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单细胞转录组测序技术在家养动物中的应用

畜牧兽医学报 2024年第8期55卷 3276-3287页

作者：张肖旭李昊冯平捷杨豪李新月吕冉潘章源储明星中国农业科学院北京畜牧兽医研究所畜禽生物育种全国重点实验室北京100193

单细胞转录组测序技术(single cell RNA sequencing, scRNA-seq)能够以高精度分辨率鉴定单个细胞类型和细胞状态,打破普通转录组测序(bulk RNA sequencing, RNA-seq)无法探究目标细胞具体表达特征的困境,在各个领域的探索中起到重要作用... 详细信息

单细胞转录组测序技术(single cell RNA sequencing, scRNA-seq)能够以高精度分辨率鉴定单个细胞类型和细胞状态,打破普通转录组测序(bulk RNA sequencing, RNA-seq)无法探究目标细胞具体表达特征的困境,在各个领域的探索中起到重要作用,目前广泛应用于人类及小鼠发育生物学、肿瘤学、免疫学、复杂疾病、肠道微生物组及临床应用等诸多领域。近年来,scRNA-seq在畜牧业领域也开展了一些开创性的研究并获得了一系列的成果,主要集中在动物繁殖性能、胚胎发育、关键性状解析等方面,但相较于在人类上的应用还较为薄弱,在其他领域的应用也仍有待深入。本文主要综述了scRNA-seq的工作流程及其在家养动物中应用的研究进展,以期为提高scRNA-seq分析效率以及在家养动物中的创新应用提供参考。

关键词：单细胞转录组测序家养动物

High-dimensional proportionality test of two covariance matrices and its application to gene expression data

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Statistical Theory and Related Fields 2022年第2期6卷 161-174页

作者： Long Feng xiaoxu zhang Binghui Liu School of Statistics and Data Science LPMC and KLMDASRNankai UniversityTianjinPeople’s Republic of China School of Mathematics and Statistics and KLAS Northeast Normal UniversityChangchunPeople’s Republic of China

With the development of modern science and technology, more and more high-dimensionaldata appear in the application fields. Since the high dimension can potentially increase the com-plexity of the covariance structure, comparing the covariance matrices among populations isstrongly motivated in high-dimensional data analysis. In this article, we consider the proportion-ality test of two high-dimensional covariance matrices, where the data dimension is potentiallymuch larger than the sample sizes, or even larger than the squares of the sample sizes. We devisea novel high-dimensional spatial rank test that has much-improved power than many exist-ing popular tests, especially for the data generated from some heavy-tailed distributions. Theasymptotic normality of the proposed test statistics is established under the family of ellipticallysymmetric distributions, which is a more general distribution family than the normal distribu-tion family, including numerous commonly used heavy-tailed distributions. Extensive numericalexperiments demonstrate the superiority of the proposed test in terms of both empirical sizeand power. Then, a real data analysis demonstrates the practicability of the proposed test forhigh-dimensional gene expression data.

关键词： Covariance matrices elliptically symmetric distributions high dimension test proportionality spatial rank

耐盐促生菌的分离鉴定及其对设施次生盐渍化土壤的改良效果

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微生物学通报 2024年第9期51卷 3454-3467页

作者：周蕾刘银双牛宏进孙宏勇何艳霞张晓旭张智英黄亚丽河北科技大学环境科学与工程学院河北石家庄050018 中国科学院遗传与发育生物学研究所农业资源研究中心河北石家庄050022 黄骅市自然资源和规划局河北黄骅061100

【背景】土壤次生盐渍化现象非常普遍,严重制约了设施蔬菜产业的可持续发展。【目的】筛选耐盐促生微生物菌株,为设施次生盐渍土壤改良提供菌种资源。【方法】采用稀释涂平板法在含盐量5%的LB培养基上进行盐渍化土壤样品中耐盐菌株的筛... 详细信息

