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Decreased LDHB expression in breast tumor cells causes NK cell activation and promotes tumor progression

Cancer Biology & Medicine 2024年第6期21卷 513-540页

作者： Zhihong Luo xiaohua huang Xinyi Xu Kefeng Wei Yi Zheng Ke Gong Wenhua Li Hubei Key Laboratory of Cell Homeostasis College of Life Sciences Wuhan University Wuhan University Shenzhen Research Institute Central Laboratory University of Chinese Academy of Sciences-Shenzhen Hospital Hubei Province Key Laboratory of Allergy and Immunology and Department of Immunology School of Basic Medical Sciences Wuhan University

Objective: Abnormal metabolism is the underlying reason for breast cancer progression. Decreased lactate dehydrogenase B(LDHB) has been detected in breast cancer but the function of LDHB remains unknown.Methods: Western blot was used to analyze LDHB expression in breast cancer cells. The impact of LDHB on tumor cell migration and invasion was determined using Transwell assays, wound healing assays, and a mouse lung metastasis model. Subcutaneous tumor formation, a natural killer(NK) cell cytotoxicity assay, and flow cytometry evaluated NK cell activation. Immunofluorescence and quantitative real-time PCR detected NK cell activation markers. Kaplan-Meier analysis evaluated the effect of immune cell infiltration on prognosis. Single-sample gene set enrichment analysis determined NK cell activation scores. A support vector machine predicted the role of LDHB in NK cell activation.Results: In this study we showed that LDHB inhibits the breast cancer cell metastasis and orchestrates metabolic reprogramming within tumor cells. Our results revealed that LDHB-mediated lactic acid clearance in breast cancer cells triggers NK cell activation within the tumor microenvironment. Our findings, which were confirmed in a murine model, demonstrated that LDHB in tumor cells promotes NK cell activation and ultimately results in the eradication of malignant cells. Clinically, our study further validated that LDHB affects immune cell infiltration and function. Specifically, its expression has been linked to enhanced NK cell-mediated cytotoxicity and improved patient survival. Furthermore, we identified LDHB expression in tumors as an important predictor of NK cell activation, with strong predictive ability in some cancers.Conclusions: Our results suggest that LDHB is a promising target for activating the tumor immune microenvironment in breast cancer, where LDHB-associated lactic acid clearance leads to increased NK cell activity. This study highlights the critical role of LDHB in regulating immune responses and its potential as a the

关键词： Breast cancer NK cells lactate dehydrogenase B lactic acid tumor immunity

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Extracellular Disintegration of Viral Proteins as an Innovative Strategy for Developing Broad-Spectrum Antivirals Against Coronavirus

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CCS Chemistry 2024年第2期6卷 487-496页

作者： Ke Sun Zhe Ding Xiaoying Jia Haonan Cheng Yingwen Li Yan Wu Zhuoyu Li xiaohua huang Fangxu Pu Entao Li Guiyou Wang Wei Wang Yun Ding Gary Wong Sandra Chiu Jiaming Lan Aiguo Hu Shanghai Key Laboratory of Advanced Polymeric Materials School of Materials Science and EngineeringEast China University of Science and TechnologyShanghai 200237 CAS Key Laboratory of Molecular Virology&Immunology Chinese Academy of SciencesShanghai 200031 University of the Chinese Academy of Sciences Beijing 100049 State Key Laboratory of Virology Center for Biosafety Mega-ScienceWuhan Institute of VirologyChinese Academy of SciencesWuhan 430071 Division of Life Sciences and Medicine University of Science and Technology of ChinaHefeiAnhui 230027 Department of Clinical Laboratory The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui 230001

