检索结果-南通市图书馆

Molecular mechanism of temperature-dependent sex determination and differentiation in Chinese alligator revealed by developmental transcriptome profiling

在线全文

学校读者我要写书评

暂无评论

Science Bulletin 2018年第4期63卷 209-212页

作者： Jian-Qing Lin Qi Zhou hai-Qiong Yang Li-Ming Fang Ke-Yi Tang Li sun Qiu-Hong Wan Sheng-Guo Fang The Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and State Conservation Center for Gene Resources of Endangered WildlifeCollege of Life SciencesZhejiang UniversityHa ngzhou 310058China Life Sciences Institute Zhejiang UniversityHangzhou 310058China Changxing Yinjiahian Chinese Alligator Nature Reserve Changxing 313100China

Sex determination(SD)is a critical embryonic development process that has profound impacts on the morphology of individuals and the survival of populations[1].In vertebrates,sex can be determined by either genetic or environmental factors[2],with the underlying molecular mechanism unknown for most species except for a few model organisms[3,4].Unlike mammals and birds,

关键词：温度依赖短吻鳄性别中国机制分子性染色体脊椎动物

Source of ore-forming material for the Huangtuliang gold deposit,Hebei Province and ore prospecting in the deep periphery

在线全文

学校读者我要写书评

暂无评论

Chinese Journal Of Geochemistry 2008年第3期27卷 225-234页

作者： NIU Shuyin sun Aiqun WANG Baode HAN Yuchou WEI Minghui ZHANG hai ZHANG Ge SHI Ping WANG Wenxing College of Resources Shijiazhuang University of Economics Shijiazhuang 050031 China Hebei Provincial Bureau of Geology and Mineral Resource Exploration and Development Shijiazhuang 050081 China Huangtuliang Gold Mine Chicheng County Zhangjiakou 075700 China

The Huangtuliang gold deposit is characterized by its wide and large ore belt,stable extension and closely spaced ***,no orebody was found by deep *** a result,ore prospecting in this region was once put into *** analysis of ore-forming and ore-controlling structures in the mining district by the authors has revealed that the ore-forming and ore-controlling structure in this mining district is a steeply dipping(85°-110°/∠70°-85° N-NNE),spade-shaped ductile shear zone,and the ore-controlling structures are a series of nearly erected second-ordered faults which are developed in the upper part of the ductile shear zone,intersecting with the ductile shear *** cutting of the ductile shear zone made it possible the ascending of ore fluids from the mantle plume at depth and these ore fluids would migrate upwards along the ductile shear zone under certain temperature and pressure *** their ascending path,the ore fluids would extract ore-forming elements from the country rocks and the extracted ore-forming elements would be deposited as ores in the hanging-wall second-ordered *** reason why no orebody was found in early prospecting at depth is that northward-dipping drilling in the southern part of the shear zone extended so deeply as to be beneath the shear *** shallow-level orebodies could be found by southward-dipping drilling practice in the northern part of the shear *** location where deep-seated orebodies occurred shifted northwards and the orebodies occurred at greater ***,it is natural that no orebody could be found when drill core passed through the shear *** the ore-forming and ore-controlling structures were well understood,the focus of ore prospecting was placed on the deep-level,northward-penetrating *** this way a number of new blind orebodies of great thickness have been *** the basis of research development in the mining district,a prospecting plan has

关键词：地幔分支结构地幔热流柱矿石河北

Stacked lateral double-diffused metal–oxide–semiconductor field effect transistor with enhanced depletion effect by surface substrate

在线全文

学校读者我要写书评

暂无评论

Chinese Physics B 2019年第3期28卷 328-332页

作者： Qi Li Zhao-Yang Zhang hai-Ou Li Tang-You sun Yong-He Chen Yuan Zuo Guangxi Key Laboratory of Precision Navigation Technology and Application Guilin University of Electronic Technology

A stacked lateral double-diffused metal–oxide–semiconductor field-effect transistor(LDMOS) with enhanced depletion effect by surface substrate is proposed(ST-LDMOS), which is compatible with the traditional CMOS processes. The new stacked structure is characterized by double substrates and surface dielectric trenches(SDT). The drift region is separated by the P-buried layer to form two vertically parallel devices. The doping concentration of the drift region is increased benefiting from the enhanced auxiliary depletion effect of the double substrates, leading to a lower specific on-resistance(Ron,sp). Multiple electric field peaks appear at the corners of the SDT, which improves the lateral electric field distribution and the breakdown voltage(BV). Compared to a conventional LDMOS(C-LDMOS), the BV in the ST-LDMOS increases from 259 V to 459 V, an improvement of 77.22%. The Ron,sp decreases from 39.62 m?·cm^2 to 23.24 m?·cm^2 and the Baliga's figure of merit(FOM) of is 9.07 MW/cm^2.

