检索结果-南通市图书馆

Substitutions of stem-loop subdomains in internal ribosome entry site of Senecavirus A:Impacts on rescue of sequence-modifying viruses

引用

Journal of Integrative Agriculture 2024年第7期23卷 2391-2406页

作者： Qianqian Wang Jie Wang Lei Zhang xiaoxiao duan Lijie Zhu Youming Zhang Yan Li Fuxiao Liu College of Veterinary Medicine Qingdao Agricultural UniversityQingdao 266109China College of Veterinary Medicine Inner Mongolia Agricultural UniversityHuhhot 010018China Shandong New Hope Liuhe Group Co. Ltd.Qingdao 266100China Qingdao Center for Animal Disease Control&Prevention Qingdao 266199China State Key Laboratory of Microbial Technology Shandong UniversityQingdao 266237China

Senecavirus A(SVA)has a positive-sense,single-stranded RNA *** 5´untranslated region harbors an internal ribosome entry site(IRES),comprising 10 larger or smaller stem-loop structures(including a pseudoknot)that have been demonstrated to be well ***,it is still unclear whether each stem-loop subdomain,such as a single stem or loop,is also highly *** clarify this issue in the present study,a set of 29 SVA cDNA clones were constructed by site-directed mutagenesis(SDM)on the *** SDM-modified scenarios included:(1)stem-formed complementary sequences exchanging with each other;(2)loop transversion;(3)loop transition;and(4)point *** cDNA clones were separately transfected into cells for rescuing viable viruses,whereas only four SVAs of interest could be recovered,and were genetically stable during 20 *** progeny grew significantly slower than the other three *** dual-luciferase reporter assay showed that none of the SDM-modified IRESes significantly inhibited the IRES *** previous study indicated that a single motif from any of the ten stem structures,if completely mutated,would cause the failure of virus ***,our present study revealed three stem structures,whose individual complementary sequences could exchange with each other to rescue sequence-modifying ***,one apical loop was demonstrated to have the ability to tolerate its own full-length transition,also having no impact on the recovery of sequence-modifying *** present study suggested that not every stem-loop structure was strictly conserved in its conformation,while the full-length IRES itself was well *** provides a new research direction on interaction between the IRES and many factors.

关键词： SVA HCV IRES HCV-like IRES stem-loop structure cDNA clone virus rescue mutation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Promotional effect of Cu additive for the selective catalytic oxidation of n-butylamine over CeZrO_(x)catalyst

引用

Chinese Chemical Letters 2022年第6期33卷 3065-3072页

作者： Xin Xing Ting Zhao Jie Cheng xiaoxiao duan Wenpeng Li Ganggang Li Zhongshen Zhang Zhengping Hao National Engineering Laboratory for VOCs Pollution Control Material&Technology Research Center for Environmental Material and Pollution Control TechnologyUniversity of Chinese Academy of SciencesBeijing 101408China College of Environmental Science and Engineering Taiyuan University of Science and TechnologyTaiyuan 030024China

The catalytic elimination of nitrogen-containing volatile organic compounds(NVOCs)still encounters bottlenecks in NO_(x)formation and low N_(2)***,a series of Cu-promoted Ce-Zr mixed oxide catalysts were synthesized using a simple precipitation approach,and n-butylamine was adopted as the probe pollutant to evaluate their catalytic *** Ce Cu_(10%)ZrO_(x)catalyst exhibited the best catalytic activity,with 100%n-butylamine conversion and 90%N_(2)selectivity at 250℃.Concurrently,this sample also displayed good water resistance.A detailed characterization of the catalyst was performed through a series of experimental studies and theoretical *** addition of Cu increased the redox property and promoted the production of oxygen vacancies,all of which were favorable for the greatest nbutylamine selective catalytic oxidation *** changes of oxygen vacancies over Ce Cu_(10%)ZrO_(x)in reaction process were studied by in situ Raman ***,in situ diffuse reflectance infrared Fourier transform spectra(DRIFTs)and theoretical calculations were employed to explore the reaction mechanism of n-butylamine selective *** high activity and selectivity of this catalyst confirm the practical feasibility of the selective oxidation of n-butylamine to CO_(2)and N_(2),and the exploration of the reaction mechanism provides new insights into the further design of catalysts.

