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Identification of a novel cytochrome P450 CYP321B1 gene from tobacco cutworm （Spodoptera litura） and RNA interference to evaluate its role in commonly used insecticides

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Insect Science 2017年第2期24卷 235-247页

作者： Rui-Long Wang Keyan Zhu-Salzman Scott R. Baerson xiao-wei xin Jun Li Yi-Juan Su Ren-Sen Zeng Key Laboratory of Tropical Agro-Environment Ministry of Agriculture College of Natural Resources and Environment South China Agri- cultural University Guangzhou China College of Life Sciences Fujian Agriculture and Forestry University Fuzhou China Departments of Entomology Texas A&M University Texas United States Department of Agriculture-Agricultural Research Service Natural Products Utilization Research Unit University Mississippi USA Guangdong Engineering Research Center for Modem Eco-agriculture and Circular Agriculture Guangzhou China

Insect cytochrome P450 monooxygenases （CYPs or P450s） play an important role in detoxifying insecticides leading to resistance in insect populations. A polyphagous pest, Spodoptera litura, has developed resistance to a wide range of insecticides. In the present study, a novel P450 gene, CYP321B1, was cloned from S. litura. The function of CYP321B1 was assessed using RNA interference （RNAi） and monitoring resistance levels for three commonly used insecticides, including chlorpyrifos, β-cypermethrin and methomyl. The full-length complementary DNA sequence of CYP321B1 is 1814 bp long with an open reading frame of 1 488 bp encoding 495 amino acid residues. Quantitative reverse-transcriptase polymerase chain reaction analyses during larval and pupal develop- ment indicated that CYP321B1 expression was highest in the midgut of fifth-instar larvae, followed by fat body and cuticle. The expression of CYP321B1 in the midgut was up- regulated by chlorpyrifos,β-cypermethrin and methomyl with both lethal concentration at 15% （LC15）（50, 100 and 150 μg/mL, respectively） and 50%（LC50） dosages （100,200 and 300μg/mL, respectively）. Addition of piperonyl butoxide （PBO） significantly increased the toxicity ofchlorpyrifos,β-cypermethrin and methomyl to S. litura, suggesting a marked synergism of the three insecticides with PBO and P450-mediated detoxification. RNAi- mediated silencing of CYP321B1 further increased mortality by 25.6% and 38.9% when the fifth-instar larvae were exposed to chlorpyrifos and β-cypermethrin, respectively, at the LCso dose levels. The results demonstrate that CYP321B1 might play an important role in chlorpyrifos and β-cypermethrin detoxification in S. litura.

关键词： CYP321B1 cytochrome P450 monooxygenases insecticide resistance RNAinterference Spodoptera litura

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Intron-Targeted Intron-Exon Splice Conjunction (IT-ISJ) Marker and Its Application in Construction of Upland Cotton Linkage Map

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Agricultural Sciences in China 2008年第10期7卷 1172-1180页

作者： ZHENG Jing ZHANG Zheng-sheng CHEN Li WAN Qun HU Mei-chun WANG wei ZHANG Ke LIU Da-jun CHEN xiao wei xin-qi Key Laboratory of Biotechnology ＆ Crop Quality Improvement Ministry of Agriculture/Southwest University Chongqing 400716 P.R.China

To develop a new DNA maker, which could be used in genetic diversity analysis and genetic map construction in plants, IT-ISJ （intron targeted intron-exon splice junction） primer combinations, which were designed according to the intronexon splice junction conserved sequences, were used to construct cotton genetic linkage map in the present study. 49 out of 704 IT-ISJ primer combinations showed polymorphism between upland cotton high quality cultivar Yumian 1 and multiple dominant gene line T586, and the polymorphic primer combinations accounted for 7.0% of total primer combinations. 49 IT-ISJ primer combinations were used to genotype 270 F2：7 recombinant inbred lines developed from （Yumian 1 × T586） F2, and 58 IT-ISJ loci were obtained. 58 IT-ISJ, together with 150 SSR and 8 morphological loci, were used to conduct linkage analysis, and a linkage map including 22 linkage groups and 113 loci （49 IT-ISJ, 62 SSR, and 2 morphological loci） was constructed. The linkage map covered 714.5 cM with an average interval of 6.3 cM between two markers, accounting for 16.1% of cotton genome. The present study demonstrated that the polymorphism of IT-ISJ marker is high, and it could be effectively applied in plant genetic map construction.

