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Engineering organoid microfluidic system for biomedical and health engineering:A review

Chinese Journal of Chemical Engineering 2021年第2期34卷 244-254页

作者： Yifan xing Junyu Liu Xiaojie Guo Haipeng Liu Wen zeng Yi Wang Chong Zhang Yuan Lu Dong He Shaohua Ma Yonghong He xin-Hui xing Key Laboratory for Industrial Biocatalysis Ministry of Education of ChinaDepartment of Chemical EngineeringTsinghua UniversityBeijing 100084China Centre for Synthetic and Systems Biology Tsinghua UniversityBeijing 100084China School of Chemical Engineering and Light Industry Guangdong University of TechnologyGuangzhou 510006China Institute of Biopharmaceutical and Health Engineering Tsinghua Shenzhen International Graduate SchoolShenzhen 518055China Institute of Biomedical Health Technology and Engineering Shenzhen Bay LaboratoryShenzhen 518055China

In recent years,organoid technology,i.e.,in vitro three-dimensional(3D)tissue culture,has attracted increasing attention in biomedical *** are cell complexes induced by differentiation of stem cells or organ-progenitor cells in vitro using 3D culture *** can replicate the key structural and functional characteristics of the target organs in *** the opening up of this new field of health engineering,there is a need for engineering-system approaches to the production,control,and quantitative analysis of organoids and their *** organoid technology has limitations,including lack of physical and chemical microenvironment control,high heterogeneity,complex manual operation,imperfect nutritional supply system,and lack of feasible online analytical technology for the *** introduction of microfluidic chip technology into organoids has overcome many of these limitations and greatly expanded the scope of *** organoid microfluidic system has become an interdisciplinary field in biomedical and health *** this review,we summarize the development and culture system of organoids,discuss how microfluidic technology has been used to solve the main technical challenges in organoid research and development,and point out new opportunities and prospects for applications of organoid microfluidic system in drug development and screening,food safety,precision medicine,and other biomedical and health engineering fields.

关键词： Organoids Stem cell Culture system Microfluidics Biomedical engineering Human health

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DMF-scMT-seq linking methylome and transcriptome within single cells with digital microfluidics

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Science China Chemistry 2024年第6期67卷 2070-2078页

作者： xing Xu Xi zeng xin Lin Shiyan Lin Shanshan Liang Tian Tian Rui Su Jia Song Chaoyong Yang Collaborative Innovation Center of Chemistry for Energy Materials The MOE Key Laboratory of Spectrochemical Analysis and InstrumentationState Key Laboratory of Physical Chemistry of Solid SurfacesDepartment of Chemical BiologyCollege of Chemistry and Chemical EngineeringThe First Affiliated Hospital of Xiamen UniversityXiamen UniversityXiamen361005China Institute of Molecular Medicine Renji HospitalShanghai Jiao Tong University School of MedicineShanghai200127China Chemistry and Materials Science College Shanghai Normal UniversityShanghai200234China Chemistry and Biomedicine Innovation Center(ChemBIC) School of Chemistry and Chemical EngineeringNanjing UniversityNanjing210023China

Single-cell joint analysis of methylome and transcriptome reveals how the methylation regulates the transcriptional ***,traditional bench-top protocols for single-cell DNA methylation and RNA transcription co-detection are laborintensive,cost-ineffective and ***,we establish the DMF-sc MT-seq,a highly-efficient and cost-effective method to simultaneously analyze single-cell DNA methylation and transcriptional activity based on digital *** MT-seq automates the workflow of single-cell isolation,cellular hypotonic lysis,nucleic acid separation and methylome/transcriptome library construction in a contactless and addressable *** system ensures high accuracy(R>0.85),high gene detection ability(14,697 genes per cell at 4 million sequencing depth),and high CpG coverage(677,198 CpG sites per cell at 1million sequencing depth).By using DMF-sc MT-seq,the relationship of DNA methylation and RNA transcription under different genomic contexts is *** further apply DMF-sc MT-seq to study the dynamics of transcription regulation with methylation-inhibiting anti-tumor Decitabine,and identify the methylated promoter/gene body driven genes in response to Decitabine ***-sc MT-seq facilitates the construction of the correlation of DNA methylation and transcriptional activity at the single-cell level in a flexible,sensitive and accurate way,which is anticipated to be a powerful tool in studying single-cell biological systems.