【背景】土壤次生盐渍化现象非常普遍,严重制约了设施蔬菜产业的可持续发展。【目的】筛选耐盐促生微生物菌株,为设施次生盐渍土壤改良提供菌种资源。【方法】采用稀释涂平板法在含盐量5%的LB培养基上进行盐渍化土壤样品中耐盐菌株的筛选;采用浸种法筛选具有促生作用的耐盐菌株;采用形态学、生理生化和16S rRNA基因序列分析对耐盐促生菌进行分类鉴定;采用定性和定量分析法确定菌株的耐盐促生性质;采用盆栽试验评价耐盐促生菌株对设施土壤修复及黄瓜生长的效果。【结果】从采集的37份设施盐渍化土壤样品中分离获得58株耐盐细菌,其中5株菌对黄瓜简化活力指数增长率大于15%,并且在0.3%的NaCl浓度下均对黄瓜幼苗具有一定的促生长作用,但是随着NaCl浓度升高其促生能力下降。经鉴定菌株YQ-1-8为贝莱斯芽孢杆菌(Bacillus velezensis)、菌株SN-1-4为干旱节杆菌(Arthrobacter arilaiti)、菌株3A-2为黄海芽孢杆菌(***)、菌株L1-2为蜡样芽孢杆菌(***)、菌株L3-3为阿氏芽孢杆菌(***),其中菌株3A-2具有解有机磷、产吲哚-3-乙酸(indole-3-acetic acid,IAA)和产胞外多糖的性质。土壤中菌株3A-2浓度为1×10^(7)CFU/g时对黄瓜幼苗有明显的促生作用,茎粗、株高、地上部鲜重、地上部干重和叶绿素分别较对照增加12.39%、14.31%、30.92%、38.46%和9.06%,并且显著增加土壤碱解氮和速效钾含量,降低土壤pH、电导率和全盐含量(P<0.05)。【结论】菌株3A-2兼具耐盐促生作用,能够用于盐渍化土壤的改良,为次生盐渍土壤微生物改良产品的研制提供菌株资源。

关键词：耐盐促生菌促生特性盐渍土壤土壤改良

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芸豆GH3基因家族鉴定及进化与表达分析

西北植物学报 2024年第9期44卷 1433-1444页

作者：张晓旭马媛媛罗新锐秦耀新马天宇王玉萍甘肃农业大学园艺学院兰州730070

【目的】探究芸豆中PvGH3家族成员的特征及在非生物胁迫响应中的作用,为深入研究其基因功能提供参考依据,也为芸豆抗逆新品种的选育提供新的基因资源。【方法】通过生物信息学的方法对芸豆GH3基因家族进行全基因组鉴定,用荧光定量PCR(qR... 详细信息

【目的】探究芸豆中PvGH3家族成员的特征及在非生物胁迫响应中的作用,为深入研究其基因功能提供参考依据,也为芸豆抗逆新品种的选育提供新的基因资源。【方法】通过生物信息学的方法对芸豆GH3基因家族进行全基因组鉴定,用荧光定量PCR(qRT-PCR)检测PvGH3基因在不同逆境胁迫下的表达。【结果】在芸豆中鉴定出19个PvGH3基因,其中17个基因不均匀分布于8条染色体上,其余2个基因定位于scafford上无法精确定位;可分为3个亚族,各亚族间的成员有相似的基因结构和保守基序;各成员间存在3个旁系同源基因对,与拟南芥GH3间有15个直系同源基因对;含有光、激素、胁迫及生长发育相关顺式元件;19个PvGH3在响应干旱(6%PEG)、盐(100 mmol/L NaCl溶液)和低温(4℃)逆境胁迫应答方面均上调表达。【结论】在芸豆中鉴定出19个PvGH3基因,通过qRT-PCR证明这19个PvGH3基因能不同程度地响应非生物胁迫,可为PvGH3的抗逆功能分析提供理论依据。

关键词：芸豆 GH3基因家族生物信息学分析表达分析实时荧光定量PCR

TaGW2L,a GW2-like RING finger E3 ligase,positively regulates heading date in common wheat(Triticum aestivum L.)

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The Crop Journal 2022年第4期10卷 972-979页

作者： Daijing zhang xiaoxu zhang Wu Xu Tingting Hu Jianhui Ma Youfu zhang Jian Hou Chenyang Hao Xueyong zhang Tian Li College of Life Sciences Henan Normal UniversityXinxiang 453007HenanChina Key Laboratory of Crop Gene Resources and Germplasm Enhancement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

RING finger E3 ligases play an important role in regulating plant growth and development by mediating substrate *** this study,we identified TaGW2L,encoding a Grain width and weight2(GW2)-like RING finger E3 ligase,as a novel positive regulator of heading date in wheat(Triticum aestivum L.).TaGW2L exhibited high amino acid sequence similarities with TaGW2 homoeologs,particularly in the conserved RING finger *** analysis indicated that TaGW2L was constitutively expressed in various wheat ***2L showed transactivation activity in yeast and could interact with the ubiquitin-conjugating enzymes E2_(s).An in vitro ubiquitination assay verified that TaGW2L possessed a similar E3 ligase activity to *** of the TaGW2L-7A homoeolog in wheat led to a significantly earlier heading date under both natural conditions and long-day *** analysis revealed that multiple known genes positively regulating wheat heading were significantly upregulated in the TaGW2L-7A-overexpression plants compared with the wild-type ***,our findings shed light on the role of TaGW2L in wheat heading date and provide potential applications of TaGW2L for the adaptation improvement of crops.