关键词： IT-ISJ (intron targeted intron-exon splice junction) linkage map upland cotton (Gossypium hirsutum L.)

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Nutritional Support During Chemotherapy in a Patient with Advanced Gastric Cancer: A Case Report

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Journal of Nutritional Oncology 2018年第4期3卷 193-197页

作者： xiao wei xin Yu Fang Dong Shao Chen xiao Tian Zhang Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing) Department of Clinical Nutrition Peking University Cancer Hospital and Institute Haidian District Beijing 100000 China Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing) Department of Gastrointestinal Oncology Peking University Cancer Hospital and Institute Haidian District Beijing 100000 China

Gastric cancer (GC) is one of the most common malignancies in China. GC patients often present with dysphagia and abnormal digestive function, leading to malnutrition or even cachexia. In February 2017, an advanced GC patient at Peking University Cancer Hospital received chemotherapy with the additional support of nutritional treatment. After being treated with the dietary prescription and oral nutritional supplements, the patient’s malnutrition was reversed, and he was able to complete five full cycles of chemotherapy.

关键词： Gastric cancer Malnutrition Patient-generated subjective global assessment Chemotherapy Nutritional treatment

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Killing effect of TNF-related apoptosis inducing ligand regulated by tetracycline on gastric cancer cell line NCI-N87

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World Journal of Gastroenterology 2001年第4期7卷 559-562页

作者： xiao-Chao wei xin-Juan Wang Kai-Chen Lei Zhang Yu Liang xin-Li Lin Department of Biochemistry and Molecular Biology,Peking University Health Science Center,Beijing 100083,ChinaProtein Studies,Oklahoma Medical Research Foundation,Oklahoma City,OK 73104,USA Department of Biochemistry and Molecular Biology Peking University Health Science Center 38 Xue Yuan Road Haidian District Beijing 100083 China. xinjuan@***

AIM: To clone the cDNA fragment of human TRAIL (TNF-related apoptosis inducing ligand) into a tetracycline-regulated gene expression system, the RevTet-On system, transduce expression vectors into a gastric carcinoma cell line-NCI-N87 and examine the effects of controlled expression of TRAIL in vitro on the gastric carcinoma cells. METHODS: The full-length cDNA of TRAIL was inserted into a vector under the control of the tetracycline-responsive element (TRE) to obtain the plasmid pRevTRE-TRAIL, which was transfected into a packaging cell line PT67. In addition, vector pRev-Tet On and pRevTRE were also transfected into PT67 separately. After hygromycin and G418 selection, the viral titer was determined. The medium containing retroviral vectors was collected and used to transduce a gastric carcinoma cell line NCI-N87. The resulting cell line NCI-N87-Tet On TRE-TRAIL and a control cell line, NCI-N87 Tet On-TRE, were established. TRAIL expression in the cell line was induced by incubating cells with doxycycline (Dox), which is a tetracycline analogue. The killing effect on gastric carcinoma cells was analyzed after induction. RESULTS: The recombinant plasmid pRev-TRE-TRAIL was constructed. After hygromycin or G418 selection, the producer cell lines PT67-TRE, PT67-TRE-TRAIL and PT67-Tet On were obtained,with titers of about 10(8)CFU.L(-1). By transducing NCI-N87 cells with retroviral vectors from these cell lines, stable cell lines NCI-N87-Tet-On TRE-TRAIL (NN3T) and control cell line NCI-N87-Tet-On-TRE (NN2T) were established. The growth curves of the selected cell lines were the same with the wild type NCI-N87. When Dox was added, cell death was obvious in the test groups (29%-77%), whereas no difference was observed in control and wild type cell lines. With the addition of a medium from the test group, human leukemia cell line Jurkat was activated till death (83%), indicating the secretion of active TRAIL proteins from the test cells to the medium. CONCLUSION: With th

关键词： Stomach Neoplasms 3T3 Cells Animals Anti-Bacterial Agents Apoptosis Apoptosis Regulatory Proteins Doxycycline Gene Expression Regulation, Neoplastic Genetic Vectors Humans Jurkat Cells Membrane Glycoproteins Mice Research Support, Non-U.S. Gov't Retroviridae Transfection Tumor Necrosis Factor-alpha

