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miR-26b-5p靶向VEGF抑制非小细胞肺癌A549细胞增殖、迁移和侵袭的机制研究

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临床肺科杂志 2024年第3期29卷 375-381页

作者：柳鑫姜晓丽马雪梅佳木斯大学附属第一医院呼吸内科黑龙江佳木斯154000

目的探讨miR-26b-5p对非小细胞肺癌细胞增殖、迁移和侵袭的影响及其作用机制。方法培养人正常肺上皮细胞BEAS-2B和非小细胞肺癌细胞A549,通过qPCR检测BEAS-2B和A549细胞中miR-26b-5p和VEGF mRNA的表达水平。通过Western Blot检测BEAS-2B... 详细信息

目的探讨miR-26b-5p对非小细胞肺癌细胞增殖、迁移和侵袭的影响及其作用机制。方法培养人正常肺上皮细胞BEAS-2B和非小细胞肺癌细胞A549,通过qPCR检测BEAS-2B和A549细胞中miR-26b-5p和VEGF mRNA的表达水平。通过Western Blot检测BEAS-2B和A549细胞中E-cadherin、Vimentin和VEGF蛋白的表达水平。生物信息学预测VEGF与miR-26b-5p的靶向关系。使用脂质体法将NC-mimic和miR-26b-5p-mimic转染A549细胞,通过CCK8法、EDU实验检测miR-26b-5p对细胞生长的影响;通过划痕实验和Transwell侵袭实验检测miR-26b-5p对细胞迁移和侵袭的影响;通过Western Blot检测对E-cadherin、Vimentin和VEGF蛋白表达的影响。结果与BEAS-2B细胞相比,在A549细胞中,miR-26b-5p表达水平显著下降(P<0.05),VEGF的mRNA和蛋白表达水平显著上升(P<0.05),E-cadherin蛋白的表达水平显著下降(P<0.05),而Vimentin的蛋白表达水平显著上升(P<0.05)。与NC-mimic组相比,miR-26b-5p-mimic组在24h、48h以及72h时细胞吸光值显著减少(P<0.05),24h划痕距离显著增加(P<0.05),24h侵袭细胞数显著减少(P<0.05),E-cadherin蛋白表达显著上调(P<0.05)、Vimentin和VEGF蛋白表达显著下调(均P<0.05)。结论过表达miR-26b-5p可以通过靶向抑制VEGF的表达,从而抑制非小细胞肺癌A549细胞的增殖、迁移和侵袭。

关键词： miR-26b-5p 血管内皮生长因子非小细胞肺癌细胞增殖

参麦注射液治疗气阴两虚型冠心病心绞痛患者的效果观察

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中外医药研究 2024年第15期3卷 99-101页

作者：赖小桃蒋小莉焦爱军广西医科大学药学院广西壮族自治区南宁530021 广西康晟制药有限责任公司广西壮族自治区南宁530225

目的:探讨参麦注射液治疗气阴两虚型冠心病的效果。方法:选取2020年1月—2023年10月贺州市人民医院收治的气阴两虚型冠心病患者120例作为研究对象,使用随机数字法分为观察组与对照组,各60例。对照组采用常规西药治疗,观察组在对照组基... 详细信息

目的:探讨参麦注射液治疗气阴两虚型冠心病的效果。方法:选取2020年1月—2023年10月贺州市人民医院收治的气阴两虚型冠心病患者120例作为研究对象,使用随机数字法分为观察组与对照组,各60例。对照组采用常规西药治疗,观察组在对照组基础上采用参麦注射液治疗。比较两组治疗效果、6min步行距离、生活质量、不良反应发生率。结果:观察组治疗总有效率高于对照组,差异有统计学意义(P=0.013)。治疗前,两组6min步行距离比较,差异无统计学意义(P>0.05);治疗后,两组6min步行距离长于治疗前,观察组长于对照组,差异有统计学意义(P0.05);治疗后,两组生理功能、社会功能、情感职能与精神健康评分高于治疗前,观察组高于对照组,差异有统计学意义(P<0.05)。观察组不良反应发生率低于对照组,差异有统计学意义(P=0.048)。结论:参麦注射液联合常规治疗在气阴两虚型冠心病患者中的效果显著,可改善患者心功能,提高生活质量,减少不良反应。