关键词： single-cell analysis digital microfluidics methylome sequencing transcriptome sequencing

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Study on concentration distribution and detonation characteristics for non-axisymmetric fuel dispersal

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Defence Technology（防务技术） 2024年第1期31卷 484-495页

作者： Linghui zeng Zhongqi Wang xing Chen Jianping Li State Key Laboratory of Explosion Science and Technology Beijing Institute of TechnologyBeijing100081China College of Environment and Chemical Engineering Sichuan University of Science&EngineeringZigong643000China

The study of non-axisymmetric fuel dispersal and detonation can provide reference for the prevention of industrial cloud explosion accidents and the design of fuel air explosive(FAE).The concentration and detonation fields of 85 kg cylindrical and fan-shaped fuel are investigated by experiments and numerical simulations.A dynamic model of the whole process for fuel dispersal and detonation is *** concentration distribution of fuel is used as the initial condition to calculate the detonation stage,thus solving the initial value problem of detonation *** phase and component changes of fuel cloud at different locations are *** fuel cloud is divided into directions of 0°,90°,135°and 180°.The results show that the maximum cloud radius is 20.94 m in 135°and the minimum is 12.04 m in 0°.The diameter of the detonation fireball is 53.6 m,and the peak temperature is 3455 *** highest peak overpressure is 3.44 MPa in 0°and the lowest is 2.97 MPa in 135°.The proportion of liquid phase in 0°is22.90%,and the fuel loss is 11.8% and 9% higher than that in 135°and cylindrical charge,*** stable propagation distance of blast wave in 135°is 42.50% longer than 0°and 28.37% longer than cylindrical charge.

关键词： Fuel dispersal Concentration distribution Detonation characteristic Fuel loss Numerical simulation

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Multi-Objective Cooperative Salvo Attack Against Group Target

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Journal of Systems Science & Complexity 2018年第1期31卷 244-261页

作者： zeng Jie DOU Lihua xin Bin School of Automation State Key Laboratory of Intelligent Control and Decision of Complex SystemsBeijing Advanced Innovation Center for Intelligent Robots and Systems Beijing Institute of Technology

This paper proposes a solution for the problem of cooperative salvo attack of multiple cruise missiles against targets in a group. Synchronization of the arrival time of missiles to hit their common target, minimizing the time consumption of attack and maximizing the expected damage to group targets are taken into consideration simultaneously. These operational objectives result in a hierarchical mixed-variable optimization problem which includes two types of subproblems, namely the multi-objective missile-target assignment（MOMTA） problem at the upper level and the time-optimal coordinated path planning（TOCPP） problems at the lower level. In order to solve the challenging problem, a recently proposed coordinated path planning method is employed to solve the TOCPP problems to achieve the soonest salvo attack against each target. With the aim of finding a more competent solver for MOMTA, three state-of-the-art multi-objective optimization methods（MOMs）,namely NSGA-II, MOEA/D and DMOEA-εC, are adopted. Finally, a typical example is used to demonstrate the advantage of the proposed method. A simple rule-based method is also employed for comparison. Comparative results show that DMOEA-εC is the best choice among the three MOMs for solving the MOMTA problem. The combination of DMOEA-εC for MOMTA and the coordinated path planning method for TOCPP can generate obviously better salvo attack schemes than the rule-based method.

关键词： Coordinated path planning missile-target assignment multi-missile salvo attack multiobjective optimization

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Advanced regeneration and fixed-bed study of ammonium and potassium removal from anaerobic digested wastewater by natural zeolite

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Journal of Environmental Sciences 2013年第5期25卷 954-961页

作者： Xuejun Guo Larry zeng xin Jin State Key Laboratory of Water Environment Simulation School of EnvironmentBeijing Normal University Environmental Technologies Alberta Research Council Inc.VegrevilleAlberta T9C 1T4Canada

Highly efficient regeneration of natural zeolite was developed in conjunction with the removal of high concentrations of ammonia and potassium from the reverse osmosis effluent of anaerobic-digested wastewater by fixed-bed ion *** elution and uptake behavior of ammonium and potassium in the fixed bed were *** batch desorption tests and on-column regeneration were conducted to develop an optimum regeneration condition compatible with the wastewater *** effectiveness of ammonium elution increased with increasing alkaline *** increase of salt dose significantly enhanced the ammonium maximum in the elution *** ammonium elution was achieved in 6 bed volumes（BV） when the alkaline and salt concentrations were respectively 0.1 mol/L and 18.6 g/L at a flow rate of 2.5-3.0 BV/*** to the higher affinity of potassium with natural clinoptilolite,complete potassium elution was not achieved in all cases.

