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Metabolic profiles and morphological characteristics of leaf tips among different sweet potato(Ipomoea batatas Lam.)varieties

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Journal of Integrative Agriculture 2024年第2期23卷 494-510页

作者： Wenqing Tan xinbo guo Zhangying Wang Rong Zhang Chaochen Tang Bingzhi Jiang Ruixue Jia Yuanyuan Deng Shaohai Yang Jingyi Chen Crops Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Crop Genetic Improvement of Guangdong ProvinceGuangzhou 510640China School of Food Science and Engineering South China University of TechnologyGuangzhou 510640China Sericultural&Agri-Food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional FoodsMinistry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products ProcessingGuangzhou 510610China

Sweet potato leaf tips have high nutritional value,and exploring the differences in the metabolic profiles of leaf tips among different sweet potato varieties can provide information to improve their *** this study,a UPLC-Q-Exactive Orbitrap/MS-based untargeted metabolomics method was used to evaluate the metabolites in leaf tips of 32 sweet potato *** varieties with distinct overall metabolic profiles(A01,A02,and A03),two varieties with distinct profiles of phenolic acids(A20 and A18),and three varieties with distinct profiles of flavonoids(A05,A12,and A16)were *** addition,a total of 163 and 29 differentially expressed metabolites correlated with the color and leaf shape of sweet potato leaf tips,respectively,were identified through morphological *** comparison analysis of the phenotypic traits and a metabolite-phenotypic trait correlation analysis indicated that the color differences of sweet potato leaf tips were markedly associated with ***,the level of polyphenols was correlated with the leaf shape of sweet potato leaf tips,with lobed leaf types having higher levels of polyphenols than the entire leaf *** findings on the metabolic profiles and differentially expressed metabolites associated with the morphology of sweet potato leaf tips can provide useful information for breeding sweet potato varieties with higher nutritional value.

关键词： sweet potato leaf tips phenotypic traits metabolic profile differentially expressed metabolites polyphenols

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基于反步法的下肢外骨骼机器人控制研究

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机电工程 2024年第9期41卷 1692-1703页

作者：苏东海王海啸梁全郭新博王忠伟沈阳工业大学机械工程学院辽宁沈阳110870

针对下肢外骨骼预定轨迹的控制问题,在构建了二自由度机械腿的运动学和动力学模型的基础上,提出了一种非对称液压缸负载力、伸缩量与外骨骼转动扭矩、运动角度的转化关系,采用反步法控制下肢外骨骼系统的运动轨迹。首先,采用D-H法建立... 详细信息

针对下肢外骨骼预定轨迹的控制问题,在构建了二自由度机械腿的运动学和动力学模型的基础上,提出了一种非对称液压缸负载力、伸缩量与外骨骼转动扭矩、运动角度的转化关系,采用反步法控制下肢外骨骼系统的运动轨迹。首先,采用D-H法建立了二自由度下肢外骨骼系统的运动学模型,研究了外骨骼末端执行器关节速度的变化关系,并利用拉格朗日动力学方程推导了外骨骼动力学的数学模型;然后,使用了液压缸负载力控制为机械腿运动提供了相应的扭矩,进而控制了下肢外骨骼的运动姿态;其次,针对下肢外骨骼运动时的高精度要求,利用反步法控制理论,建立了阀控非对称缸系统,结合下肢外骨骼动力学系统整体的状态空间方程,利用下肢外骨骼液压伺服系统控制了下肢外骨骼进行预定轨迹运动;最后,对AMESim软件与Visual Studio软件进行了联合仿真,对比分析了PID控制与反步法控制的精度问题。研究结果表明:针对下肢外骨骼预定轨迹控制,反步法控制误差最大值为2°,对比传统PID控制,误差降低了67%;设计的反步法控制器对于下肢外骨骼具有良好的控制精度。

关键词：电液伺服系统非对称液压缸反步法控制下肢外骨骼系统状态空间方程 D-H法 AMESim 控制精度

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经管类专业人才培养中课程思政建设探究

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漯河职业技术学院学报 2024年第2期23卷 99-103页

