immunology and Metabolism Medical Research InstituteWuhan UniversityWuhan 430071China Comprehensive Cancer Center University of AlabamaBirminghamAL 35233USA Janelia Research Campus Howard Hughes Medical InstituteAshburnVA 20147USA

Although the dynamic instability of microtubules(MTs)is fundamental to many cellular functions,quiescent MTs with unattached free distal ends are commonly present and play important roles in various events to power cellular ***,how these free MT tips are stabilized remains poorly ***,we report that centrosome and spindle pole protein 1(CSPP1)caps and stabilizes both plus and minus ends of static ***-time imaging of laser-ablated MTs in live cells showed deposition of CSPP1 at the newly generated MT ends,whose dynamic instability was concomitantly ***,MT ends in CSPP1-overexpressing cells were hyper-stabilized,while those in CSPP1-depleted cells were much more *** CSPP1-elicited stabilization of MTs was demonstrated to be achieved by suppressing intrinsic MT catastrophe and restricting ***,CSPP1-bound MTs were resistant to mitotic centromere-associated kinesin-mediated *** findings delineate a previously uncharacterized CSPP1 activity that integrates MT end capping to orchestrate quiescent MTs.

关键词： CSPP1 microtubule plus end minus end capping

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Mast cell degranulation-triggered by SARS-CoV-2 induces tracheal-bronchial epithelial inflammation and injury

引用

Virologica Sinica 2024年第2期39卷 309-318页

作者： Jian-Bo Cao Shu-Tong Zhu Xiao-Shan huang xing-Yuan wang Meng-Li Wu Xin Li Feng-Liang Liu Ling Chen Yong-Tang Zheng Jian-hua wang Guangzhou Institutes of Biomedicine and Health Chinese Academy of SciencesGuangzhou 510530China School of Life Sciences and Medicine University of Science and Technology of ChinaHefei 230026China Key Laboratory of Bioactive Peptides of Yunnan Province Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of SciencesKIZCUHK Joint Laboratory of Bioresources and Molecular Research in Common DiseasesCenter for Biosafety Mega-ScienceKunming Institute of ZoologyChinese Academy of SciencesKunming 650223China University of Chinese Academy of Sciences Beijing 101408China

SARS-CoV-2 infection-induced hyper-inflammation is a key pathogenic factor of *** research,along with others',has demonstrated that mast cells(MCs)play a vital role in the initiation of hyper-inflammation caused by *** previous study,we observed that SARS-CoV-2 infection induced the accumulation of MCs in the peri-bronchus and bronchioalveolar-duct junction in humanized ***,we found that MC degranulation triggered by the spike protein resulted in inflammation in alveolar epithelial cells and capillary endothelial cells,leading to subsequent lung *** trachea and bronchus are the routes for SARS-CoV-2 transmission after virus inhalation,and inflammation in these regions could promote viral *** are widely distributed throughout the respiratory ***,in this study,we investigated the role of MCs and their degranulation in the development of inflammation in tracheal-bronchial *** analyses showed the accumulation and degranulation of MCs in the peri-trachea of humanized mice infected with *** degranulation caused lesions in trachea,and the formation of papillary hyperplasia was *** transcriptome analysis in bronchial epithelial cells,we found that MC degranulation significantly altered multiple cellular signaling,particularly,leading to upregulated immune responses and *** administration of ebastine or loratadine effectively suppressed the induction of inflammatory factors in bronchial epithelial cells and alleviated tracheal injury in *** together,our findings confirm the essential role of MC degranulation in SARS-CoV-2-induced hyper-inflammation and the subsequent tissue ***,our results support the use of ebastine or loratadine to inhibit SARS-CoV-2-triggered degranulation,thereby preventing tissue damage caused by hyper-inflammation.

