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Ginsenoside Rg_3 inhibit hepatocellular carcinoma growth via intrinsic apoptotic pathway

World Journal of Gastroenterology 2011年第31期17卷 3605-3613页

作者： Jian-Wen Jiang xin-Mei chen xin-hua chen Shu-Sen Zheng The Key Lab of Combined Multi-organ Transplantation Ministry of Public HealthThe Department of Hepatobiliary and Pancreatic SurgeryFirst Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310003Zhejiang ProvinceChina The Department of Pharmacy Shandong Traditional Chinese Medicine UniversityJinan 250355Shandong ProvinceChina

AIM:To investigate the anti-tumor function of ginsenoside Rg3 on hepatocellular carcinoma(HCC) in vitro and in vivo,and its ***:Hep1-6 and HepG2 cells were treated by Rg3 in different concentrations(0,50,100 and 200 μg/mL) in *** incubation for 0,6,12,24 and 48 h,cell viability was measured by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide *** was identified by terminal deoxynucleotidyl transferasemediated dUTP-biotin nick end ***-3 activity was measured by chromophore p-nitroanilide and flow ***-2 family proteins were ascertained by *** membrane potentialwas detected by 5,5',6' 6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine *** liver tumor-bearing C57Bl6 mice were divided randomly into 4 groups for intra-tumor injection of saline,ginsenoside Rg3,cyclophosphamide(CTX) and ginsenoside Rg3 + CTX ***:The survival time was followed up to 102 *** mice in the Rg3 + CTX group showed significant increased survival time compared with those in the control group(P < 0.05).Rg3 could inhibit HCC cell proliferation and induce cell apoptosis in vitro in the concentration and time dependent *** also induced mitochondria membrane potential to ***-3 activation can be blocked by the inhibitor *** was up-regulated while Bcl-2 and Bcl-XL were down-regulated after Rg3 ***:Our data suggested that Rg3 alone or combined with CTX inhibited tumor growth in vivo and prolonged mouse survival time by inducing HCC cell apoptosis via intrinsic pathway by expression alterations of Bcl-2 family proteins.

关键词： Ginsenoside Rg3 Apoptosis Hepatocellular Carcinoma Bcl-2 family proteins Cyclophosphamide

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Improved experimental model of hepatic cystic hydatid disease resembling natural infection route with stable growing dynamics and immune reaction

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World Journal of Gastroenterology 2017年第45期23卷 7989-7999页

作者： Rui-Qing Zhang xin-hua chen Hao Wen Hepatobiliary and Hydatid Depart- ment Digestive and Vascular Surgery CentreXinjiang Key Laboratory of EchinococcosisThe First Affiliated Hospital of Xinjiang Medical University Department of Hepatobiliary and Pancreatic Surgery The First Affiliated HospitalZhejiang University Key Laboratory of Combined Multi-organ Transplantation Ministry of Public HealthThe First Affiliated HospitalZhejiang University Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases The First Affiliated HospitalZhejiang University

AIM To investigate a safer way to set up the disease model of cystic echinococcosis without contamination risk and develop a novel experimental murine model of hepatic cystic echinococcosis. METHODS C57 B/6 mice were injected with human protoscolices of three different concentrations via the portal vein. The mice were followed for 10 mo by ultrasound,gross anatomy,and pathological and immunological examinations. The protoscolex migration in the portal vein,hydatid cyst growth,host immune reaction,and hepatic histopathology were examined *** The infection rates in the mice in the high,medium,and low concentration groups were 90%,100%,and 63.6%,respectively. The protoscolices migrated in the portal vein with blood flow,settled in the liver,and developed into orthotopic hepatic hydatid cysts,resembling the natural infection route and *** We have established an improved experimental model of hepatic cystic echinococcosis with low biohazard risk but stable growing dynamics and immune reaction. It is especially useful for new anti-parasite medication trials against hydatid disease.

关键词： Echinococcosis Echinococcosis granulosus Protoscolex Hydatid disease Experimental model

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Gut microbial dysbiosis associates hepatocellular carcinoma via the gut-liver axis

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Hepatobiliary & Pancreatic Diseases International 2019年第1期18卷 19-27页

作者： Jian-Wen Jiang xin-hua chen Zhigang Ren Shu-Sen Zheng Department of Hepatobiliary and Pancreatic Surgery Key Laboratory of Combined Multi-organ Transplantation Ministry of Public Health First Affiliated Hospital Zhejiang University School of Medicine Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Zhejiang University Health Management Center First Affiliated Hospital Zhejiang University School of Medicine Department of Infectious Disease Precision Medicine Center the First Affiliated Hospital of Zhengzhou University

