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Rapid detection of Pseudomonas aeruginosa by cross priming amplification

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Journal of Integrative Agriculture 2020年第10期19卷 2523-2529页

作者： XIANG Yong YAN ling ZHENG xiao-cui LI Li-zhen LIU Peng CAO Wei-sheng College of Veterinary Medicine South China Agricultural UniversityGuangzhou 510642P.R.China Key Laboratory of Zoonosis Prevention and Control of Guangdong Province South China Agricultural UniversityGuangzhou 510642P.R.China National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control South China Agricultural UniversityGuangzhou 510642P.R.China South China Collaborative Innovation Centre for Prevention and Control of Poultry Infectious Diseases and Safety of Poultry Products South China Agricultural UniversityGuangzhou 510642.P.R.China

Pseudomonas aeruginosa(PA)is an opportunistic pathogen of humans and animals and a common source of nosocomial infections especially of the respiratory *** aeruginosa is also a major bacterial disease of poultry and in particular,eggs and newly hatched *** this study,we developed a simple,accurate and rapid molecular detection method using cross priming amplification(CPA)with a nucleic acid test strip to detect *** assay efficiently amplified the target gene within 45 min at 62℃only using a simple water *** detection limit of the method was 1.18x 102 copiesμL^-1 for plasmid DNA and 4.4 CFU mL^-1 for bacteria in pure culture,and was 100 times more sensitive than conventional *** screened 83 clinical samples from yellow-feather broiler breeder chickens and hospitalized/treated dogs and cats using CPA,PCR and traditional culture *** positive sample ratios were 15.3%(13/83)by CPA,13.3%(11/83)by PCR and 12.1%(10/83)by the culture *** established CPA method has significant advantages for detecting *** method is easy to use and possesses high specificity and sensitivity without the requirements of complicated experimental *** PA-CPA assay is especially fit for outdoor and primary medical units and is an ideal system for the rapid detection and monitoring of ***.

关键词： Pseudomonas aeruginosa cross priming amplification isothermal amplification rapid detection detection method

Accumulation behavior of heavy metals by Bidens pilosa *** metallurgical slag:effects on plant physiology and absorption characteristics

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Journal of Mountain Science 2023年第9期20卷 2580-2591页

作者： WEI Meng SUN Zheng cui Bu-li HE Yang DONG Zhi-cheng MENG ling-xiao School of Resources and Environmental Engineering Ludong UniversityYantai 264025China Henan Jiuzhou Environmental Protection Engineering Co.LTD Nanyang 473000China School of Geographic Sciences and Tourism Jiaying UniversityMeizhou 514015China

Many of the abandoned mining and industrial land in villages and towns are seriously polluted by heavy metals in China,it is necessary for sustainable development to adopt efficient and economical ways to restore the ecology of abandoned mining and industrial *** level of topsoil contaminated with metallurgical slag from nonferrous metal smelting waste site in Baoding,North China and the heavy metals(HMs)accumulation behavior of Bidens pilosa L.(***,native pioneer plant)were *** selected study sites were mainly contaminated by As(270~434 mg/kg),Cd(63~95 mg/kg),Pb(5496~24504 mg/kg)and Zn(4500~21300 mg/kg),which exceed the national standard *** of multi-metal accumulation in different parts of *** indicated that the absorption of toxic metals varied by types,concentration and species of HMs under stress conditions,soil property and plant *** results showed that *** had excellent ability to accumulate HMs under different HMs stress condition,with the highest accumulation concentration of 85 mg/kg for As,380 mg/kg for Cd,4000 mg/kg for Pb,and 7500 mg/kg for Zn in roots under experimental conditions,*** growth trend of *** declined with the increase of HMs stress concentration in tested *** stress led to different degrees of plant toxicity and obstruction of physiological *** the plant physiological index,Chla and ChlT decreased 28.0%and 28.1%,37.3%and 35.5%under different stress treatments,*** related to physiological metabolic strength and stress resistance of plant,such as MDA(Malondiadehyde),CAT(catalase),SOD(superoxide dismutase)and SP(soluble protein),all increased with the increase of HMs stress concentration.