关键词： SARS-CoV-2 Mast cell(MC) Bronchial epithelial cell Inflammation Tracheal injury

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Transient diabetes mellitus with ABCC8 variant successfully treated with sulfonylurea:Two case reports and review of literature

引用

World Journal of Diabetes 2024年第8期15卷 1811-1819页

作者： Ling-hua Shen Yan Cui Dong-Xia Fu Wei Yang Sheng-Nan Wu Hui-Zhen wang Hai-hua Yang Yong-xing Chen Hai-Yan Wei Department of Endocrinology and Metabolism Henan Key Laboratory of Children's Genetics and Metabolic DiseasesChildren's Hospital Affiliated to Zhengzhou UniversityHenan Children's Hospital Zhengzhou Children's HospitalZhengzhou 450018Henan ProvinceChina

BACKGROUND Transient neonatal diabetes mellitus(TNDM)is a rare form of diabetes mellitus that usually presents within the first 6 mo of *** often enter remission within several months,although relapse can occur later in *** in the ABCC8 gene,which encodes the sulfonylurea receptor 1 of the ATP-sensitive potassium channel in pancreatic beta cells,are associated with TNDM and permanent neonatal *** study describes a novel de novo c.3880C>T heterozygous ABCC8 variant that causes TNDM and can be treated with sulfonylurea *** SUMMARY We retrospectively analyzed 2 Chinese patients with TNDM who were diagnosed,treated,or referred for follow-up between September 2017 and September *** patients were tested for mutations using targeted next-generation *** with neonatal diabetes mellitus caused by a c.3880C>T heterozygous missense variant in the ABCC8 gene have not been reported *** children had an onset of post-infectious diabetic ketoacidosis,which is worth *** a follow-up visit after discontinuing insulin injection,oral glyburide was found to be effective with no adverse *** Early genetic testing of neonatal diabetes mellitus aids in accurate diagnosis and treatment and helps avoid daily insulin injections that may cause pain.

关键词： Neonatal diabetes mellitus ABCC8 Sulfonylurea receptors 1 KATP channels Sulfonylurea Glyburide Insulinulin Case report

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Antitumor activity of an hTERT promoter-regulated tumor-selective oncolytic adenovirus in human hepatocellular carcinoma

引用

World Journal of Gastroenterology 2006年第47期12卷 7613-7620页

作者： Chang-Qing Su xing-hua wang Jie Chen Yong-Jing Liu Wei-Guo wang Lin-Fang Li Meng-Chao Wu Qi-Jun Qian Laboratory of Viral and Gene Therapy Eastern Hepatobiliary Surgery Hospital Second Military Medical University Shanghai 200438 China Xinyuan Institute of Medicine and Biotechnology College of Life Science Zhcjiang Sci-T~ch University Hangzhou 310018 Zhejiang Province China Changhai Hospital Second Military Medical University Shanghai 200438 China Jiangsu Key Laboratory for Molecular and Medical Bioteclmology Life Science College Nanjing Normal University Nanjing 210097 Jiangsu Province China

AIM: To construct a tumor-selective replication-competent adenovirus (RCAd), SG300, using a modified promoter of human telomerase reverse transcriptase (hTERT). METHODS: The antitumor efficacy of SG300 in hepatocellular carcinoma was assessed in vitro and in vivo. In vitro cell viability by MTT assay was used to assess the tumor-selective oncolysis and safety features of SG300, and in vivo antitumor activity of SG300 was assessed in established hepatocellular carcinoma models in nude mice. RESULTS: SG300 could lyse hepatocellular carcinoma cells at a low multiplicity of infection (MOI), but could not affect growth of normal cells even at a high MOI. Both in Hep3B and SMMC-7721 xenograft models of hepatocellular carcinoma, SG300 had an obvious antitumor effect, resulting in a decrease in tumor volume. Its selective oncolysis to tumor cells and safety to normal cells was also superior to that of ONYX-015. Pathological examination of tumor specimens showed that SG300 replicated selectively in cancer cells and resulted in apoptosis and necrosis of cancer cells. CONCLUSION: hTERT promoter-regulated replicativeadenovirus SG300 has a better cancer-selective replication-competent ability, and can specifically kill a wide range of cancer cells with positive telomerase activity, and thus has better potential for targeting therapy of hepatocellular carcinoma.

关键词： Virotherapy Oncolytic adenovirus Human telomerase reverse transcriptase Hepatocellular carcinoma Animal tumor model

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Clinical manifestation,lifestyle,and treatment patterns of chronic erosive gastritis:A multicenter real-world study in China