Background: Hepatocellular carcinoma(HCC) is one of the most common malignancies in the world. Gut microbiota has been demonstrated to play a critical role in liver inflammation, chronic fibrosis, liver cirrhosis, and HCC development through the gut-liver axis. Data sources: Recently there have been several innovative studies investigating gut microbial dysbiosismediated enhancement of HCC through the gut-liver axis. Literatures from January 1998 to January 2018 were searched in the Pub Med database using the keywords "gut microbiota" and "hepatocellular carcinoma" or "liver cancer", and the results of experimental and clinical studies were analyzed. Results: Gut microbial dysbiosis accompanies the progression of alcoholic liver disease, non-alcoholic fatty liver disease and liver cirrhosis, and promotes HCC progression in an experimental mouse model. The immune system and key factors such as Toll-like receptor 4 are involved in the process. There is evidence for gut microbial dysbiosis in hepatitis virus-related HCC patients. Conclusions: Gut microbial dysbiosis is closely associated with hepatic inflammation disease and HCC through the gut-liver axis. With the enhanced understanding of the interactions between gut microbiota and liver through the gut-liver axis, new treatment strategies for HCC are being developed.

关键词： Gut-liver axis Gut microbiota Hepatocellular carcinoma Lipopolysaccharide Toll-like receptor 4

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Is irreversible electroporation safe and effective in the treatment of hepatobiliary and pancreatic cancers?

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Hepatobiliary & Pancreatic Diseases International 2019年第2期18卷 117-124页

作者： Li-Ming Wu Le-Le Zhang xin-hua chen Shu-Sen Zheng Division of Hepatobiliary and Pancreatic Surgery the First Affiliated Hospital Zhejiang University School of Medicine

Background: Irreversible electroporation(IRE) is a novel ablative technique for hepatobiliary and pancreatic cancers. This review summarizes the data regarding the safety and efficacy of IRE in the treatment of hepatobiliary and pancreatic cancers. Data sources: Studies were identified by searching Pub Med and Embase for articles published in English from database inception through July 31, 2017. For inclusion, each clinical study had to report morbidity and survival data on hepatobiliary and pancreatic cancers treated with IRE and contain at least 10 patients. Studies that met these criteria were included for analysis. Two authors assessed each clinical study for data extraction. The controversial parts were resolved through discussion with seniors. Results: A total of 24 clinical studies were included. Fourteen focused on hepatic ablation with IRE comprising 437 patients with 666 lesions of different tumor types. Two patients(0.5%) died after the IRE procedure. Morbidity of hepatic ablation with IRE ranged from 7% to 35%. Most complications were mild. Complete response for hepatic tumors was reported as 57%–97%. Ten studies with 455 patients focused on pancreatic IRE. The overall mortality of IRE in pancreatic cancer was 2%. Overall severe morbidity of IRE in pancreatic cancer ranged from 0 to 20%. The median overall survival after IRE ranged from 7 to 23 months. Patients treated with IRE combined with surgical resection showed a longer overall survival. Conclusions: IRE significantly improves the prognosis of advanced hepatobiliary and pancreatic malignances, and companied with less complications. Hence, IRE is a relatively safe and effective non-thermal ablation strategy and potentially recommended as an option for therapy of patients with hepatobiliary and pancreatic malignances.

关键词： Irreversible electroporation Hepatic cancer Pancreatic cancer

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Screening of Highly Effective Sinorhizobium meliloti Strains for 'Vector' Alfalfa and Testing of Its Competitive Nodulation Ability in the Field

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Pedosphere 2007年第2期17卷 219-228页

作者： ZENG Zhao-Hai chen Wen-xin HU Yue-Gao SUI xin-hua chen Dan-Ming College of Agronomy and Biotechnology China Agricultural University Beijing 100094 （China） College of Biology/Key Laboratory of Agro-Microbial Resource and Application Ministry of Agriculture China Agricultural University Beijing 100094 （China）

Seventeen Sinorhizobium meliloti strains from seven provinces in China were used to screen highly effective strains for alfalfa cultivar in a greenhouse study and their symbiotic relationship and competitive ability were studied in the field. CCBAU30138 was the most effective strain, as evidenced by increase in dry weights. A field experiment showed that the inoculation of alfalfa with CCBAU30138 resulted in increases of 11.9% and 19.6% of dry matter production and crude protein production, respectively, in forage of monocultured plants. The total dry matter yields of alfalfa and tall fescue in binary culture were increased by 16.3% by inoculation of alfalfa with this strain. These results showed that S. rneliloti strain CCBAU30138 was an effective inoculant both in the greenhouse and in the field. The analysis of randomly amplified polymorphic DNA （RAPD） by polymerase chain reaction （PCR） from nodule extracts showed that the strain CCBAU30138 had high competitiveness in the field. It occupied 47.5% of nodules in alfalfa monoculture and 44.4% of nodules in alfalfa-tall fescue binary culture after 20 weeks of growth. In conclusion, a simple system to select highly effective and competitive symbiotic strains specific to alfalfa was established. Using this system, a *** suitable for the alfalfa cultivar ‘Vector＇ grown in Wuqiao County of Hebei Province was obtained.