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SYNTHESES AND PROPERTIES OF 3-METHOXY-DIPHENYLAMINE-4-DIAZONIUM SALTS AND THEIR DIAZO RESINS

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Chinese Science Bulletin 1989年第20期34卷 1709-1712页

作者：曹建伟曹维孝冯新德 Department of Chemistry Peking University

This note reports for the first time the preliminary results concerning the syntheses of 3-methoxy-dephenylamine-4-diazonium salts (M DADS)and the structures and properties of diazo resins produced by them and 4,4′-d... 详细信息

关键词： diazo resins photosensitivity thermostability nucleophilic substitution light sensitive printing plate

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Efficacy of HA330-II column hemoadsorption in Epstein-Barr virusassociated hemophagocytic lymphohistiocytosis combined with liver failure:A case report

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World Journal of Hematology 2022年第1期9卷 6-12页

作者： Qian Gao xiao-wei xin Chun Zhao Yu-Juan Wang wei Wang Yi Yin xiao-Ru Wang You-Peng Jin Pediatric Intensive Care Unit Shandong Provincial HospitalCheeloo College of MedicineShandong UniversityJinan 250021Shandong ProvinceChina Pediatric Intensive Care Unit Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250021Shandong ProvinceChina

BACKGROUND Hemophagocytic lymphohistiocytosis(HLH)is a severe and potentially deadly condition associated with extensive inflammation and immune *** adsorption may serve as a supportive treatment that can stabilize organ function in affected patients by reducing their circulating cytokines *** date,no descriptions of clinical experiences associated with the use of HA330-II column hemoadsorption for the treatment of children affected by HLH have been *** SUMMARY We describe the case of an 11-year-old child with Epstein-Barr virus-associated HLH complicated by liver *** underwent HA330-II column hemoadsorption and chemotherapy and exhibited reductions in levels of inflammatory cytokines,including interleukin(IL),IL-6,IL-8,IL-10,and interferon-γ.The patient’s condition and laboratory parameters gradually improved with *** Hemoadsorption may play an important role in cytokine storm elimination in children with HLH combined with liver failure and consequent multiple organ failure.

关键词： Hemoadsorption HA330-II column Hemophagocytic lymphohistiocytosis Pediatric Liver failure Case report

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Recent progress in constructing fluorinated solid-electrolyte interphases for stable lithium metal anodes

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 270-291页

作者： Di Zhang Pengfei Lv wei Qin xin He Yuanhua He Civil Aircraft Fire Science and Safety Engineering Key Laboratory of Sichuan Province College of Civil Aviation Safety EngineeringCivil Aviation Flight University of ChinaGuanghan 618307China School of Chemical Engineering Sichuan UniversityChengdu 610065China

Lithium metal batteries(LMBs)are emerging as a promising energy storage solution owing to their high energy density and specific ***,the non-uniform plating of lithium and the potential rupture of the solid-electrolyte interphase(SEI)during extended cycling use may result in dendrite growth,which can penetrate the separator and pose significant short-circuit *** a stable SEI is essential for the long-term operation of the ***-rich SEI has garnered significant attention for its ability to effectively passivate electrodes,regulate lithium deposition,and inhibit electrolyte *** the structural components and preparation methods of existing fluorinated SEI is crucial for optimizing lithium metal anode *** paper reviews the research on optimizing LiF passivation interfaces to protect lithium metal *** focuses on four types of compositions in fluorinated SEI that work synergistically to enhance SEI *** instance,combining compounds with LiF can further enhance the mechanical strength and ionic conductivity of the *** metals with LiF significantly improves electrochemical performance at the SEI/anode interface,with a necessary focus on reducing electron tunneling ***,incorporating polymers with LiF offers balanced improvements in interfacial toughness and ionic conductivity,though maintaining structural stability over long cycles remains a critical area for future *** alloys combined with LiF increase surface energy and lithium affinity,challenges such as dendrite growth and volume expansion *** summary,this paper emphasizes the crucial role of interfacial structures in LMBs and offers comprehensive guidance for future design and development efforts in battery technology.

