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Stress Responsive Zinc-finger Protein Gene of Populus euphratica in Tobacco Enhances Salt Tolerance

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Journal of Integrative Plant Biology 2008年第1期50卷 56-61页

作者： Jun-Ying Wang xin-Li xia Jun-Ping Wang Wei-Lun Yin Biotechnology Research Institute of Chinese Academy of Agncultural Sciences Beijing 100081 China Beijing Forestry University Beijing 100083 China Tianjin University of Science and Technology Tianjin 300222 China

The Populus euphratica stress responsive zinc-finger protein gene PSTZ, which encodes a protein including typical Cys2/His2 zinc finger structure, was isolated by reverse transcription-polymerase chain reaction from P. euphratica. Northern hybridization revealed that its expression was induced under drought and salt stress conditions. To examine its function, cDNA of the PSTZ gene, driven by the cauliflower mosaic virus 35S promoter, was cloned into a plant expression vector pBin438 and introduced into tobacco plants. Transgenic tobacco showed an enhanced salt tolerance, suggesting that PSTZ may play a role in plant responsiveness to salt stress.

关键词： enhance Populus euphratica, salt tolerance stress responsive zinc-finger protein.

Endoscopic and surgical treatment of jejunal gallstone ileus caused by cholecystoduodenal fistula:A case report

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World Journal of Clinical Cases 2023年第17期11卷 4159-4167页

作者： Wen-Juan Fan Mei Liu xin-xia Feng Department of Gastroenterology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030Hubei ProvinceChina

BACKGROUND Gallstone ileus is a rare complication of gallstone disease in which a stone enters the enteric lumen and causes mechanical obstruction usually by bilioenteric *** ileus accounts for 25%of all bowel obstructions among the population>65 years of *** medical advances over the last decades,gallstone ileus is still associated with high rates of morbidity and *** SUMMARY An 89-year-old man with a history of gallstones was admitted to the Gastroenterology Department of our hospital,complaining of vomiting and cessation of bowel movements and *** computed tomography showed cholecystoduodenal fistula and upper jejunum obstruction due to gallstones,pneumatosis in the gallbladder,and pneumobilia indicating Rigler’s *** the high risk of surgical management,we performed propulsive enteroscopy and laser lithotripsy twice to relieve the bowel ***,the intestinal obstruction was not relieved by the less invasive ***,the patient was transferred to the Department of Biliary-pancreatic *** patient underwent the one-stage procedure including laparoscopic duodenoplasty(fistula closure),cholecystectomy,enterolithotomy,and *** surgery,the patient presented with complications of acute renal failure,postoperative leak,acute diffuse peritonitis,septicopyemia,septic shock,and multiple organ failure,and finally *** Early surgical intervention is the mainstay of treatment for gallstone *** elderly patients with significant comorbidities,enterolithotomy alone is advised.

关键词： Gallstone ileus Cholecystoduodenal fistula Pneumobilia Small bowel

Research on the cutting force of a pick

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Mining Science and Technology 2009年第4期19卷 514-517页

作者： LIU Song-yong DU Chang-long CUI xin-xia School of Mechanical and Electrical Engineering China University of Mining ＆ Technology Xuzhou Jiangsu 221008 China

Cutting tests were done using a test bed designed to measure pick cutting forces when cutting coal and *** test equipment has a drum with two starting helical *** forces on a pick were measured as a function of coal compressive strength,pick carbide tip diameter and the cutting depth per drum *** results show that the cutting force is linearly related to the compressive *** relationship between the cutting force and both the carbide tip diameter and the cutting depth are *** in the cutting force does not increase with coal compressive strength but it has a linear relationship to tip diameter.A plot of cutting force fluctuations versus the cutting depth follows a sigmoidal *** on the analysis of these test results a theoretical basis is supplied for design and effective use of shearer drums.

关键词： pick cutting force shearer cutting theory carbide tip diameter cutting depth

Levels of Crotonaldehyde and 4-hydroxy-(E)-2-nonenal and Expression of Genes Encoding Carbonyl-Scavenging Enzyme at Critical Node During Rice Seed Aging

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Rice science 2018年第3期25卷 152-160页

作者： FU Shenzao YIN Guangkun xin xia WU Shuhua WEI xinghua LU xinxiong State Key Laboratory of Rice Biology China National Rice Research Institute National Crop Genebank Institute of Crop Science Chinese Academy of Agricultural Sciences

