检索结果-南通市图书馆

Establishment of a Multiplex Detection Method for Common Bacteria in Blood Based on Human Mannan-Binding Lectin Protein-Conjugated Magnetic Bead Enrichment Combined with Recombinase-Aided PCR Technology

引用

Biomedical and Environmental Sciences 2024年第4期37卷 387-398页

作者： ZHAO Zi Jin CHEN Xiao Ping HUA Shao Wei LI Feng Yu ZHAO Meng xinG Chen Hao wang Jie TIAN Feng Yu ZHANG Rui Qing LYU Xiao Na HAN zhi Qiang wang Yu xin LI Hong Yi SHEN xin xin MA Xue Jun TIE Yan Qing Graduate School Hebei North UniversityZhangjiakou 075000HebeiChina Department of Clinical Laboratory Hebei General HospitalShijiazhuang 050051HebeiChina National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases NHC Key Laboratory of Medical Virology and Viral DiseasesNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China Department of Clinical Laboratory Pathology Diagnostic Center Shiyan General HospitalShiyan 442000HubeiChina Graduate School Hebei Medical University Shijiazhuang 050031HebeiChina

Objective Recombinase-aided polymerase chain reaction(RAP)is a sensitive,single-tube,two-stage nucleic acid amplification *** study aimed to develop an assay that can be used for the early diagnosis of three types of bacteremia caused by Staphylococcus aureus(SA),Pseudomonas aeruginosa(PA),and Acinetobacter baumannii(AB)in the bloodstream based on recombinant human mannanbinding lectin protein(M1 protein)-conjugated magnetic bead(M1 bead)enrichment of pathogens combined with *** Recombinant plasmids were used to evaluate the assay *** blood influenza bacteria were used for the specific *** and clinical plasma samples were enriched with M1 beads and then subjected to multiple recombinase-aided PCR(M-RAP)and quantitative PCR(qPCR)*** analysis was used to evaluate the consistency between the two *** The M-RAP method had sensitivity rates of 1,10,and 1 copies/μL for the detection of SA,PA,and AB plasmids,respectively,without cross-reaction to other bacterial *** M-RAP assay obtained results for<10 CFU/mL pathogens in the blood within 4 h,with higher sensitivity than qPCR.M-RAP and qPCR for SA,PA,and AB yielded Kappa values of 0.839,0.815,and 0.856,respectively(P<0.05).Conclusion An M-RAP assay for SA,PA,and AB in blood samples utilizing M1 bead enrichment has been developed and can be potentially used for the early detection of bacteremia.

关键词： Staphylococcus aureus Pseudomonas aeruginosa Acinetobacter baumannii Human Mannan-binding lectin protein Bloodstream infection Recombinase-aided PCR assay Multiple detection

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Hydrodynamic Performance of An Integrated System of Breakwater and A Multi-Chamber OWC Wave Energy Converter

引用

China Ocean Engineering 2024年第4期38卷 543-556页

作者： NING De-zhi ZHANG Xiang-yu wang Rong-quan ZHAO Ming State Key Laboratory of Coastal and Offshore Engineering Dalian University of TechnologyDalian 116024China Dalian Key Laboratory of Offshore Renewable Energy Dalian University of TechnologyDalian 116024China School of Computing Engineering and MathematicsUniversity of Western SydneyPenrith NSW 2751Australia

A multi-chamber oscillating water column wave energy converter(OWC-WEC)integrated to a breakwater is *** hydrodynamic characteristics of the device are analyzed using an analytical model based on the linear potential flow theory.A pneumatic model is employed to investigate the relationship between the air mass flux in the chamber and the turbine *** effects of chamber width,wall draft and wall thickness on the hydrodynamic performance of a dual-chamber OWC-WEC are *** results demonstrate that the device,with a smaller front wall draft and a wider rear chamber exhibits a broader effective frequency *** device with a chamber-width-ratio of 1:3 performs better in terms of power ***,results from the analysis of a triplechamber OWC-WEC demonstrate that reducing the front chamber width and increasing the rearward chamber width can improve the total performance of the *** the number of chambers from 1 to 2 or 3 can widen the effective frequency bandwidth.

