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Cloning and expression of NS3 cDNA fragment of HCV genome of Hebei isolate in ***

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World Journal of Gastroenterology 1998年第2期4卷 73-76页

作者： Zhu, FL Lu, hy Li, Z Qi, ZT Second Mil Med Univ Dept Microbiol Shanghai 200433 Peoples R China

AIM To obtain greater antigenicity of HCV NS3 protein. METHODS The HCV NS3 cDNA fragment was amplified by reverse transcription polymerase chain reaction from the sera of the HCV infected patients. The DNA sequence was determined by dideoxy mediated chain termination method using T7 polymerase. HCV NS3 protein was expressed in E. coli . RESULTS Sequence analysis indicated that the HCV isolate of this study belongs to HCV Ⅱ; SDS PAGE demonstrated an M r 23800 and an M r 22000 recombinant protein band which amount to 14% and 11% of the total bacterial proteins separately. Western blotting and ELISA showed NS3 protein possessed greater antigenicity. CONCLUSION Recombinant HCV NS3 protein was expressed successfully, which provided the basis for developing HCV diagnostic reagents.

关键词： hepatitis C virus NS3 gene gene expression DNA, viral viral proteins sequence analysis polymerase chain reaction enzyme linked immunosorbent assay Escherichia coli

Electrocatalysis　of　NADH　Oxidation　with　3,4-Dihydroxybenzoic　Acid　Covalently　Bound　to　Self-assembled

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Chemical Research in Chinese Universities 1998年第1期14卷 96-100页

作者： Sun, JJ Fang, HQ Chen, hy Nanjing Univ Dept Chem State Key Lab Coordinat Chem Nanjing 210093 Peoples R China

ＥｌｅｃｔｒｏｃａｔａｌｙｓｉｓｏｆＮＡＤＨＯｘｉｄａｔｉｏｎｗｉｔｈ３，４－ＤｉｈｙｄｒｏｘｙｂｅｎｚｏｉｃＡｃｉｄＣｏｖａｌｅｎｔｌｙＢｏｕｎｄｔｏＳｅｌｆ－ａｓｓｅｍｂｌｅｄＣｙｓｔｅａｍｉｎｅＭｏｎｏｌａｙｅ... 详细信息

ＥｌｅｃｔｒｏｃａｔａｌｙｓｉｓｏｆＮＡＤＨＯｘｉｄａｔｉｏｎｗｉｔｈ３，４－ＤｉｈｙｄｒｏｘｙｂｅｎｚｏｉｃＡｃｉｄＣｏｖａｌｅｎｔｌｙＢｏｕｎｄｔｏＳｅｌｆ－ａｓｓｅｍｂｌｅｄＣｙｓｔｅａｍｉｎｅＭｏｎｏｌａｙｅｒｓｏｎａＧｏｌｄ－ｅｌｅｃｔｒｏ...

关键词： NADH Acid Bound Covalently Dihydroxybenzoic Electrocatalysis Oxidation

Multi-baseline phase unwrapping algorithm for INSAR

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Journal of Systems Engineering and Electronics 2013年第3期24卷 417-425页

作者： Xianming xie School of Information and Communication Guilin University of Electronic Technology

A novel multi-baseline phase unwrapping algorithm based on the unscented particle filter for interferometric synthetic aperture radar （INSAR） technology application is proposed. The proposed method is not constrained by the nonlinearity of the problem and is independent of noise statistics, and performs noise eliminating and phase unwrapping at the same time by combining with an unscented particle filter with a path-following strategy and an omni-directional local phase slope estimator. Results obtained from multi-baseline synthetic data and single-baseline real data show the performance of the proposed method.

关键词： interferometric aperture radar (INSAR) phase un- wrapping unscented particle filter.

Enhanced multi-baseline unscented Kalman filtering phase unwrapping algorithm

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Journal of Systems Engineering and Electronics 2016年第2期27卷 343-351页

作者： Xianming xie School of Information and Communication Guilin University of Electronic Technology

This paper presents an enhanced multi-baseline phase unwrapping algorithm by combining an unscented Kalman filter with an enhanced joint phase gradient estimator based on the amended matrix pencil model, and an optimal path-following strategy based on phase quality estimate function. The enhanced joint phase gradient estimator can accurately and effectively extract the phase gradient information of wrapped pixels from noisy interferograms, which greatly increases the performances of the proposed method. The optimal path-following strategy ensures that the proposed algorithm simultaneously performs noise suppression and phase unwrapping along the pixels with high-reliance to the pixels with low-reliance. Accordingly, the proposed algorithm can be predicted to obtain better results, with respect to some other algorithms, as will be demonstrated by the results obtained from synthetic data.

