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A novel non-invasive identification of genome editing mutants from insect exuviae

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Insect Science 2022年第1期29卷 21-32页

作者： xiao-xiao wang Jing Li Tong-Xin wang Yi-Nuo Yang Hai-Kang Zhang Meng Zhou Le Kang Li-Ya Wei College of Life Sciences Institute of Life Sciences and Green DevelopmentHebei UniversityBaodingHebei Province071002China State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijing100101China

With the wide application of genome editing in insects, a simple and efficient identification method is urgently needed to meet the increasing demand for mutation detection. Here, taking migratory locusts as a model system, we developed a non-invasive method to accurately identify genome-edited mutants by using DNA from insect exuviae. We compared the quantity and quality of genomic DNA from exuviae in five instar hoppers and found that the 1st instar exuviae had the highest DNA yield and content, while the 3rd instar exuviae had the best quality. Consensus genotypes were identified from genomic DNA of hoppers at different developmental stages in the same individuals. Moreover, we demonstrated that the amplification products from DNA extracted from locust exuviae are the consensus sequences with those from the hemolymph and foreleg pre-tarsus. Therefore, non-invasive samples provide the same genotyping results as minimally invasive and invasive samples of the same individuals. Furthermore, this identification method that uses genomic DNA from exuviae can be used for early screening of positive genome-edited individuals in each generation for adult crossing. In our study, the non-invasive identification method was not only simpler and provided results earlier than existing methods, but also had a better reproducibility and accuracy. This non-invasive identification approach using genomic DNA from exuviae can be adapted to meet the growing demand for genetic analysis and will find wide application in insect genome editing research.

关键词： exuviae genome editing identification locusts molt non-invasive

Computational Experiments for Complex Social Systems:Integrated Design of Experiment System

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IEEE/CAA Journal of Automatica Sinica 2024年第5期11卷 1175-1189页

作者： xiao Xue Xiangning Yu Deyu Zhou xiao wang Chongke Bi Shufang wang Fei-Yue wang College of Intelligence and Computing Tianjin UniversityTianjin 300350China School of Software Shandong UniversityJinan 250101China School of Artificial Intelligence Anhui UniversityHefei 230601China School of Geographic and Environmental Sciences Tianjin Normal UniversityTianjin 300387China Institute of Automation Chinese Academy of Sciences Beijing 100190China

Powered by advanced information industry and intelligent technology,more and more complex systems are exhibiting characteristics of the cyber-physical-social systems(CPSS).And human factors have become crucial in the operations of complex social *** mechanical analysis and social simulations alone are powerless for analyzing complex social *** this backdrop,computational experiments have emerged as a new method for quantitative analysis of complex social systems by combining social simulation(e.g.,ABM),complexity science,and domain ***,in the process of applying computational experiments,the construction of experiment system not only considers a large number of artificial society models,but also involves a large amount of data and *** a result,how to integrate various data,model and knowledge to achieve a running experiment system has become a key *** paper proposes an integrated design framework of computational experiment system,which is composed of four parts:generation of digital subject,generation of digital object,design of operation engine,and construction of experiment ***,this paper outlines a typical case study of coal mine emergency management to verify the validity of the proposed framework.

关键词： artificial system operations

Navigation Finsler metrics on a gradient Ricci soliton

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Applied Mathematics(A Journal of Chinese Universities) 2024年第2期39卷 266-275页

作者： LI Ying MO xiao-huan wang xiao-yang Department of Applied Mathematics Zhejiang University of TechnologyHangzhou 310023China Key Laboratory of Pure and Applied Mathematics School of Mathematical SciencesPeking UniversityBeijing 100871China School of Mathematics and Statistics Beijing Institute of TechnologyBeijing 100081China

In this paper,we study a class of Finsler metrics defined by a vector field on a gradient Ricci *** obtain a necessary and sufficient condition for these Finsler metrics on a compact gradient Ricci soliton to be of isotropic S-curvature by establishing a new integral *** we determine the Ricci curvature of navigation Finsler metrics of isotropic S-curvature on a gradient Ricci soliton generalizing result only known in the case when such soliton is of Einstein *** its application,we obtain the Ricci curvature of all navigation Finsler metrics of isotropic S-curvature on Gaussian shrinking soliton.