关键词： double substrates surface dielectric trench stacked lateral double-diffused metal–oxide– semiconductor field-effect transistor(ST-LDMOS) breakdown voltage

Surgical treatment ofpatients with severe non-flail chest rib fractures

在线全文

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2019年第22期7卷 3718-3727页

作者： Jian-Peng Zhang Lin sun Wei-Qiang Li Yan-Yu Wang xin-Zhen Li Yang Liu Department of Thoracic Surgery Beijing Luhe Hospital Capital Medical University

BACKGROUND Many patients have inadequate long-term analgesia,respiratory distress,and hypoxemia due to a long-standing substantial smoking history or the presence of primary pulmonary diseases;analgesic treatment is not valid in these *** if the imaging findings of rib fractures are relatively mild,rib fractures may cause severe position limitation,respiratory distress,and *** To investigate the curative effect of surgical treatment for patients with severe non-flail chest rib *** A total of 78 patients from our hospital with severe noncontinuous thoracic rib fractures from September 2016 to September 2018 were enrolled in our ***-nine patients underwent surgical treatment,and 39 underwent conservative *** surgical treatment group received surgery performed with titanium plates,and the screws were inserted with open reduction and internal *** conservative treatment group received analgesia and symptomatic *** pain scores at 72 h,1 wk,2 wk,4 wk,6 wk,3 mo,and 6 mo were compared,and the SF-36 quality of life scores were compared atthe 3rd and 6th *** Pain relief in the surgical group was significantly better than that in the conservative group at each time point(72 h,1 wk,2 wk,4 wk,6 wk,3 mo,and 6 mo after surgery,P<0.001).The SF-36 scores were significantly higher in the surgical group than in the conservative group at 1 mo and 6 mo(P<0.05).CONCLUSION Patients with severe non-flail chest rib fractures have a better quality of life following surgical treatment than following conservative treatment,and surgical treatment is also useful for relieving *** should pay more attention to the physiological functions and clinical manifestations of patients with severe rib *** patients with non-flail chest rib fractures,surgical treatment is feasible and effective.

关键词： Severe Non-flail chest rib fractures Treatment Conservative surgery Internal fixation Quality of life

Celastrol inhibits migration, proliferation and transforming growth factor-β2-induced epithelial-mesenchymal transition in lens epithelial cells

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2019年第10期12卷 1517-1523页

作者： Li-Ping Wang Bao-xin Chen Yan sun Jie-Ping Chen Shan Huang Yi-Zhi Liu State Key Laboratory of Ophthalmology Zhongshan Ophthalmic Center Sun Yat-sen University

AIM: To investigate the mechanism of celastrol in inhibiting lens epithelial cells(LECs) fibrosis, which is the pathological basis of ***: Human LEC line SRA01/04 was treated with celastrol and transforming growth factor-β2(TGF-β2). Wound-healing assay, proliferation assay, flow cytometry, real-time polymerase chain reaction(PCR), Western blot and immunocytochemical staining were used to detect the pathological changes of celastrol on LECs. Then, we cultured Sprague-Dawley rat lens in medium as a semi-in vivo model to find the function of celastrol ***: We found that celastrol inhibited the migration of LECs, as well as proliferation(P<0.05). In addition, it induced the G2/M phase arrest by cell cyclerelated proteins(P<0.01). Moreover, celastrol inhibited epithelial-mesenchymal transition(EMT) by the blockade of TGF-β/Smad and Jagged/Notch signaling ***: Our study demonstrates that celastrol could inhibit TGF-β2-induced lens fibrosis and raises the possibility that celastrol could be a potential novel drug in prevention and treatment of fibrotic cataract.