关键词：参麦注射液气阴两虚型冠心病心功能

Mineral provinces and matter provenance of the surficial sediments in the western Philippine Sea:implication for modern sedimentation in West Pacific marginal basin

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Acta Oceanologica Sinica 2007年第3期26卷 44-55页

作者： REN Xiangwen YAN Quanshu SHI Xuefa WANG Kunshan jiang xiaoli Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China Key Laboratory of Marine Sedimentology and Environmental Geology First Institute of Oceanography State Oceanic Adminis-tration Qingdao 266061 China Graduate School Chinese Academy of Sciences Beijing 100049 China

The characteristics and distribution patterns of detrital minerals （0. 063 -0. 125 mm） in marine sediments provide a significant indicator for the identification of the origin of sediment. The detrital mineral composition of 219 surface sediment samples was ana- lysed to identify the distribution of sediments within the western Philippine Sea. The area can be divided into three mineral provinces ：（Ⅰ） province east of the Philippine Trench, the detrital minerals in this province are mainly composed of calcareous or siliceous organisms, with the addition of volcanogenic minerals from an adjacent island arc; （Ⅱ） middle mineral province, clastic minerals including feldspar, quartz and colorless volcanic glass, sourced from seamounts with intermediate-acid volcanic rock, or erupting intermediate-acid volcano; （Ⅲ） province west of the Palau--Kyushu Ridge, the matter provenance within this province is complex; the small quantity of feldspar and quartz may be sourced from seamounts or erupting volcano with intermediate - acid composition, with a component of volcanic scoria sourced from a volcano erupting on the Palau--Kyushu Ridge. it is suggested that, （ 1 ） Biogenic debris of the study area is closely related to water depth, with the amount of biogenic debris controlled by carbonate lysocline. （2） Volcaniclastic matter derived from the adjacent island arc can be entrained by oceanic currents and transported towards the abyssal basin over a short distance. The weathering products of volcanic rocks of the submarine plateau （e. g. , Benham Plateau） and adjacent ridges provide an important source of detrital sedimentation, and the influence scope of them is constrained by the intensity of submarine weathering. （3） Terrigenous sediments from the continent of Asia and the adjacent Philippine island arc have little influence on the sedimentation of this study area, and the felsic mineral component is probably sourced from volcanic seamounts of intermediate-acid composition.

关键词： light mineral mineral province matter provenance western Philippine Sea

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标准化病人在儿童呼吸科教学中的应用价值

继续医学教育 2024年第2期38卷 93-96页

作者：姜小丽邓昱李渠北叶泽慧重庆医科大学附属儿童医院呼吸科重庆400014 国家级住院医师规范化培训示范基地重庆400014

目的探讨标准化病人在儿童呼吸科教学中的应用价值。方法选取2021年1月至2022年6月在重庆医科大学附属儿童医院呼吸科二病房参加住院医师规范化培训的住院医师,共计60名,分为对照组与观察组,每组30名。对照组采用传统教学方法,观察组采... 详细信息

目的探讨标准化病人在儿童呼吸科教学中的应用价值。方法选取2021年1月至2022年6月在重庆医科大学附属儿童医院呼吸科二病房参加住院医师规范化培训的住院医师,共计60名,分为对照组与观察组,每组30名。对照组采用传统教学方法,观察组采用标准化病人(standardized patients,SP)教学模式。示范教学内容为“儿童呼吸常见疾病的病史采集、肺部查体及病例分析”。教学结束后,比较2组住院医师的理论考试及实践操作考试成绩,通过调查问卷的形式调查住院医师对SP教学模式的满意度。结果对照组与观察组住院医师理论考试的成绩基本相近,2组比较差异无统计学意义(P>0.05)。观察组住院医师实践操作考试成绩(92.80±1.24)分高于对照组(85.40±3.69)分,差异有统计学意义(P<0.001)。住院医师满意度结果显示:观察组中大多数住院医师认为SP的应用可以提高病史采集逻辑性、提高体格检查能力、提高临床思维能力、提高诊治能力、提高医患沟通能力、提高学习兴趣。结论SP教学模式的应用可以提高住院医师病史采集、体格检查及临床思维能力,提高住院医师的诊治能力及医患沟通能力,在儿童呼吸科临床教学中引入SP教学模式可以提高教学效果,住院医师接受度高,具有明确的必要性及应用价值,可为呼吸科临床教学提供新的手段与思路,提高了儿科医学生的专业素质及人文素质,为国家培养更多优秀的儿科医生添砖加瓦。