关键词： multi-baseline phase unwrapping enhanced joint phase gradient estimator unscented Kalman filter

An Enzyme Electrode Based on the Covalently Binding of Horseradish Peroxidase to a Self-assembling Monolayer Modified Gold Electrode

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Chinese Chemical Letters 1998年第2期9卷 157-160页

作者： Sun, JJ Xu, JJ Fang, HQ Chen, hy Nanjing Univ State Key Lab Coordinat Chem Dept Chem Nanjing 210093 Peoples R China

A new procedure for developing high performance enzyme electrode is described. The procedure is based on the covalently binding of horseradish peroxidase to a self-assembling monolayer on a gold electrode. An enhanced catalytic current is observed with addition of hydrogen peroxide into phosphate buffer solution (PBS) in the presence of mediators. The electrode exhibits high sensitivity and a wide linear range to hydrogen peroxide.

关键词： high performance enzyme electrode high sensitivity wide linear range

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论张问陶诗与性灵派

文史杂志 2025年第1期235卷 40-46页

作者：谢桃坊四川省人民政府文史研究馆

清代蜀中第一诗人张问陶,今存诗三千余首。他自15岁作诗,28岁时已呈现独特的艺术个性,其后期的感怀、题画和哲理诗均达于新的境界。他虽与性灵派诗人袁枚有交谊,同宗性灵,论诗亦同,但却具有自己的风格。袁枚诗浮滑纤佻,张问陶诗并无此... 详细信息

清代蜀中第一诗人张问陶,今存诗三千余首。他自15岁作诗,28岁时已呈现独特的艺术个性,其后期的感怀、题画和哲理诗均达于新的境界。他虽与性灵派诗人袁枚有交谊,同宗性灵,论诗亦同,但却具有自己的风格。袁枚诗浮滑纤佻,张问陶诗并无此弊。他情感沉郁,意象新奇,流畅豪放,颇具空灵奇崛的艺术特征,因此在众多清代诗人中能独树一帜。

关键词：张问陶袁枚船山诗性灵派

An　Oligomer　Approach　to　Geometry　Optimization　for　Two　Dimensional　Periodic　Polymers

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Chinese Chemical Letters 1996年第9期7卷 859-861页

作者： Guo, hy Wang, P Li, J Burke, LA BEIJING UNIV CHEM TECHNOL DEPT APPL CHEMBEIJING 100029PEOPLES R CHINA RUTGERS STATE UNIV DEPT CHEMCAMDENNJ 08102

The oligomer approach used in geometry optimization for two dimensional polymers and crystals has been *** optimization for Planar π-conjugated systems,graphite,with the oligomer approach has been performed at AM1 le... 详细信息

关键词： Optimization Two Dimensional Approach An

Skeletal muscle-specific Bambi deletion induces hypertrophy and oxidative switching coupling with adipocyte thermogenesis against metabolic disorders

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Science China Life Sciences 2025年

作者： Xiangping Yao Xudong Mai Ye Tian Yifan Liu Guanghui Jin Ze Li Shujie Chen Xiaoshuang Dai Liujing Huang Zijing Fan Guihua Pan Xiaohan Pan Xiangmin Li Michael C Yu Jia Sun Jingxing Ou Hong Chen Liwei xie College of Life and Health Sciences Guangdong Industry Polytechnic Department of Endocrinology and Metabolism Zhujiang Hospital Southern Medical University State Key Laboratory of Applied Microbiology Southern China Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application Guangdong Open Laboratory of Applied MicrobiologyInstitute of Microbiology Guangdong Academy of Sciences Institute of Aging Research Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics School of Medical Technology Guangdong Medical University Department of Hepatic Surgery and Liver transplantation Center of the Third Affiliated Hospital Organ Transplantation Institute Sun Yat-sen University Organ Transplantation Research Center of Guangdong ProvinceGuangdong province engineering laboratory for transplantation medicine Guangdong Provincial Key Laboratory of Liver Disease Research School of Public Health Xinxiang Medical University BGI Institute of Applied Agriculture BGI-Shenzhen Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic University Department of Biological Sciences State University of New York at Buffalo Department of Internal Medicine Shunde Women and Children's Hospital (Maternity and Child Healthcare Hospital of Shunde Foshan) Guangdong Medical University

