检索结果-南通市图书馆

维普期刊数据库

针灸联合中药脐敷治疗儿童单纯性肥胖症的效果

在线全文

学校读者我要写书评

暂无评论

河南医学研究 2024年第2期33卷 359-363页

作者：孔蕊安静黄晓帅许昌市中心医院儿科河南许昌461000

目的探究针灸联合中药脐敷治疗儿童单纯性肥胖症的效果及对体重、糖脂代谢的影响。方法选取2019年10月至2022年10月医院收治的80例单纯性肥胖症患儿,依据随机数字表法分为对照组及观察组。两组患儿均接受常规饮食及运动指导,对照组(40例... 详细信息

目的探究针灸联合中药脐敷治疗儿童单纯性肥胖症的效果及对体重、糖脂代谢的影响。方法选取2019年10月至2022年10月医院收治的80例单纯性肥胖症患儿,依据随机数字表法分为对照组及观察组。两组患儿均接受常规饮食及运动指导,对照组(40例)患者接受针灸治疗,观察组(40例)患儿在对照组基础上接受中药脐敷联合治疗。比较两组患儿疗程结束后疗效,中医证候积分、体重指数(BMI)、体脂率(BFP)、体重、总胆固醇(TC)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)、空腹血糖(FPG)、空腹胰岛素(FINS)水平及不良反应。结果观察组治疗有效率高于对照组(P>0.05)。治疗后两组患儿形体肥胖、倦怠乏力、肢体困重、舌质淡红、苔白腻、脉沉缓评分降低(P<0.05),且观察组低于对照组(P<0.05)。治疗后两组患儿BMI、BFP、体重均降低(P<0.05),且观察组低于对照组(P<0.05)。治疗后两组患儿TC、LDL、FPG、FINS均降低,HDL升高(P<0.05);且观察组TC、LDL、FPG、FINS低于对照组,HDL高于对照组(P<0.05)。结论针灸联合中药脐敷治疗儿童单纯性肥胖症效果确切,可有效降低患儿体重,正性调节糖脂代谢水平,具有良好的安全性。

关键词：儿童单纯性肥胖症针灸中药脐敷体重糖脂代谢

维普期刊数据库博看期刊

The transcriptome analysis of cleft lip/palate-related PTCH1 variants in GMSM-K cells show carcinogenic potential

在线全文

学校读者我要写书评

暂无评论

BIOCELL 2023年第1期47卷 205-214页

作者： MINGZHAO LI QIAN ZHANG WENBIN huang SHIYING ZHANG NAN JIANG xiaoshuai huang FENG CHEN Department of Orthodontics Peking University School and Hospital of StomatologyBeijingChina Central Laboratory Peking University School and Hospital of StomatologyBeijingChina Biomedical Engineering Department Peking UniversityBeijingChina

Cancer progression involves the sonic hedgehog(SHH)pathway,in which the receptor PTCH1 actives the downstream *** of PTCH1 can lead to nevoid basal cell carcinoma Syndrome(NBCCs)including neoplastic disease and congenital *** evaluate the relationship between PTCH1 and cancer,we applied the CRISPR/Cas9 system to knock out PTCH1 in oral nontumorous epithelial cells(GMSM-K).Then we screened six PTCH1 variants associated with cleft lip/palate(CL/P),one of the congenital disorders in NBCCs,and generated PTCH1 variant and wild-type recombinant PTCH1^(−/−)GMSM-K cell *** sequencing was conducted in these cell *** results revealed that differentially expressed genes(DEGs)in PTCH1^(−/−)GMSM-K were enriched in extracellular compartments,contributing epithelial diseases by pathway enrichment ***-PCR confirmed that KRT34,KRT81,KRT86,PDGFB,and WNT10B genes,associated with extracellular compartments were highly expressed in PTCH1^(−/−).The Kyoto Encyclopedia of Genes and Genomes analysis also suggested that DEGs are closely related to focal adhesion,transcriptional misregulation,and proteoglycans in breast and gastric *** analysis of samples revealed that the CL/P-associated PTCH1 variants A443G and V908G are potentially *** findings provide new insights into the carcinogenic potential of PTCH1 dysfunction.