关键词：标准化病人规范化培训呼吸科儿童教学模式住院医师

Preparation of CuCr alloys by thermit-reduction electromagnetic stirring

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Journal of University of Science and Technology Beijing 2007年第6期14卷 538-542页

作者： Zhihe Dou Tingan Zhang Haien Yu Liping Niu xiaoli jiang Huan Yang School of Materials and Metallurgy Northeastern University Shenyang 110004 China

The influences of the additive CaF2, different molds, mold pre-heating temperature, electromagnetic stirring, and alloying elements on CuCr have been investigated respectively during the preparation of CuCr alloys by thermit-reduction electromagnetic stirring. The results indicate that the reasonable reactant mass ratio of CuO： Cr2O3：Al is 100：140：160; CaF2 can reduce the solidification point of slags to improve the metal separating efficiency from slags; the crystal particles become thinner because of the high cooling velocity in the metal mold; while casting in the graphite mold, the casting properties of CuCr improve with the increase of pre-heating temperature; the compact alloys are prepared at 500℃; electromagnetic stirring can prevent the growth of dendrite crystal into refine crystal particles, as well as homogenize Cu and Cr to improve the CuCr properties; the optimal stirring time is 7 min; when the alloying elements Ni and Co are added to the reactants, elements Cu and Cr can distribute evenly but the crystal particles become thick.

关键词： contact material CuCr alloy thermit reduction electromagnetic stirring additive

Magnetic properties and structure of (001)-oriented [CoPt/C]_n /Ag nanocomposite films on the glass substrates

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Rare Metals 2006年第3期25卷 265-269页

作者： JIN Tao XU Xiaohong WANG Fang LI xiaoli jiang Fengxian YANG Zhiguang School of Chemistry and Materials Science Shanxi Normal University Linfen 041004 China

The highly （1301） oriented triple system of [CoPt/C]n/Ag films was deposited on glass substrates by DC and RF magnetron sputtering. After annealing at 600℃ for 30 min, thin films become magnetically hard with coercivities in the range of 160-875 kA/m because of high anisotropy associated with the L10 ordered phase. C doping plays an important role in improving （001） texture and reducing the intergrain interactions. The oriented growth of CoPt films was influenced strongly by the number of repetitions （n） of CoPt/C. By controlling the C content and the number of repetitions （n） of CoPt/C, nearly perfect （001） orientation can be obtained in the [CoPt3nm/C3nm]5/Ag50 nm.

关键词： [CoPt/C]n/Ag multilayer films (001) orientation C content number of repetition

Self-assembled structure of cyanine dyes: J-aggregates characteristics on optical information storing microcrystals

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Rare Metals 2006年第Z1期25卷 158-162页

作者： jiang xiaoli LI Xiaowei LAI Weidong MENG Tao FENG Xiaomin ZHANG Jixian College of Physics Science & Technology Hebei University Baoding 071002 China College of Physics Science & Technology Hebei University Baoding 071002 China College of Physics Science & Technology Hebei University Baoding 071002 China College of Physics Science & Technology Hebei University Baoding 071002 China College of Physics Science & Technology Hebei University Baoding 071002 China College of Physics Science & Technology Hebei University Baoding 071002 China

Dye sensitization is a fundamental function for solar cell and silver halide (AgX) microcrystal to increase the optoelectronic conversion efficiency. In this paper, the spectral properties and self-assembled structure of three types of cyanine dyes, adsorbed both on (100) surface of 0.4 μm AgBr cubic crystal and (111) surface of 1.8 μm AgBr tabular crystal, were studied with combination of spectroscopy and atomic force microscopy (AFM) technique. Rectangular aggregation structure is formed on the crystal faces of (100) and (111) for both anionic and cationic dyes, while herringbone-stacking structure is formed by anionic-cationic dye, and a J-band spectrum is correspondingly detected. The photoelectron property of dye-sensitized samples was also investigated with microwave absorption and dielectric spectrum detection technology. After excited by a 355 nm fast-pulse laser, the photoelectron decay process of anionic-cationic dye sensitized sample is the fastest. This indicates that the affection of anionic-cationic dye aggregates to the photoelectron decay is the biggest, and the sensitization is more efficient.