关键词： N-Butylamine CeCu_(a)ZrO_(x)mixed oxide Selective oxidation Oxygen vacancies Reaction mechanism

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

GreenPhos,a universal method for in-depth measurement of plant phosphoproteomes with high quantitative reproducibility

引用

Molecular Plant 2024年第1期17卷 199-213页

作者： xiaoxiao duan Yuanya Zhang Xiahe Huang Xiao Ma Hui Gao Yan Wang Zhen Xiao Chengcheng Huang Zhongshu Wang Bolong Li Wenqiang Yang Yingchun Wang State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBejing 100101China University of Chinese Academy of Sciences Bejing 100049China Key Laboratory of Photobiology Instute of BotanyChinese Academy of SciencesBejing 100093China

Protein phosphorylation regulates a variety of important cellular and physiological processes in ***-depth profiling of plant phosphoproteomes has been more technically challenging than that of animal *** is largely due to the need to improve protein extraction efficiency from plant cells,which have a dense cell wall,and to minimize sample loss resulting from the stringent sample clean-up steps required for the removal of a large amount of biomolecules interfering with phosphopeptide purification and mass spectrometry *** this end,we developed a method with a streamlined workflow for highly efficient purification of phosphopeptides from tissues of various green organisms including Arabidopsis,rice,tomato,and Chlamydomonas reinhardtii,enabling in-depth identification with high quantitative reproducibility of about 11000 phosphosites,the greatest depth achieved so far with single liquid chromatography-mass spectrometry(LC-MS)runs operated in a data-dependent acquisition(DDA)*** mainstay features of the method are the minimal sample loss achieved through elimination of sample clean-up before protease digestion and of desalting before phosphopeptide enrichment and hence the dramatic increases of time-and *** method,named GreenPhos,combined with single-shot LC-MS,enabled in-depth quantitative identification of Arabidopsis phosphoproteins,including differentially phosphorylated spliceosomal proteins,at multiple time points during salt stress and a number of kinase substrate *** is expected to serve as a universal method for purification of plant phosphopeptides,which,if samples are further fractionated and analyzed by multiple LC-MS runs,could enable measurement of plant phosphoproteomes with an unprecedented depth using a given mass spectrometry technology.

关键词： plant phosphoproteomics phosphopeptides LC–MS Arabidopsis salt stress

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

后电影语境下叙事审美的中国转向

引用

电影评介 2024年第10期 79-84页

作者：段潇潇河北大学外国语学院

伴随着跨媒介传播的施压与数字技术的裹挟,后电影创作呈现出复杂化叙事趋向,使电影创作与接受的主体性呈现超越时空的混沌,进而建构出一种全新的叙事审美。其中叙事技法的发展表征与我国的传统绘画、戏曲、古典诗词、园林艺术等诸多领... 详细信息

伴随着跨媒介传播的施压与数字技术的裹挟,后电影创作呈现出复杂化叙事趋向,使电影创作与接受的主体性呈现超越时空的混沌,进而建构出一种全新的叙事审美。其中叙事技法的发展表征与我国的传统绘画、戏曲、古典诗词、园林艺术等诸多领域的理论经验不谋而合,本文借由迁移及通感理论分析后电影创作趋向,力图还原后电影状态下电影自发寻求“中国方案”的实现路径。

关键词：后电影叙事审美中国审美迁移通感

来源：

维普期刊数据库博看期刊

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Determination of Equilibrium Distribution Coefficients of Impurities in Phosphorus by Vertical Zone-melting Technique

引用

Chinese Journal of Chemical Engineering 2011年第2期19卷 223-226页

作者：任永胜李军段潇潇 School of Chemistry and Chemical Engineering Ningxia University Yinchuan 710021 China School of Chemical Engineering Sichuan University Chengdu 610065 China

The equilibrium distribution coefficients of 12 impurities （As,Fe,Ca,Co,Al,Cr,Cu,Mg,Mn,Ni,Pb,Zn） in phosphorus were obtained by measuring their effective distribution coefficients at zone travel rate of 3,5,10,15,and 20 mm·h-1 in the purification process with vertical zone-melting *** results indicate that the method is *** equilibrium distribution coefficients are below 0.3 except arsenic.