Skeletal muscle plays a significant role in both local and systemic energy metabolism. The current investigation aims to explore the role of the Bambi gene in skeletal muscle, focusing on its implications for muscle hypertrophy and systemic metabolism. We hypothesize that skeletal muscle-specific deletion of Bambi induces muscle hypertrophy, improves metabolic performance, and activates thermogenic adipocytes via the reprogramming of progenitor of i WAT, offering potential therapeutic strategies for metabolic syndromes. Leveraging the Chromatin immunoprecipitation(ChIP)-seq and bioinformatics analysis, Bambi gene is shown to be a direct target of HIF2α, which is further confirmed by ChIP-qPCR and promoter luciferase assay. Skeletal muscle-specific Bambi deletion led to significant muscle hypertrophy and improved metabolic parameters, even under high-fat diet conditions. This deletion induced metabolic reprogramming of stromal vascular fractions(SVFs) into thermogenic adipocytes, contributing to systemic metabolic improvements, potentially through the secretory ***, mice with skeletal muscle-specific Bambi deletion demonstrate resistance to high-fat diet-induced metabolic disorders, highlighting a potential therapeutic pathway for metabolic syndrome management. Thus, skeletal muscle-specific deletion of Bambi triggers muscle growth, enhances metabolic performance, and activates thermogenic adipocytes. These findings suggest Bambi as a novel therapeutic target for metabolic syndromes, providing new insights into the interaction between muscle hypertrophy and systemic metabolic improvement. The study underscores the potential of manipulating muscle physiology to regulate whole-body metabolism, offering a novel perspective on treating metabolic disorders.

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植物激素对棉花蕾铃脱落的调控研究进展

作物学报 2025年第1期51卷 1-29页

作者：谢章书谢学方屠小菊刘爱玉董合忠周仲华湖南农业大学农学院/作物生理与分子生物学教育部重点实验室湖南长沙410128 中国热带农业科学院橡胶研究所海南海口571101 山东省农业科学院经济作物研究所山东济南250000

棉花蕾铃脱落是一个普遍存在的现象,有对逆境环境胁迫的主动适应性脱落,也有因品种遗传特性、环境条件、栽培措施以及生物和非生物胁迫被动的受损脱落。蕾铃脱落直接影响着棉花的产量,国内外现有公开报道多集中于20世纪50、60年代关于... 详细信息

棉花蕾铃脱落是一个普遍存在的现象,有对逆境环境胁迫的主动适应性脱落,也有因品种遗传特性、环境条件、栽培措施以及生物和非生物胁迫被动的受损脱落。蕾铃脱落直接影响着棉花的产量,国内外现有公开报道多集中于20世纪50、60年代关于乙烯和脱落酸对棉花蕾铃脱落影响的初步发现。结合其他植物的研究结果来看,蕾铃脱落似乎与生长素、赤霉素和细胞分裂素等促生长类激素的下降以及乙烯和脱落酸等抑制生长类激素的升高密切相关,激素在植物体内不仅介导新陈代谢过程,还协调着与信号通路相关的调控因子,在脱落过程中起着关键作用。本文从植物激素调控入手,综述了近年来包括脱落区形成和作用的分子调控机制以及各激素在棉花蕾铃脱落以及其他植物(生殖)器官脱落过程中的响应和调控机制等。发现目前以棉花蕾铃脱落为对象的研究非常匮乏,且多集中于少数激素对棉花生殖生长影响的有限研究,缺乏对导致蕾铃脱落的深层次机制的探究和解析。因此,未来研究方向应着重于探究棉花蕾铃脱落的遗传基础、发掘新的抗脱落基因资源和培育抗脱落棉花品种,同时加强在以棉花为模型植物下,蕾铃脱落与植物激素调节的关系研究,为提高棉花产量提供理论依据和技术支持。

关键词：棉花植物激素蕾铃脱落研究现状研究方向