关键词： TaGW2L RING finger domain E3 ligase Heading date Triticum aestivum

静息态功能磁共振分析脑小血管病和帕金森病患者脑功能与嗅觉功能的相关性

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中国组织工程研究 2024年第20期28卷 3209-3215页

作者：黄中霞王雨刘雅文张小旭徐丹丹杨艳萍黄明明余晖贵州医科大学医学影像学院贵州省贵阳市550004 贵州医科大学附属医院影像科贵州省贵阳市550004

背景:嗅觉功能异常是许多疾病的早期生物学标志物,但脑小血管病的嗅觉功能异常发生的神经影像机制尚且不清。目的:探索脑小血管病与帕金森病患者嗅觉功能调控的不同神经影像学机制,探讨嗅觉功能评估在脑小血管病患者中的潜在应用价值。... 详细信息

背景:嗅觉功能异常是许多疾病的早期生物学标志物,但脑小血管病的嗅觉功能异常发生的神经影像机制尚且不清。目的:探索脑小血管病与帕金森病患者嗅觉功能调控的不同神经影像学机制,探讨嗅觉功能评估在脑小血管病患者中的潜在应用价值。方法:对80例脑小血管病患者、44例健康对照及29例帕金森病患者行神经心理学及嗅觉测试、高分辨率结构磁共振及静息态功能磁共振扫描。应用DPABI、SPM12、SPSS软件分析脑小血管病、帕金森病患者及健康对照组静息态功能磁共振低频振幅、局部一致性及功能连接的异同,并分析静息态功能磁共振的影像指标与嗅觉和认知评分的相关性。结果与结论:①与对照组相比,脑小血管病、帕金森病组右侧背外侧额上回低频振幅、左侧楔叶局部一致性显著降低;②右侧背外侧额上回及左侧楔叶为种子点,与帕金森病组相比,右侧楔前叶、颞下回、中央前回及背外侧额上回、左侧中央后回及颞下回功能连接在对照、脑小血管病组中显著增强;③左侧楔叶为种子点,与对照组相比,左侧舌回功能连接在脑小血管病、帕金森病组明显减弱;左侧颞中回及右侧中央后回功能连接在对照组较脑小血管病、帕金森病组增强,脑小血管病组较帕金森病组增强;④相关性分析显示,脑小血管病组嗅觉评分与认知评分呈正相关,且左侧楔叶的局部一致性与蒙特利尔认知评估量表评分负相关,帕金森病组中左侧楔叶-左侧颞中回功能连接值与嗅觉识别评分呈正相关,左侧楔叶-左侧中央后回、左侧楔叶-左侧舌回的功能连接值分别与嗅觉识别评分及嗅觉总分呈正相关;⑤提示脑小血管病患者嗅觉功能调控存在不同于帕金森病患者嗅觉障碍的神经影像机制,脑小血管病患者的嗅觉功能与认知功能相关,推测脑小血管病嗅觉功能异常是脑功能异常的继发性改变,而帕金森病嗅觉障碍是由嗅觉相关脑区功能异常直接引起的;脑小血管病患者嗅觉功能评估在预测认知功能方面有潜在应用价值。

关键词：静息态功能磁共振成像脑小血管病嗅觉功能认知功能功能连接

Effects of Electric Potentials on Tribological Properties of SiC/HT200 Pairs in NaOH Solutions

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Journal of Harbin Institute of Technology(New Series) 2016年第3期23卷 75-79页

作者： Pengfei Fan Wenjie Zhai xiaoxu zhang School of Mechatronics Engineering Harbin Institute of Technology