引用

World Journal of Gastroenterology 2024年第9期30卷 1108-1120页

作者： Ying-Yun Yang Ke-Min Li Gui-Fang Xu Cheng-Dang wang hua Xiong Xiao-Zhong wang Chun-Hui wang Bing-Yong Zhang Hai-xing Jiang Jing Sun Yan Xu Li-Juan Zhang Hao-Xuan Zheng Xiang-Bin xing Liang-Jing wang Xiu-Li Zuo Shi-Gang Ding Rong Lin Chun-Xiao Chen xing-Wei wang Jing-Nan Li Department of Gastroenterology Peking Union Medical College HospitalPeking Union Medical CollegeChinese Academy of Medical SciencesBeijing 100730China Department of Gastroenterology Nanjing Drum Tower HospitalNanjing 21000Jiangsu ProvinceChina Department of Gastroenterology The First Affiliated Hospital of Fujian Medical UniversityFuzhou 350005Fujian ProvinceChina Department of Gastroenterology Renji HospitalShanghai 200127China Department of Gastroenterology Union Hospital of Fujian Medical UniversityFuzhou 350000Fujian ProvinceChina Department of Gastroenterology West China Hospital of Sichuan UniversityChengdu 610041Sichuan ProvinceChina Department of Gastroenterology and Hepatology The People’s Hospital of Zhengzhou UniversityThe Henan Provincial People’s HospitalZhengzhou 450003Henan ProvinceChina Department of Gastroenterology The First Affiliated Hospital of Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina Ruijin Hospital Shanghai Jiaotong University School of Medicine Shanghai Jiao Tong UnivesrityRuijin HospitalSchool MedicineShanghai 200025China Department of Gastroenterology and Hepatology China-Japan Union Hospital of Jilin UniversityChangchun 130033Jilin ProvinceChina Department of Gastroenterology Fujian Medical University Union HospitalFuzhou 350001Fujian ProvinceChina Department of Gastroenterology Nanfang HospitalGuangzhou 510080Guangdong ProvinceChina Department of Gastroenterology The First Affiliated HospitalSun Yat-sen UniversityGuangzhou 510080Guangdong ProvinceChina Department of Gastroenterology The Second Affiliated Hospital Zhejiang University School of MedicineHangzhou 310000Zhejiang ProvinceChina Department of Gastroenterology Shandong University Qilu HospitalJinan 250012Shandong ProvinceChina Department of Gastroenterology Peking University Third HospitalBeijing 100191China Department of Gastroenterology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022Hubei Pr

BACKGROUND Although chronic erosive gastritis(CEG)is common,its clinical characteristics have not been fully *** lack of consensus regarding its treatment has resulted in varied treatment *** To explore the clinical characteristics,treatment patterns,and short-term outcomes in CEG patients in *** We recruited patients with chronic non-atrophic or mild-to-moderate atrophic gastritis with erosion based on endoscopy and *** and treating physicians completed a questionnaire regarding history,endoscopic findings,and treatment plans as well as a follow-up questionnaire to investigate changes in symptoms after 4 wk of *** Three thousand five hundred sixty-three patients from 42 centers across 24 cities in China were *** pain(68.0%),abdominal distension(62.6%),and postprandial fullness(47.5%)were the most common presenting *** was classified as chronic non-atrophic in 69.9%of *** those with erosive lesions,72.1%of patients had lesions in the antrum,51.0%had multiple lesions,and 67.3%had superficial flat *** patients with epigastric pain,the combination of a mucosal protective agent(MPA)and proton pump inhibitor was more *** those with postprandial fullness,acid regurgitation,early satiety,or nausea,a MPA appeared more *** CEG is a multifactorial disease which is common in Asian patients and has non-specific *** may play a major role in its detection and *** should be individualized based on symptom profile.

关键词： Chronic erosive gastritis Symptom Endoscopic findings Treatment pattern Real-world

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano polystyrene microplastics could accumulate in Nile tilapia(Oreochromis niloticus):Negatively impacts on the intestinal and liver health through water exposure

引用

Journal of Environmental Sciences 2024年第3期137卷 604-614页

作者： Di Wu xing Lu Li-Xue Dong Juan Tian Jin Deng Lei Wei hua Wen Shan Zhong Ming Jiang School of Basic Medical Sciences Wuhan UniversityWuhan 430071China Key Laboratory of Freshwater Biodiversity Conservation Ministry of Agriculture and Rural AffairsYangtze River Fisheries Research InstituteChinese Academy of Fishery SciencesWuhan 430223China Hubei Province Key Laboratory of Allergy and Immunology Wuhan 430071China