关键词： lens cataract fibrosis transforming growth factor-β2 celastrol

Analysis of nucleosome positioning in promoters of miRNA genes and protein-coding genes

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2010年第22期55卷 2347-2352页

作者： LIU HongDe ZHANG DeJin XIE JianMing YUAN ZhiDong MA xin LU ZhiYuan GONG LeJun sun Xiao State Key Laboratory of Bioelectronics Southeast University Nanjing 210096 China

Nucleosome positioning in promoters is important for gene transcription regulation. In this paper, with a nucleosome prediction model, curvature profile, the characteristics of nucleosome positioning in promoters are analyzed for miRNA genes and protein-coding genes. In the vicinity of transcription start site (TSS), there is a nucleosome-free region (NFR) followed by a positioned nucleosome at ～200 bp downstream of TSS. A similar characteristic is observed in independent intronic promoters and intergenic promoters, namely, both types of promoters have a longer NFR in 0—-400 bp upstream of TSS. Moreover, transcription factor binding sites (TFBSs) locate in the NFR with a high concentration. However, nucleosome pattern in dependent intronic promoters are like that in protein-coding promoters, with two nucleosomes positioned at -200—-400 bp and -400—-600 bp upstream of TSS. The results indicate nucleosome positioning is probably different in independent miRNA promoters and protein-coding promoters; and positioning seems to be an important factor not only in regulation of protein-coding gene, but also in that of miRNA gene.

关键词：蛋白编码基因基因启动子核小体点定位 RNA基因 miRNA 蛋白质编码转录起始位点

Sanchen powder extract combined with vancomycin against methicillin-resistant Staphylococcus aureus

在线全文

学校读者我要写书评

暂无评论

Journal of Traditional Chinese Medical Sciences 2022年第2期9卷 181-187页

作者： Jiajia Fan Honghao sun Yuanyuan Liu xin Li Huichao Wu Xiaoqiao Ren School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing102488China

Objective: To explore the effects of the extract of Sanchen powder(ESCP) combined with vancomycin on methicillin-resistant Staphylococcus aureus(MRSA) planktonic cells, biofilms, and virulence ***: The herbs in Sanchen powder(SCP) were extracted separately with 50% ethanol. Then, the content of hydroxysafflower yellow A(HSYA) and cholic acid in the extract of artificial cattle bezoar(artificial Calculus bovis) and safflower(Carthamus tinctorius L.) was measured by ultraviolet visible-vis spectrophotometry and high-performance liquid chromatography. The effects of ESCP combined with vancomycin on MRSA by observing its biofilm viability were assessed using a 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium5-carboxanilide reduction assay and scanning electron ***, enzyme activity was measured by plasma coagulase test and DNase ***: The cholic acid content of the artificial C. bovis extract was 7.34(0.81) mg/g, and the HSYA content of the C. tinctorius extract was 9.18(0.09) mg/g. The minimal inhibitory concentrations of ESCP and vancomycin were 25.6 mg/m L and 2 μg/m L. The minimum bactericidal concentration of ESCP was51.2 mg/m L. ESCP combined with vancomycin could inhibit the expression of coagulase and bacteria in mature biofilms. Neither ESCP nor vancomycin had a significant effect on ***: This study is the first to show that ESCP combined with vancomycin inhibits coagulase and MRSA embedded in mature biofilms and that it represents a promising treatment for MRSA infection.

关键词： Tibetan medicine Sanchen powder Vancomycin Methicillin-resistant Staphylococcus aureus Antibacterial effect Traditional Chinese medicine

CLINICAL APPLICATION OF BODY DIFFUSION WEIGHTED MR IMAGING IN THE DIAGNOSIS AND PREOPERATIVE N STAGING OF CERVICAL CANCER

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Sciences Journal 2008年第3期23卷 133-137页

作者： Hua-dan Xue Shuo Li Fei sun Hong-yi sun Zheng-yu Jin Jia-xin Yang Mei Yu Department of Radiology Peking Union Medical College Hospital Chinese Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100730 General Electric Medical Solution Shanghai 200336 Department of Gynecology and Obstetrics Peking Union Medical College Hospital Chinese Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100730

