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溶酶体相关膜蛋白1在结直肠癌的表达及其与临床预后的相关性

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中国临床研究 2024年第3期37卷 383-387,391页

作者：潘英杨俊荣黄晓丽南京医科大学第四附属医院消化内科江苏南京210031 南京医科大学第四附属医院病理科江苏南京210031

目的检测溶酶体相关膜蛋白1(LAMP1)在结直肠癌(CRC)的表达情况同时研究LAMP1表达与CRC重要临床病理指标,尤其是疾病预后的关系。方法通过生物信息学分析在大数据层面检索LAMP1在CRC的表达情况和预后特性。分别在10例CRC肿瘤组织和包... 详细信息

目的检测溶酶体相关膜蛋白1(LAMP1)在结直肠癌(CRC)的表达情况同时研究LAMP1表达与CRC重要临床病理指标,尤其是疾病预后的关系。方法通过生物信息学分析在大数据层面检索LAMP1在CRC的表达情况和预后特性。分别在10例CRC肿瘤组织和包含60例CRC样本的组织芯片中进行qPCR和免疫组化检测,使用单因素及多因素Cox回归法及Kaplan-Meier生存曲线分析影响CRC预后的因素。结果大数据显示LAMP1蛋白主要定位于溶酶体,在CRC细胞系上有不同程度的高表达,同时在CRC肿瘤组织中显著高于正常对照组织,高表达LAMP1的CRC患者无复发生存率和总生存率均低于低表达LAMP1患者。临床10例qPCR及免疫组化结果均显示CRC中LAMP1表达明显高于癌旁对照组织(Png/mL及远处转移阳性的患者总生存期较短。结论 LAMP1在CRC中存在异常高表达且LAMP1表达与CRC恶性表型明显相关。LAMP1表达可作为CRC预后的预测指标。

关键词：结直肠癌溶酶体相关膜蛋白1 生物信息学免疫组化淋巴转移肿瘤分化远处转移

模拟高放废液硼硅酸盐玻璃固化体钼分相与化学稳定性的结构基因模拟

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硅酸盐学报 2024年第6期52卷 2068-2080页

作者：戴晨李洪张丽艳胡丽丽朱小莉潘安练湖南大学材料科学与工程学院长沙410082中国中国科学院上海光学精密机械研究所高功率激光单元技术实验室上海201800中国日本电气硝子株式会社(NEG)美国分公司北卡罗来纳28150美国

碱金属钼酸盐黄相在高放废液玻璃固化过程中难以处理,严重影响玻璃的化学稳定性。为了对高钼废液固化玻璃的化学稳定性进行较为准确的预判,从而加速配方研发进程,利用玻璃结构基因模拟法(GSg M)研究了高Mo (2.6%~3.3%,质量分数)模拟高... 详细信息

碱金属钼酸盐黄相在高放废液玻璃固化过程中难以处理,严重影响玻璃的化学稳定性。为了对高钼废液固化玻璃的化学稳定性进行较为准确的预判,从而加速配方研发进程,利用玻璃结构基因模拟法(GSg M)研究了高Mo (2.6%~3.3%,质量分数)模拟高放废液硼硅酸盐玻璃的化稳性和钼分相累积情况,建立了B、Li、Na、Mo浸出率、玻璃转变温度和钼分相累积程度的结构预测模型,并进行了模型验证。研究显示,在本工作的成分研究范围内,钼分相成分主要为CsLiMoO_(4)(水溶性黄相)及CaMoO_(4)(耐水性晶相)。成分–结构–性质关系表明,提高Li_(2)O和CaO会显著促进钼分相的形成。518~528 cm^(-1)Si—O—Si振动增强,则钼分相加剧,但[BO_(3)]基团中B—O—B振动增强,可有效减少钼分相的析出。模型验证结果表明,各性质的结构预测模型可对设计玻璃的化稳性及钼分相累积程度进行快速预测,以筛选设计玻璃配方是否有进一步研究的必要。

关键词：高放废液硼硅酸盐玻璃固化钼分相浸出率结构基因模拟

Analysis of robust stability for switched systems with multiple time-delays

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控制理论与应用（英文版） 2011年第2期9卷 283-288页

作者： zh.ng, xiaoli Department of Automation Xiamen University Xiamen Fujian 361005 China

The sufficient conditions of delay-dependent exponential stability for switched systems and robust exponential stability for uncertain switched systems with two time-delays are presented by using average dwell time method and free-weighting matrix *** interaction between different time-delays is *** sufficient conditions do not need that every subsystem is *** designed methods of the switching law are also *** sufficient conditions are given in the form of linear matrix inequalities that can be solved *** result is proven to be valid by the simulation at last.