关键词： Native pioneer plant Heavy metals stress B.pilosa Absorption characteristics Metallurgy waste site

Cross-linked lipoic acid nanoparticles with indole-3-methanol loading for the PTEN-me diate d TNBC treatment

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Journal of Materials Science & Technology 2024年第14期181卷 198-208页

作者： xiao xiao Rong cui Chunyan Liao Shiyong Zhang College of Biomedical Engineering and National Engineering Research Center for Biomaterials Sichuan UniversityChengdu 610064China

We here report a non-gene therapy anti-tumor nanoparticles(I3C@cLANPs)based PTEN by loading indole-3-methanol(I3C)into the cross-linked lipoic acid nanoparticles(cLANPs)for triple-negative breast cancer(TNBC)treatment.I3C is a PTEN-specific natural activator while the poor PTEN-activation effi-ciency impedes its ability to achieve the PTEN-mediated tumor *** to the structural homology of lipoic acid(LA),the cLANPs hold not only LA-like anticancer activity but also PTEN-activation proper-ties,which would synergistically potentiate the PTEN-activation *** in vitro and in vivo data showed that PTEN expression in the I3C@cLANPs group was 2.1 and 2.8 times higher than that of I3C,*** antitumor evaluation based on the 4T1 tumor-bearing mice showed a tumor inhibitory rate(TIR)of 78.4%at the I3C usage of 20 mg kg^(–1),54.5%higher than that of I3C alone and 19.7%higher than that of first-line chemotherapy drug Doxorubicin hydrochloride(DOX).Thus,the I3C@cLANPs could address the low activation efficiency in the PTEN-mediated tumor strategy and avoid the risks of gene therapy,holding a good prospect for TNBC and related cancer treatment.

关键词： Triple-negative breast cancer Indole-3-methanol Lipoic acid Synergistic therapy

TLD calibration and absorbed dose measurement in a radiation-induced liver injury model under a linear accelerator

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Nuclear Science and Techniques 2023年第4期34卷 69-78页

作者： Hui-Hui xiao ling-ling Liu Wen-Yi Li Bing-Bing Li Xiang-Li cui Jie Li Tao-Sheng Li Zong-Tao Hu School of Basic Medical Sciences Anhui Medical UniversityHefei 230000China Hefei Cancer Hospital Chinese Academy of SciencesHefei 230000China Institute of Health and Medical Technology Hefei Institute of Materials ScienceChinese Academy of SciencesHefei 230000China University of Science and Technology of China Hefei 230000China Hefei Institute of Materials Science Chinese Academy of SciencesHefei 230000China

The application of a thermoluminescent detector(TLD) for dose detection at the liver irradiation site in mice under linear accelerator precision radiotherapy and the use of a single high dose to irradiate the mouse liver to construct a biological model of a radiation-induced liver injury(RILD) in mice were to determine the feasibility of constructing a precision radiotherapy model in small animals under a linear accelerator. A 360° arc volumetric rotational intensity-modulated radiotherapy(VMAT) plan with a prescribed dose of 2 Gy was developed for the planned target volume(PTV) at the location of the TLD within solid water to compare the difference between the measured dose of TLD and the assessed parameters in the TPS system. The TLD was implanted in the livers of mice, and VMAT was planned based on TLD to compare the measured and prescribed doses. C57BL/6 J mice were randomly divided into control and 25-Gy radiation groups and were examined daily for changes in body weight. They were euthanized at 3 and 10 weeks after radiation, and the levels of liver serum enzymes such as alanine aminotransferase(ALT), aspartate aminotransferase(AST), and alkaline phosphatase(ALP) were measured to observe any pathological histological changes in the irradiated areas of the mouse liver. The measured values of solid underwater TLD were within ± 3% of the Dmean value of the evaluation parameter in the TPS system. The mice in the 25-Gy radiation group demonstrated pathological signs of radiation-induced liver injury at the site of liver irradiation. The deviation in the measured and prescribed doses of TLD in the mouse liver ranged from-1.5 to 6%;construction of an accurate model of RILD using the VMAT technique under a linear accelerator is feasible.

关键词： Thermoluminescent detector Radiation-induced liver injury Linear accelerator Volumetric rotational intensity-modulated radiotherapy

Local Dependence Test Between Random Vectors Based on the Robust Conditional Spearman's ρ and Kendall's τ

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Acta Mathematicae Applicatae Sinica 2023年第3期39卷 491-510页

作者： ling-yue ZHANG Heng-jian cui School of Mathematical Sciences Capital Normal UniversityBeijing 100048China

This paper introduces two local conditional dependence matrices based on Spearman'sρand Kendall'sτgiven the condition that the underlying random variables belong to the intervals determined by their *** robustness is studied by means of the influence functions of conditional Spearman'sρand Kendall'sτ.Using the two matrices,we construct the corresponding test statistics of local conditional dependence and derive their limit behavior including consistency,null and alternative asymptotic *** studies illustrate a superior power performance of the proposed Kendall-based *** data analysis with proposed methods provides a precise description and explanation of some financial phenomena in terms of mathematical statistics.