The coronavirus disease 2019(COVID-19)pandemic,caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),has claimedmillions of lives and caused innumerable economic losses worldwide.Unfortunately,state-of-the-art treatments still lag behind the continual emergence of new variants.Key to resolving this issue is developing antivirals to deactivate coronaviruses regardless of their structural evolution.Here,we report an innovative antiviral strategy involving extracellular disintegration of viral proteins with hyperanion-grafted enediyne(EDY)molecules.The core EDY generates reactive radical species and causes significant damage to the spike protein of coronavirus,while the hyperanion groups ensure negligible cytotoxicity of the molecules.The EDYs exhibit antiviral activity down to nanomolar concentrations,and the selectivity index of up to 20,000 against four kinds of human coronavirus,including the SARS-CoV-2 Omicron variant,suggesting the high potential of this new strategy in combating the COVID-19 pandemic and a future“disease X.”

关键词： enediyne broad spectrum antiviral protein disintegration coronavirus polyanions

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Enrichment process of lanthanum as a nonessential trace element in leaf cells of lettuce(Lactuca sativa L.)

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Journal of Rare Earths 2022年第12期40卷 1969-1976,I0006页

作者： Mengzhu Cheng Xin Wang Yue Ben Shuya Zhang Lihong Wang Qing Zhou xiaohua huang National and Local Joint Engineering Research Center of Biomedical Functional Materials Jiangsu Collaborative Innovation Center of Biomedical Functional MaterialsSchool of Chemistry and Materials ScienceNanjing Normal UniversityNanjing 210023China Center for Plant Cell Biology Institute of Integrative Genome Biologyand Department of Botany and Plant SciencesUniversity of CaliforniaRiversideCA92521USA State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China

Rare earth elements(REEs)as nonessential trace elements are enriched in living organisms and threaten their health.To early detect and reduce REE enrichment in living organisms,scientists are focused on clarifying the enrichment process of REEs in living organisms and its risks.However,the enrichment process of REEs in edible plant cells has remained unclear.Herein,by using interdisciplinary methods and techniques,the enrichment process of lanthanum(La(Ⅲ))in the leaf cells of lettuce(Lactuca sativa L.)was investigated.(1)When La(Ⅲ)exposure dose is 0.5-5μmol/L,La(Ⅲ)is enriched outside the plasma membrane(PM).In this zone,La(Ⅲ)is bound to vitronectin-like protein(VN)to form La-VN complexes;(2)When La(Ⅲ)exposure dose is 5-20μmol/L,besides the zone outside the PM,La(Ⅲ)is also enriched on the PM and bound to arabinogalactan proteins(AGPs)to form La-AGPs complexes;(3)When La(Ⅲ)exposure dose is 20-140μmol/L,besides the zone outside and on the PM,La(Ⅲ)is enriched inside the PM;(4)When La(Ⅲ)exposure dose is 60-140μmol/L,malondialdehyde content(an important indicator of invisible damage)significantly increases.Thus,as La(Ⅲ)exposure dose increases,La(Ⅲ)gradually migrates from outside the PM to the PM and inside the PM,enriching in these zones in turn.The enriched La(Ⅲ)will cause invisible damage to lettuce leaf cells and even enter human bodies along food chains.These results provide references for investigating the enrichment process of REEs in plants and its environmental risks,and finding strategies to early detect and reduce REE enrichment in plants.

关键词： Nonessential trace element Lanthanum exposure Lettuce leaf cell Enrichment process Vitronectin-like protein Arabinogalactan proteins

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Identification of Epsin1 as a regulator for hepatic lipid and glucose metabolism

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Genes & Diseases 2023年第1期10卷 72-75页

作者： xiaohua huang Long Jin Zhengfeng Fang Lianqiang Che Yan Lin Shengyu Xu Yong Zhuo Mingzhou Li De Wu Bin Feng Animal Nutrition Institute Sichuan Agricultural UniversityChengduSichuan 611130China Key Laboratory of Animal Disease-Resistant Nutrition of Ministry of Education Sichuan Agricultural UniversityChengduSichuan 611130China College of Animal Science Sichuan Agricultural UniversityChengduSichuan 611130China