Objective To evaluate the clinical impact of body diffusion weighted imaging (DWI) on the diagnosis and preoperative N staging of cervical cancer. Methods Twenty-four patients (mean age 37.9 years old) with proved cervical cancer by cervical biopsy and 24 female patients with other suspected pelvic abnormalities received preoperative body DWI scan. Results of body DWI were compared with pathological findings. The apparent diffusion coefficient (ADC) values of normal cervix and different pathological types of cervical cancer were compared. ADC value of normal or inflammatory lymph nodes was also compared with that of metastatic ones. Student's t test was used for statistical analysis. Results There were 5 adenocarcinomas and 19 epitheliomas showed with biopsy results, and DWI showed 21 cervical lesions out of them (87.5%). ADC values of the normal cervix (n = 24), epithelioma (n = 19), and adenocar- cinoma (n = 5) were (1.73 ± 0.31) ×10-3, (0.88 ± 0.22) ×10-3, and (1.08 ± 0.12) ×10-3 mm2/s, respectively. Statistical analysis showed significant difference in ADC value between normal cervical tissue and either tumor tissues (both P < 0.01). In patients had lymphadenectomy (n = 24), totally 67 lymph nodes including 16 metastatic lymph nodes were pathologically analyzed, and DWI showed 66 (98.5%) out of them. ADC values of normal/inflammatory and metastatic lymph nodes were (1.07 ± 0.16) ×10-3 and (0.77 ± 0.13) ×10-3 mm2/s (P < 0.01). Receiver operating characteristic (ROC) curve of ADC value of metastatic lymph node showed that area under curve was 0.961. Conclusions ADC value in cervical carcinoma is lower than that in normal cervix, and ADC may have predictive value in subtype discrimination. ADC value may improve the preoperative characterization of lymph node metastasis. And at least abdominal and pelvic DWI scan is suggested for N staging evaluation in such patients.

关键词： magnetic resonance diffusion weighted imaging cervical cancer

Evaluation of Pre-Harvest Sprouting(PHS)Resistance and Screening of High-Quality Varieties from Thirty-Seven Quinoa(Chenopodium quinoa Willd.)Resources in Chengdu Plain

在线全文

学校读者我要写书评

暂无评论

国际实验植物学杂志(英文) 2023年第10期92卷 2921-2936页

作者： xin Pan Ya Gao Fang Zeng Chunmei Zheng Wenxuan Ge Yan Wan Yanxia sun Xiaoyong Wu Key Laboratory of Coarse Cereal Processing Ministry of Agriculture and Rural AffairsChengdu UniversityChengdu610106China

Pre-harvest sprouting(PHS)will have a serious effect both on the yield and quality of quinoa(Chenopodium quinoaWilld.).It is crucial to select and breed quinoa varieties with PHS resistance and excellent agronomic traits for guidance production and utilization of quinoa.A comprehensive evaluation of the PHS resistance and agronomic traits of 37 species of quinoa resources was conducted in Chengdu *** evaluation used various methods,including grain germination rate(GR),grain germination index(GI),total spike germination rate(SR),total grain germination index(SI),grey correlation analysis(GCA),cluster analysis and correlation *** showed significant differences in PHS resistance and agronomic traits amongst the 37 quinoa ***-23 was most resistant to PHS within 24 h,with a germination rate of 2.67%and 0%according to the GR and SR results,***,in the same time,CDU-31 showed the maximum susceptibility to PHS based on the SR of 31.07%,while CDU-34 was the most sensitive to PHS according to the GR of 100%.The comprehensive evaluation identified one and nine kinds of high resistance species for grain and whole spike germination,*** both cases,the coefficients of variation(CV)for these parameters were 34.78%and 82.13%,*** results showed that the magnitude of the association between each trait and yield in the thirty-seven quinoa resources was in the following order:thousand grain weight>seed length>seed area>seed *** the seed weight of CDU-18 reached 3.7010 g,the seed weight of CDU-5 was only 1.6030 ***,the size of the seeds,their width and area did not correlate with their 1000-grain *** was a complex correlation between PHS resistance index and agronomic *** on clustering analysis,thirty-seven quinoa resources were classified into three *** was found that various taxa differed in PHS resistance and agronomic *** comparisons of the aggregated data led to th

关键词： Quinoa pre-harvest sprouting(PHS) agronomic traits cluster analysis