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Chinese Chemical Letters 2019年第12期30卷 2216-2220页

作者： xiaoman lei Menghan You Fei Pan Min Liu Peng Yang Dongsheng Xia Qiang Li Yanting Wang Jie Fu School of Environmental Engineering Wuhan Textile UniversityEngineering Research Centre for Clean Production of Textile Dyeing and PrintingMinistry of EducationWuhan 430073China Department of Environmental Science&Engineering Fudan UniversityShanghai 200438China

Recently,heterogeneous activation of peroxymonosulfate(PMS) to oxidatively degrade organic pollutants has been a *** the present work,copper ferrite-graphite oxide hybrid(CuFe2 O4@GO)was prepared and used as catalyst to activate PMS for degradation of methylene blue(MB) in aqueous solution.A high degradation efficiency(93.3%) was achieved at the experimental conditions of20 mg/L MB,200 mg/L CuFe2 O4@GO,0.8 mmol/L PMS,and 25℃***,CuFe2 O4@GO showed an excellent reusability and *** effects of various operational parameters including pollutant type,solution pH,catalyst dosage,PMS dosage,pollutant concentration,temperature,natural organic matter(NOM),and inorganic anions on the catalytic degradation process were comprehensively investigated and *** further mechanistic study revealed the Cu(Ⅱ)/Cu(Ⅰ) redox couple on CuFe2 O4@GO played the dominant role in PMS activation,where both hydroxyl and sulfate radicals were generated and proceeded the degradation of *** general,CuFe2 O4@GO is a promising heterocatalyst for PMS-based advanced oxidation processes(AOPs) in wastewater treatment.

关键词： CuFe2O4@GO Peroxymonosulfate Methylene blue Sulfate radical Advanced oxidation processes

Dietary yeast extract prevents alcoholic liver disease in mice via correcting gut dysbiosis and improving intestinal barrier function

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Food Science and Human Wellness 2024年

作者： Hao Han lei Tian Yajing Dong Yanyang Han xiaoman Wang Qian Cheng Haibo Zhang Yan Zhang Department of Nutrition and Food Hygiene， School of Public Health， Shanxi Medical University Hubei Provincial Key Laboratory of Yeast Function Research Center of Nutrition and Health Food Yichang School of Health Science and Engineering， Shanghai Engineering Research Center of Food Microbiology， University of Shanghai for Science and Technology

Alcoholic liver disease (ALD) is one of the major global public health problems. Yeast extract (YE)， a product prepared from yeast， has been proven to have antioxidant and anti-inflammatory properties. However， the potential role of YE in the prevention of ALD remains unclear. The present study aimed to investigate the protective effects of YE on ALD and explore the underlying mechanism based on gut microbiota. The result showed that YE supplementation significantly ameliorated chronic alcohol exposure-induced liver injury in mice. In addition， YE counteracted alcohol-induced gut dysbiosis， intestinal barrier dysfunction， lipopolysaccharide (LPS) leakage-induced inflammatory response in the liver. Moreover， microbiota depletion by a broad-spectrum antibiotic was sufficient to block the protective effect of YE on ALD， indicating the contribution of gut dysbiosis modulation to the hepatoprotective role of YE. Furthermore， we demonstrated the causal relationship between gut microbiota and hepatoprotective effects of YE with the fecal microbiota transplantation (FMT) experiment. Compared with the ALD-FMT mice， gut dysbiosis， intestinal barrier dysfunction， LPS/TLR4 signaling pathway activation， and liver inflammatory response were significantly improved in the YE-FMT mice. Together， our findings highlight that dietary YE protects against ALD through gut dysbiosis correction.

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OsPRMT6a-mediated arginine methylation of OsJAZ1 regulates jasmonate signaling and spikelet development in rice

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Molecular Plant 2024年第6期17卷 900-919页

作者： Kun Dong Fuqing Wu Siqi Cheng Shuai Li Feng Zhang Xinxin Xing Xin Jin Sheng Luo Miao Feng Rong Miao Yanqi Chang Shuang Zhang xiaoman You Peiran Wang Xin Zhang Cailin lei Yulong Ren Shanshan Zhu Xiuping Guo Chuanyin Wu Dong-lei Yang Qibing Lin Zhijun Cheng Jianmin Wan State Key Laboratory of Crop Gene Resources and Breeding National Key Facility for Crop Gene Resources and Genetic ImprovementInstitute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China State Key Laboratory for Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing 210095China