关键词： equilibrium distribution coefficient phosphorus zone-melting purification

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于微流控芯片的细胞共培养技术研究进展

引用

复旦学报（自然科学版） 2020年第2期59卷 245-252页

作者：段肖肖刘思秀复旦大学环境科学与工程系上海200438 复旦大学上海市大气颗粒物污染与防治重点实验室上海200438

细胞共培养技术一般用于研究细胞与细胞间的通讯机制,对揭示多细胞生物生理和病理过程具有重要意义.基于微流控芯片的细胞共培养技术能够模拟原生微环境以进行复杂的代谢和调控,为研究细胞与细胞间通讯提供了新的共培养技术平台,已经广... 详细信息

细胞共培养技术一般用于研究细胞与细胞间的通讯机制,对揭示多细胞生物生理和病理过程具有重要意义.基于微流控芯片的细胞共培养技术能够模拟原生微环境以进行复杂的代谢和调控,为研究细胞与细胞间通讯提供了新的共培养技术平台,已经广泛应用于肿瘤转移及分析、抗癌药物筛选、药物吸收和药物代谢等领域.本文从微流控芯片上细胞共培养系统的设计、共培养系统上的检测以及模型的应用方面进行了总结和展望.

关键词：微流控芯片细胞共培养技术细胞与细胞间通讯

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Detection of Drug Resistance in Escherichia coli Isolated from Animals and ERIC Analysis of Multidrug-resistant Strains

引用

Agricultural Biotechnology 2019年第3期8卷 34-37,176页

作者： xiaoxiao duan Yuqian HE Qizheng BING Shufeng LIU Linghong LIU Weishan CHANG Yan LI Qingdao Animal Disease Prevention and Control Center

The drug resistance of Escherichia coli from animals was detected in Shandong Province in 2016,the gene mapping of Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR) of multidrug-resistant isolates were analyzed,and then the relationship between ERIC-PCR genotyping and drug resistance of highly-resistant *** with multidrug resistance was discussed.A total of 110 *** isolates were separated and identified from diseased swine and avian,and their resistance to 10 kinds of antimicrobials was determined by the agar dilution *** highly-resistant isolates with multidrug resistance were selected to carry on the ERIC-PCR,followed by cluster genetic analysis according to the DNA *** swine-sourced *** isolates possessed serious resistance against several kinds of antimicrobial agents,for example,all isolates were tolerant to florfenicol,doxycycline and ampicillin (100%),but had a relative lower resistance rate to cefotaxime sodium (57.14%).The same situation was observed in the poultry-sourced *** isolates,which had a resistance rate of 95.51% against florfenicol,while the lowest rate of 61.80% appeared on *** of ERTIC showed dispersive fingerprint patterns of highly-resistant and multidrug- resistant isolates which represented a multiple clone resources,and there were certain correlation between the B genotype and the drug-resistant *** indicated that the animal-sourced *** isolates had a high level of drug resistance and were multidrug-resistant,which meant there was severe antibiotic resistance against not only different kinds of antibiotics but also different drugs of the same *** highly-resistant *** isolates with multidrug resistance had no apparent species preference,while their spread and pervasion posed a potential threat to the development of animal husbandry and the public health security.