关键词： alfalfa inoculant selection Sinorhizobium meliloti tall fescue

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The safety of esophagojejunostomy via a transorally inserted-anvil method vs extracorporeal anastomosis using a circular stapler during total gastrectomy for Siewert type 2 adenocarcinoma of the esophagogastric junction

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Gastroenterology Report 2020年第3期8卷 242-251,I0002页

作者： xin-hua chen Yan-Feng Hu Jun Luo Yue-Hong chen Hao Liu Tian Lin Hao chen Guo-xin Li Jiang Yu Department of General Surgery Nanfang HospitalSouthern Medical UniversityGuangzhouGuangdongP.R.China

Background:Intracorporeal esophagojejunostomy via a transorally inserted-anvil method during laparoscopic total gastrectomy(LTG)for upper gastric cancer has been demonstrated to be feasible,but the use of this assessment exclusively for Siewert type 2 adenocarcinoma of the esophagogastric junction(AEG)has not been ***:A total of 428 consecutive gastric-cancer patients who underwent LTG in Nanfang Hospital from January 2008 to December 2016 were *** these patients,98 were classified as Siewert type 2 *** patients underwent intracorporeal esophagojejunostomy through either a transorally inserted-anvil method(n=27)or extracorporeal anastomosis usinga circular stapler(n=71).After generating propensity scores with covariates that were associated with developing anastomotic leakage,26 patients who underwent esophagojejunostomy via the transorally inserted-anvil method(transoral group)were 1:1 matched with 26 patients who underwent the procedure via extracorporeal anastomosis using a circular stapler(extracorporeal group).The safety after 30 days post-operatively was compared between the two ***:The transoral group and extracorporeal group were balanced regarding the baseline *** operative time,reconstruction duration,number of dissected lymph nodes,length of the proximal resectionmargins,estimated blood loss,intra-operative complication rate,and post-operative recovery course were not significantly different between the two *** mean anvil-insertion completion time(9.763.0 vs 13.462.0minutes,P<0.001)and the median incision length(5.5 vs 7.0 cm,P<0.001)in the transoral group were shorter than those in the extracorporeal *** incidence of post-operative complications(26.9%vs 23.1%,P=0.749)and the classification of complication severity(P=0.939)were similar between the two ***:Intracorporeal esophagojejunostomy through a transorally inserted-anvil method may be a potentially safe approach to simplify

关键词： adenocarcinoma of the esophagogastric junction laparoscopic total gastrectomy esophagojejunostomy anastomotic leakage

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Associations between obesity and metabolic health with nonalcoholic fatty liver disease in elderly Chinese

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Hepatobiliary & Pancreatic Diseases International 2020年第3期19卷 252-257页

作者： Li-Ming Wu Hui He Gang chen Yu Kuang Bing-Yi Lin xin-hua chen Shu-Sen Zheng Department of Hepatobiliary and Pancreatic Surgery The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China Department of Internal Medicine Shaoxing Keqiao Women and Children’s HospitalShaoxing 312030China Medical Physics Program University of NevadaLas VegasNV 89121USA

Background:Nonalcoholic fatty liver disease(NAFLD)is closely associated with ***,this association could be influenced by the coexisting metabolic *** study aimed to investigate the role of obesity and metabolic abnormalities in NAFLD among elderly ***:A cross-sectional study was performed among elderly residents who took their annual health checkups during 2016 in Keqiao District,Shaoxing,***:A total of 3359 elderly adults were retrospectively included in this *** overall prevalence of NAFLD was 28.7%.The prevalence of NAFLD were 7.14%,27.92%,34.80%,and 61.02%in participants with metabolically healthy normal weight(MHNW),metabolically abnormal normal weight(MANW),metabolically healthy obese(MHO),and metabolically abnormal obese(MAO),*** patients in MHO group had more unfavorable metabolic profiles than those in MHNW *** regression analysis showed that sex,body mass index(BMI),fasting blood glucose,and serum uric acid were the risk factors of ***:Both obesity and metabolic health were significantly associated with NAFLD in elderly *** for obesity and other metabolic abnormalities should be routinely performed for early risk stratification of NAFLD.

