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Parentage Analysis of Dongfang No.2, a Hybrid of a Female Gametophyte Clone of Laminaria japonica (Laminariales, Phaeophyta) and a Male Clone of L. longissima

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Journal of Ocean University of China 2008年第2期7卷 193-198页

作者： YANG Guanpin liAO Meijie li xiaojie CONG Yizhou QU Shancun WANG Tongyong College of Marine life Sciences Ocean University of China Qingdao 266003 P. R. China Shandong Oriental Ocean Sci-Tec Co. Ltd. Yantai 264003 P R. China Wendeng Fishery Technique Popularization Station of Shandong Province Wendeng 264400 P R. China

The cultivation of the first filial generation of monoploid gametophyte clones of different Laminaria species (hybrid Laminaria) is an effective way of utilizing heterozygous vigor (heterosis). A female gametophyte clone of L. japonica and a male gametophyte clone of L. longissima were hybridized, generating Dongfang No.2 hybrid Laminaria. The parentage of this hybrid Laminaria was determined using AFLP of total DNA, SNP of the ITS region of ribosomal RNA transcription unit and microsatellite DNA variation at two loci. In addition to 167 AFLP bands shared by Dongfang No.2, L. japonica and L. longissima, Dongfang No.2 hybrid Laminaria shared another 70 and 55 bands with L. japonica and L. longissima, respectively, which were obviously more than 11 bands shared by L. japonica and L. longissima. Dongfang No.2 held both ‘T’ and ‘C’ at position 847 of the ITS region, while ‘T’ at this position was specific for L. japonica and ‘C’ for L. longissima, respectively. Dongfang No.2 also held the microsatellite DNA alleles of two parents together at two microsatellite DNA marker loci. These observations clearly proved that Dongfang No.2 is a true hybrid of L. japonica and L. longissiuma. Unfortunately, the origin of the chloroplast of Dongfang No.2 was not determined based on the variation of RuBisCo spacer. More sequence variants of both ITS region and RuBisCo spacer were identified in Dongfang No.2 and most of them did not exist in either L. japonica or L. longissima. The unexpected variants may be due to the mutation of ga- metophyte clones occurring during their vegetative amplification.

关键词： hybrid Laminaria gametophyte clone heterosis Laminaria japonica Laminaria longissima

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面向自然语言需求的验证性质生成方法

小型微型计算机系统 2024年第1期45卷 84-92页

作者：李晓劼杨志斌王翰丰周勇李维南京航空航天大学计算机科学与技术学院南京211106 高安全系统的软件开发与验证技术工信部重点实验室南京211106 航宇救生装备有限公司襄阳441003

安全关键系统和软件的安全性、可靠性需要形式化验证来保障,使用形式化验证的前提是从自然语言需求文本中提取相关验证性质并将其转化为形式化规约,这已成为当前形式化验证领域研究的热点和难点.当前的形式化规约提取工作大多针对英文需... 详细信息

安全关键系统和软件的安全性、可靠性需要形式化验证来保障,使用形式化验证的前提是从自然语言需求文本中提取相关验证性质并将其转化为形式化规约,这已成为当前形式化验证领域研究的热点和难点.当前的形式化规约提取工作大多针对英文需求,较少针对中文自然语言需求.此外,由于AADL具有强大的表达能力和完善的验证机制,已成为航空航天领域的主要建模语言之一,而现有的工作较少考虑如何从需求中提取AADL模型的验证性质.为了解决上述问题,本文提出一种面向自然语言需求的AADL模型验证性质自动生成方法,从自然语言需求中提取验证的相关性质,并将其转化为AADL模型验证工具AGREE可识别的形式化规约.首先,定义了模式定义语言(Contract Pattern Language,CPL),将需求划分为不同模式,并给出由固定句型和占位符组成的需求模板;其次,通过自然语言处理技术解析需求文本,获取替换需求模板中占位符的原子命题,以便生成完整的形式化规约;最后,设计并实现了相关工具,并将其用于工业界实际案例来说明该方法的可用性和有效性.

