检索结果-南通市图书馆

DMF-scMT-seq linking methylome and transcriptome within single cells with digital microfluidics

Science China Chemistry 2024年第6期67卷 2070-2078页

作者： xing Xu xi Zeng xin Lin Shiyan Lin Shanshan Liang Tian Tian Rui Su Jia Song Chaoyong Yang Collaborative Innovation Center of Chemistry for Energy Materials The MOE Key Laboratory of Spectrochemical Analysis and InstrumentationState Key Laboratory of Physical Chemistry of Solid SurfacesDepartment of Chemical BiologyCollege of Chemistry and Chemical EngineeringThe First Affiliated Hospital of Xiamen UniversityXiamen UniversityXiamen361005China Institute of Molecular Medicine Renji HospitalShanghai Jiao Tong University School of MedicineShanghai200127China Chemistry and Materials Science College Shanghai Normal UniversityShanghai200234China Chemistry and Biomedicine Innovation Center(ChemBIC) School of Chemistry and Chemical EngineeringNanjing UniversityNanjing210023China

Single-cell joint analysis of methylome and transcriptome reveals how the methylation regulates the transcriptional ***,traditional bench-top protocols for single-cell DNA methylation and RNA transcription co-detection are laborintensive,cost-ineffective and ***,we establish the DMF-sc MT-seq,a highly-efficient and cost-effective method to simultaneously analyze single-cell DNA methylation and transcriptional activity based on digital *** MT-seq automates the workflow of single-cell isolation,cellular hypotonic lysis,nucleic acid separation and methylome/transcriptome library construction in a contactless and addressable *** system ensures high accuracy(R>0.85),high gene detection ability(14,697 genes per cell at 4 million sequencing depth),and high CpG coverage(677,198 CpG sites per cell at 1million sequencing depth).By using DMF-sc MT-seq,the relationship of DNA methylation and RNA transcription under different genomic contexts is *** further apply DMF-sc MT-seq to study the dynamics of transcription regulation with methylation-inhibiting anti-tumor Decitabine,and identify the methylated promoter/gene body driven genes in response to Decitabine ***-sc MT-seq facilitates the construction of the correlation of DNA methylation and transcriptional activity at the single-cell level in a flexible,sensitive and accurate way,which is anticipated to be a powerful tool in studying single-cell biological systems.

关键词： single-cell analysis digital microfluidics methylome sequencing transcriptome sequencing

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Event-triggered-based distributed differential game guidance laws

引用

Kongzhi yu Juece/Control and Decision 2024年第11期39卷 3745-3754页

作者： xi, A-xing Cai, Yuan-Li Control Engineering Research Institute Xi’an Jiaotong University Xi’an 710049 China

To reduce computational loads and save communication resources of cooperative multi-missile systems based on the event-triggered mechanism, the distributed differential game guidance laws are investigated. First, the cooperative multi-missile interception problem is converted into multi-player zero-sum differential games. The proposed guidance laws are derived by addressing the couple event-triggered-based Hamilton-Jacobi-Bellman equation, which is solved by using the adaptive dynamic programming technique. Meanwhile, adaptive weight tuning laws and event-triggered conditions are designed. The proposed guidance laws can guarantee the stability of the nonlinear multi-missile system, which is proved using the Lyapunov method. Then, the estimated weights of the neural network and consensus tracking errors are ensured to be uniformly ultimately bounded. Besides, a minimal inter-sample time is established for avoiding the Zeno behavior. Finally, some experiments are provided to illustrate the effectiveness of the proposed guidance laws. Compared with traditional distributed differential game guidance laws, the proposed guidance law reduces the number of state updates of the cooperative system controller by 65.36 %. The computational burden and communication resources of the control system can be greatly reduced. © 2024 Northeast University. All rights reserved.

关键词： Lyapunov methods

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

国产大型CFB锅炉冷态试验中若干问题分析

引用

中国电力 2010年第2期43卷 56-59页

作者：毛晓飞殷星兰幸双喜江西省电力科学研究院江西南昌330096

随着国产循环流化床(CFB)锅炉的不断大型化,冷态试验在国产大型CFB锅炉的冷态调试中具有重要意义,冷态试验结果的准确性是保证CFB锅炉安全、稳定运行的基础。以国产某首台自主研发1 025 t/h CFB锅炉的冷态试验为例,针对风量测量装置标... 详细信息