Microplastics(MPs)have become a significant concern for their potential ***,the correlation between the size of plastic particles and their toxicity remains ***,we investigate the toxic effects of different sizes(80 nm,800 nm,8μm and 80μm)polystyrene MPs(PS-MPs)on the model organism Nile tilapia(Oreochromis niloticus).The results of bioluminescent imaging indicate that the 80 nm PS-MPs are more likely to invade the body.H&E staining shows severe damage on the intestinal villi and distinct hepatic steatosis in the 80 nm *** U labeling shows that the proliferation activity of intestinal and liver cells reduces significantly in the 80 nm *** gut microbiome analysis shows a severe imbalance of gut microbiota homeostasis in the 80 nm *** analysis of liver transcriptomics and metabolomics shows that the liver lipid metabolism is disordered in the 80 nm *** conclusion,this study confirms that the 80 nm PS-MPs are more likely to induce intestinal and liver *** the above lay the foundation for further study on the pathological damage of MPs to other organisms.

关键词： Microplastics(MPs) Intestinal toxicity Liver toxicity Gut microbiome Lipid metabolism

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Silent information regulator sirtuin 1 ameliorates acute liver failure via the p53/glutathione peroxidase 4/gasdermin D axis

引用

World Journal of Gastroenterology 2024年第11期30卷 1588-1608页

作者： xing-Nian Zhou Quan Zhang Hong Peng Yu-Jie Qin Yu-Hong Liu Lu wang Ming-Liang Cheng Xin-hua Luo Hong Li Department of Infectious Diseases The Affiliated Hospital of Guizhou Medical UniversityGuizhou Medical UniversityGuiyang 550004Guizhou ProvinceChina Department of Infectious Diseases Guizhou Provincial People's HospitalGuiyang 550001Guizhou ProvinceChina

BACKGROUND Acute liver failure(ALF)has a high mortality with widespread hepatocyte death involving ferroptosis and *** silent information regulator sirtuin 1(SIRT1)-mediated deacetylation affects multiple biological processes,including cellular senescence,apoptosis,sugar and lipid metabolism,oxidative stress,and *** To investigate the association between ferroptosis and pyroptosis and the upstream regulatory *** This study included 30 patients with ALF and 30 healthy individuals who underwent serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)testing.C57BL/6 mice were also intraperitoneally pretreated with SIRT1,p53,or glutathione peroxidase 4(GPX4)inducers and inhibitors and injected with lipopolysaccharide(LPS)/D-galactosamine(D-GalN)to induce *** D(GSDMD)^(-/-)mice were used as an experimental *** changes in liver tissue were monitored by hematoxylin and eosin ***,AST,glutathione,reactive oxygen species,and iron levels were measured using commercial ***-and pyroptosis-related protein and mRNA expression was detected by western blot and quantitative real-time polymerase chain ***1,p53,and GSDMD were assessed by immunofluorescence *** Serum AST and ALT levels were elevated in patients with ***1,solute carrier family 7a member 11(SLC7A11),and GPX4 protein expression was decreased and acetylated p5,p53,GSDMD,and acyl-CoA synthetase long-chain family member 4(ACSL4)protein levels were elevated in human ALF liver *** the p53 and ferroptosis inhibitor-treated and GSDMD^(-/-)groups,serum interleukin(IL)-1β,tumour necrosis factor alpha,IL-6,IL-2 and C-C motif ligand 2 levels were decreased and hepatic impairment was *** mice with GSDMD knockout,p53 was reduced,GPX4 was increased,and ferroptotic events(depletion of SLC7A11,elevation of ACSL4,and iron accumulation)were *** vitro,knockdown of p53 and overexpression of GPX

关键词： Silent information regulator sirtuin 1 Ferroptosis Pyroptosis p53/glutathione peroxidase 4/gasdermin D Acute liver failure

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Tuberous sclerosis complex combined with primary lymphedema:A case report

引用

World Journal of Clinical Cases 2024年第15期12卷 2642-2648页

作者： xing-Peng Li Xiao-Li Sun Xin Liu Zhe Wen Li-hua Jiang Yan Fu Yun-Long Yue Ren-Gui wang Department of Radiology Beijing Shijitan Hospital Affiliated to Capital Medical UniversityBeijing 100038China Department of Lymph Surgery Beijing Shijitan Hospital Affiliated to Capital Medical UniversityBeijing 100038China Department of Nuclear Medicine Beijing Shijitan Hospital Affiliated to Capital Medical UniversityBeijing 100038China Department of MRI Beijing Shijitan Hospital Affiliated to Capital Medical UniversityBeijing 100038China