Obesity related non-alcoholic fatty liver disease(NAFLD),which can progress to steatohepatitis and insulin resistance,has become a major chronic liver disease worldwide.1 As an adaptor of clathrin-mediated endocytosis... 详细信息

关键词： metabolism healing wound

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Exploring NiCo2S4 nanosheets arrays by hydrothermal conversion for enhanced high-rate batteries

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Journal of Energy Chemistry 2019年第8期28卷 132-137,I0005页

作者： Jianbo Wu xiaohua huang Xinhui Xia Zhejiang Provincial Key Laboratory for Cutting Tools Taizhou UniversityTaizhou 318000 Zhejiang China Department of Materials Science and Engineering Zhejiang UniversityHangzhou 310027Zhejiang China Institute of Advanced Electrochemical Energy Xi'an University of TechnologyXi'an 710048 Shaanxi China

Highly cross-linked porous NiCo2S4 nanosheets arrays are synthesized by a high-efficiency hydrothermal conversion from the preformed electrodeposited NiCo2O4 arrays. By using thioacetamide as the sulfur source, the electrodeposited NiCo2O4 is directly converted into NiCo2O4 nanosheets arrays without hightemperature sulfurization. Higher porosity and better electrical conductivity are obtained for the NiCo2O4 nanosheets arrays. In addition, reduced diffusion paths of electrons/ions and alleviated volume expansion during cycling are achieved due to the unique porous structure of NiCo2S4. Consequently, as the cathode of alkaline batteries, the obtained NiCo2S4 nanosheets arrays show better electrochemical performance with a high specific capacity(83.5 m Ah g-1 at 0.5 A g-1) and better cycling stability(capacity retention of 93% after 5000 cycles) than the NiCo2O4 counterpart arrays(40.3 mAh g-1 at 0.5 A g-1). Our work demonstrates that sulfurization on binary metal oxides can greatly enhance electrochemical performance and shows a new way for construction of advanced electrodes for high-rate batteries.

关键词： Porous materials Thin films Alkaline batteries Metal oxides Energy storage and conversion

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Sharp bounds for spectral radius of nonnegative weakly irreducible tensors

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Frontiers of Mathematics in China 2019年第5期14卷 989-1015页

作者： Lihua YOU xiaohua huang Xiying YUAN School of Mathematical Sciences South China Normal UniversityGuangzhou 510631China Department of Mathematics Shanghai UniversityShanghai 200444China

We obtain the sharp upper and lower bounds for the spectral radius of a nonnegative weakly irreducible tensor.By using the technique of the representation associate matrix of a tensor and the associate directed graph of the matrix,the equality cases of the bounds are completely characterized by graph theory methods.Applying these bounds to a nonnegative irreducible matrix or a connected graph(digraph),we can improve the results of L.H.You,Y.J.Shu,and P.Z.Yuan[Linear Multilinear Algebra,2017,65(1):113-128],and obtain some new or known results.Applying these bounds to a uniform hypergraph,we obtain some new results and improve some known results of X.Y.Yuan,M.Zhang,and M.Lu[Linear Algebra Appl.,2015,484:540-549].Finally,we give a characterization of a strongly connected/c-uniform directed hypergraph,and obtain some new results by applying these bounds to a uniform directed hypergraph.

关键词： Nonnegative weakly irreducible tensors uniform(directed)hypergraph spectral radius bound

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Boosting fast lithium ion storage of Li4Ti5O12 by synergistic effect of vertical graphene and nitrogen doping

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Journal of Energy Chemistry 2020年第12期51卷 372-377页

作者： Yan Lin Jianbo Wu xiaohua huang Guoxiang Pan Xinhui Xia Zhejiang Provincial Key Laboratory for Cutting Tools Taizhou University Department of Materials Chemistry Huzhou University School of Materials Science and Engineering Zhejiang University