关键词： Switched systems with multiple time-delays Average dwell time Delay-dependent Robust stability

国产枸橼酸托法替布片的药动学及生物等效性研究

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医药导报 2024年第2期43卷 203-207页

作者：鲁萍王杰尹小丽张顺芝吴蔚中南大学湘雅医学院附属长沙医院、长沙市第一医院Ⅰ期临床研究中心长沙410011 湖南泰新医药科技有限公司长沙410000

目的通过探讨2种枸橼酸托法替布片在健康人群中的药动学特征,评价其生物等效性和安全性。方法采用随机、两周期、自身交叉设计,空腹/餐后各纳入36例受试者,每周期分别服用枸橼酸托法替布片(T或R)5 mg各1片,采用液相色谱-串联质谱(LC-MS/... 详细信息

目的通过探讨2种枸橼酸托法替布片在健康人群中的药动学特征,评价其生物等效性和安全性。方法采用随机、两周期、自身交叉设计,空腹/餐后各纳入36例受试者,每周期分别服用枸橼酸托法替布片(T或R)5 mg各1片,采用液相色谱-串联质谱(LC-MS/MS)法检测血浆中托法替布片的浓度,Phoenix WinNonlin软件进行分析,计算药动学参数,评价其生物等效性。结果受试者单次口服T和R后,空腹组托法替布的主要药动学参数血药峰浓度(C_(max))分别为(57.54±13.95)、(59.17±12.31)ng·mL^(-1);浓度-时间曲线下面积(AUC_(0-t))分别为(143.83±34.58),(142.13±33.00)ng·h·mL^(-1);AUC_(0-∞)分别为(147.39±35.27)、(146.15±34.64)ng·h·mL^(-1);达峰时间(t_(max))均为0.50 h,餐后组托法替布的主要药动学参数C_(max)分别为(57.16±17.56)、(55.19±21.98)ng·mL^(-1);AUC_(0-t)分别为(165.47±41.63)、(162.04±41.84)ng·h·mL^(-1);AUC_(0-∞)分别为(171.88±44.15)、(168.05±44.21)ng·h·mL^(-1);t_(max)均为1.0 h,两制剂空腹组和餐后组C_(max)、AUC_(0-t)、AUC_(0-∞)几何均值比的90%置信区间分别为96.35%(90.11%~103.03%)和105.91%(95.20%~117.83%)、101.02%(98.76%~103.34%)和102.23%(99.67%~104.86%)、100.77%(98.53%~103.06%)和102.40%(99.81%~105.06%),均在80.00%~125.00%内。结论2种枸橼酸托法替布片在中国健康人群中生物等效,并且安全性良好。

关键词：枸橼酸托法替布片药动学生物等效

手术治疗的坏死性小肠结肠炎早产儿并发脑损伤的危险因素分析

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中华小儿外科杂志 2024年第7期45卷 613-618页

作者：徐豆豆殷小利胡佩佩许马利李巍松王杨安徽医科大学第一附属医院儿科合肥230022

目的探讨手术治疗的坏死性小肠结肠炎(necrotizing enterocolitis,NEC)早产儿并发脑损伤的危险因素。方法回顾性分析2016年1月至2022年12月安徽医科大学第一附属医院手术治疗的55例NEC早产儿临床资料,所有患儿中位发病日龄为16(12,25)d... 详细信息