BACKGROUND Tuberous sclerosis complex(TSC)and primary lymphedema(PLE)are both rare diseases,and it is even rarer for both to occur in the same *** this work,we have provided a detailed description of a patient's clinical presentation,imaging findings,and *** a retrospective analysis was conducted on 14 published relevant case *** SUMMARY A 16-year-old male came to our hospital for treatment due to right lower limb *** swelling is already present from *** patient’s memory had been progressively *** had occurred 1 year prior at an unknown *** patient was diagnosed with TSC combined with PLE through multimodal imaging examination:Computed tomography,magnetic resonance imaging,and *** patient underwent *** swelling of the patient's right lower limb significantly improved after *** did not *** taking antiepileptic drugs and *** TSC with PLE is a rare and systemic *** can detect lesions of this disease,which are important for diagnosis and treatment.

关键词： Tuberous sclerosis complex Lymphedema Sirolimus Multimodal imaging examination Case report

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

嵌入备件库存的设备运维闭环控制系统仿真研究

引用

系统仿真学报 2018年第10期30卷 3817-3825页

作者：李冰王星轩华王薛苑郑州大学管理工程学院河南郑州450001

高度可靠的设备运行维护系统是企业安全生产的关键与生命线。研究一类嵌入备件库存的设备运维控制系统。该系统由备件库存子系统、设备作业子系统和设备维修子系统以串并混合形式连接,从而形成多级闭环形态。设备运维闭环控制系统属于... 详细信息

高度可靠的设备运行维护系统是企业安全生产的关键与生命线。研究一类嵌入备件库存的设备运维控制系统。该系统由备件库存子系统、设备作业子系统和设备维修子系统以串并混合形式连接,从而形成多级闭环形态。设备运维闭环控制系统属于一类离散事件动态系统,用传统数学解析方法难以描述,利用Simulink仿真平台建立多级闭环设备运维系统仿真模型并进行数值实验,进而剖析系统设备故障率、维修通道规模及维修效率对系统的整体影响,并分析订货策略对备件库存成本的影响。

关键词：设备运维闭环控制系统排队网络数值仿真

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Mechanical performance of 22SiMn2TiB steel welded with lowtransformation- temperature filler wire and stainless steel filler wire

引用

Journal of Iron and Steel Research International 2024年第4期31卷 967-981页

作者： Zi-dong Lin Kai-jie Song Zhen Sun Zi-qian Zhu Xue-feng Zhao Constantinos Goulas Wei Ya xing-hua Yu School of Materials Science and Engineering Beijing Institute of TechnologyBeijing 100081China Beijing Institute of Technology Chongqing Innovation Center Chongqing 401120China Department of Design Production and Management Faculty of Engineering Technology.University of TwenteEnschede 7500 AETheNetherlands Rotterdam Additive Manufacture Fieldlab(RAMLAB) Rotterdam 3089 JWTheNetherlands

TX-80 low-transformation-temperature(LTT)welding wire was used to replace the traditional ER 307Si welding wire to realize the connection of 22SiMn2TiB armor steel in manual overlay *** previously existing issues,such as welding cracks,large welding deformation,and severe welding residual stress,were solved to ensure good strength and ductility *** particular,with the same welding conditions,TX-80 LTT wire eliminates welding *** reduces the welding deformation no matter the base pretreatment of pre-setting angle or no pre-setting *** comparison,it was found that the microstructure at the TX-80 weld is mainly composed of martensite and a small amount of retained *** contrast,the microstructure of the ER 307Si weld consists of a large amount of austenite and a small amount of skeleton-like *** variation trend of residual stress and microhardness from the weld to the base were investigated and compared with the mechanical properties of base *** TX-80 and the ER 307Si tensile samples elongation is 6.76%and 6.01%,while the ultimate tensile strengths are 877 and 667 MPa,*** average impact toughness at room temperature of the ER 307Si weld is 143.9 J/cm^(2),much higher than that of the TX-80 weld,which is only 36.7 J/cm^(2).The relationship between impact and tensile properties with microstructure species and distribution was *** addition,the fracture surface of the tensile and the impact samples was observed and *** dimples,fewer pores,larger radiation zone,and shear lips of TX-80 samples indicate better tensile ductility and worse impact toughness than those of ER 307Si weld.

关键词： Low-transformation-temperature material 22SiMn2TiB steel Welding deformation Residual stress Martensitic transformation Mechanical property

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：