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Experimental study on the treatment of rabbit corneal melting after alkali burn with Collagen cross-linking

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International Journal of Ophthalmology(English edition) 2012年第2期5卷 147-150页

作者： xiao-wei gao, Ying Liu Ophthalmic Center No.474 Hospital of Chinese PLA Urumchi 830013 Xinjiang Uygur Autonomous Region China The Medical College of Shihezi University Shihezi 832002 Xinjiang Uygur Autonomous Region China

AIM: To evaluate the effect of Collagen cross-linking on the prevention of melting in rabbit corneas after alkali burn. METHODS: Twenty New Zealand white rabbits were randomly divided into model control group and collagen cross-linking treatment group. The second group of rabbits received collagen cross linked treatment. Both groups were applied with antibiotic eye drops to prevent infection. The corneas were evaluated for melting, opacity, pathological and immunohistochemistry, record the changes when 28 days after the animals were killed. RESULTS: In the control group, 6 out of 8 rabbits showed corneal melting after injury (14 +/- 4) days, while two corneal perforated. In collagen cross-linking treatment group, one rabbit showed corneal melting after injury 23 days, without corneal perforation; corneal dissolution rate between the two groups was significantly different (P 0.05). Pathological examination suggested that in the treatment group, mild corneal edema, mild damage to collagen fibers, inflammatory cell infiltration was significantly less than the control group. Immunohistochemistry showed that corneal collagen fibers arranged in neat rows in the control group. CONCLUSION: Collagen cross-linking treatment not only can prevent and delay the corneal melting after alkali burn, but also can reduce the destruction of corneal collagen fibers and infiltration of inflammatory cells in the corneal tissue.

关键词： collagen cross-linking corneal alkali burn corneal melting rabbit

Adhesion of cavernous hemangioma in the orbit revealed by CT and MRI:analysis of 97 cases

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International Journal of Ophthalmology(English edition) 2011年第2期4卷 195-198页

作者： Yan-Ming Tian, xiao-wei gao Chinese PLA Eye Center No. 474 Hospital of Chinese PLA Urumchi 830012 Xinjiang Uygur Autonomous Region China Institute of Orbital Diseases General Hospital of Armed Police Beijing100039 China

AIM: To assess features of cavernous hemangioma (CH) in the orbit revealed by CT and MRI and summarize prediction of preoperative CT and MRI for the adhesion degree of CH in the orbit. METHODS: A total of 97 patients with pathologically confirmed CH in the orbit were examined with axial and coronal CT scan, and axial, coronal, sagittal, and enhanced fat suppression MRI scan. CT and MRI findings and intraoperative adhesion degrees were retrospectively analyzed. RESULTS: There were 47 patients with slight adhesion, for whom CT and MRI showed round masses with well defined margins in the extraocular muscles; 14 patients with mild adhesion, for whom CT and MRI revealed irregular masses with unclear boundary between CH and the optic nerve in coronal images, and emissary veins in the posterior region of masses in contrast-enhanced images; 36 patients with severe adhesion, for whom CT and MRI exhibited an irregular or ovoid mass filling the orbital apex, or showed distorted and even spiky margins in the posterior region of masses in contrast enhanced images at the presence of a transparent triangle between the mass and the orbital apex. CONCLUSION: Preoperative CT and MRI aid in accurate diagnosis, selection of the surgical approach, and assessment of the adhesion degree and surgical risks for CH.

关键词： cavernous hemangioma computerized tomography magnetic resonance imaging

Effects ofminocycline on expression of bcl-2,bax in early retinal neuropathy of diabetes in rats

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International Journal of Ophthalmology(English edition) 2011年第2期4卷 162-164页

作者： Hai-Dong Lian, xiao-wei gao Department of Ophthalmology the First Affiliated Hospital of Medical College of Shihezi University Shihezi 832000 Xinjiang Uygur Autonomous Region China Department of Ophthalmology No. 474 Hospital of Chinese PLA Urumqi 830013 Xinjiang Uygur Autonomous Region China