The advancement of power-type lithium ion batteries（LIBs） mainly depends on the construction of electrodes with superior electronic & ionic conductivity. In this work, a synergistic combination of nitrogen doped LiTiO（N-LTO） nanoparticles and highly conductive vertical graphene（VG） skeleton is proposed to obtain ultrafast Li ion storage performance via a facile hydrothermal-nitridation method. The as-synthesized composite nanosheets are composed of interconnected N-LTO shell and VG core to form the final N-LTO/VG core-shell arrays, which are systematically studied as the anodes of LIBs. In virtue of the positive synergistic effect of high conductive VG backbone and nitrogen doping, overwhelming advantages including high specific surface area, superb structural stability and enhanced conductivity are achieved in the well-designed N-LTO/VG electrodes. Accordingly, the as-synthesized N-LTO/VG electrode shows outstanding high rate capacity(142.5 m Ah gat 20 C) and superior long-term cycling stability（capacity retention of 98.9% after 10000 cycles at 5 C）, better than LTO/VG electrode without N doping.Our research provides a new strategy for the synthesis of high-performance hybrid electrodes for application in power-type LIBs.

关键词： Lithium titanate Anode Lithium ion batteries Vertical graphene High power

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Novel Non-Coplanar and Tertbutyl-Substituted Polyimides:Solubility,Optical,Thermal and Dielectric Properties

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中国化学:英文版 2015年第2期33卷 277-284页

作者： Chanjuan Liu Xianglin Pei xiaohua huang Chun Wei Xiaoyi Sun Key Laboratory of New Processing Technology for Nonferrous Metal&Materials Ministry of Educationand School of Material Science and EngineeringGuilin University of TechnologyGuilinGuangxi 541004China College of Chemistry and Chemical Engineering Central South UniversityChangshaHunan 410083China

A novel aromatic diamine monomer bearing tertbutyl and 4-tertbutylphenyl groups,3,3'-ditertbutyl-4,4'-diaminodiphenyl-4''-tertbutylphenylmethane(TADBP),was prepared and characterized.A series of non-coplanar polyimides(PIs)were synthesized via a conventional one-step polycondensation from TADBP and various aromatic dianhydrides including pyromellitic dianhydride(PMDA),3,3',4,4'-biphenyltetracarboxylic dianhydride(BPDA),4,4'-oxydiphthalic anhydride(OPDA),3,3',4,4'-benzophenone tetracarboxylic dianhydride(BTDA)and 4,4'-(hexafluoroisopropylidene)dipthalic anhydride(6FDA).All PIs exhibit excellent solubility in common organic solvents such as N,N-dimethylformamide(DMF),N,N-dimethylacetamide(DMAc),N-methyl-2-pyrrolidone(NMP),dimethyl sulfoxide(DMSO),chloroform(CHCl3),tetrahydrofuran(THF),and so on.Furthermore,the obtained transparent,strong and flexible polyimide films present good thermal stability and outstanding optical properties.Their glass transition temperatures(Tgs)are in the range of 298 to 347℃,and 10%weight loss temperatures are in excess of 490℃with more than 53%char yield at 800℃in nitrogen.All the polyimides can be cast into transparent and flexible films with tensile strength of 80.5-101 MPa,elongation at break of 8.4%-10.5%,and Young's modulus of 2.3-2.8 GPa.Meanwhile,the PIs show the cutoff wavelengths of 302-356 nm,as well as low moisture absorption(0.30%-0.55%)and low dielectric constant(2.78-3.12 at 1 MHz).