关键词：形式化验证模式定义语言自然语言处理规约生成

心理弹性在老年胃癌术后患者认知功能和睡眠障碍间的中介效应

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河北医药 2024年第2期46卷 297-299页

作者：王敏李来有李晓杰王静茹李晓娜吕景霞许夕霞李勇河北医科大学第四医院外三科石家庄市050011 河北医科大学第四医院神经内科石家庄市050011

目的探讨心理弹性在老年胃癌术后患者认知功能和睡眠障碍之间的中介效应。方法采用便利抽样的方法,随机抽取河北医科大学第四医院外三科246例老年胃癌术后患者。使用一般资料调查量表、匹兹堡睡眠质量指数量表(Pittsburgh Sleep Quality... 详细信息

目的探讨心理弹性在老年胃癌术后患者认知功能和睡眠障碍之间的中介效应。方法采用便利抽样的方法,随机抽取河北医科大学第四医院外三科246例老年胃癌术后患者。使用一般资料调查量表、匹兹堡睡眠质量指数量表(Pittsburgh Sleep Quality Index,PSQI)、心理弹性量表(Connor-Davidson resilience scale,CD-RISC)和简易智能精神状态量表(Mini-mental State Examination,MMSE)分别调查老年胃癌术后患者的睡眠质量、心理韧性和认知功能。结果PSQI、CD-RISC和简MMSE得分分别为(5.63±1.41)、(60.31±7.85)和(17.56±2.97)分,PSQI总分与MMSE评分、CD-RISC总分呈负相关(R值分别为-0.421、-0.542,P<0.05),MMSE总分与CD-RISC总分呈正相关(R=0.874,P<0.05),线性回归分析结果显示心理弹性在认知功能和睡眠障碍之间起部分中介作用,贡献率为71.28%。结论认知功能通过心理弹性间接影响老年胃癌术后患者的睡眠质量,老年胃癌术后患者的睡眠质量和认知功能有待进一步提高。

关键词：老年患者胃癌术后心理弹性睡眠障碍认知功能

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Multi-Task Learning Using Attention-Based Convolutional Encoder-Decoder for Dilated Cardiomyopathy CMR Segmentation and Classification

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Computers, Materials & Continua 2020年第5期63卷 995-1012页

作者： Chao Luo Canghong Shi xiaojie li Xin Wang Yucheng Chen Dongrui Gao Youbing Yin Qi Song Xi Wu Jiliu Zhou 无 College of Computer Science Chengdu University of Information TechnologyChengdu610000China College of Information Science and Technology Southwest Jiaotong UniversityChengdu610000China West China Hospital Sichuan UniversityChengdu610000China AI Institute CuraCloud CorporationSeattle98101USA

Myocardial segmentation and classification play a major role in the diagnosis of cardiovascular *** Cardiomyopathy(DCM)is a kind of common chronic and life-threatening *** diagnostics significantly increases the chances of correct treatment and ***,accurate and rapid diagnosis of DCM is still challenge due to high variability of cardiac structure,low contrast cardiac magnetic resonance(CMR)images,and intrinsic noise in synthetic CMR images caused by motion artifact and cardiac ***,visual assessment and empirical evaluation are widely used in routine clinical diagnosis,but they are subject to high inter-observer variability and are both subjective and *** solve this problem,we proposed an effective unified multi-task framework for dilated cardiomyopathy CMR segmentation and classification simultaneously,and we firstly update one independent encoder from both recovery decoder and parallel attention path sharing some partial *** can encode both task choices into good embedding,but each one can achieve significant improvements respectively from the given *** consists of three branches:extraction path,attention path,and recovery path,which allows the model to learn more higher-level intermediate representations and makes a more accurate *** validated our approach on a DCM dataset,which contains 1155 CMR LGE *** results show that our multi-task network has achieved accuracy of 97.63%,AUC of 98.32%,demonstrating effectively segmenting the myocardium,quickly and accurately diagnosing the presence or absence of dilation.