Although both protein arginine methylation(PRMT)and jasmonate(JA)signaling are crucial for regulating plant development,the relationship between these processes in the control of spikelet development remains *** this study,we used the CRISPR/Cas9 technology to generate two OsPRMT6a loss-of-function mutants that exhibit various abnormal spikelet ***,we found that OsPRMT6a can methylate arginine residues in JA signal repressors OsJAZ1 and *** showed that arginine methylation of OsJAZ1 enhances the binding affinity of OsJAZ1 with the JA receptors OsCOI1a and OsCOI1b in the presence of JAs,thereby promoting the ubiquitination of OsJAZ1 by the SCF^(OsCOI1a/OsCOI1b) complex and degradation via the 26S *** process ultimately releases OsMYC2,a core transcriptional regulator in the JA signaling pathway,to activate or repress JA-responsive genes,thereby maintaining normal plant(spikelet)***,in the osprmt6a-1 mutant,reduced arginine methylation of OsJAZ1 impaires the interaction between OsJAZ1 and OsCOI1a/OsCOI1b in the presence of *** a result,OsJAZ1 proteins become more stable,repressing JA responses,thus causing the formation of abnormal spikelet ***,we discovered that JA signaling reduces the OsPRMT6a mRNA level in an OsMYC2-dependent manner,thereby establishing a negative feedback loop to balance JA *** further found that OsPRMT6a-mediated arginine methylation of OsJAZ1 likely serves as a switch to tune JA signaling to maintain normal spikelet development under harsh environmental conditions such as high ***,our study establishes a direct molecular link between arginine methylation and JA signaling in rice.

关键词： protein arginine methylation jasmonate signaling negative feedback loop spikelet development adaptation to heat stress rice

高职扩招下“多元化生源结构”带来的机遇与挑战及应对策略探析

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装备制造与教育 2021年第3期35卷 20-23,27页

作者：张伟雷晓漫苗景国四川工程职业技术学院四川德阳618000 达州职业技术学院四川达州635000

高职院校扩招百万政策是国家的战略举措,对我国高职教育和高职院校都带来了深远影响,本文从详细的学情分析入手,深入探讨了多元化生源结构给高职院校带来的机遇与挑战及应对政策,文章提出:面对各种挑战,高职院校应以转变职业教育传统思... 详细信息

高职院校扩招百万政策是国家的战略举措,对我国高职教育和高职院校都带来了深远影响,本文从详细的学情分析入手,深入探讨了多元化生源结构给高职院校带来的机遇与挑战及应对政策,文章提出:面对各种挑战,高职院校应以转变职业教育传统思维,加强顶层设计为前提,以创新人才培养模式为关键,以完善教学管理制度为突破,以质量评估为抓手,实施分类培养、分层教学。因材施教、因人施策,实现“人人皆可成才、人人皆能成才”。服务国家“保就业,促发展”的战略和社会经济的发展,同时促进高职教育迈向更高台阶。

关键词：高职院校扩招百万生源结构多元化机遇与挑战

Polymer-chlorella cells conjugating with aggregation-induced functionality switch towards hydrogen evolution

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Science China(Technological Sciences) 2020年第8期63卷 1416-1425页

作者： WANG ShengLiang XU ZhiJun LIN Song LIU xiaoman WANG lei HUANG Xin MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage School of Chemistry and Chemical EngineeringHarbin Institute of TechnologyHarbin 150001China

The manipulation of physicochemical and biological properties of the encapsulated cells by cell surface engineering technique is now attracting more and more researchers’*** this study,we showed a mild and highly efficient way to build a type of polymer-living cell conjugates based on photoinduced electron transfer-reversible addition-fragmentation chain-transfer polymerization(PET-RAFT).The polymerization could proceed at room temperature under the blue light initiating and showed very less influence on the activity of the living ***,the constructed positively charged polymer-living cell conjugates could aggregate with the negatively charged native Chlorella pyrenoidosa(CP)cell to form a microscale aggregate which then allowed a functionality switching of the encapsulated CP cells from the normal photosynthetic O2 towards H2 production with an average rate of 0.26μmol H2h-^1(mg chlorophyll)-^1 for 42 *** is then anticipated that the method we show could contribute an effective way for generating different functionalized living cell-polymer hybrid structures on demand,and the demonstrated aggregation-induced functionality switching of the CP cells is also anticipated to inspire more applications of the cell-based materials towards biology green energy.