关键词： oscillating water column power extraction efficiency potential flow theory wave energy converter multi-chamber

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Advances and clinical applications of immune checkpoint inhibitors in hematological malignancies

引用

Cancer Communications 2024年第9期44卷 1071-1097页

作者： Wenyue Sun Shunfeng Hu xin wang Department of Hematology Shandong Provincial HospitalShandong UniversityJinanShandongP.R.China Department of Hematology Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinanShandongP.R.China Taishan Scholars Program of Shandong Province JinanShandongP.R.China Branch of National Clinical Research Center for Hematologic Diseases JinanShandongP.R.China National Clinical Research Center for Hematologic Diseases the First Affiliated Hospital of Soochow UniversitySuzhouJiangsuP.R.China

Immune checkpoints are differentially expressed on various immune cells to regulate immune responses in tumor *** cells can activate the immune checkpoint pathway to establish an immunosuppressive tumor microenvironment and inhibit the anti-tumor immune response,which may lead to tumor progression by evading immune *** coinhibitory signaling pathways with immune checkpoint inhibitors(ICIs)could reinvigorate the anti-tumor immune response and promote immune-mediated eradication of tumor *** a milestone in tumor treatment,ICIs have been firstly used in solid tumors and subsequently expanded to hematological malignancies,which are in their ***,immune checkpoints have been investigated as promising biomarkers and therapeutic targets in hematological malignancies,and novel immune checkpoints,such as signal regulatory proteinα(SIRPα)and tumor necrosis factor-alpha-inducible protein 8-like 2(TIPE2),are constantly being *** ICIs have received clinical approval for clinical application in the treatment of hematological malignancies,especially when used in combination with other strategies,including oncolytic viruses(OVs),neoantigen vaccines,bispecific antibodies(bsAb),bio-nanomaterials,tumor vaccines,and cytokine-induced killer(CIK)***,the proportion of individuals with hematological malignancies benefiting from ICIs remains lower than expected due to multiple mechanisms of drug resistance and immune-related adverse events(irAEs).Close monitoring and appropriate intervention are needed to mitigate irAEs while using *** review provided a comprehensive overview of immune checkpoints on different immune cells,the latest advances of ICIs and highlighted the clinical applications of immune checkpoints in hematological malignancies,including biomarkers,targets,combination of ICIs with other therapies,mechanisms of resistance to ICIs,and irAEs,which can provide novel insight into the future

关键词： Immune checkpoint hematological malignancies biomarkers therapeutic targets drug resistance

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Progress in manipulating spin polarization for solar hydrogen production

引用

Materials Reports(Energy) 2024年第1期4卷 43-57页

作者： Qian Yang xin Tong zhiming wang Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of ChinaChengdu 610054PR China Yangtze Delta Region Institute(Huzhou) University of Electronic Science and Technology of ChinaHuzhou 313001PR China Institute for Advanced Study Chengdu UniversityChengdu 610106PR China

Photocatalytic and photoelectrochemical water splitting using semiconductor materials are effective approaches for converting solar energy into hydrogen *** the past few years,a series of photocatalysts/photoelectrocatalysts have been developed and optimized to achieve efficient solar hydrogen *** various optimization strategies,the regulation of spin polarization can tailor the intrinsic optoelectronic properties for retarding charge recombination and enhancing surface reactions,thus improving the solar-to-hydrogen(STH)*** review presents recent advances in the regulation of spin polarization to enhance spin polarized-dependent solar hydrogen evolution ***,spin polarization manipulation strategies of several typical photocatalysts/photoelectrocatalysts(e.g.,metallic oxides,metallic sulfides,non-metallic semiconductors,ferroelectric materials,and chiral molecules)are *** the end,the critical challenges and perspectives of spin polarization regulation towards future solar energy conversion are briefly provided.

关键词： Spin polarization Solar energy conversion Photocatalytic hydrogen production Photoelectrochemical water splitting

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Causal association between 25-hydroxyvitamin D status and cataract development:A two-sample Mendelian randomization study

引用

World Journal of Clinical Cases 2024年第16期12卷 2789-2795页

作者： Chun-Hui wang zhi-Kun xin Department of Ophthalmology Aviation General HospitalBeijing 100010China

BACKGROUND Vitamin deficiencies are linked to various eye diseases,and the influence of vitamin D on cataract formation has been noted in prior ***,detailed investigations into the causal relationship between 25-(OH)D status and cataract development remain *** To explore a possible causal link between cataracts and vitamin *** In this study,we explored the causal link between 25-(OH)D levels and cataract development using Mendelian *** analytical approach included inverse-variance weighting(IVW),MR-Egger,weighted median,simple mode,and weighted mode *** primary analyses utilized IVW with random effects,supplemented by sensitivity and heterogeneity tests using both IVW and ***-Egger was also applied for pleiotropy ***,a leave-one-out analysis helped identify potentially impactful single-nucleotide *** The analysis revealed a positive association between 25-(OH)D levels and the risk of developing cataracts(OR=1.11,95%CI:1.00-1.22;P=0.032).The heterogeneity test revealed that our IVW analysis exhibited minimal heterogeneity(P>0.05),and the pleiotropy test findings confirmed the absence of pleiotropy within our IVW analysis(P>0.05).Furthermore,a search of the human genotype-phenotype association database failed to identify any potentially relevant risk-factor single nucleotide *** There is a potential causal link between 25-(OH)D levels and the development of cataracts,suggesting that greater 25-(OH)D levels may be a contributing risk factor for cataract *** experimental research is required to confirm these findings.