关键词： Dilated cardiomyopathy multitasking network attention classification segmentation

A self-adaptive negative selection algorithm used for anomaly detection

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Progress in Natural Science:Materials International 2009年第2期19卷 261-266页

作者： Jinquan Zeng,xiaojie liu,Tao li,Caiming liu,lingxi Peng,Feixian Sun Department of Computer Science,Sichuan University,Chengdu 610065,China Department of Computer Science Sichuan University Chengdu 610065 China

A novel negative selection algorithm (NSA),which is referred to as ANSA,is presented. In many actual anomaly detection systems,the training data are just partially composed of the normal elements,and the self/nonself space often varies over time. Therefore,anom-aly detection system has to build the profile of the system based on a part of self elements and adjust itself to adapt those variables. However,previous NSAs need a large number of self elements to build the profile of the system,and lack adaptability. In order to over-come these limitations,the proposed approach uses a novel technique to adjust the self radius and evolve the nonself-covering detectors to build an appropriate profile of the system. To determine the performance of the approach,the experiments with the well-known data-set were performed. Results exhibited that our proposed approach outperforms the previous techniques.

关键词： Artificial immune system Anomaly detection Negative selection algorithm

外科治疗克罗恩病合并肠穿孔25例的临床特征解析

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腹部外科 2024年第4期37卷 276-280,295页

作者：李小洁马田田姚潇靳安东陶凯雄高金波华中科技大学同济医学院附属协和医院胃肠外科湖北武汉430022 陕西省人民医院普外二科陕西西安710068

目的总结分析克罗恩病(Crohn′s disease,CD)合并肠穿孔的临床特征以及探讨外科治疗的具体疗效。方法纳入从2000年1月至2022年10月于华中科技大学同济医学院附属协和医院就诊的CD合并肠穿孔病人25例,对其进行回顾性分析,总结回顾病人的... 详细信息

目的总结分析克罗恩病(Crohn′s disease,CD)合并肠穿孔的临床特征以及探讨外科治疗的具体疗效。方法纳入从2000年1月至2022年10月于华中科技大学同济医学院附属协和医院就诊的CD合并肠穿孔病人25例,对其进行回顾性分析,总结回顾病人的临床特征、手术治疗情况以及临床随访资料。结果25例合并肠穿孔的CD病人以男性多见(84%),确诊年龄多处于17~40岁之间(80%),穿孔部位以回肠多见(72%),病变性质主要为穿透性病变(80%)。所有病人均采取手术治疗,手术方案以腹腔镜辅助下肠切除为主(52%),多行肠造口(72%);术后并发症发生率为28%,其中主要以感染性疾病为主。术前术后病人均易发生贫血以及低蛋白血症。至随访截止日期为止,共12例复发(48%),1例死亡。结论CD合并肠穿孔以男性多见,穿孔多发生在回肠,入院后均行急诊手术。及时明确病因,注重围手术期管理,合理选择手术方式,可以减少CD合并肠穿孔病人术后并发症发生率及复发率。

关键词：克罗恩病肠穿孔临床特征手术预后

Morphological, Mechanical and Thermal Properties of Poly(lactic acid)(PLA)/Cellulose Nanofibrils(CNF) Composites Nanofiber for Tissue Engineering

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Journal of Wuhan University of Technology(Materials Science) 2019年第1期34卷 207-215页

作者： YANG Zhangqiang SI Junhui School of Materials Science and Engineering Fuzhou University School of Materials Science and Engineering Fujian University of Technology Fujian Provincial Key Laboratory of Advanced Materials Processing and Application