目的探讨手术治疗的坏死性小肠结肠炎(necrotizing enterocolitis,NEC)早产儿并发脑损伤的危险因素。方法回顾性分析2016年1月至2022年12月安徽医科大学第一附属医院手术治疗的55例NEC早产儿临床资料,所有患儿中位发病日龄为16(12,25)d;修正Bell分期为ⅡB期17例(30.9%,17/55),Ⅲ期38例(69.1%,38/55)。依据患儿纠正胎龄近足月时的磁共振成像(magnetic resonance imaging,MRI)评分作为脑损伤的判断指标,将患儿分为MRI+组(发生脑损伤,30例)和MRI-组(未发生脑损伤,25例)。比较两组患儿术前围生期情况、临床特征、实验室检查结果、临床合并症和手术情况等。采用多因素logistic回归分析探讨手术治疗的NEC早产儿发生脑损伤的危险因素。结果MRI+组肠穿孔的发生率(66.7%,20/30)高于MRI-组(36.0%,9/25),差异有统计学意义(P=0.032)。MRI+组发病时血红蛋白水平低于MRI-组[(108.9±17.5)g/L比(129.3±24.2)g/L,P=0.001],降钙素原(procalcitonin,PCT)水平高于MRI-组[18.6(0.4,34.0)ng/ml比1.5(0.8,3.0)ng/ml,P=0.029]。MRI+组病变肠管以多病灶型或广泛病变型为主(60.0%,18/30),MRI-组以局灶型病变居多(72.0%,18/25),差异有统计学意义(P=0.018)。多因素logistic回归分析显示,发病时PCT(OR=1.108,95%CI:1.012~1.214)是手术治疗的NEC早产儿发生脑损伤的独立危险因素,血红蛋白(OR=0.955,95%CI:0.918~0.994)是保护因素。结论发病时PCT是手术治疗的NEC早产儿发生脑损伤的独立危险因素,血红蛋白是保护因素,动态监测PCT和血红蛋白水平有助于评估手术治疗NEC早产儿的神经发育结局。

关键词：小肠结肠炎,坏死性早产儿脑损伤外科手术危险因素

Karacoline,identified by network pharmacology, reduces degradation of the extracellular matrix in intervertebral disc degeneration via the NF-κB signaling pathway

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Journal of Pharmaceutical Analysis 2020年第1期10卷 13-22页

作者： xiaoli zhou Yingying Hong Yulin zhan Department of Traditional Chinese Medicine College of MedicineShanghai University of Traditional Chinese MedicineShanghai201203China Department of Biology School of Fisheries and Life SciencesShanghai Ocean UniversityShanghai201306China Orthopedics Department Shanghai Sixth People’s Hospital East Affiliated to Shanghai University of Medicine&Health ScienceShanghai201306China

Karacoline is a compound found in the plant Aconitum kusnezoffii *** Aconitum kusnezoffii Reichb is widely used for the treatment of pain,very few studies have been carried out on the use of karacoline due to its potential *** this study,we selected key matrix metalloproteinases(MMPs),collagen II,and aggrecan as targets due to their association with intervertebral disc degeneration(IDD).Using these targets,we then used network pharmacology to predict a series of molecules that might exert therapeutic effects on *** these molecules,karacoline was predicted to have the best *** necrosis factor(TNF)-a is known to promote the degeneration of the extracellular matrix in *** therefore applied different concentrations of karacoline(0,1.25,or 12.88 mM)along with 100 ng/mL TNF-a to rat nucleus pulposus cells and found that karacoline reduced the expression of MMP-14 in IDD by inhibiting the nuclear factor(NF)-κB pathway,while collagen II and aggrecan expression was *** suggested that extracellular matrix degradation was inhibited by karacoline(Png novel drugs.

关键词： Karacoline Network pharmacology Intervertebral disc degeneration Extracellular matrix Matrix metalloproteinases

Study on the resonance Rayleigh scattering spectra and resonance non-linear spectra of congo red-amikacin system and its analytical application

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Science China Chemistry 2006年第6期49卷 507-516页

作者： LIU Shaopu HU xiaoli LIU zh.ngfang School of Chemistry and Chemical Engineering Southwest University Chongqing 400715 China School of Chemistry and Chemical Engineering Southwest University Chongqing 400715 China School of Chemistry and Chemical Engineering Southwest University Chongqing 400715 China

The interaction between congo red (CR) and amikacin (AMK) was studied by resonance Rayleigh scattering (RRS), frequency doubling scattering (FDS) and second-order scattering (SOS) combining with absorption spectrum. In a weak acidic medium, CR combined with AMK to form an ion association complex with the composition ratio of 1∶1 by electrostatic interaction, hydrophobicity and charge transferring effect. As a result, the new spectra of RRS, FDS, and SOS appeared and their intensities were enhanced greatly. The maximum wavelengths of RRS, FDS and SOS were located at 563 nm, 475 nm and 940 nm, and the scattering intensities were proportional to the concentration of AMK. These three methods have very high sensitivities, and the detection limits were 4.0 ng·mL?1 for RRS, 3.6 ng·mL?1 for FDS and 1.9 ng·mL?1 for SOS, respectively. At the same time, the methods have better selectivity. A new method for the determination of trace amounts of AMK with congo red by resonance scattering technique has been developed. The recovery for the determination of AMK in blood serum and urine sample was between 95.5% and 105.5%. In this study, the properties, such as enthalpy of formation, charge distribution and mean polarizability, were calculated by AM1 quantum chemistry method. In addition, the reaction mechanism and the reasons for the enhancement of scattering spectra were discussed.