关键词： regeneration removal ammonium potassium natural zeolite

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A joint mid-course and terminal course cooperative guidance law for multi-missile salvo attack

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Chinese Journal of Aeronautics 2018年第6期31卷 1311-1326页

作者： Jie zeng Lihua DOU Bin xin School of Automation Beijing Institute of Technology Beijing 100081 China State Key Laboratory of Intelligent Control and Decision of-Complex Systems Beijing 100081 China

Salvo attacking a surface target by multiple missiles is an effective tactic to enhance the lethality and penetrate the defense system. However, existing cooperative guidance laws in the midcourse or terminal course are not suitable for long-and medium-range missiles or stand-off attacking. Because the initial conditions of cooperative terminal guidance that are generally generated from the mid-course flight may not lead to a successful cooperative terminal guidance without proper mid-course flight adjustment. Meanwhile, cooperative guidance in the mid-course cannot solely guarantee the accuracy of a simultaneous arrival of multiple missiles. Therefore, a joint mid-course and terminal course cooperative guidance law is developed. By building a distinct leader-follower framework, this paper proposes an efficient coordinated Dubins path planning method to synchronize the arrival time of all engaged missiles in the mid-course flight. The planned flight can generate proper initial conditions for cooperative terminal guidance, and also benefit an earliest simultaneous arrival. In the terminal course, an existing cooperative proportional navigation guidance law guides all the engaged missiles to arrive at a target accurately and *** integrated guidance law for an intuitive application is summarized. Simulations demonstrate that the proposed method can generate fast and accurate salvo attack.

关键词： Cooperative systems Dubins path Mid-course flight Missile guidance Salvo attack

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Design and evaluation of control strategies in urban drainage systems in Kunming city

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Frontiers of Environmental Science & Engineering 2017年第4期11卷 105-112页

作者： xin Dong Senchen Huang Siyu zeng School of Environment Tsinghua University Beijing 100084 China

Real time control （RTC） of urban drainage systems （UDSs） is an important measure to reduce combined sewer overflow （CSO） and urban flooding, helping achieve the aims of ＂Sponge City＇. Application of RTC requires three main steps： strategy design, simulation-based evaluation and field test. But many of published RTC studies are system-specific, lacking discussions on how to design a strategy step by step. In addition, the existing studies are prone to use hydrologic model to evaluated strategics, but a more precise and dynamic insight into strategy performance is needed. To fill these knowledge gaps, based on a case UDS in Kunming city, a studio on RTC strategy design and Management Model （SWMM） - uncier four typical rainfall events characterized by different return periods （1-year or 0.5 year） and different spatial distributions （uniform or uneven）. The equal filling strategy outperformss other two strategies and it can achieve 10% more CSO reduction and 5% more flooding reduction relative to the no-tank case.

关键词： Urban drainage system Real time control Static strategy Constant strategy Equal filling strategy

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Preparation and application of microfluidic SERS substrate:Challenges and future perspectives

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Journal of Materials Science & Technology 2020年第2期37卷 96-103页

作者： Jiuchuan Guo Fanyu zeng Jinhong Guo xing Ma School of Information and Communication Engineering University of Electronic Science and Technology of ChinaChengdu 610054China State Key Laboratory of Advanced Welding and Joining Harbin Institute of Technology(Shenzhen)Shenzhen 518055China Flexible Printed Electronic Technology Center School of Materials Science and EngineeringHarbin Institute of Technology(Shenzhen)Shenzhen 518055China

Surface-enhanced Raman spectroscopy(SERS),as a highly sensitive molecular analysis technique,can realize fast and non-destructive detection of the information of molecular bonds to identify the component of analytes by"fingerprint"*** preparation of SERS substrates plays an extremely important role in the development of SERS technology and the application of SERS *** integrating SERS enhancement substrates into microfluidic chips,researchers have developed the microfluidic SERS chips which expand the function of microfluidic chips and provide an efficient platform for on-site biochemical analysis equipped with the powerful sensing capability of SERS *** this paper,we will first briefly give a review of the current microfluidic SERS-active substrates preparation technology and present the perspective on the application prospects of microfluidic SERS-active *** then the challenges in the preparation of microfluidic SERS-active substrates will be pointed out,as well as realistic issues of using this technology for biochemical application.