关键词： dye sensitization aggregate optoelectronic conversion photoelectron

Synergistic Effect of Dual-Doped Carbon on MO_(2)C Nanocrystals Facilitates Alkaline Hydrogen Evolution

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Nano-Micro Letters 2023年第10期15卷 101-111页

作者： Min Zhou xiaoli jiang Weijie Kong Hangfei Li Fei Lu Xin Zhou Yagang Zhang School of Materials and Energy University of Electronic Science and Technology of ChinaChengdu 611731People’s Republic of China State Key Laboratory of Electronic Thin Films and Integrated Devices University of Electronic Science and Technology of ChinaChengdu 610054People’s Republic of China College of Physical Science and Technology Yangzhou UniversityYangzhou 225002People’s Republic of China Department of Chemical Engineering Polytechnique MontrealMontrealQC H3C 3A7Canada

Molybdenum carbide(MO_(2)C)materials are promising electrocatalysts with potential applications in hydrogen evolution reaction(HER)due to low cost and Pt-like electronic ***,their HER activity is usually hindered by the strong hydrogen binding ***,the lack of water-cleaving site's makes it difficult for the catalysts to work in alkaline ***,we designed and synthesized a B and N dual-doped carbon layer that encapsulated on MO_(2)C nanocrystals(MO_(2)C@BNC)for accelerating HER under alkaline *** electronic interactions between the MO_(2)C nanocrystals and the multiple-doped carbon layer endow a near-zero H adsorption Gibbs free energy on the defective C atoms over the carbon ***,the introduced B atoms afford optimal H_2O adsorption sites for the water-cleaving ***,the dual-doped MO_(2)C catalyst with synergistic effect of non-metal sites delivers superior HER performances of a low overpotential(99 mV@10 mA cm^(-2))and a small Tafel slope(58.1 mV dec^(-1))in 1 M KOH ***,it presents a remarkable activity that outperforming the commercial 10%Pt/C catalyst at large current density,demonstrating its applicability in industrial water *** study provides a reasonable design strategy towards noble-metal-free HER catalysts with high activity.

关键词： Molybdenum carbide Hydrogen evolution reaction Dual-doped Synergistic effect Superior performances

Selection Methodology of High-Cycle Fatigue Analysis Approach for Damage Estimation in Welded Structural Joints

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Journal of Marine Science and Application 2022年第4期21卷 116-133页

作者： Skirmantas Pargalgauskas xiaoli jiang Marcel Edelkamp Dingena Schott Department of Maritime and Transport Technology Delft University of TechnologyDelft 2628CDNetherlands In Summa Inovation B.V. Raamsdonksveer 4941VVNetherlands

The main purpose of this paper is to provide a summarized general guideline to aid decision making of choosing the type of fatigue analysis approach,best suited for modelling and evaluating high-cycle fatigue damage in welded structural *** describes how addition of stress concentration and stress direction information into fatigue assessment methodology affect simulated fatigue damage accumulation results and when it is beneficial or necessary to use a particular fatigue damage estimation *** focus is on stress-life curve based approaches,particularly when deciding between variants of nominal,hot-spot or multiaxial fatigue assessment approaches for evaluating fatigue damage within welded joint *** is illustrated through application of proposed methodology to choose and perform fatigue assessment for a non-conventional load-bearing tubular joint structure within a floating lemniscate crane upper arm,which has been observed of being prone to aggressive crack propagation within its *** within the structure is estimated using two non-optimal fatigue analysis approaches to verify applicability of proposed selection *** are then summarized through comparative assessment and findings are discussed based on what leads to result changes within each fatigue damage analysis approach.

关键词： Fatigue analysis Multiaxial fatigue Hot-spot fatigue Methodology Welded joints