The critical node(CN) is an important stage during seed aging, which is related to effective genebank conservation. Previous studies have demonstrated that proteins undergo carbonylated modification at the CN in rice, indicating oxidative damage. However, the levels of reactive carbonyl species(RCS) and the associated scavenging system at the CN are largely unknown. In this study, we optimized methods for the extraction and analysis of RCS from dry rice embryos. In order to acquire seeds at the CN, rice seeds were subjected to natural conditions for 7, 9, 11 and 13 months, and the seed germination rates were reduced to 90%, 82%, 71% and 57%, respectively. We chose the stage with seed germination rate of 82% as the CN according to the rice seed vigor loss curve. The levels of crotonaldehyde and 4-hydroxy-(E)-2-nonenal(HNE) were significantly increased at the CN. In addition, genes encoding carbonyl-scavenging enzyme, including Os ALDHs and Os AKRs, were significantly down-regulated at the CN, and reductions in the expression of Os ALDH2-2, Os ALDH2-5, Os ALDH3-4, Os ALDH7, Os AKR1 and Os AKR2 in particular could be responsible for RCS accumulation. Thus, the accumulations of crotonaldehyde and HNE and down-regulation of genes encoding carbonyl-scavenging enzyme might be related to an accelerating loss of seed viability at the CN.

关键词： carbonyl-scavenging system reactive carbonyl species seed aging crotonaldehyde critical node rice storage

Versatile metal graphitic nanocapsules for SERS bioanalysis

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Chinese Chemical Letters 2019年第9期30卷 1581-1592页

作者： Shengkai Li Jiamei Xu Shen Wang xin xia Long Chen Zhuo Chen Molecular Science and Biomedicine Laboratory (MBL) State Key Laboratory of Chemo/Bio-Sensing and Chemometrics College of Chemistry and Chemical Engineering Aptamer Engineering Center of Hunan Province Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology Hunan University Changsha 410082 China Faculty of Science and Technology University of Macao Taipa 999078 Macao China

The novel graphitic nanomaterial of metal graphitic nanocapsules(MGNs) with superior stability, unique optical properties and biocompatibility possess great potential in biomedical and bioanalytical applications. The graphitic shell can quench the background fluorescence interference from external environments via a fluorescence resonance energy transfer(FRET) process and even avoid unnecessary reactions catalyzed by inner metal core. The graphitic shell with several characteristic Raman bands itself can act as Raman signal probe or internal standard(IS), especially the 2D-band within the cellular Raman-silent region helps to reduce the interference signals from external conditions. The present context attempts to give a comprehensive overview about the preparation and unique properties of MGNs as well as their applications in SERS biodetection and bioimaging.

关键词： Versatile Metal graphitic nanocapsules SERS Biodetection Bioimaging

TagCombine： Recommending Tags to Contents in Software Information Sites

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Journal of Computer Science & Technology 2015年第5期30卷 1017-1035页

作者：王新宇夏鑫 David Lo 1.College of Computer Science and Technology Zhejiang University Hangzhou 310027 China 2.School of Information Systems Singapore Management University Singapore Singapore

Nowadays, software engineers use a variety of online media to search and become informed of new and interesting technologies, and to learn from and help one another. We refer to these kinds of online media which help software engineers improve their performance in software development, maintenance, and test processes as software information sites. In this paper, we propose TagCombine, an automatic tag recommendation method which analyzes objects in software information sites. TagCombine has three different components： 1） multi-label ranking component which considers tag recommendation as a multi-label learning problem; 2） similarity-based ranking component which recommends tags from similar objects; 3） tag-term based ranking component which considers the relationship between different terms and tags, and recommends tags after analyzing the terms in the objects. We evaluate TagCombine on four software information sites, Ask Different, Ask Ubuntu, Feecode, and Stack Overflow. On averaging across the four projects, TagCombine achieves recall@5 and recallS10 to 0.619 8 and 0.762 5 respectively, which improves TagRec proposed by Al-Kofahi et al. by 14.56% and 10.55% respectively, and the tag recommendation method proposed by Zangerle et al. by 12.08% and 8.16% respectively.

关键词： software information site online media tag recommendation

Quinoxaline-based Polymers with Asymmetric Aromatic Side Chain Enables 16.27% Efficiency for Organic Solar Cells

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Chinese Journal of Polymer Science 2023年第7期41卷 1002-1010页

作者： Dong-Xu Li Shu-Fang Li Cheng-Long Wen Chao-Yuan Sun Chang-Zhou Shi xin-xin xia xin-Hui Lu Li-Hui Jiang Jun Yuan Ying-Ping Zou College of Chemistry and Chemical Engineering Central South UniversityChangsha410083China Department of Physics The Chinese University of Hong KongNew TerritoriesHong Kong999077China