关键词： CRISPR/Cas9 PTCH1 variant Carcinogenic potential Transcriptome sequencing

A protocol for structured illumination microscopy with minimal reconstruction artifacts

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Biophysics Reports 2019年第2期5卷 80-90页

作者： Junchao Fan xiaoshuai huang Liuju Li Shan Tan Liangyi Chen Key Laboratory of Image Processing and Intelligent Control of Ministry of Education of China School of Automation Huazhong University of Science and Technology State Key Laboratory of Membrane Biology Beijing Key Laboratory of Cardiometabolic Molecular MedicineInstitute of Molecular Medicine Peking University

The imaging rate of structured illumination microscopy(SIM) reached 188 Hz recently. As the exposure time decreases, the camera detects fewer virtual photons, while the noise level remains the same. As a result, the signal-to-noise ratio(SNR) decreases sharply. Furthermore, the SNR decreases further because of photobleaching and phototoxicity. This decreased quality of SIM raw data may lead to surprising artifacts with various causes, which may confuse a new user of SIM microscopy. We summarize three significant possible sources of severe artifacts in reconstructed super-resolution(SR)images. Ultrafast motion of a biological sample or an uneven illumination pattern is the most difficult to be identified. The estimated parameter could also be incorrect, leading to artifact of regular ***, reconstruction with the Wiener method generates stochastic artifacts due to the amplification of noise during the deconvolution process. To deal with these problems, we have established a protocol to reconstruct ultrafast SIM raw data obtained in low SNR conditions. First, we checked the quality of the raw data with the Image J plugin SIMcheck before reconstruction. Then, a modified parameter estimation method was used to improve the precision of the parameters. Finally, an iterative algorithm was used for SIM reconstruction under low signal-to-noise ratio conditions. This procedure effectively suppressed the artifacts in the super-resolution images reconstructed from raw data of low signal-to-noise ratio.

关键词： Ultrafast SIM Low signal-to-noise ratio Reconstruction

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

番石榴多糖化学与生物活性研究进展

食品研究与开发 2024年第10期45卷 209-216页

作者：许凯博梁钰婵黄小帅芦昕蒂秦赫婕李莉娅东北大学生命科学与健康学院辽宁沈阳110169 辽宁省生物资源开发利用重点实验室辽宁沈阳110169

番石榴(Psidium guajava)为桃金娘科植物,在全球热带及亚热带地区广泛种植。番石榴具有良好的食用和药用价值,长期被用于腹泻、糖尿病等疾病的辅助治疗。多糖是番石榴中的重要活性成分,具有抗氧化、抗肿瘤、抗炎、调节免疫、降血糖、改... 详细信息

番石榴(Psidium guajava)为桃金娘科植物,在全球热带及亚热带地区广泛种植。番石榴具有良好的食用和药用价值,长期被用于腹泻、糖尿病等疾病的辅助治疗。多糖是番石榴中的重要活性成分,具有抗氧化、抗肿瘤、抗炎、调节免疫、降血糖、改善肠道微生态等作用。该文对已报道的番石榴多糖提取方法、结构表征及生物活性相关研究进行梳理与总结,以期为番石榴及其多糖类成分在食品及医药领域的进一步开发利用提供参考和借鉴。

关键词：番石榴多糖提取工艺结构表征生物活性

维普期刊数据库

Super-resolution fluorescence-assisted diffraction computational tomography reveals the threedimensional landscape of the cellular organelle interactome

在线全文

学校读者我要写书评

暂无评论

Light(Science & Applications) 2020年第1期9卷 1889-1903页

作者： Dashan Dong xiaoshuai huang Liuju Li Heng Mao Yanquan Mo Guangyi Zhang Zhe Zhang Jiayu Shen Wei Liu Zeming Wu Guanghui Liu Yanmei Liu Hong Yang Qihuang Gong Kebin Shi Liangyi Chen State Key Laboratory for Mesoscopic Physics and Frontiers Science Center for Nano-optoelectronics School of PhysicsPeking UniversityBeijing 100871China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuanShanxi 030006China State Key Laboratory of Membrane Biology Beijing Key Laboratory of Cardiometabolic Molecular MedicineInstitute of Molecular MedicinePeking UniversityBeijing 100871China School of Mathematical Sciences Peking UniversityBeijing 100871China School of Software and Microelectronics Peking UniversityBeijing 100871China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China National Laboratory of Biomacromolecules Chinese Academy of Sciences Center for Excellence in BiomacromoleculesInstitute of BiophysicsBeijing 100101China Institute for Brain Research and Rehabilitation(IBRR) Guangdong Key Laboratory of Mental Health and Cognitive ScienceSouth China Normal UniversityGuangzhouChina Collaborative Innovation Center of Quantum Matter Peking UniversityBeijing 100871China PKU-IDG/McGovern Institute for Brain Research Beijing 100871China