关键词： cell surface engineering reversible addition-fragmentation chain-transfer polymerization self-assembly biology green energy

A pH Self-Monitoring Heterogeneous Multicompartmental Proteinosome with Spatiotemporal Regulation of Insulin Transportation

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Chinese Journal of Chemistry 2021年第12期39卷 3386-3392页

作者： Ping Wen Xueyi Wang Haixu Chen Dietmar Appelhans xiaoman Liu lei Wang Xin Huang MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage School of Chemistry and Chemical EngineeringHarbin Institute of Technology(HIT)HarbinHeilongjiang 150001China Dongguan Hospital of Southern Medical University Southern Medical UniversityDongguanGuangdong 523059China leibniz-lnstitut fur Polymerforschung Dresden e.V. Hohe Strafe 6Dresden 01069Germany

Inspired by the intricate eukaryotic cell structure,the spontaneously assembling multicompartments capable of spatiotemporally regulated biomacromolecules transportation is still a ***,a heterogeneous proteinosome-based multicompartment was designed and constructed by combining aqueous two-phase system and Pickering emulsion *** loading PEGylated insulin with boronic acid group into the multicompartment and glucose oxidase(GOx)into the innermost microgel domain,a short-or long-term transportation pathway of the loaded insulin was constructed by responding to the concentration of ***,the diminution of dynamic boronic ester bonding,the hindrance of polyethylene oxide micro-chamber,electrostatic interaction and the swelling behavior of hydrogel triggered by GOx/glucose reaction resulted in spatiotemporally controlled multi-pathway transportation mode with the releasing of insulin loaded inside the innermost microgel at high concentration of glucose(10 mg·mL^(-1)).Specially,by incorporating rhodamine B and fluorescein labelled BSA into the multicompartment,the release procedure of the loaded insulin can be monitored by the fluorescence ***,a multimode microcompartment is constructed which is then expected to provide a promising platform for further therapeutic protein,gene and drug delivery,as well as towards the design of complicated cell biomimetics.

关键词： Multicompartment Vesicles Sensors Spatiotemporal regulation Drug delivery

Proliferation-mediated asymmetric nanoencapsulation of singlecell and motility differentiation

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Science China(Technological Sciences) 2023年第7期66卷 2134-2146页

作者： WANG ShengLiang XU ZhiJun CHEN HaiXu WANG XiaoLiang LIN Song WANG lei LIU xiaoman HUANG Xin MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage School of Chemistry and Chemical EngineeringHarbin Institute of TechnologyHarbin 150001China

The biointerface engineering of living cells by creating an abiotic shell has important implications for endowing cells with exogenous properties with improved cellular behavior,which then boosts the development of the emerging field of living cell hybrid ***,we develop a way to perform active nanoencapsulation of single cell,which then endows the encapsulated cells with motion ability that they do not inherently *** emerging motion characteristics of the encapsulated cells could be self-regulated in terms of both the motion velocity and orbits by different proliferation ***,by taking advantage of the emergence of differentiated moving abilities,we achieve the self-sorting between mother cells and daughter cells in a proliferated Saccharomyces cerevisiae cell ***,it is anticipated that our highlighted study could not only serve as a new technique in the field of single-cell biology analysis and sorting such as in studying the aging process in Saccharomyces cerevisiae,but also open up opportunities to manipulate cell functionality by creating biohybrid materials to fill the gap between biological systems and engineering abiotic materials.

关键词： biointerface engineering micromotors self-propulsion self-sorting urease

Caldicellulosiruptor owensensis木聚糖酶的融合表达、酶学性质研究及在面包烘焙中的应用

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食品与发酵工业 2024年第14期50卷 120-125页

作者：严鹤松段坦雷晶晶王佳张晓曼李玥舒玉凤李婵娟武汉设计工程学院食品与生物科技学院湖北武汉430205 湖北轻工职业技术学院轻化工程学院湖北武汉430070

木聚糖酶是一种新型的安全无毒面粉改良剂,能有效改善面团性能和烘焙质量。为了挖掘新的木聚糖酶资源,并评估其在面包烘焙中的应用,该研究在大肠杆菌中融合Sumo标签重组表达了来自嗜热微生物Caldicellulosiruptor owensensis的木聚糖酶... 详细信息

木聚糖酶是一种新型的安全无毒面粉改良剂,能有效改善面团性能和烘焙质量。为了挖掘新的木聚糖酶资源,并评估其在面包烘焙中的应用,该研究在大肠杆菌中融合Sumo标签重组表达了来自嗜热微生物Caldicellulosiruptor owensensis的木聚糖酶,并对融合木聚糖酶SumoXyn的酶学性质和对面包烘焙的品质影响进行了初步研究。大肠杆菌能高效表达***的木聚糖酶,且仅需通过75℃加热细胞破碎液30 min这种简单操作即可得到纯度较高的融合木聚糖酶SumoXyn,微量差示扫描荧光分析发现该酶热变性中点温度值为83.5℃。对其酶学性质研究发现该酶最适pH值为9.0,但在pH 5~10时仍有60%以上的活性。在pH 4~10的缓冲液中处理24 h,SumoXyn仍有80%以上的残余活性;该酶在80℃时酶活性最高,但在100℃仍有50%的酶活性,75℃加热1 h后仍有约75%的残余活性,表明SumoXyn具有较好的pH稳定性和热稳定性。以榉木木聚糖为底物,测得其v max为2354.52μmol/(min·mg),K m为2.26 mg/mL,K cat为214.68 s^(-1)。以200 g高筋全麦面粉为原料制作面包,研究添加量分别为3、6、9 mg的SumoXyn对全麦面包品质的影响,发现随着SumoXyn添加量的增加,面包比容和弹性增加、硬度和咀嚼性降低,全麦面包品质得到显著改善,说明SumoXyn是一种具有潜力的面粉改良剂。