作者：胡耘通郭鑫波杜宇森朱立闯西南政法大学法务会计与财税合规研究中心重庆401120

立德树人是教育的根本任务,立德树人的成效是检验高校一切工作的根本标准。在新时代背景下,课程思政对经管类专业人才培养具有重要的意义。面对经管类专业人才培养中存在的教师教学方法陈旧、课程思政体系不完善等问题,亟须加强队伍建设... 详细信息

立德树人是教育的根本任务,立德树人的成效是检验高校一切工作的根本标准。在新时代背景下,课程思政对经管类专业人才培养具有重要的意义。面对经管类专业人才培养中存在的教师教学方法陈旧、课程思政体系不完善等问题,亟须加强队伍建设,提升教师思政水平;深化课程目标,推动理论“本土化”;创新教学方式,拓宽课程思政实现渠道。

关键词：课程思政经管类专业人才培养

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基于蔬菜核酸检测实验的探究性实验教学设计

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实验室科学 2024年第1期27卷 58-61,65页

作者：刘金钏严慧玲郭新波向楠华南理工大学轻工与食品国家级实验教学示范中心食品科学与工程学院广东广州510641

以蔬菜中维生素C积累相关基因的检测为例,探索将操作难度相对较高的RNA相关的核酸检测实验应用于本科实验教学中。实践结果表明,学生们可顺利掌握蔬菜中核糖核酸(RNA)的提取、反转录合成互补DNA(cDNA)、PCR扩增检验目的基因表达等RNA相... 详细信息

以蔬菜中维生素C积累相关基因的检测为例,探索将操作难度相对较高的RNA相关的核酸检测实验应用于本科实验教学中。实践结果表明,学生们可顺利掌握蔬菜中核糖核酸(RNA)的提取、反转录合成互补DNA(cDNA)、PCR扩增检验目的基因表达等RNA相关的实验操作技能,达到了预期的教学目标。本实验项目的实施促进了学生们对多门学科知识的融会贯通,有助于其提高发现问题和解决问题的能力并形成探索精神和创新思维。这对培养具有创新创业实践能力的复合型人才具有重要的作用。

关键词：实验教学 RNA提取反转录PCR 核酸检测

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Genome-wide association study of vitamin E in sweet corn kernels

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The Crop Journal 2020年第2期8卷 341-350页

作者： Yingni Xiao Yongtao Yu Gaoke Li Lihua Xie xinbo guo Jiansheng Li Yuliang Li Jianguang Hu Crop Research Institute Guangdong Academy of Agricultural SciencesKey Laboratory of Crops Genetics and Improvement of Guangdong ProvinceGuangzhou 510640GuangdongChina School of Food Science and Engineering South China University of TechnologyGuangzhou 510641GuangdongChina National Maize Improvement Center of China Beijing Key Laboratory of Crop Genetic ImprovementChina Agricultural UniversityBeijing 100193China

Vitamin E,consisting of tocopherols and tocotrienols,serves as a lipid-soluble antioxidant in sweet corn kernels,providing nutrients to both plants and *** the key genes involved in the vitamin E biosynthesis pathway have been identified in plants,the genetic architecture of vitamin E content in sweet corn kernels remains largely *** the present study,an association panel of 204 inbred lines of sweet corn was *** compounds of vitamin E were quantified in sweet corn kernels at 28 days after pollination.A total of 119 loci for vitamin E were identified using a genome-wide association study based on genotyping by sequencing,and a genetic network of vitamin E was *** genes identified were involved mainly in RNA regulation and protein *** known gene ZmVTE4,encodingγ-tocopherol methyltransferase,was significantly associated with four traits(α-tocopherol,α-tocotrienol,theα/γ-tocopherol ratio,and theα/γ-tocotrienol ratio).The effects of two causative markers on ZmVTE4 were validated by haplotype ***,two elite cultivars(Yuetian 9 and Yuetian 22)with a 4.5-fold increase in the sum ofα-andγ-tocopherols were developed by marker-assisted selection,demonstrating the successful biofortification of sweet *** genes(DAHPS,ADT2,and cmu2)involved in chorismate and tyrosine synthesis were significantly associated with theα/γ-tocotrienol *** results shed light on the genetic architecture of vitamin E and may accelerate the nutritional improvement of sweet corn.