关键词： Escherichia coli Drug resistance ERIC-PCR Genotype

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

萃取法制取磷酸二氢钠的研究

引用

无机盐工业 2008年第12期40卷 24-26页

作者：段潇潇李军章怡四川大学化学工程学院四川成都610065

研究了以湿法净化磷酸和工业氯化钠为原料，利用有机溶剂萃取法制取磷酸二氢钠的新方法。确定了一种水溶性小、萃取效率高、对盐酸萃取选择性好的萃取剂，并研究了萃取温度、萃取时间、磷酸与氯化钠物质的量比、萃取剂与氯化钠物质的量... 详细信息

研究了以湿法净化磷酸和工业氯化钠为原料，利用有机溶剂萃取法制取磷酸二氢钠的新方法。确定了一种水溶性小、萃取效率高、对盐酸萃取选择性好的萃取剂，并研究了萃取温度、萃取时间、磷酸与氯化钠物质的量比、萃取剂与氯化钠物质的量比、稀释剂加入量等对萃取率和分配比的影响。通过单因素实验确定了较优工艺条件：萃取温度为50℃；萃取时间为20min；磷酸与氯化钠物质的量比为1．0～1．2；萃取剂与氯化钠物质的量比为1．2～1．3；稀释剂加入量为3％-20％（体积分数）；反萃取时间为15min。在此条件下，五氧化二磷收率可达95．64％，产品二水合磷酸二氢钠纯度可达99．5％以上，氯离子质量分数小于0．1％。

关键词：氯化钠磷酸磷酸二氢钠萃取

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

多器官微流控芯片技术及其应用

引用

复旦学报（自然科学版） 2019年第2期58卷 144-150,161页

作者：段肖肖董恒刘思秀复旦大学环境科学与工程系上海200438 复旦大学上海市大气颗粒物污染与防治重点实验室上海200438

器官微流控芯片技术通过模拟人体器官内环境来实现接近体内的体外细胞培养.基于微流控芯片器官模型的发展,构建多器官芯片整合的微流控系统为研究器官之间物质(如药物和外源性污染物)的代谢作用提供了崭新的平台.本文从不同的多细胞共... 详细信息

器官微流控芯片技术通过模拟人体器官内环境来实现接近体内的体外细胞培养.基于微流控芯片器官模型的发展,构建多器官芯片整合的微流控系统为研究器官之间物质(如药物和外源性污染物)的代谢作用提供了崭新的平台.本文从不同的多细胞共培养系统角度介绍了构建多器官微流控芯片模型以及在预测和评估药物代谢及其对人体毒性测试等领域的应用,并对多器官微流控芯片技术的发展进行总结和展望.

关键词：微流控芯片多器官微流控芯片人体器官

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

蠊缨滴虫合并肺吸虫感染2例报告并文献复习

引用

中国基层医药 2023年第6期30卷 806-809页

作者：田云粉段潇潇姜艳云南省第一人民医院、昆明理工大学附属医院儿科昆明650032

目的探讨儿童蠊缨滴虫合并肺吸虫感染的临床表现及诊断、治疗方法,提高儿科医生对该病的认识。方法回顾性分析云南省第一人民医院儿科2020年收治的蠊缨滴虫合并肺吸虫感染患儿2例的临床资料,分析患儿临床表现、诊治过程,并结合文献进行... 详细信息

目的探讨儿童蠊缨滴虫合并肺吸虫感染的临床表现及诊断、治疗方法,提高儿科医生对该病的认识。方法回顾性分析云南省第一人民医院儿科2020年收治的蠊缨滴虫合并肺吸虫感染患儿2例的临床资料,分析患儿临床表现、诊治过程,并结合文献进行复习。结果病例1以消化道症状起病,病例2以头痛、肢体活动障碍起病,2例患儿入院时血常规中嗜酸粒细胞升高,行痰液检查检出活的蠊缨滴虫、肺吸虫虫卵,病例1还查到粪类圆线虫幼虫。经甲硝唑注射液及吡喹酮片治疗,随访均治愈。结论蠊缨滴虫是一种较少见的机会致病性寄生虫,肺吸虫病是一种人兽共患的自然疫源性寄生虫病,两种病原混合感染罕见,期待通过该文拓宽临床医生思路,指导临床。

关键词：滴虫感染蜚蠊目蠊缨滴虫并殖吸虫病原虫感染诊断甲硝唑吡喹酮儿童

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：