关键词： local dependence influence function rank correlation U-statistic

Matched MnO@C anode and porous carbon cathode for Li-ion hybrid supercapacitors

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Rare Metals 2023年第6期42卷 1959-1968页

作者： cui-Hua An Yue-Qing Li Shuai Wu ling-xiao Gao Li-Yang Lin Qi-Bo Deng Ning Hu Key Laboratory of Hebei Province on Scale-Span Intelligent Equipment Technology and School of Mechanical EngineeringHebei University of TechnologyTianjin300401China Wuhan Green Power Hydrogen Energy Technology Co. LTDWuhan430000China The Green Aerotechnics Research Institute of Chongqing Jiaotong University Chongqing401120China State Key Laboratory of Reliability and Intelligence Electrical Equipment Hebei University of TechnologyTianjin300130China Institute of Materials Physics and Technolgy Hamburg University of TechnologyHamburg21073Germany

Holding a promise of achieving high power and energy densities,Li-ion hybrid electrochemical capacitors present a crucial future direction for energy storage ***,technical challenges remain to appropriately couple both high-power capacitor-type and high-energy battery-type electrode materials within a same *** addition,the current electrode materials in the device are usually prepared separately,which leads to lengthy preparation,time-consuming and *** this work,we report a simple method to prepare porous carbon materials(PC) with and without MnO nanoparticle cores,which function as very unique anode/cathode pairs for very high-performance Li ion hybrid *** the respective merits of high Li storage capacity from the MnO@C anode and high-rate performance from the PC cathode,the resulted device exhibits a remarkable energy density of 89 Wh·kg^(-1)(at 48 W·kg^(-1)) and can reach a battery-inaccessible power density of 18 kW·kg^(-1)(at 45Wh·kg^(-1)).

关键词： Li-ion hybrid supercapacitors Structural affinity MnO@C Porous carbon(PC) High energy density

Comparative proteomic analysis of plasma exosomes reveals the functional contribution of N-acetyl-alpha-glucosaminidase to Parkinson’s disease

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Neural Regeneration Research 2025年第10期20卷 2998-3012页

作者： Yuan Zhao Yidan Zhang Xin Liu Jian Zhang Ya Gao Shuyue Li cui Chang Xiang Liu Guofeng Yang Department of Geriatrics Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina Department of Neurosurgery The Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina

Parkinson’s disease is the second most common progressive neurodegenerative disorder,and few reliable biomarkers are available to track disease *** proteins,DNA,mRNA,and lipids carried by exosomes reflect intracellular changes,and thus can serve as biomarkers for a variety of *** this study,we investigated alterations in the protein content of plasma exosomes derived from patients with Parkinson’s disease and the potential therapeutic roles of these proteins in Parkinson’s *** a tandem mass tag-based quantitative proteomics approach,we characterized the proteomes of plasma exosomes derived from individual patients,identified exosomal protein signatures specific to patients with Parkinson’s disease,and identified N-acetyl-alpha-glucosaminidase as a differentially expressed protein.N-acetyl-alpha-glucosaminidase expression levels in exosomes from the plasma of patients and healthy controls were validated by enzyme-linked immunosorbent assay and western *** results demonstrated that the exosomal N-acetyl-alpha-glucosaminidase concentration was not only lower in Parkinson’s disease,but also decreased with increasing Hoehn-Yahr stage,suggesting that N-acetyl-alpha-glucosaminidase could be used to rapidly evaluate Parkinson’s disease ***,western blot and immunohistochemistry analysis showed that N-acetyl-alpha-glucosaminidase levels were markedly reduced both in cells treated with 1-methyl-4-phenylpyridinium and cells overexpressingα-synuclein compared with control ***,N-acetyl-alpha-glucosaminidase overexpression significantly increased cell viability and inhibitedα-synuclein expression in 1-methyl-4-phenylpyridinium-treated *** together,our findings demonstrate for the first time that exosomal N-acetyl-alpha-glucosaminidase may serve as a biomarker for Parkinson’s disease diagnosis,and that N-acetyl-alpha-glucosaminidase may reduceα-synuclein expression and 1-methyl-4-phenylpyridinium-induced n