In recent years, due to the rapid development of high-performance small molecule acceptor (SMA) materials, the researches on p-type electron donor materials for matching with current efficient SMAs have become important. By means of asymmetric strategies to optimize the energy levels and inter/intramolecular interactions of molecules, we designed and synthesized an asymmetric aromatic side chain quinoxaline-based polymer donor TPQ-0F. Meanwhile, we took advantage of F atom which could form noncovalent interaction and strong electron-withdrawing property, to obtain the optimized quinoxaline-based polymer donors TPQ-1F, TPQ-1Fi and TPQ-2F. Eventually, the binary device based on TPQ-2F achieved an efficient power conversion efficiency (PCE) of 16.27%, which attributed to balanced hole/electron mobilities, less charge carrier recombination, and more favorable aggregation morphology. Our work demonstrates the great potential of asymmetric aromatic side chain quinoxaline-based polymer donors on optimizing the morphology of blending films, improving inter/intramolecular interactions, and subtly tuning energy level, finally for more efficient organic solar cells.

关键词： Asymmetric aromatic side chain Quinoxaline-based polymer donor Fluorine substitution Binary device

The Estimation of Soil Trace Elements Distribution and Soil-Plant-Animal Continuum in Relation to Trace Elements Status of Sheep in Huangcheng Area of Qilian Mountain Grassland,China

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Journal of Integrative Agriculture 2014年第1期13卷 140-147页

作者： WANG Hui LIU Yong-ming QI Zhi-ming WANG Sheng-yi LIU Shi-xiang LI xia WANG Hai-jun WANG xiao-li xia xin-chao ZHU xin-qiang Key Laboratory of Veterinary Pharmaceutics Development Ministry of Agriculture/Key Laboratory of New Animal Drug Project of Gansu Province/Engineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province/Lanzhou Institute of Husbandry and Pharmaceutics SciencesChinese Academy of Agricultural Sciences

The purpose of the present study was to survey contents of trace elements of Cu, Mn, Fe, and Zn in the surface layer （0-20 cm） in the soil, pasture and serum of sheep in Huangcheng area of Qilian mountain grassland, China. Also the soil-plantanimal continuum was analyzed. Soil （n=300）, pasture （n=60）, and blood serum samples from sheep （n=480） were collected from Huangcheng area of Qilian mountain grassland, China. The contents of trace element in the samples were analyzed by atomic absorption spectrophotometer after digestion. The soil trace elements density distribution shows a ladder-like pattern distribution. Equations developed in the present study for prediction of Fe （R2=0.943） and Zn （R2=0.882） had significant R2 values.

关键词： trace elements minerals deficiency soil type Qilian mountain grassland correlation

Inside-out Ostwald ripening： A facile process towards synthesizing anatase TiO2 microspheres for high- efficiency dye-sensitized solar cells

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Nano Research 2016年第7期9卷 1891-1903页

作者： Yong Ding xin xia Wangchao Chen Linhua Hu Li'e Mo Yang Huang Songyuan Dai Beijing Key Laboratory of Novel Thin-Film Solar Cells North China Electric Power University Beijing 102205 China Key Laboratory of Novel Thin-film Solar Cells Institute of Applied Technology Hefei Institutes of Physical Science Chinese Academy ot Sciences Hefei 230031 China

A facile inside-out Ostwald ripening route to the morphology-controlled preparation of TiO2 microspheres is developed. Here, TiO2 hollow microspheres （HM） and solid microspheres （SM） are prepared by adjusting the volume ratio of isopropanol （IPA） to acetylacetone （Acac） in the solvothermal process. During the formation process of HM, precipitation of solid cores, subsequent deposition of outer shells on the surface of cores, and simultaneous core dissolution and shell recrystallizafion are observed, which validate the inside-out Ostwald ripening mechanism. Design and optimization of the properties （pore size, surface area, and trap state） of TiO2 microspheres are vital to the high performance of dye- sensitized solar cells （DSSCs）. The optimized TiO2 rnicrospheres （rHM and rSM） obtained by post-processing on recrystallization, possess large pore sizes, high surface areas and reduced trap states （Ti3~ and oxygen vacancy）, and are thus ideal materials for photovoltaic devices. The power conversion efficiency of DSSCs fabricated using rHM photoanode is 11.22%, which is significantly improved compared with the 10.54% efficiency of the rSM-based DSSC. Our work provides a strategy for synthesizing TiO2 microspheres that simultaneously accommodate different physical properties, in terms of surface area, crystallinity, morphology, and mesoporosity.

关键词： Ostwald ripening dye-sensitized solar cell TiO2 microsphere hollow structure acetylacetone