关键词： Fatty liver disease Obesity Metabolic health Elderly

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Prevalence of and risk factors for non-alcoholic fatty liver disease in a Chinese population: An 8-year follow-up study

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World Journal of Gastroenterology 2016年第13期22卷 3663-3669页

作者： Zhen-Ya Lu Zhou Shao Ya-Li Li Muhuyati Wulasihan xin-hua chen Department of Internal Medicine the First Affiliated Hospital Zhejiang University Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Zhejiang University Department of Hepatobiliary and Pancreatic Surgery the First Affiliated Hospital Zhejiang University Department of Internal Medicine the First Affiliated Hospital Xinjiang Medical University

AIM: To investigate the prevalence of and risk factors for non-alcoholic fatty liver disease(NAFLD) in a Chinese ***: A total of 1948 adults from China was followed for 8 years. A cross-sectional study was performed to investigate the prevalence of NAFLD at baseline, and then the participants were followed for 8 years to investigate risk factors for the development of ***: A total of 1948 participants were enrolled at baseline, of whom 691 were diagnosed with NAFLD. During the 8-year follow-up, 337 baseline NAFLD-free participants developed NAFLD. They had a greaterincrease in body mass index(BMI), serum uric acid, fasting plasma glucose, very low-density lipoprotein cholesterol and a considerable decrease in high-density lipoprotein cholesterol. 123 participants who had NAFLD at baseline lost NAFLD during the 8-year follow-up period. They had a greater decrease in BMI, fasting plasma glucose, triglycerides, total cholesterol, low-density lipoprotein cholesterol, alanine aminotransferase, aspartate aminotransferase, and γ-glutamyl ***: NAFLD is prevalent in Chinese population with a rapidly increasing tendency. It can be reversed when patients lose their weight, control their hyperlipidemia and hyperglycemia, and reduce the liver enzyme levels.

关键词： Non-alcoholic fatty liver disease Follow-up Prevalence Risk factors

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Recent progress in pulsed electric field ablation for liver cancer

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World Journal of Gastroenterology 2020年第24期26卷 3421-3431页

作者： Zhen-Guo Liu xin-hua chen Zu-Jiang Yu Jun Lv Zhi-Gang Ren Department of Infectious Diseases the First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052Henan ProvinceChina Gene Hospital of Henan Province Zhengzhou 450052Henan ProvinceChina Precision Medicine Center the First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052Henan ProvinceChina Department of Hepatobiliary and Pancreatic Surgery the First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310003Zhejiang ProvinceChina Key Laboratory of Combined Multi-organ Transplantation Ministry of Public HealthHangzhou 310003Zhejiang ProvinceChina Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Hangzhou 310003China

The number of liver cancer patients is likely to continue to increase in the coming decades due to the aging of the population and changing risk *** treatments cannot meet the needs of all *** treatment methods evolved from pulsed electric field ablation are expected to lead to breakthroughs in the treatment of liver *** paper reviews the safety and efficacy of irreversible electroporation in clinical studies,the methods to detect and evaluate its ablation effect,the improvements in equipment and its antitumor effect,and animal and clinical trials on *** also summarize studies on the most novel nanosecond pulsed electric field ablation techniques in vitro and in *** research results are certain to promote the progress of pulsed electric field in the treatment of liver cancer.

关键词： Hepatocellular carcinoma Pulsed electric field Irreversible electroporation Electrochemotherapy Nanosecond pulsed electric fields Ablation treatment

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Gastrointestinal tract post-transplant lymphoproliferative disorder after liver transplantation

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Hepatobiliary & Pancreatic Diseases International 2017年第5期16卷 556-558页

作者： Qin-Fen Xie Ping chen xin-hua chen Ji-Min Liu Jan Lerut Shu-Sen Zheng Department of Hepatobiliary and Pancreatic Surgery Shulan(Hangzhou) Hospital (Zhejiang University International Hospital) Hangzhou 310000 China Pancreatic Surgery and Department of Infectious Disease Shulan(Hangzhou) Hospital (Zhejiang University International Hospital) Hangzhou 310000 China Key Laboratory of Combined Multi-organ Transplantation Ministry of Public Health Department of Hepatobiliary and Pancreatic Surgery First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China Department of Pathology and Molecular Medicine Faculty of Health Science McMaster UniversityHamilton Ontario Canada Starzl Unit of Abdominal Transplantation Université catholique Louvain (UCL) Brussels Belgium

To the Editor:Post-transplant lymphoproliferative disorder（PTLD）is a rare and potentially fatal complication occurring after all types of solid organ transplantation.;PTLD accounts for 20%of all de novo post-transplant tumors.;The most important risk factors for PTLD are prolonged intense immunosuppression and Epstein-Barr virus（EBV）

关键词： DLBCL EBV Gastrointestinal tract post-transplant lympho proliferative disorder after liver transplantation HBV MOP BPR GI OLT

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