随着国产循环流化床(CFB)锅炉的不断大型化,冷态试验在国产大型CFB锅炉的冷态调试中具有重要意义,冷态试验结果的准确性是保证CFB锅炉安全、稳定运行的基础。以国产某首台自主研发1 025 t/h CFB锅炉的冷态试验为例,针对风量测量装置标定试验、炉膛布风板阻力特性试验、炉膛料层阻力及临界流化风量试验、炉膛布风均匀性试验和外置床布风板阻力特性试验中的一些关键点和出现的若干问题进行了分析,并从实际经验和设计原理出发提出了解决方法和建议,可为大型CFB锅炉的冷态调试和热态稳定运行提供参考。

关键词：循环流化床锅炉冷态试验布风板阻力料层阻力

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Monotonic mechanical behaviour of compacted completely decomposed granite with various inclusion levels of incineration bottom ash

引用

Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2024年第8期25卷 670-679页

作者： Han-Lin WANG Cheng-shuang YIN Qian-Yi ZHANG Qi-Wei LIU Zhen-Yu YIN Dong-xing XUAN Askar KHASANOV Research Center for Advanced Underground Space Technologies of Hunan University Changsha 410082China Key Laboratory of Building Safety and Energy Efficiency of the Ministry of Education Hunan UniversityChangsha 410082China College of Civil Engineering Hunan UniversityChangsha 410082China School of Civil Engineering Zhengzhou UniversityZhengzhou 450001China Department of Civil and Environmental Engineering The Hong Kong Polytechnic UniversityHung HomKowloonHong KongChina Department of Applied and Theoretical Mechanics Samarkand State UniversitySamarkandUzbekistan

1 Introduction Completely decomposed granite(CDG)is widely distributed in South China(Xu et al.,2022;Wang et al.,2023).The parent granite rock mass gradually loses features during the weathering process,and thus needs to be reinforced when used(Lan et al.,2003;Dassekpo et al.,2017;Alamanis et al.,2021).On the other hand,considering the huge demand for construction materials and limited natural resources,it is increasingly important to fully utilize solid waste(Gruhler et al.,2019;Anagnostopoulos et al.,2020;Jiang et al.,2022,2023a,2023b).With high strength and environment-friendly characteristics,incineration bottom ash(IBA)seems to be a suitable reinforcement material for CDG(Ahmed and Khalid,2011;Alhassan and Tankó,2012;Toraldo et al.,2013;Lynn et al.,2017;Xuan et al.,2018;Tang et al.,2020).

关键词： incineration behaviour utilize

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Gene characterization and phylogenetic analysis of four mitochondrial genomes in Caenogastropoda

引用

Acta Oceanologica Sinica 2024年第2期43卷 137-150页

作者： Jiangyong Qu Wanqi Yang xindong Teng Li Xu Dachuan Zhang Zhikai xing shuang Wang xiumei Liu Lijun Wang Xumin Wang College of Life Science Yantai UniversityYantai 264005China Qingdao International Travel Healthcare Center Qingdao 266071China Qingdao Dagang Customs Qingdao 266071China

Caenogastropoda is a highly diverse group,containing~60%of all existing *** in this subclass predominantly inhabit marine environments and have a high ecological and economic *** to the increase in relevant phylogenetic studies,our understanding of between species relatedness in Caenogastropoda has ***,the biodiversity,taxonomic status,and phylogenetic relationships of this group remain *** the present study,we performed next-generation sequencing of four complete mitochondrial genomes from three families(Buccinidae,Columbellidae,and Cypraeidae)and the four mitogenomes were classical circular structures,with a length of 16177 bp in Volutharpa ampullacea,16244 bp in Mitrella albuginosa,16926bp in Mauritia arabica asiatica and 15422 bp in Erronea *** composition analysis indicated that whole sequences were biased toward A and *** compared them with 171 complete mitochondrial genomes of *** phylogenetic relationship of Caenogastropoda derived from Maximum Likelihood(ML)and Bayesian Inference(BI)trees constructed based on CDS sequences was consistent with the results of traditional morphological analysis,with all three families showing close *** study supported Caenogastropoda at the molecular level as a separate clade of *** to our divergence time estimations,Caenogastropoda was formed during the middle Triassic period(~247.2–237 Ma).Our novel mitochondrial genomes provide evidence for the speciation of Caenogastropoda in addition to elucidating the mitochondrial genomic evolution of this subclass.