The emergence of super-resolution(SR)fluorescence microscopy has rejuvenated the search for new cellular ***,SR fluorescence microscopy achieves high contrast at the expense of a holistic view of the interacting partners and surrounding ***,we developed SR fluorescence-assisted diffraction computational tomography(SR-FACT),which combines label-free three-dimensional optical diffraction tomography(ODT)with two-dimensional fluorescence Hessian structured illumination *** ODT module is capable of resolving the mitochondria,lipid droplets,the nuclear membrane,chromosomes,the tubular endoplasmic reticulum,and *** dual-mode correlated live-cell imaging for a prolonged period of time,we observed novel subcellular structures named dark-vacuole bodies,the majority of which originate from densely populated perinuclear regions,and intensively interact with organelles such as the mitochondria and the nuclear membrane before ultimately collapsing into the plasma *** work demonstrates the unique capabilities of SR-FACT,which suggests its wide applicability in cell biology in general.

关键词： landscape illumination partner

Structured illumination microscopy imaging of mitochondria in living cells

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Structured illumination microscopy imaging of mitochondria i...

Two sex pheromone receptors for sexual communication in the American cockroach

第三届中国生物物理学会代谢生物学分会学术研讨会

作者： xiaoshuai huang Junchao Fan Liuju Li Shan Tan Liangyi Chen Institute of Molecular Medicine Peking University School of Automation Huazhong University of Science and Technology

*** The mitochondrion is an essential organelle to produce energy in *** is challenging for fluorescence microscopy due to the highly folded inner membrane with topologically complicated *** illumination microscopy(SIM) with our proposed reconstruction method is the most suitable fluorescence microscopy to visualize mitochondrial inner membrane dynamics in living cells owing to a less photodamage and a higher *** The Hessian-SIM can retain the details and suppress the artifacts in the ***.1 shows the mitochondrial inner membrane in living COS-7 cells labeled with MitoTracker Green FM under wide field(a) and 2 D-SIM(b).It provides a detailed view on the inner membrane architecture in *** We proposed a modified parameter estimation method and Hessian-SIM microscopy,which can visualize vivid dynamics of mitochondrial cristae structures during fusion,fission and inter-cristae remodeling in living cells.

关键词： Structured illumination microscopy Fast Long-term

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2024年第7期67卷 1455-1467页

作者： Na Li Renke Dong Huanchao Zeng Yan Zhang Run huang Wei Liu Fengming Cao Jincong Yu Mingtao Liao Jingyou Chen Wenlei Zhang Zejian huang Jiahui Wang Li Li Shen Zhu Danyan huang Zining Li xiaoshuai Zhang Dongwei Yuan Nan Chen Yongliang Fan Guirong Wang Coby Schal Yufeng Pan Sheng Li Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhou 510631China Guangmeiyuan R&D Center Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied TechnologySouth China Normal UniversityMeizhou 514589China Lingnan Guangdong Laboratory of Modern Agriculture Genome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518000China State Key Laboratory of Crop Stress Biology for Arid Areas and Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of AgricultureNorthwest A&F UniversityYangling 712100China Department of Entomology and Plant Pathology North Carolina State UniversityRaleigh 27695USA The Key Laboratory of Developmental Genes and Human Disease School of Life Science and TechnologySoutheast UniversityNanjing 210096China

Volatile sex pheromones are vital for sexual communication between males and *** of the American cockroach,Periplaneta americana,produce and emit two sex pheromone components,periplanone-A(PA)and periplanone-B(PB).Although PB is the major sex attractant and can attract males,how it interacts with PA in regulating sexual behaviors is still *** this study,we found that in male cockroaches,PA counteracted PB *** identified two odorant receptors(ORs),OR53 and OR100,as PB/PA and PA receptors,***53 and OR100 were predominantly expressed in the antennae of sexually mature males,and their expression levels were regulated by the sex differentiation pathway and nutrition-responsive *** localization of OR53 and OR100 in male antennae further revealed that two types of sensilla coordinate a complex two-pheromone-two-receptor pathway in regulating cockroach sexual *** findings indicate distinct functions of the two sex pheromone components,identify their receptors and possible regulatory mechanisms underlying the male-specific and age-dependent sexual behaviors,and can guide novel strategies for pest management.