关键词： Sweet corn GBS Genome-wide association study Vitamin E Marker-assisted selection

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A universal strategy of multi-objective active learning to accelerate the discovery of organic electrode molecules

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Science China Chemistry 2024年第11期67卷 3681-3687页

作者： Jiayi Du Jun guo Wei Liu Ziwei Li Gang Huang xinbo Zhang State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences School of Applied Chemistry and Engineering University of Science and Technology of China School of Materials Science and Engineering Changchun University of Science and Technology

Organic electrode molecules hold significant potential as the next generation of cathode materials for Li-ion batteries. In this study, we have introduced a multi-objective active learning framework that leverages Bayesian optimization and non-dominated sorting genetic algorithms-Ⅱ. This framework enables the selection of organic molecules characterized by high theoretical energy density and low gap(LUMO-HOMO)(LUMO, lowest unoccupied molecular orbital; HOMO, highest occupied molecular orbital). Remarkably, after only two cycles of active learning, the determination of coefficient can reach 0.962 for theoretical energy density and 0.920 for the gap with a modest dataset of 300 molecules, showcasing superior predictive capabilities. The 2,3,5,6-tetrafluorocyclohexa-2,5-diene-1,4-dione, selected by non-dominated sorting genetic algorithms-Ⅱ, has been successfully applied to Li-ion batteries as cathode materials, demonstrating a high capacity of 288 m Ah g-1and a long cycle life of 1,000 cycles. This outcome underscores the high reliability of our framework. Furthermore, we have also validated the universality and transferability of our framework by applying it to two additional databases, the QM9 and OMEAD. When the training dataset of the model includes at least 500 molecules, the determination of coefficient essentially reaches approximately0.900 for four targets: gap, reduction potential, LUMO, and HOMO. Therefore, the universal framework in our work provides innovative insights applicable to other domains to expedite the screening process for target materials.

关键词： organic electrode molecules Li-ion batteries active learning multi-objective Bayesian optimization

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Comparison of fatty acid composition,phytochemical profile and antioxidant activity in four flax(Linum usitatissimum L.)varieties

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Oil Crop Science 2020年第3期5卷 136-141页

作者： Caisheng Qiu Hong Wang Yuan guo Songhua Long Yufu Wang Arshad Mehmood Abbasi xinbo guo Devra I.Jarvis Institute of Bast Fiber Crops Chinese Academy of Agricultural SciencesChangsha410205China School of Food Science and Engineering South China University of TechnologyGuangzhou510640China Department of Environment Sciences COMSATS University IslamabadAbbottabad Campus22060Pakistan Platform for Agrobiodiversity Research C/o Alliance of Bioversity International and CIAT Via Dei Tre Denari 472/a00057MaccareseRomeItaly Department of Crop and Soil Sciences Washington State UniversityPullmanWAUSA

The present study evaluates variations among flaxseed varities in terms of fatty acid composition,phytochemical profile,and antioxidant activity determined by oxygen radical absorbance capacity(ORAC),2,2-Diphenyl-1-picrylhydrazyl(DPPH)and ferrous ion reducing antioxidant power(FRAP)*** variations in fatty acid composition,phenolic acids and lignan were observed in flaxseed varieties from different *** them,unsaturated fatty acids accounted over 4/5 of total fatty acid *** highest ratio of linolenic acid of total fatty acid was observed in USPEA,whereas the lowest one was found in *** showed the highest content of total phenolics,as well as flaxseed *** general,total phenolics appeared to be the main contributors of antioxidant capacity of flaxseed,which presented significant positive *** study revealed that both cultivar and origin of seed significantly affected fatty acid composition,phenolic acids,lignans and subsequent antioxidant activity of *** results provided new aspects of breeding resources of flaxseed cultivars by presenting their quality specification and possible commercial value.