Composite nanofiber membranes based on biodegradable poly(lactic acid)(PLA) and cellulose nanofibrils(CNF) were produced via electrospinning. The influence of CNF content on the morphology, thermal properties, and mechanical properties of PLA/CNF composite nanofiber membranes were characterized by field scanning electron microscopy(FE-SEM), differential scanning calorimetry(DSC), thermogravimetric analysis(TGA), and dynamic mechanical analysis(DMA), respectively. The results show that the PLA/CNF composite nanofibers with smooth, free-bead surface can be successfully fabricated with various CNF contents. The introduction of CNF is an effective approach to improve the crystalline ability, thermal stability and mechanical properties for PLA/CNF composite fibers. The Young's moduli and tensile strength of the PLA/CNF composite nanofiber reach 106.6 MPa and 2.7 MPa when the CNF content is 3%, respectively, which are one times higher and 1.5 times than those of pure PLA nanofiber. Additionally, the water contact angle of PLA/CNF composite nanofiber membranes decreases with the increase of the CNF loading, resulting in the enhancement of their hydrophilicity.

关键词： poly(lactic acid)(PLA) cellulose nanofibrils(CNF) electrospinning mechanical and thermal properties

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军人前交叉韧带损伤的危险因素和治疗现状

空军航空医学 2024年第1期41卷 59-65页

作者：谢泊文厉晓杰杨丰源张红星陈志强李天琪杜俊杰空军军医大学空军特色医学中心骨科北京100142 安徽医科大学第五临床学院空军临床学院合肥230032

军事训练的难度和强度不断提高,及体育活动的规范性不足等因素,导致前交叉韧带(anterior cruciate ligament,ACL)损伤在军人群体中变得更加常见。这种损伤不仅会限制膝关节的功能,甚至可能提前终止患者的军事生涯,给军队的战备力量造成... 详细信息

军事训练的难度和强度不断提高,及体育活动的规范性不足等因素,导致前交叉韧带(anterior cruciate ligament,ACL)损伤在军人群体中变得更加常见。这种损伤不仅会限制膝关节的功能,甚至可能提前终止患者的军事生涯,给军队的战备力量造成损失。笔者通过在中国知网、维普、Web of Science、PubMed等数据库查阅77篇相关文献,对ACL损伤的流行病学、危险因素、诊断、治疗和康复训练等方面进行综述,旨在为临床诊疗工作和制定预防措施提供参考。

关键词：前交叉韧带损伤重建方式流行病学综述军人

Effects of Different Culture Conditions and Growth States on the Structure and Hemostatic Properties of Coscinodiscus sp.

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Journal of Ocean University of China 2024年第4期23卷 1121-1134页

作者： HE Zheng li Qinfeng liU Jinfeng HUI Guangyan FENG Chao CHENG xiaojie CHEN Xiguang College of Marine life Science Sanya Oceanographic InstitutionOcean University of ChinaQingdao 266003China Laboratory for Marine Drugs and Bioproducts Qingdao Marine Science and Technology CenterQingdao 266237China Department of Stomatology Qingdao Women and Children’s HospitalQingdao 266034China Department of Stomatology Qingdao Special Servicemen Recuperation Center of PLA NavyQingdao 266071China

As a renewable marine inorganic material,Coscinodiscus *** significant potential in the field of rapid ***,the low yield of Coscinodiscus *** limits the *** this study,two new culture modes were adopted to increase the production of Coscinodiscus sp.,the effect of changes in culture conditions and growth status on the hemostatic activity of diatoms was *** prevent Coscinodiscus *** sinking in culture,the suspension culture mode was realized by adding0.5%*** semi-continuous high nutrient concentration culture mode increased the cell density of Coscinodiscus *** 11000cells mL^(-1)and shorten the culture cycle to 5 *** terms of coagulation activity,the addition of frustules reduced the in vitro coagulation time by half and the activation time of coagulation by 70%.The hemolysis rate and cytotoxicity of frustules harvested in the two culture modes did not change *** results showed that suspension culture mode and high nutrient concentration culture mode only changed the growth state of Coscinodiscus sp.,while the hemostatic performance remained stable.

关键词： diatom culture Coscinodiscus sp frustule hemostatic material hemorrhage control