关键词： Cataract 25-hydroxyvitamin D Mendelian randomization Single nucleotide polymorphism Causal relationship

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Adaptive security control of time-varying constraints nonlinear cyber-physical systems with false data injection attacks

引用

Journal of Control and Decision 2024年第1期11卷 50-59页

作者： Yue-Ming wang Yuan-xin Li Department of Science Liaoning University of TechnologyJinzhouPeople’s Republic of China

In this article,an adaptive security control scheme is presented for cyber-physical systems(CPSs)suffering from false data injection(FDI)attacks and time-varying state ***,an adaptive bound estimation mechanism is introduced in the backstepping control design to mitigate the effect of FDI ***,to solve the unknown sign time-varying statefeedback gains aroused by the FDI attacks,a type of Nussbaum function is employed in the adaptive security ***,by constructing a barrier Lyapunov function,it can be ensured that all signals of controlled system are bounded and the time-varying state constraints are not ***,the provided simulation examples demonstrate the effectiveness of the proposed controller.

关键词： Neural networks backstepping technology false data injection(FDI)attacks nonlinear cyber-physical systems controls

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of Mo and cold forging deformation on strength and ductility of cobalt-based alloy L605

引用

Journal of Iron and Steel Research International 2024年第7期31卷 1811-1823页

作者： Zhong-lin wang zhi-hua Gong Quan Li Han-sheng Bao School of Materials and Metallurgy Inner Mongolia University of Science and TechnologyBaotou 014017Inner MongoliaChina Institute of Special Steels Central Iron and Steel Research Institute Co.Ltd.Beijing 100081China

Mo element was added to cobalt-based alloy L605,and cold forging deformation was *** effects of the addition and cold forging deformation on the microstructure and mechanical properties of the alloy were studied by thermodynamic calculation,electron backscatter diffraction,transmission electron microscopy,and X-ray *** stacking fault energy(SFE)of the alloy decreased after the addition,and the formation of stacking faults and intersections were promoted to improve the strength and *** tensile strength of the alloy with Mo increased from 1190 to 1702 MPa after 24%cold deformation,producing significant work *** strengthening mechanism is strain-induced martensitic transformation(SIMT)and deformation *** alloy,combined with Mo and after 24%deformation,had both high strength and ductility in comparison with the original cobalt-based alloy *** is attributed to the lower SFE which caused the increase in stacking fault *** the tensile process,theε-hcp phase was easily generated at the stacking fault to reduce the stress concentration and increase the *** SIMT by adjusting the density of stacking faults can improve the mechanical properties of cobalt-based ***ε-hcp phase,the interaction between deformation twins and dislocations,and the interaction between e-hcp phases during cold forging deformation caused local stress concentration,lowering ductility and toughness.

关键词： Cobalt-based alloy-Stacking fault energy Strain-induced martensitic transformation Deformation twin Cold forging deformation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The regulatory roles and clinical significance of glycolysis in tumor

引用

Cancer Communications 2024年第7期44卷 761-786页

作者： Qiqi Qiao Shunfeng Hu xin wang Department of Hematology Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinanShandongP.R.China Department of Hematology Shandong Provincial HospitalShandong UniversityJinanShandongP.R.China Taishan Scholars Program of Shandong Province JinanShandongP.R.China Branch of National Clinical Research Center for Hematologic Diseases JinanShandongP.R.China National Clinical Research Center for Hematologic Diseases the First Affiliated Hospital of Soochow UniversitySuzhouJiangsuP.R.China

Metabolic reprogramming has been demonstrated to have a significant impact on the biological behaviors of tumor cells,among which glycolysis is an important *** research has revealed that the heightened glycolysis levels,the abnormal expression of glycolytic enzymes,and the accumulation of glycolytic products could regulate the growth,proliferation,invasion,and metastasis of tumor cells and provide a favorable microenvironment for tumor development and *** on the distinctive glycolytic characteristics of tumor cells,novel imaging tests have been developed to evaluate tumor proliferation and *** addition,glycolytic enzymes have been found to serve as promising biomarkers in tumor,which could provide assistance in the early diagnosis and prognostic assessment of tumor *** glycolytic enzymes have been identified as potential therapeutic targets for tumor treatment,and various small molecule inhibitors targeting glycolytic enzymes have been developed to inhibit tumor development and some of them are already applied in the *** this review,we systematically summarized recent advances of the regulatory roles of glycolysis in tumor progression and highlighted the potential clinical significance of glycolytic enzymes and products as novel biomarkers and therapeutic targets in tumor treatment.