关键词： LiF lithium metal anodes solid-electrolyte interphase interface cycling stability

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深化集体林权制度改革的主要障碍与措施

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经济纵横 2017年第4期 52-56页

作者：肖欣伟黄蕊肖晶长春理工大学计算机科学技术学院吉林长春130022 长春理工大学经济管理学院吉林长春130022

我国新一轮集体林权制度改革已取得丰硕成果,但在改革过程中依然面临林权流转不规范、林业服务体系不健全、林业金融支持力度不够和林业采伐限额管理制度不完善等障碍。总体看,应在经济先发地区推广林地"均股均利"政策,在经济后发地区... 详细信息

我国新一轮集体林权制度改革已取得丰硕成果,但在改革过程中依然面临林权流转不规范、林业服务体系不健全、林业金融支持力度不够和林业采伐限额管理制度不完善等障碍。总体看,应在经济先发地区推广林地"均股均利"政策,在经济后发地区继续推行分林到户政策及构建林业发展和森林碳汇联合组织。在此基础上,进一步规范集体林权流转,完善林业服务体系,强化林业金融支持体系建设,规范林木采伐限额管理。

关键词：集体林权制度改革森林碳汇林地流转

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Electrochemical conversion of methane to bridge the gap in the artificial carbon cycle

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Journal of Energy Chemistry 2025年第1期100卷 286-308页

作者： Yuhao Peng Yuefeng Song Ihar Razanau Juanxiu xiao wei xiao Di Hu Guoxiong Wang College of Chemistry and Molecular Sciences Hubei Key Laboratory of Electrochemical Power Sources Wuhan University State Key Laboratory of Catalysis Dalian National Laboratory for Clean Energy iChEM (Collaborative Innovation Center of Chemistry for Energy Materials) Dalian Institute of Chemical Physics Chinese Academy of Sciences Laboratory of Physical-Chemical Technologies SSPA Scientific and Practical Materials Research Centre of NAS of Belarus State Key Laboratory of Marine Resources Utilization in South China Sea Collaborative Innovation Center of Marine Science and Technology School of Marine Science and Engineering Hainan University Department of Chemical and Environmental Engineering University of Nottingham Ningbo China

Methane, an abundant one-carbon（C1） resource, is extensively used in the industrial production of vital fuels and value-added chemicals. However, current industrial methane conversion technologies are energy-and carbon-intensive, mainly due to the high activation energy required to break the inert C–H bond, low selectivity, and problematic side reactions, including CO2emissions and coke deposition. Electrochemical conversion of methane（ECM） using intermittent renewable energy offers an attractive solution, due to its modular reactor design and operational flexibility across a broad spectrum of temperatures and pressures. This review emphasizes conversion pathways of methane in various reaction systems, highlighting the significance and advantages of ECM in facilitating a sustainable artificial carbon cycle. This work provides a comprehensive overview of conventional methane activation mechanisms and delineates the complete pathways of methane conversion in electrolysis contexts. Based on surface/interface chemistry, this work systematically analyzes proposed reaction pathways and corresponding strategies to enhance ECM efficiency towards various target products, including syngas, hydrocarbons, oxygenates, and advanced carbon materials. The discussion also encompasses opportunities and challenges for the ECM process, including insights into ECM pathways, rational electrocatalyst design, establishment of benchmarking protocols, electrolyte engineering, enhancement of CH4conversion rates, and minimization of CO2emission.

关键词： Methane Electrochemical conversion Reaction mechanism Catalyst design Electrode Electrocatalysis

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Evolution of 4-Coumarate:Coenzyme A Ligase Gene (4CL) and Divergence of Larix(Pinaceae)

Evolution of 4-Coumarate:Coenzyme A Ligase Gene (4CL) and Di...

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第七届全国系统与进化植物学青年学术研讨会

作者： wei xiao-xin, Wang xiao-Quan (Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China)

The evolutionary dynamics of 4-coumarate:coenzyme A ligase gene (4CL) was investigated in Larix based on copy number, GC content and codon usage, sequence divergence and phylogenetic analysis with two Pseudotsuga species as outgroups. All 4CL clones of Larix formed a strongly supported monophyletic group, in which two solid clades (4clA and 4clB) were resulted from an ancient gene duplication event in the common ancestor of Larix. The 4clA clade was further divided into subclades 4clA1 and 4clA2. Frequent duplication/deletion should be one important evolution

关键词： 4CL molecular evolution biogeography Larix Pseudotsuga

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