关键词： Flaxseed Phytochemicals Lignan Fatty acid Antioxidant activity

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Biophysical-driven piezoelectric and aligned nanofibrous scaffold promotes bone regeneration by re-establishing physiological electrical microenvironment

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Nano Research 2024年第8期17卷 7376-7393页

作者： Aoao Wang xinbo Ma Yafeng Yang guoliang Shi Liwei Han Xiantong Hu Rui Shi Jun Yan Quanyi guo Yantao Zhao Senior Department of Orthopedics the Fourth Medical Center of PLA General HospitalBeijing 100048China Department of Chemistry Capital Normal UniversityBeijing 100048China National Center for Orthopaedics Beijing Research Institute of Traumatology and OrthopaedicsBeijing Jishuitan HospitalCapital Medical UniversityBeijing 100035China Xijing 986 Hospital Department The Fourth Military Medical UniversityXi'an 710032China Institute of Orthopedics Chinese PLA General HospitalBeijing Key Laboratory of Regenerative Medicine in OrthopedicsKey Laboratory of Musculoskeletal Trauma&War Injuries PLABeijing 100853China

The initial healing stages of bone fracture is a complex physiological process involving a series of spatially and temporally overlapping events,including pathogen clearance,immunological modulation,and *** this study,we have developed a piezoelectric and aligned nanofibrous scaffold composed of ZnO@PCL/PVDF with multiple antibacterial,immunomodulatory,and osteogenic effects using electrospinning *** scaffold’s piezoelectric signal output under ultrasound(US)control can be similar to the physiological electrical signals of healthy bone tissue,creating a truly biomimetic electrical microenvironment in the bone *** vitro studies have shown that ZnO@PCL/PVDF scaffold significantly enhances the proliferation,migration,and osteogenic differentiation of MC3T3-E1 cells under piezoelectric drive provided by ***,the scaffold exhibits inhibitory effects on the growth of *** and ***,as well as the ability to induce M2 macrophage polarization,indicating potent antibacterial and immunomodulatory *** vivo experiments demonstrated that the ZnO@PCL/PVDF scaffold can accelerate the repair of mandibular defects in rats,effectively inhibit bacterial colonization,and reduce inflammatory ***,this study confirms that the newly developed ZnO@PCL/PVDF scaffold effectively promotes bone repair by truly mimicking the endogenous electrical microenvironment and precisely regulating the temporospatial disorders of initial bone healing,thus providing a simple and effective solution for bone defects.

关键词： piezoelectricity bone defect bacterial infection ZnO nanoparticles immunomodulation osteogenesis

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Innovations in metallurgical and materials processes:advancing liquid theory and honoring ***-jie guo's contributions to physical chemistry in metallurgy

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Journal of Iron and Steel Research International 2024年第1期31卷 1-2页

作者： Shuai Liu Jing guo Han-jie guo School of Artificial Intelligence Beijing Technology and Business UniversityBeijing100048China School of Metallurgical and Ecological Engineering University of Science and Technology Beijing(USTB)Beijing100083China

Spanning three decades,***-jie guo's research group has dedicated itself to exploring the fundamental theories and practical applications of physical chemistry within the realm of *** under the assumption that molecul... 详细信息

关键词： chemistry liquid complexes

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Image Analysis of the Sugar-reduced Muffin Formulated with Stevianna or Inulin as a Sugar Replacer

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Grain & Oil Science and Technology 2018年第2期1卷 63-71页

作者： GAO Jingrong HAN Fezhong guo xinbo ZENG Xi'an MASON Susan L BRENNAN Margaret A BRENNAN Charles S Department of Wine Food and Molecular Biosciences Lincoln University Riddet Institute College of Light Industry and Food Science South China University of Technology

Sugar replacement, and/or reduction, in cereal products is important in relation to consumer appeal for nutritionally balanced, calorie reduced, products. However, when using sugar replacers, product structure can be negatively altered. The effect on air cell development and microstructure with the use of Stevianna, or inulin, as sugar replacer was evaluated using muffins as a model food. The total replacement of sugar using Stevianna or inulin resulted in the development of non-uniform air cells leading to poor muffin microstructure. However, formulations involving partial sugar replacers gave similar air cell characteristics and hence microstructural characteristics, to that of the control sample. The results indicated that sugar is an important factor for the development of muffin microstructure and is difficult to replace completely.

关键词： Stevianna Inulin Muffin microstructure Air cell Scanning electron microscopy

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