关键词： glycolysis tumor development biomarkers targeted therapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Learning to Branch in Combinatorial Optimization With Graph Pointer Networks

引用

IEEE/CAA Journal of Automatica Sinica 2024年第1期11卷 157-169页

作者： Rui wang zhiming Zhou Kaiwen Li Tao Zhang Ling wang xin Xu Xiangke Liao IEEE the Xiangjiang Laboratory and the College of Systems Engineering National University of Defense TechnologyChangsha 410073China the Institute of Automation Chinese Academy of SciencesBeijing 100190China the College of Systems Engineering National University of Defense TechnologyChangsha 410073 the Hunan Key Laboratory of Multi-Energy System Intelligent Interconnection Technology Changsha 410073China the Department of Automation Tsinghua UniversityBeijing 100084China the College of Intelligence Science and Technology National University of Defense TechnologyChangsha 410073China the College of Computer Science and Technology National University of Defense TechnologyChangsha 410073China

Traditional expert-designed branching rules in branch-and-bound(B&B) are static, often failing to adapt to diverse and evolving problem instances. Crafting these rules is labor-intensive, and may not scale well with complex *** the frequent need to solve varied combinatorial optimization problems, leveraging statistical learning to auto-tune B&B algorithms for specific problem classes becomes attractive. This paper proposes a graph pointer network model to learn the branch rules. Graph features, global features and historical features are designated to represent the solver state. The graph neural network processes graph features, while the pointer mechanism assimilates the global and historical features to finally determine the variable on which to branch. The model is trained to imitate the expert strong branching rule by a tailored top-k Kullback-Leibler divergence loss function. Experiments on a series of benchmark problems demonstrate that the proposed approach significantly outperforms the widely used expert-designed branching rules. It also outperforms state-of-the-art machine-learning-based branch-and-bound methods in terms of solving speed and search tree size on all the test instances. In addition, the model can generalize to unseen instances and scale to larger instances.

关键词： Branch-and-bound(B&B) combinatorial optimization deep learning graph neural network imitation learning

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

12.6μm-Thick Asymmetric Composite Electrolyte with Superior Interfacial Stability for Solid-State Lithium-Metal Batteries

引用

Nano-Micro Letters 2024年第9期16卷 397-409页

作者： Zheng Zhang Jingren Gou Kaixuan Cui xin Zhang Yujian Yao Suqing wang Haihui wang Beijing Key Laboratory for Membrane Materials and Engineering Department of Chemical EngineeringTsinghua UniversityBeijing 100084People’s Republic of China School of Chemistry and Chemical Engineering South China University of TechnologyGuangzhou 510000People’s Republic of China

Solid-state lithium metal batteries(SSLMBs)show great promise in terms of high-energy-density and high-safety ***,there is an urgent need to address the compatibility of electrolytes with high-voltage cathodes/Li anodes,and to minimize the electrolyte thickness to achieve highenergy-density of ***,we develop an ultrathin(12.6μm)asymmetric composite solid-state electrolyte with ultralight areal density(1.69 mg cm^(−2))for *** electrolyte combining a garnet(LLZO)layer and a metal organic framework(MOF)layer,which are fabricated on both sides of the polyethylene(PE)separator separately by tape *** PE separator endows the electrolyte with flexibility and excellent mechanical *** LLZO layer on the cathode side ensures high chemical stability at high *** MOF layer on the anode side achieves a stable electric field and uniform Li flux,thus promoting uniform Li^(+)*** to the well-designed structure,the Li symmetric battery exhibits an ultralong cycle life(5000 h),and high-voltage SSLMBs achieve stable cycle *** assembled pouch cells provided a gravimetric/volume energy density of 344.0 Wh kg^(−1)/773.1 Wh L^(−1).This simple operation allows for large-scale preparation,and the design concept of ultrathin asymmetric structure also reveals the future development direction of SSLMBs.

关键词： Solid-state lithium metal batteries Composite solid-state electrolyte Ultrathin asymmetric structure Pouch cells

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：