关键词： mitochondrial genome phylogenetic analysis Caenogastropoda

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Graphitic carbon nitride nanosheets mitigate cadmium toxicity in Glycine max *** promoting cadmium retention in root and improving photosynthetic performance

引用

Journal of Environmental Sciences 2024年第5期139卷 543-555页

作者： Kai Xu Yunyu Guo Chenghua xing Ronglong Fu Bin Zou Rongchuan Liu Luyi Cai Jianfang Yan xi-Lin Wu Miaozhen Cai College of Geography and Environmental Sciences Zhejiang Normal UniversityJinhua 321004China College of Agriculture Jinhua PolytechnicJinhua 321007China

Cadmium(Cd)pollution poses a serious threat to plant growth and *** have shown great application potential for alleviation of Cd toxicity to *** this study,we applied graphitic carbon nitride nanosheets(g-C_(3)N_(4)NSs)for alleviation of Cd-toxicity to soybean(Glycine max L.).The g-C_(3)N_(4)NSs supplementation significantly improved plant growth and reduced oxidative damage in the Cd-toxicated soybean seedlings through hydroponic ***,the g-C_(3)N_(4)NSs dynamically regulated the root cell wall(RCW)components by increasing pectin content and modifying its demethylation via enhancing pectin methylesterase(PME)activity,therefore greatly enhanced stronger RCW-Cd retention(up to 82.8%)and reduced Cd migration to the ***,the g-C_(3)N_(4)NSs reversed the Cd-induced chlorosis,increased photosynthetic efficiency because of enhancement in F_v/F_mration,Y(Ⅱ)and sugars *** results provide new insights into the alleviation of Cd toxicity to plants by g-C_(3)N_(4)NSs,and shed light on the application of low-cost and environmental-friendly carbon-based NMs for alleviating heavy metal toxicity to plants.

关键词： Cadmium Carbon nitride nanosheets Cell wall Pectin Pectin methylesterase Soybean

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Multichannel microneedle dry electrode patches for minimally invasive transdermal recording of electrophysiological signals

引用

Microsystems & Nanoengineering 2024年第3期10卷 119-136页

作者： Zhengjie Liu xingyuan Xu shuang Huang xinshuo Huang Zhibo Liu Chuanjie Yao Mengyi He Jiayi Chen Hui-jiuan Chen Jing Liu xi xie State Key Laboratory of Optoelectronic Materials and Technologies School of Electronics and Information TechnologyGuangdong Province Key Laboratory of Display Material and TechnologySun Yat-Sen UniversityGuangzhouChina School of Biomedical Engineering Sun Yat-Sen UniversityGuangzhouChina The First Affiliated Hospital of Sun Yat-Sen University GuangzhouChina

The collection of multiple-channel electrophysiological signals enables a comprehensive understanding of the spatial distribution and temporal features of electrophysiological *** approach can help to distinguish the traits and patterns of different ailments to enhance diagnostic *** array electrodes,which can penetrate skin without pain,can lessen the impedance between the electrodes and skin;however,current microneedle methods are limited to single channels and cannot achieve multichannel collection in small ***,a multichannel(32 channels)microneedle dry electrode patch device was developed via a dimensionality reduction fabrication and integration approach and supported by a self-developed circuit system to record weak electrophysiological signals,including electroencephalography(EEG),electrocardiogram(ECG),and electromyography(EMG)*** microneedles reduced the electrode–skin contact impedance by penetrating the nonconducting stratum corneum in a painless *** multichannel microneedle array(MMA)enabled painless transdermal recording of multichannel electrophysiological signals from the subcutaneous space,with high temporal and spatial resolution,reaching the level of a single microneedle in terms of signal *** MMA demonstrated the detection of the spatial distribution of ECG,EMG and EEG signals in live rabbit models,and the microneedle electrode(MNE)achieved better signal quality in the transcutaneous detection of EEG signals than did the conventional flat dry electrode *** work offers a promising opportunity to develop advanced tools for neural interface technology and electrophysiological recording.

关键词： needle rabbit electrode

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Extensive identification of landslide boundaries using remote sensing images and deep learning method

引用

China Geology 2024年第2期7卷 277-290页

作者： Chang-dong Li Peng-fei Feng xi-hui Jiang shuang Zhang Jie Meng Bing-chen Li Faculty of Engineering China University of GeoscienceWuhan 430074China Badong National Observation and Research Station of Geohazards China University of GeosciencesWuhan 430074China School of Mechanical Engineering and Electronic Information China University of GeosciencesWuhan 430074China College of Geology Engineering and Geomatics Chang’an UniversityXi’an 710054China

The frequent occurrence of extreme weather events has rendered numerous landslides to a global natural disaster *** is crucial to rapidly and accurately determine the boundaries of landslides for geohazards evaluation and emergency ***,the Skip Connection DeepLab neural network(SCDnn),a deep learning model based on 770 optical remote sensing images of landslide,is proposed to improve the accuracy of landslide boundary *** SCDnn model is optimized for the over-segmentation issue which occurs in conventional deep learning models when there is a significant degree of similarity between topographical geomorphic *** exhibits notable improvements in landslide feature extraction and semantic segmentation by combining an enhanced Atrous Spatial Pyramid Convolutional Block(ASPC)with a coding structure that reduces model *** experimental results demonstrate that SCDnn can identify landslide boundaries in 119 images with MIoU values between 0.8and 0.9;while 52 images with MIoU values exceeding 0.9,which exceeds the identification accuracy of existing *** work can offer a novel technique for the automatic extensive identification of landslide boundaries in remote sensing images in addition to establishing the groundwork for future inve stigations and applications in related domains.