On a self-developed circular-translation polishing machine,the influence of different electric potentials on the friction coefficient and wear properties of SiC/HT200 friction pair,which contacted as one electrode,were studied in NaOH *** results show that the friction coefficient of SiC/HT200 pair is 0.25 under no externally applied potential;but it decreases to 0.23 while applying +3 V polarization potential on the pair;in contrast,it increases to 0.30 when applying-3 V polarization potential on the *** the polished SiC surface under + 3 V potential is much smoother,while there are clear ploughing marks and adhesive region on the polished SiC surface under zero and-3 V potential,*** mixed triboelectrochemical friction model has been proposed and the mechanisms of the electric effects on the tribological properties of SiC/HT200 friction pair in NaOH electrolyte are *** and discussion show that the anodic passivation technique may be used for hastening the running-in process of SiC/Fe friction pairs,as well as for polishing SiC to increase its materials removal efficiency while maintaining good surface quality.

关键词： Silicon carbide tribo-electrochem ical friction and w ear passivation

Tanshinone IIA protects intestinal epithelial cells from ferroptosis through the upregulation of GPX4 and SLC7A11

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BIOCELL 2023年第5期47卷 1107-1115页

作者： HAN WANG YANG SUN xiaoxu zhang XIAOYING WANG YUJUN XIA LISHENG WANG School of Basic Medicine Qingdao UniversityQingdao266071China Laboratory of Molecular Diagnosis and Regenerative Medicine The Affiliated Hospital of Qingdao UniversityQingdao266000China Department of Hematology The Affiliated Hospital of Qingdao UniversityQingdao266000China Beijing Institute of Radiation Medicine Beijing100850China

Background:Inflammatory bowel disease(IBD)is a chronic inflammatory disease of the gastrointestinal *** destruction of the intestinal epithelial barrier is one of the major pathological processes in IBD *** evidence indicated that epithelial cell ferroptosis is linked to IBD and is considered a target ***:RAS-selective lethal 3(RSL3)was used to induce ferroptosis in intestinal epithelial cell line No.6(IEC-6)cells,and cell ferroptosis and the effects of tanshinone IIA(Tan IIA)were determined by cell counting kit-8(CCK-8),reactive oxygen species(ROS)staining,Giemsa staining and transmission electron microscope(TEM).The cell viability of natural product library compounds was determined by *** expression of ferroptosis-related genes were detected by real-time quantitative polymerase chain reaction(RT-qPCR)and western ***:Treatment of IEC-6 cells results in the accumulation of ROS and typical morphological characteristics of ***3 treatment caused rapid cellular cytotoxicity which could be reversed by ferrostatin-1(Fer-1)in IEC-6 *** product library screening revealed that Tan IIA is a potent inhibitor of IEC-6 cell *** IIA could significantly protect the RSL3-induced ferroptosis of IEC-6 ***,the ferroptosis suppressors,glutathione peroxidase 4(GPX4),solute carrier family 7 member 11(SLC7A11),and miR-17-92 were found to be early response genes in RSL3-treated *** of IEC-6 cells with Tan IIA resulted in upregulation of GPX4,SLC7A11,and ***:Our study demonstrated that Tan IIA protects IEC-6 cells from ferroptosis through the upregulation of GPX4,SLC7A11,and *** findings might provide a theoretical grounding for the future application of Tan IIA to treat or prevent IBD.

关键词： Tanshinone IIA GPX4 Ferroptosis Intestinal epithelial cells IBD

Application of Tertiary Amine Cationic Polyacrylamide with High Cationic Degree in Salt-free Dyeing of Reactive Dyes

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Chinese Journal of Chemical Engineering 2010年第6期18卷 1023-1028页

作者： TENG xiaoxu MA Wei zhang Shufen State Key Laboratory of Fine Chemicals Dalian University of Technology Dalian 116024 China

Tertiary amine cationic polyacrylamide with high cationization degree was used as a new cationic agent to pretreat cotton with dip-pad-bake method. The obtained cationic cotton was dyed with reactive dyes in the ab-sence of electrolyte. The effects of the characteristics of the cationic agent and the pretreatment conditions on dye-ability of reactive dye were investigated. The results showed that the fixation and K/S values of the reactive dyes on the cationic cotton were improved compared with those on the untreated one in the presence of salt. Tests on fast-ness properties of the dyed cotton and fabric quality of the pretreated cotton were carried out and the results showed that wash and rub fastness of the salt-free dyeing were both satisfactory. And anti-crease property,tensile and tear strength,and handling of the cationic cotton were also good compared with that of the untreated one.

关键词： tertiary amine cationic polyacrylamide reactive dye cotton fabric salt-free dyeing