关键词： gradient Ricci soliton navigation Finsler metric isotropic S-curvature Ricci curvature Gaussian shrinking soliton

On Minor Left Prime Factorization Problem for Multivariate Polynomial Matrices

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Journal of Systems Science & Complexity 2024年第3期37卷 1295-1307页

作者： LU Dong wang Dingkang xiao Fanghui School of Mathematics Southwest Jiaotong UniversityChengdu 610031China KLMM Academy of Mathematics and Systems ScienceChinese Academy of SciencesBeijing 100190China School of Mathematical Sciences University of Chinese Academy of SciencesBeijing 100049China MOE-LCSM School of Mathematics and StatisticsHunan Normal UniversityChangsha 410081China

A new necessary and sufficient condition for the existence of minor left prime factorizations of multivariate polynomial matrices without full row rank is *** key idea is to establish a relationship between a matrix and any of its full row rank *** on the new result,the authors propose an algorithm for factorizing matrices and have implemented it on the computer algebra system *** examples are given to illustrate the effectiveness of the algorithm,and experimental data shows that the algorithm is efficient.

关键词： Free modules Grobner bases minor left prime(MLP) multivariate polynomial matrices polynomial matrix factorizations

A Mean-Field Game for a Forward-Backward Stochastic System With Partial Observation and Common Noise

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IEEE/CAA Journal of Automatica Sinica 2024年第3期11卷 746-759页

作者： Pengyan Huang Guangchen wang Shujun wang Hua xiao the School of Control Science and Engineering Shandong University the School of Statistics and Mathematics Shandong University of Finance and Economics IEEE the School of Management Shandong University the School of Mathematics and Statistics Shandong University

This paper considers a linear-quadratic(LQ) meanfield game governed by a forward-backward stochastic system with partial observation and common noise,where a coupling structure enters state equations,cost functionals and observation ***,to reduce the complexity of solving the meanfield game,a limiting control problem is *** virtue of the decomposition approach,an admissible control set is *** a filter technique and dimensional-expansion technique,a decentralized control strategy and a consistency condition system are derived,and the related solvability is also ***,we discuss an approximate Nash equilibrium property of the decentralized control ***,we work out a financial problem with some numerical simulations.

关键词： Decentralized control strategy ϵ-Nash equilibrium forward-backward stochastic system mean-field game partial observation

Data-Driven Design for Targeted Regulation of Heat Transfer in Carbon/Carbon Composite Structure

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Journal of Thermal Science 2024年第2期33卷 648-657页

作者： xiao Heye wang Zelin wang Hui JI Ritian Unmanned System Research Institute Northwestern Polytechnical UniversityXi’an710072China School of Aeronautics Northwestern Polytechnical UniversityXi’an710072China Research&Development Institute of Northwestern Polytechnical University in Shenzhen Shenzhen518063China MOE Key Laboratory of Thermo-Fluid Science and Engineering School of Energy and Power EngineeringXi’an Jiaotong UniversityXi’an710049China

Targeted regulation of heat transfer in carbon/carbon composite structure is built for cooling electronic device.A three-dimensional data-driven design model coupling genetic algorithm(GA) with self-adaption deep learning for targeted regulation of heat transfer in built structure is *** self-adaption deep learning model predicts the temperature of built structure closer to optimal value in GA *** distributions of pore and carbon fiber bundles in built structure are optimized by the proposed *** surface temperature of electronic device in the optimized structures is 19.1%-27.5% lower than that in the initial configurations when the porosity of built structure varies from 3% to 11%.The surface temperature of electronic device increases with an increase in *** built structure with carbon fiber bundles near the surface of electronic device and pore distribution in the middle of structure has a higher heat dissipation capacity compared with that in the initial ***,the computation time of the proposed model is less than one tenth compared with that of the traditional genetic algorithm.