关键词： Geohazard Landslide boundary detection Remote sensing image Deep learning model Steep slope Large annual rainfall Human settlements Infrastructure Agricultural land Eastern Tibetan Plateau Geological hazards survey engineering

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Microarrow sensor array with enhanced skin adhesion for transdermal continuous monitoring of glucose and reactive oxygen species

引用

Bio-Design and Manufacturing 2024年第1期7卷 14-30页

作者： xinshuo Huang Baoming Liang Shantao Zheng Feifei Wu Mengyi He shuang Huang Jingbo Yang Qiangqiang Ouyang Fanmao Liu Jing Liu Hui-jiuan Chen xi xie State Key Laboratory of Optoelectronic Materials and Technologies School of Electronics and Information TechnologyGuangdong Province Key Laboratory of Display Material and TechnologySun Yat-Sen UniversityGuangzhou 510006China School of Biomedical Engineering Sun Yat-Sen UniversityShenzhen 518107China Pazhou Lab Guangzhou 510330China The First Affiliated Hospital of Sun Yat-Sen University Guangzhou 510006China

Conventional blood sampling for glucose detection is prone to cause pain and fails to continuously record glucose fluctuations in *** glucose monitoring based on implantable electrodes could induce pain and potential tissue inflammation,and the presence of reactive oxygen species(ROS)due to inflammationmay affect glucose *** technology is less invasive,yet microneedle adhesion with skin tissue is *** this work,we developed a microarrow sensor array(MASA),which provided enhanced skin surface adhesion and enabled simultaneous detection of glucose and H_(2)O_(2)(representative of ROS)in interstitial fluid in *** microarrows fabricated via laser micromachining were modified with functional coating and integrated into a patch of a three-dimensional(3D)microneedle *** to the arrow tip mechanically interlocking with the tissue,the microarrow array could better adhere to the skin surface after penetration into *** MASA was demonstrated to provide continuous in vivo monitoring of glucose and H_(2)O_(2) concentrations,with the detection of H_(2)O_(2) providing a valuable reference for assessing the inflammation ***,the MASA was integrated into a monitoring system using custom *** work provides a promising tool for the stable and reliable monitoring of blood glucose in diabetic patients.

关键词： Microarrow sensor array Glucose sensing Reactive oxygen species sensing Integrated system Continuous monitoring

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Ultra-low frequency magnetic energy focusing for highly effective wireless powering of deep-tissue implantable electronic devices

引用

National Science Review 2024年第05期11卷 272-290页

作者： Yuanyuan Li Zhipeng Chen Yuxin Liu Zijian Liu Tong Wu Yuanxi Zhang Lelun Peng xinshuo Huang shuang Huang Xudong Lin xi xie Lelun Jiang Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument School of Biomedical EngineeringShenzhen Campus of Sun Yat-Sen University School of Mechanical and Electrical Engineering Guangzhou University State Key Laboratory of Optoelectronic Materials and Technologies Guangdong Province Key Laboratory of Display Material and Technology School of Electronics and Information Technology Sun Yat-Sen University

The limited lifespan of batteries is a challenge in the application of implantable electronic devices. Existing wireless power technologies such as ultrasound, near-infrared light and magnetic fields cannot charge devices implanted in deep tissues, resulting in energy attenuation through tissues and thermal ***, an ultra-low frequency magnetic energy focusing(ULFMEF) methodology was developed for the highly effective wireless powering of deep-tissue implantable devices. A portable transmitter was used to output the low-frequency magnetic field(xisting wireless powering technologies. The ULFMEF is demonstrated to wirelessly power implantable micro-LED devices for optogenetic neuromodulation, and wirelessly charged an implantable battery for programmable electrical stimulation on the sciatic nerve. It also bypassed thick and tough protective shells to power the implanted devices. The ULFMEF thus offers a highly advanced methodology for the generation of wireless powered biodevices.

关键词： ultra-low frequency transcutaneous energy transmission electromagnetic generator implantable electronic device deep tissue

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：