关键词： sex pheromone odorant receptor sexual attraction sex differentiation cockroach

维普期刊数据库

Myosin ⅡA-mediated endothelial exocytosis is critical for vascular homeostasis

在线全文

学校读者我要写书评

暂无评论

Myosin ⅡA-mediated endothelial exocytosis is critical for v...

Quantitatively mapping local quality of super-resolution microscopy by rolling Fourier ring correlation

中国生理学会基质生物学专业委员会第二次全国基质生物学学术会议

作者： Pin Li 魏国琴 Yang Cao Qiuping Deng xiaoshuai huang Yingqing Huo Liangyi Chen Jincai Luo Laboratory of Vascular Biology Institute of Molecular MedicinePeking University 北京大学分子医学研究所 Beijing Key Laboratory of Cardiometabolic Molecular Medicine Institute of Molecular MedicinePeking University

The exocytosis of vascular endothelial cells is critical to maintaining the homeostasis of the blood ***-Palade bodies(WPBs)are endothelial-specific granules containing various bioactive molecules,including von Willebrand factor(VWF)and P-selectin,which regulate both the angiogenesis and the interaction with surrounding cells or *** previous work revealed that the focal adhesion(FA)protein zyxin promotes the

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Light(Science & Applications) 2023年第12期12卷 2826-2844页

作者： Weisong Zhao xiaoshuai huang Jianyu Yang Liying Qu Guohua Qiu Yue Zhao Xinwei Wang Deer Su Xumin Ding Heng Mao Yaming Jiu Ying Hu Jiubin Tan Shiqun Zhao Leiting Pan Liangyi Chen Haoyu Li Innovation Photonics and Imaging Center School of Instrumentation Science and EngineeringHarbin Institute of TechnologyHarbinChina Key Laboratory of Ultra-Precision Intelligent Instrumentation of Ministry of Industry and Information Technology Harbin Institute of TechnologyHarbinChina Biomedical Engineering Department International Cancer InstitutePeking University Cancer Hospital and InstituteHealth Science CenterPeking UniversityBeijingChina The Key Laboratory of Weak-Light Nonlinear Photonics of Education Ministry School of Physics and TEDA Institute of Applied PhysicsFrontiers Science Center for Cell ResponsesNankai UniversityTianjinChina State Key Laboratory of Membrane Biology Beijing Key Laboratory of Cardiometabolic Molecular MedicineInstitute of Molecular MedicineNational Biomedical Imaging CenterSchool of Future TechnologyPeking UniversityBeijingChina Department of Control Science and Engineering Harbin Institute of TechnologyHarbinChina School of Mathematical Sciences Peking UniversityBeijingChina Unit of Cell Biology and Imaging Study of Pathogen Host Interaction The Center for MicrobesDevelopment and HealthKey Laboratory of Molecular Virology and ImmunologyShanghai Institute of Immunity and InfectionChinese Academy of SciencesShanghaiChina School of Life Science and Technology Harbin Institute of TechnologyHarbinChina PKU-IDG/McGovern Institute for Brain Research BeijingChina Beijing Academy of Artificial Intelligence BeijingChina Frontiers Science Center for Matter Behave in Space Environment Harbin Institute of TechnologyHarbinChina Key Laboratory of Micro-Systems and Micro-Structures Manufacturing of Ministry of Education Harbin Institute of TechnologyHarbinChina

In fluorescence microscopy,computational algorithms have been developed to suppress noise,enhance contrast,and even enable super-resolution(SR).However,the local quality of the images may vary on multiple scales,and these differences can lead to *** mapping methods fail to finely estimate the local quality,challenging to associate the SR scale ***,we develop a rolling Fourier ring correlation(rFRC)method to evaluate the reconstruction uncertainties down to SR *** visually pinpoint regions with low reliability,a filtered rFRC is combined with a modified resolution-scaled error map(RSM),offering a comprehensive and concise map for further *** demonstrate their performances on various SR imaging modalities,and the resulting quantitative maps enable better SR images integrated from different ***,we expect that our framework can become a routinely used tool for biologists in assessing their image datasets in general and inspire further advances in the rapidly developing field of computational imaging.

关键词： resolution local enable