关键词：木聚糖酶酶学性质烘焙面包品质

Structural Basis of Glycan Recognition in Globally Predominant Human P[8]Rotavirus

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Virologica Sinica 2020年第2期35卷 156-170页

作者： xiaoman Sun lei Dang Dandi Li Jianxun Qi Mengxuan Wang Wengang Chai Qing Zhang Hong Wang Ruixia Bai Ming Tan Zhaojun Duan National Health Commission Key Laboratory for Medical Virology and Viral Diseases Beijing 102206China National Institute for Viral Disease Control and Prevention China CDC.Beijing 102206China Inner Mongolia Medical University Huhehaote 010059China Institute of Microbiology Chinese Academy of SciencesBeijing 100101China Glycosciences Laboratory Department of MedicineImperial College LondonLondonUK Division of Infectious Diseases University of Cincinnati College of MedicineCincinnatiOHUSA

Rotavirus(RV)causes acute gastroenteritis in infants and children *** studies showed that glycans such as histo-blood group antigens(HBGAs)function as cell attachment factors affecting RV host susceptibility and prevalence.P[8]is the predominant RV genotype in humans,but the structural basis of how P[8]RVs interact with glycan ligands remains *** this study,we characterized the interactions between P[8]VP8~*s and glycans which showed that VP8~*,the RV glycan binding domain,recognized both mucin core 2 and H type 1 antigens according to the ELISA-based oligosaccharide binding ***,we determined the structural basis of P[8]RV-glycans interaction from the crystal structures of a Rotateq P[8]VP8~*in complex with core 2 and H type 1 glycans at 1.82.3?,respectively,revealing a common binding pocket and similar binding *** and sequence analysis demonstrated that the glycan binding site is conserved among RVs in the P[Ⅱ]genogroup,while genotype-specific amino acid variations determined different glycan binding *** data elucidated the detailed structural basis of the interactions between human P[8]RVs and different host glycan factors,shedding light on RV infection,epidemiology,and development of anti-viral agents.

关键词： Rotavirus(RV)·P Glycan binding specificity VPS*structure Mucin core 2 Lacto-N-fucopentaose 1(LNFPl)

BCL11A基因变异所致Dias-Logan综合征1例患儿的遗传学分析

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中华医学遗传学杂志 2024年第9期41卷 1096-1099页

作者：李林飞罗淑颖张耀东尚清张万存刘磊张小慢梅世月郑州大学附属儿童医院河南省儿童医院郑州儿童医院河南省儿童遗传代谢性疾病重点实验室郑州450018

目的对1例Dias-Logan综合征患儿的临床特征及基因变异特点进行分析。方法选取2022年7月于郑州大学附属儿童医院康复医学科门诊就诊的1例言语障碍、自幼精神运动发育迟缓的患儿为研究对象。收集患儿的临床资料,采集患儿及其父母的外周静... 详细信息

目的对1例Dias-Logan综合征患儿的临床特征及基因变异特点进行分析。方法选取2022年7月于郑州大学附属儿童医院康复医学科门诊就诊的1例言语障碍、自幼精神运动发育迟缓的患儿为研究对象。收集患儿的临床资料,采集患儿及其父母的外周静脉血样并提取基因组DNA。通过全外显子组测序确定可疑致病变异后,应用Sanger测序在该家系中进行验证。本研究通过郑州大学附属儿童医院医学伦理委员会的审查(伦理号:2023-K-011)。结果患儿的主要临床表现为全面发育迟缓、小头畸形、特殊面容及行为异常,基因检测结果提示患儿BCL11A基因存在c.561_567delACACGCA(p.Q187fs*7)杂合移码变异,父母未携带该变异。根据美国医学遗传学与基因组学学会(ACMG)相关遗传变异分类标准与指南,该变异被判定为致病性(PVS1+PS2+PM2_Supporting)。结论BCL11A基因杂合变异可能是导致该患儿Dias-Logan综合征的遗传学病因,为临床决策和遗传咨询提供了依据。

关键词： Dias-Logan综合征 BCL11A基因新发变异