关键词： SERS substrate Microfluidics Biochemical sensing On-site analysis

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Expression of serine protease SNC19/matriptase and its inhibitor hepatocyte growth factor activator inhibitor type 1 in normal and malignant tissues of gastrointestinal tract

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World Journal of Gastroenterology 2005年第39期11卷 6202-6207页

作者： Lei zeng Jiang Cao xing Zhang First Affiliated Hospital College of Medicine Zhejiang University Hangzhou 310003 Zhejiang Province China Clinical Research Institute Sir Run Run Shaw Hospital Zhejiang University Hangzhou 310016 Zhejiang Province China

AIM： To provide the expression profile of serine protease SNC19/matriptase and its inhibitor hepatocyte growth factor activator inhibitor type 1 （HAI-1） in normal and malignant tissues of gastrointestinal tract at mRNA level for further study on their correlations with tumor progression and metastasis. METHODS： Total RNAs were prepared from 37 samples of colorectal cancer tissues, 40 samples of gastric cancer tissues, and their adjacent normal tissues. The expression of SNC19/matriptase and HAI-1 in these samples was detected by real-time fluorescent quantitative PCR using glyceraldehyde-3-phosphate dehydrogenase as internal standard, and the clinical significance for the correlation with clinicopathological parameters was evaluated. RESULTS： In gastric cancer tissues the expression of HAI-1 and SNC19/matriptase was significantly lower than that in the corresponding adjacent normal tissues （Z = -3.280, P= 0.006; Z= -4.651, P= 0.000）. HAI-1：SNC19/matriptase ratio showed no difference between normal and malignant tissues （P〉0.05）. Analysis of clinicopathological parameters showed decreased expression of HAI-1 and HAI-1：SNC19/ matriptase ratio associated with stage Ⅲ/Ⅳ gastric tumors as compared to stage Ⅰ/Ⅱ ones （Z= -2.140, P= 0.031; Z = -2.155, P = 0.031）, and with lymph node-positive gastric cancer tissues as compared to lymph node-negative ones （Z = -2.081, P = 0.036; Z= -2.686, P = 0.006）. The expression of SNC19/matriptase had no relationship with stages and lymph node metastasis （P〉0.05）. The expression of HAI-1 and HAI-1：SNC19/matriptase ratio increased in well-differentiated gastric cancer tissues, but there was no statistical significance （P〉0.05）. The difference of SNC19/matriptase expression was not significant in gastric cancer tissues of different histological differentiation status （P〉0.05）. In colorectal cancer tissues, the expression of HAI-1 and SNC19/matriptase was also markedly lower than that in their adjacent normal tissues （Z= -3.100, P = 0.002;

关键词： Matriptase Hepatocyte growth factor activator inhibitor type 1 Expression Metastasis

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The potential mechanism and clinical application value of remote ischemic conditioning in stroke

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Neural Regeneration Research 2025年第6期20卷 1613-1627页

作者： Yajun Zhu Xiaoguo Li xingwei Lei Liuyang Tang Daochen Wen Bo zeng Xiaofeng Zhang Zichao Huang Zongduo Guo The First Affiliated Hospital of Chongqing Medical University ChongqingChina

Some studies have confirmed the neuroprotective effect of remote ischemic conditioning against stroke. Although numerous animal researches have shown that the neuroprotective effect of remote ischemic conditioning may be related to neuroinflammation, cellular immunity, apoptosis, and autophagy, the exact underlying molecular mechanisms are unclear. This review summarizes the current status of different types of remote ischemic conditioning methods in animal and clinical studies and analyzes their commonalities and differences in neuroprotective mechanisms and signaling pathways. Remote ischemic conditioning has emerged as a potential therapeutic approach for improving stroke-induced brain injury owing to its simplicity, non-invasiveness, safety, and patient tolerability. Different forms of remote ischemic conditioning exhibit distinct intervention patterns, timing, and application range. Mechanistically, remote ischemic conditioning can exert neuroprotective effects by activating the Notch1/phosphatidylinositol 3-kinase/Akt signaling pathway, improving cerebral perfusion, suppressing neuroinflammation, inhibiting cell apoptosis, activating autophagy, and promoting neural regeneration. While remote ischemic conditioning has shown potential in improving stroke outcomes, its full clinical translation has not yet been achieved.

关键词： Akt apoptosis autophagy cerebral perfusion cerebral vascular stenosis clinical transformation hemorrhagic stroke ischemic stroke neuroinflammation neuroprotection Notch1 PI3K remote ischemic conditioning stroke

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