关键词： polyimides tertbutyl group solubility dielectric constant

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Synthesis and Characterization of Fluorinated Polyimide Derived from 3,3＇-Diisopropyl-4,4＇-diaminodiphenyl- 3＂,4＂-difluorophenylmethane

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Chinese Journal of Chemistry 2015年第10期33卷 1159-1166页

作者： xiaohua huang Mei Mei Chanjuan Liu Xianglin Pei Chun Wei Key Laboratory of New Processing Technology for Nonferrous Metal & Materials Ministry of Education andSchool of Material Science and Engineering Guilin University of Technology Guilin Guangxi 541004 China Ministry of Education Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources School of Chemistry and Pharmaceutical Science Guangxi Normal University Guilin Guangxi 541004 China

A novel aromatic diamine monomer, 3,3＇-diisopropyl-4,4＇-diaminodiphenyl-3＂,4＂-difluorophenylmethane （PAFM）, was successfully synthesized by coupling of 2-isopropylaniline and 3,4-difluorobenzaldehyde. The aro- matic diamine was adopted to synthesize a series of fluorinated polyimides by polycondensation with various dian- hydrides： pyromellitic dianhydride （PMDA）, 3,3＇,4,4＇-biphenyltetracarboxylic dianhydride （BPDA）, 4,4＇-oxydiph- thalic anhydride （ODPA） and 3,3＇,4,4＇-benzophenone tetracarboxylic dianhydride （BTDA） via the conventional one-step method. These polyimides presented excellent solubility in common organic solvents, such as N,N-dimeth- ylformamide （DMF）, N,N-dimethyl acetamide （DMAc）, dimethyl sulfoxide （DMSO）, N-methyl-2-pyrrolidone （NMP）, chloroform （CHC13）, tetrahydrofuran （THF） and so on. The glass transition temperatures （Tg） of fluorinated polyimides were in the range of 260--306 ℃ and the temperature at 10% weight loss in the range of 474--502 ℃ Their films showed the cut-off wavelengths of 330--361 nm and higher than 80% transparency in a wavelength range of 385--463 nm. Moreover, polymer films exhibited low dielectric properties in the range of 2.76--2.96 at 1 MHz, as well as prominent mechanical properties with tensile strengths of 66.7--97.4 MPa, a tensile modulus of 1.7--2.1 GPa and elongation at break of 7.2%- 12.9%. The polymer films also showed outstanding hydrophobicity with the contact angle in the range of 91.2°--97.9°.

关键词： fluorinated polyimides solubility transparency dielectric constant

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High Glass Transition of Fluorinated Polyimides Derived from 4-（3,4-Difluorophenyl）-2,6-bis（4-aminophenyl）pyridine

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Chinese Journal of Chemistry 2017年第3期35卷 341-346页

作者： XU Xu Beicai Chen Hua Li xiaohua huang Chun Wei Key Laboratory of New Processing Technology for NonJerrous Metal & Materials Ministry of Education andSchool of Material Science and Engineering Guilin University of Technology Guilin Guangxi 541004 China Department of Mechanical Automotive & Materials Engineering University of Windsor 401 Sunset Ave Windsor ON Canada N9B 3P4

A series of novel fluorinated polyimides (PIs) were prepared from 4‐(3,4‐difluorophenyl)‐2,6‐bis(4‐aminophenyl)pyridine (FPAP) and commercial aromatic dianhydrides via conventional two‐step thermal imidization technique. The structure of the monomer was confirmed by FT‐IR, NMR, and mass spectrometry. All of the obtained PIs were studied with FT‐IR, TGA, DSC, WAXD and contact angles analysis. These results showed that polymer materials presented excellent thermal properties with the glass transition temperature ( T g ) in the range of 336—434 °C, as well as T 5% , and T 10% decomposition temperature ranges of 543—600 °C and 562—622 °C in a nitrogen atmosphere, respectively. Moreover, they presented excellent mechanical properties with tensile strengths of 68—91 MPa, elongation at break of 5.6%—10.2%, and young's modulus of 1.8—2.4 GPa. Furthermore, they showed outstanding hydrophobicity with the contact angle in the range of 90.5°—96.6°, and the results of WAXD indicated all of the polymers presented amorphous structure.

关键词： fluorinated polyimides thermal properties pyridine hydrophobicity

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