关键词： biomarker diagnosis exosomes N-acetyl-alpha-glucosaminidase Parkinson’s disease proteomic α-synuclein

On quantitative structure-activity relationships between hydrazine derivatives and β irradiation

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Nuclear Science and Techniques 2018年第5期29卷 36-43页

作者： ling-Yu Wang Yan Wang Da-Qing cui Song-Tao xiao xiao-Dong Liu Ying-Gen OuYang Cong Huang Fundamental Science on Radioactive Geology and Exploration Technology Laboratory East China University of TechnologyNanchang 330013China China Institute of Atomic Energy Beijing 102413China 123

In this study, solutions of hydrazine and its derivatives were irradiated using a pulsed electron beam to determine the half-reaction time of radiolysis. 3 D structures of the hydrazine derivatives were optimized, and their energies were calculated using density functional theory with the B3 LYP method and 6-311 +(3 d, 3 p) basis *** the first time, the 3 D quantitative structure-activity relationship(QSAR) equation describing the relationship between the hydrazine derivative structures and rate of radiolysis has been established using SPSS *** correlation analysis revealed a close correlation between the total energies of the molecules and half-reaction times. In the QSAR equation, Y =-7583.464 +54.687 X_1+94333.586 X_2,Y,X_1,and X_2 are the half-reaction time, total energy of the molecule, and orbital transition energy, respectively. The significance levels of the regression coefficients were 0.006 and 0.031, i.e., both less than 0.05. Thus, this model fully explains the relationship between hydrazine derivatives and β radiolysis *** results show that the total energy of the molecule and orbital transition energy are the main factors that influence the β radiolysis stability of these hydrazine derivatives.

关键词： Hydrazine derivatives βIrradiation Radiolysis stability Quantitative structure–activity relationships

Dissecting the key genomic regions underlying high yield potential in common wheat variety‘Kenong 9204’

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Journal of Integrative Agriculture 2023年第9期22卷 2603-2616页

作者： ZHAO Chun-hua ZHANG Na FAN xiao-li JI Jun SHI xiao-li cui Fa ling Hong-qing LI Jun-ming College of Agriculture Ludong University/Key Laboratory of Molecular Module-based Breeding of High Yield and Abiotic Resistant Plants in Universities of ShandongYantai 264025P.R.China Jiangsu Xuhuai Regional Institute of Agricultural Sciences Xuzhou 221131P.R.China Chengdu Institute of Biology Chinese Academy of SciencesChengdu 610041P.R.China State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijng 100101P.R.China Center for Agricultural Resources Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesShijiazhuang 050022P.R.China Key Laboratory of Molecular and Cellular Biology Ministry of Education/Hebei Collaboration Innovation Center for Cell Signaling/Hebei Key Laboratory of Molecular and Cellular Biology/College of Life SciencesHebei Normal UniversityShijiazhuang 050024P.R.China

The foundation parents play key roles in the genetic improvement of both yield potential and end-use quality in *** the genetic basis that underlies certain beneficial traits in the foundation parents will provide theoretical reference for molecular breeding by a design approach.‘Kenong 9204’(KN9204)is a candidate foundation parent characterized by ideotype,high yield potential,and particularly high nitrogen fertilizer *** better understand the genetic basis of its high yield potential,high throughput whole-genome re-sequencing(10×)was performed on KN9204,its parental lines and its derivatives.A high-resolution genetic composition map of KN9204 was constructed,which showed the parental origin of the favorable genomic segments based on the identification of excellent yield-related quantitative trait loci(QTL)from a bi-parental mapping *** 693(XY693),a wheat–Thinopyrum ponticum partial amphidiploid,contributed a great deal to the high yield potential of KN9204,and three major stable QTLs from XY693 were fine *** transmissibility of key genomic segments from KN9204 to its derivatives were delineated,indicating that haplotype blocks containing beneficial gene combinations were conserved along with directional selection by *** for selection sweeps in the breeding programs was *** study provides a theoretical reference for the breeding of high-yield wheat varieties by a molecular design approach.

关键词： Kenong 9204 high-yielding potential quantitative trait locus genetic composition map key genomic regions