AIM: To study the effect of the minocycline (MNC) on expression of bcl-2, bax in retinal nerve cells of rat with diabetes. METHODS: Male SD rats were randomly divided into negative control group, model control group and MNC treated group. Diabetes model was established by intraperitoneal injection of 60 mg/kg streptozotocin (STZ). The protein expressions of bcl-2 and bax in retina were detected by immunohistochemistry method. RESULTS: Compared with the negative control group, box immunoreactive neurons in retina were increased significantly (P 0.01) in model control group. However, bax immunoreactive neurons in retina in MNC treated group were significantly decreased (P 0.01). Compared with the model control group, bcl-2 immunoreactive neurons in retina were increased significantly(P 0.01) in MNC treated group. CONCLUSION: MNC can obviously decreased expression of bax and increased expression of bcl-2 in retina with DR. It is one of path of inhibiting impairment on retinal nerve cells with DR.

关键词： mincycline bcl-2 bax retina diabetes

Posterior chamber phakic intraocular lens implantation for high myopia

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International Journal of Ophthalmology(English edition) 2013年第6期6卷 831-835页

作者： Yan Ju xiao-wei gao Bing Ren Department of Ophthalmology No.474 Hospital of Chinese PLA

AIM:To evaluate the efficacy,safety and stability of posterior chamber phakic intraocular lens implantation for the correction of high ***:Retrospective case review of 82 eyes(43patients)undergoing implantable Coliamer lens(ICL)placement by a single surgeon(xiao-wei gao)to correct preoperative mean spherical equivalents between-9.00diopter(D)*** outcome measures included uncorrected visual acuity(UCVA),refraction,best spectacle-corrected visual acuity(BSCVA),endothelial cell density(ECD),intraocular pressure(IOP),lens transparency,postoperative *** anterior segment optical coherence tomography(AS-OCT)was used to measure anterior chamber depth(ACD)and the position of ***:Mean follow-up was 6.54±3.26 months(range 3-12 months).Predictability of the manifest spherical equivalent(SE)refraction to within±*** was achieved in 88%of eyes and±0.50D in 72.5%of *** mean postoperative manifest SE refraction was-1.85±0.72D,with 96.34%of eyes maintaining or gaining≥1 line(s)of *** mean 3-month postoperative ECD decreased but had no statistically difference compared with the preoperative *** the 7 eyes(8.54%)with a mild transient increase in intraocular pressure(up to 30mmHg),none required a second surgical procedure or prolonged topical *** was no loss of lens *** precipitates were observed in 5 eyes(6.09%).The mean preoperative ACD measured with AS-OCT was 3.28±0.14mm,three months after surgery,the mean ACD was2.45±*** chamber depth showed astatistically significant *** eye(1.22%)had ICL spontaneous rotation,81 eyes(98.78%)of the lens remained correctly ***:The implantation of ICL is an effective surgical option for the management of high *** its long time effect and safety still need more time to prove.

关键词： phakic intraocular lens lens implantation myopia

Experimental study of trabecular tissue repair for corneal defect in rabbits

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International Journal of Ophthalmology(English edition) 2020年第9期13卷 1356-1360页

作者： Xia Li Yun xiao xiao-wei gao Yi Liu Dai-Kun Lei Ophthalmology Center of the Army General Hospital of Xinjiang Military RegionUrumchi 830013Xinjiang Uygur Autonomous RegionChina

AIM:To investigate the mechanism and effect of trabecular tissue repair for corneal defect,and to provide a theoretical basis for its clinical ***:Trabeculectomy was performed on 40(80 eyes)of 70 New Zealand white *** trabecular tissue for ***(30 eyes)corneal defect models were made,trabecular tissue was filled in the corneal defect,and the oblique cross stitch was used to suture the corneal laceration and *** segment image and optical coherence tomography(OCT)were performed at the time 1 d,1 wk,1 and 3 mo after the model was *** the observation,the cornea was to ken and stained with trypanosome blue-alizarin red and the pathological tissue was ***:Observation 1 wk after surgery,the area of corneal defect was edema,but the corneal curvature was basically normal,and the anterior chamber existed under slit *** 3 mo of observation,most corneal defects were repaired in the form of corneal leucoma and corneal macula(73.3%),the filled trabecular tissue gradually became transparent,fused tightly with the corneal tissue,and the corneal curvature was relatively *** in one case,the trabecular planter was partially detached,no serious complications such as corneal laceration occurred after the stitches were ***:The trabecular tissue structure is similar to the corneal,and it can be used as a substitute for the corneal tissue defect by providing fiber scaffolds and cell amplification differentiation,and lay a foundation for the second-stage surgical treatment.