关键词： targeted regulation of heat transfer self-adaption deep learning genetic algorithm carbon/carbon composite structure

Characterizations of semihoops based on derivations

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Applied Mathematics(A Journal of Chinese Universities) 2024年第2期39卷 291-310页

作者： wang Mei ZHANG xiao-hong School of Electrical and Control Engineering Shaanxi University of Science and TechnologyXi'an 710021China School of Arts and Sciences Shaanxi University of Science and TechnologyXi'an 710021China School of Mathematics and Statistics Weinan Normal UniversityWeinan 714099China

In this paper,we discuss the related properties of some particular derivations in semihoops and give some characterizations of ***,we prove that every Heyting algebra is isomorphic to the algebra of all multiplicative derivations and show that every Boolean algebra is isomorphic to the algebra of all implicative ***,we show that the sets of multiplicative and implicative derivations on bounded regular idempotent semihoops are in oneto-one correspondence.

关键词： (prelinear)semihoop derivation Heyting algebra Galois connection

Quantitative Analysis of Seeing with Height and Time at Muztagh-Ata Site Based on ERA5 Database

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Research in Astronomy and Astrophysics 2024年第1期24卷 87-95页

作者： xiao-Qi Wu Cun-Ying xiao Ali Esamdin Jing Xu Ze-Wei wang Luo xiao Institute for Frontiers in Astronomy and Astrophysics Beijing Normal UniversityBeijing 102206China Department of Astronomy Beijing Normal UniversityBeijing 100875China Xinjiang Astronomical Observatory Chinese Academy of SciencesUrumqi 830011China University of Chinese Academy of Sciences Beijing 100049China

Seeing is an important index to evaluate the quality of an astronomical *** estimate seeing at the Muztagh-Ata site with height and time quantitatively,the European Centre for Medium-Range Weather Forecasts reanalysis database(ERA5)is *** calculated from ERA5 is compared consistently with the Differential Image Motion Monitor seeing at the height of 12 *** show that seeing decays exponentially with height at the Muztagh-Ata *** decays the fastest in fall in 2021 and most slowly with height in *** seeing condition is better in fall than in *** median value of seeing at 12 m is 0.89 arcsec,the maximum value is1.21 arcsec in August and the minimum is 0.66 arcsec in *** median value of seeing at 12 m is 0.72arcsec in the nighttime and 1.08 arcsec in the *** is a combination of annual and about biannual variations with the same phase as temperature and wind speed indicating that seeing variation with time is influenced by temperature and wind *** Richardson number Ri is used to analyze the atmospheric stability and the variations of seeing are consistent with Ri between *** quantitative results can provide an important reference for a telescopic observation strategy.

关键词： site testing atmospheric effects methods:data analysis telescopes Earth

Metal-organic frameworks on 3D interconnected macroporous sponge foams for large-scale water decontamination:A mini review

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Chinese Chemical Letters 2024年第5期35卷 77-84页

作者： xiao-Hong Yi Chong-Chen wang Beijing Key Laboratory of Functional Materials for Building Structure and Environment Remediation School of Environment and Energy EngineeringBeijing University of Civil Engineering and ArchitectureBeijing 100044China

Water pollution caused by global population growth,urban expansion and industrialization development is one of the urgent issues that need to be addressed in the 21st *** to now,it was challenging for metal-organic frameworks(MOFs)to be used in the actual water treatment due to that the powder MOFs suffered from difficult reuse,poor water stability and easy *** is an effective strategy to immobilize MOFs powder onto porous sponge foam carriers for accomplishing large flux,facile recycling,easy processing water treatment *** this review article,the fabrication approaches and applications of different MOFs/sponge composites were highlighted,in which the fluorescence detection of pollutants,adsorption and separation of pollutants,catalytic reduction and oxidation of pollutants were ***,the future challenges and opportunities of MOF/sponge for water treatment are proposed,aiming to provide in-depth guidance for the future design and manufacture of the immobilized MOFs onto sponge foams.

关键词： Metal-organic frameworks Sponge foam Water decontamination Adsorption Catalysis