关键词： amikacin, congo red, resonance Rayleigh scattering, second-order scattering, frenquency doubling scattering, resonance non-linear scattering.

梓醇对类风湿性关节炎成纤维滑膜细胞增殖和YAP/TAZ信号通路的影响

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中国细胞生物学学报 2024年第6期46卷 1159-1170页

作者：江忠英向刚周晓莉资阳市雁江区人民医院风湿免疫内分泌科资阳641300

该文探究梓醇对类风湿性关节炎(RA)成纤维滑膜细胞增殖(FLS)和Yes相关蛋白(YAP)/PDZ结合基序转录共激活因子(TAZ)信号通路的影响。体外培养人类风湿关节炎成纤维滑膜细胞HFLS-RA并以20 ng/mL肿瘤坏死因子α(TNF-α)诱导建立RA细胞模型,... 详细信息

该文探究梓醇对类风湿性关节炎(RA)成纤维滑膜细胞增殖(FLS)和Yes相关蛋白(YAP)/PDZ结合基序转录共激活因子(TAZ)信号通路的影响。体外培养人类风湿关节炎成纤维滑膜细胞HFLS-RA并以20 ng/mL肿瘤坏死因子α(TNF-α)诱导建立RA细胞模型,取SD大鼠采用Ⅱ型胶原构建RA动物模型,将其随机分为模型组、梓醇组、空载组、梓醇+YAP过表达组,每组10只,另选10只SD大鼠和正常培养HFLS-RA细胞设为对照组,梓醇、空载质粒和YAP过表达质粒分组处理后以CCK-8法和TUNEL染色、流式细胞术分别检测各组HFLS-RA细胞增殖、凋亡情况;足趾测量仪评测各组大鼠足容积并按5级评分法进行关节炎评分;HE染色检测各组大鼠关节滑膜组织病理形态;TUNEL染色检测各组大鼠关节滑膜组织细胞凋亡情况;酶联免疫吸附测定(ELISA)检测各组HFLS-RA细胞与RA大鼠血清促炎因子白细胞介素-1β(IL-1β)、单核细胞趋化蛋白-1(MCP-1)、IL-6水平;免疫印迹实验检测各组HFLS-RA细胞与RA大鼠关节滑膜组织增殖与YAP/TAZ信号相关蛋白表达情况。结果显示,与对照组相比,模型组HFLS-RA细胞存活率、大鼠足容积及关节炎评分、HFLS-RA细胞与RA大鼠血清IL-6、MCP-1、IL-1β水平、HFLS-RA细胞与RA大鼠关节滑膜组织Cyclin D1、YAP、TAZ蛋白表达水平升高(P0.05)。与梓醇组相比,梓醇+YAP过表达组HFLS-RA细胞存活率、大鼠足容积及关节炎评分、HFLS-RA细胞与RA大鼠血清IL-6、MCP-1、IL-1β水平、HFLS-RA细胞与RA大鼠关节滑膜组织Cyclin D1、YAP、TAZ蛋白表达水平升高(P<0.05),HFLS-RA细胞凋亡率、大鼠关节滑膜组织细胞凋亡比降低(P<0.05)。总之,梓醇可通过减弱YAP/TAZ信号活性而降低RA大鼠及细胞模型炎症水平,进而抑制FLS增殖,并减轻RA大鼠关节炎症状。

关键词：梓醇类风湿性关节炎成纤维滑膜细胞增殖 YAP/TAZ

Interactions of pyrogallol red with proteins and the determination of proteins by resonance light-scattering

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西南师范大学学报(自然科学版) 1998年第5期23卷 67-72页

作者： Hu xiaoli 不详

The interactions of pyrogallol red (PR) with proteins and a derivative method for the determination of proteins were proposed. In the pH range 2 56-4 10, PR interacts with proteins, including bovine serum albumin, human serum albumin, lysozyme, chymotrypsin, resulting in enhanced resonance light scattering(RLS) with the maximal peak at 360 0 nm. With the enhanced RLS, proteins in the range 0-5 0(μg/mL can be determined with the determination limits between 10 3 and 32 2 ng/mL depending on different protein. The effects of foreign substances were tested. The method has been applied to the determination of protein in the synthetic samples and human urine with satisfaction results.

关键词： proteins Pyrogallol Red(PR) resonance light scattering(RLS)