关键词： corneal defect trabecular tissue rabbit

Comparative study of modified and conventional secondary hydroxyapatite orbital implantations

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International Journal of Ophthalmology(English edition) 2013年第5期6卷 646-649页

作者： Yong Zhao Mao-Nian Zhang Yun-Xian gao xiao-wei gao Bing Ren Department of Ophthalmology Chinese PLA General Hospital Department of Ophthalmology the Affiliated Hospital of Xinjiang Medical University of TCM Department of Ophthalmology Chinese PLA 474th Hospital

AIMTo compare the clinical effects of the modified and conventional secondary hydroxyapatite orbital implantations.

关键词： hydroxyapatite orbital implantation orbit

Effects of aminoguanidine on retinal apoptosis in mice with oxygen-induced retinopathy

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International Journal of Ophthalmology(English edition) 2013年第4期6卷 436-441页

作者： An-Jie Du Bing Ren xiao-wei gao Lei Yang Yan Fu Xu-Dong Zhao Ophthalmic Center No.474 Hospital of Chinese PLA Department of Ophthalmology Yuncheng Central Hospital Department of Ophthalmology Baoding First Central Hospital

AIM:To explore the protective effects of amino-guanidine(AG) on retinal apoptosis in mice with oxygeninduced retinopathy(OIR).·METHODS:A total of 80 C57BL/6J mice,aged 7 days,were randomly divided into four groups:normal,high oxygen,high oxygen saline and high oxygen treated with *** the normal group,mice were housed in normoxic conditions from postnatal day P7 to *** in the other 3 groups were placed under hyperoxic conditions(75 ±2% O2) in an oxygen-regulated chamber for 5 days and subsequently placed in normoxic conditions for *** in the AG group were treated once daily,from P12 to P17,with AG hemisulfate(100mg/kg body weight,intraperitoneally) dissolved in physiological *** equivalent amount of 0.9% physiological saline was administered,as above,to mice in the high oxygen saline *** mice were randomly selected from each group on P14 and on P17,euthanized and the retinas *** cells in the retina were detected using the terminal-deoxynucleoitidyl transferase mediated nick end labeling(TUNEL) *** expression of nitric oxide synthase(iNOS) in the retina was detected by immunohistochemistry and changes in rod cells were observed using electron microscopy.·RESULTS:TUNEL-positive cells and iNOS immunoreactive neurons were present in the inner nuclear and ganglion cell retinal layers of mice in the high oxygen *** number of TUNEL-positive cells was significantly greater in the high oxygen group compared with the normal group(t =-20.81,P14d<0.05;t =-15.05,P17d<0.05).However,the number of TUNEL-positive cells in the AG treatment group was significantly lower(t =-13.21,P14d<0.05;t =-6.61,P17d<0.05) compared with thehigh oxygen *** expression of iNOS was significantly higher in the high oxygen group compared with the normal group(t =-21.95,P14d<0.05;t =-17.30,P17d<0.05).However,the expression of iNOS in the AG treatment group was significantly lower(t =-12.17,P14d<0.05;t =-10.30,P17d<0.05) com

关键词： aminoguanidine retinopathy of prematurity apoptosis inhibitor of nitric oxide synthase

Mechanism of immune tolerance induced by donor derived immature dendritic cells in rat high-risk corneal transplantation

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International Journal of Ophthalmology(English edition) 2013年第3期6卷 269-275页

作者： xiao-wei gao Yan Fu Wen-Jing Li An-Jie Du Xia Li Xu-Dong Zhao Ophthalmic Center No. 474 Hospital of Chinese PLA Department of Ophthalmology Medical College of Shihezi University

·AIM: To study the role of immature dendritic cells (imDCs) on immune tolerance in rat penetrating keratoplasty (PKP) in high -risk eyes and to investigate the mechanism of immune hyporesponsiveness induced by donor-derived imDCs. ·METHODS: Seventy-five SD rats (recipient) and 39 Wistar rats (donor) were randomly divided into 3 groups: control, imDC and mature dendritic cell (mDC) group respectively. Using a model of orthotopic corneal transplantation in which allografts were placed in neovascularized high -risk eyes of recipient rat. Corneal neovascularization was induced by alkaline burn in the central cornea of recipient rat. Recipients in imDC group or mDC group were injected donor bone marrow-derived imDCs or mDCs of 1 ×10 6 respectively 1 week before corneal transplantation tail vein. Control rat received the same volume of PBS. In each group, 16 recipients were kept for determination of survival time and other 9 recipients were executed on day 3, 7 and 14 after transplantation. Cornea was harvested for hematoxylin eosin staining and acute rejection evaluation, Western blot was used to detect the expression level of Foxp3. ·RESULTS: The mean survival time of imDC group was significantly longer than that of control and mDC groups (all <0.05). The expression level of Foxp3 on CD4 + CD25 + T cells of imDC group (2.24 ±0.18) was significantly higher than that in the control (1.68 ±0.09) and mDC groups (1.46±0.13) (all <0.05).·CONCLUSION: Donor -derived imDC is an effective treatment in inducing immune hyporesponsiveness in rat PKP. The mechanism of immune tolerance induced by imDC might be inhibit T lymphocytes responsiveness by regulatory T cells. ·

关键词： immature dendritic cell high-risk keratoplasty regulatory T cells

Inhibition of retinopathy of prematurity in rat by intravitreal injection of sorafenib

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International Journal of Ophthalmology(English edition) 2014年第2期7卷 198-204页

作者： Li-Li Tian Bing Ren xiao-wei gao Ying Luo Yan Cai Kun Zhou An-Jie Du Yong Zhao Medical College of Shihezi University Ophthalmic Center No.474 Hospital of Chinese PLA Department of Ophthalmology Yuncheng Central Hospital

AIM:To investigate the effect of intravitreal injection administered sorafenib,a multikinase inhibitor,in a rat model of oxygen-induced retinopathy(OIR).METHODS:Seven-day-old Sprague-Dawley rats(n=144)were randomly assigned to six *** A received normal partial oxygen pressure and groups B,C,D,E and F were exposed to hyperoxia(75±2)%from postnatal 7d(P7)to P12 to induce retinopathy of *** rats in groups C,D,E and F were received intravitreal injections of either vehicle(DMSO)or sorafenib at P12(5,20 and 80μg,respectively).Then they returned to normoxia after *** retinas were whole-mounted and imaged with a confocal *** vascular branching points were counted to quantify neovascularization at ***-sections of the retina were stained with hematoxylin and eosin(HE).The nuclei of new vessels breaking the internal limiting membrane were counted to quantify the proliferative neovascular ***:The retinal vessel in groups B and C turned into tortuosity and a great deal of neovascularization were ***-treated rats had significantly less neovascularization as compared with vehicle-treated and control rats in a dose dependent manner(P<0.05).The number of vascular branching points in A,B,C,D,E and F were 16.50±3.90,37.44±6.47,37.08±5.10,30.80±6.85,26.08±5.08 and 19.83±3.51,*** number of the nuclei of retinal new vessel in A,B,C,D,E and F were 0.22±0.42,35.66±4.70,35.30±4.54,27.30±4.28,21.41±3.53,and 7.41±2.87,*** were significant difference between each group(P<0.05)except groups B and ***:In the rat OIR model,sorafenib could inhibit retinal neovascularization in a dose dependent manner.

关键词： sorafenib retinopathy of prematurity neovascularization tyrosine kinase inhibitors