检索结果-南通市图书馆

Semaphorin 7A impairs barrier function in cultured human corneal epithelial cells in a manner dependent on nuclear factor-kappa B

引用

International Journal of Ophthalmology(English edition) 2024年第3期17卷 444-453页

作者： Cheng-Cheng Yang Xiu-Xia Yang xiao-jing zhao Heng Wang Zi-Han Guo Kai Jin Yang Liu Bin-Hui Li Department of Ophthalmology the Fifth Affiliated HospitalSun Yat-sen UniversityZhuhai 519000Guangdong ProvinceChina Department of Ophthalmology Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical UniversityHangzhou 310006Zhejiang ProvinceChina Eye Institute of Xiamen University Fujian Provincial Key Laboratory of Ophthalmology and Visual ScienceSchool of MedicineXiamen UniversityXiamen 361005Fujian ProvinceChina

●AIM:To evaluate the role of semaphorin 7A(Sema7A)and its associated regulatory mechanisms in modulating the barrier function of cultured human corneal epithelial cells(HCEs).●METHODS:Barrier models of HCEs were treated with recombinant human Sema7A at concentrations of 0,125,250,or 500 ng/mL for 24,48,or 72h in *** electrical resistance(TEER)as well as Dextran-fluorescein isothiocyanate(FITC)permeability assays were conducted to assess barrier *** quantify tight junctions(TJs)such as occludin and zonula occludens-1(ZO-1)at the mRNA level,reverse transcriptionpolymerase chain reaction(RT-PCR)analysis was *** was used to examine the activity of the nuclear factor-kappa B(NF-κB)signaling pathway and the production of TJs *** analyses were employed to localize the ***-linked immunosorbent assay(ELISA)and RT-PCR were utilized to observe changes in interleukin(IL)-1β*** investigate the role of NF-κB signaling activation and IL^(-1)βin Sema7A’s anti-barrier mechanism,we employed 0.1μmol/L IκB kinase 2(IKK2)inhibitor IV or 500 ng/mL IL^(-1)receptor(IL-1R)antagonist.●RESULTS:Treatment with Sema7A resulted in decreased TEER and increased permeability of Dextran-FITC in HCEs through down-regulating mRNA and protein levels of TJs in a time-and dose-dependent manner,as well as altering the localization of ***,Sema7A stimulated the activation of inhibitor of kappa B alpha(IκBα)and expression of IL-1β.The anti-barrier function of Sema7A was significantly suppressed by treatment with IKK2 inhibitor IV or IL-1R antagonists.●CONCLUSION:Sema7A disrupts barrier function through its influence on NF-κB-mediated expression of TJ proteins,as well as the expression of IL-1β.These findings suggest that Sema7A could be a potential therapeutic target for the diseases in corneal epithelium.

关键词： human corneal epithelial barrier function transepithelial electrical resistance zonula occludens-1 occludin nuclear factor-kappa B

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Mantle cell lymphoma with endobronchial involvement:A case report

引用

World Journal of Clinical Cases 2022年第8期10卷 2604-2609页

作者： Yi-Zong Ding Dao-Qiang Tang xiao-jing zhao Department of Thoracic Surgery Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Department of Pathology Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China

BACKGROUND Mantle cell lymphoma(MCL)is a subtype of Non-Hodgkin’s lymphoma(NHL).MCL frequently affects extranodal sites while endobronchial involvement is *** 5 cases of MCL with endobronchial involvement have been previously *** SUMMARY A 56-year-old male patient arrived at the hospital complaining of a dry cough.A mass in the right upper lobe of the lung was revealed in Chest computed tomography(CT).Right lung hilar and mediastinal lymphadenopathies were also found by CT *** patient was diagnosed with central-type lung cancer with multiple lymph node metastases after positron emission tomography(PET)CT scan *** fiber optic bronchoscope examination revealed diffuse neoplasm infiltration in the inlet of the right up lobar *** patient was finally diagnosed with MCL based on the bronchoscopy and mediastinoscopy biopsy *** MCL could masquerade as central type lung *** endobronchial biopsy examination is necessary for the early diagnosis of MCL.

关键词： Mantle cell lymphoma Endobronchial involvement Central type lung cancer Endobronchial biopsy Bronchoscopy Mediastinoscopy Case report

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The effects of surface ligands and counter cations on the stability of anionic thiolated M_(12)Ag_(32)(M=Au,Ag) nanoclusters

引用

Chinese Chemical Letters 2014年第6期25卷 839-843页

作者： Xin Zhang Hua-Yan Yang xiao-jing zhao Yu Wang Nan-Feng Zheng State Key Laboratory for Physical Chemistry of Solid Surfaces Xiamen University Department of Chemistry College of Chemistry and Chemical EngineeringXiamen University

The stabilities of [M12Ag32（SR）30]4- （M = Ag, Au and SR = SPhF2, SPhCF3, SPhF） clusters having the same structure but different surface ligands or counter cations were systematically studied. It was clearly revealed that a subtle structural change in the surface ligands or counter cations could significantly alter the overall stability of [M12Ag32（SR）30]4 although they all had an electronic structure of 18-electron superatom shell closure. SPhF2 was found as a better surface ligand than SPhCF3 or SPhF to stabilize [M12Ag32（SR）30]4-. And the use of more bulky [（PPhj）2N]＋ as the counter cations was revealed to be more deleterious to the overall stability of [M12Ag32（SR）30]4- clusters than PPh4＋. [Au12Ag32（SR）30]4- was much more stable than [Ag44（SR）30]4 with the same surface ligands and counter cations. An exceptional stability was observed on （PPh4）4[Au12Ag32（SPhF2）30] which was stable in DMF for more than 8 days in air at 80 ℃. More research efforts are still needed to deeply understand why a small structural change could result in a significant change in the stability of noble metal nanoclusters.

关键词： Nanoclusters Stability Ligand effect Noble metal

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Fecal microbial dysbiosis in Chinese patients with inflammatory bowel disease

引用

World Journal of Gastroenterology 2018年第13期24卷 1464-1477页

作者： Hai-Qin Ma Ting-Ting Yu xiao-jing zhao Yi Zhang Hong-Jie Zhang Department of Gastroenterology First Affiliated Hospital of Nanjing Medical University

AIM To analyze the alterations of fecal microbiota in Chinese patients with inflammatory bowel disease(IBD).METHODS Fecal samples from 15 patients with Crohn's disease(CD)(11 active CD, 4 inactive CD), 14 patients with active ulcerative colitis(UC) and 13 healthy individuals were collected and subjected to 16 S ribosomal DNA(rDNA) gene sequencing. The V4 hypervariable regions of 16 S rDNA gene were amplified from all samples and sequenced by the Illumina Mi Seq platform. Quality control and operational taxonomic units classification of reads were calculated with QIIME software. Alpha diversity and beta diversity were displayed with R *** Community richness(chao) and microbial structure in both CD and UC were significantly different from those in normal controls. At the phyla level, analysis of the microbial compositions revealed a significantly greater abundance of Proteobacteria in IBD as compared to that in controls. At the genera level, 8 genera in CD and 23 genera in UC(in particular, the Escherichia genus) showed significantly greater abundance as compared to that in normal controls. The relative abundance of Bacteroidetes in the active CD group was markedly lower than that in the inactive CD group. The abundance of Proteobacteria in patients with active CD was nominally higher than that in patients with inactive CD; however, the difference was not statistically significant after correction. Furthermore, the relative abundance of Bacteroidetes showed a negative correlation with the CD activity index *** Our study profiles specific characteristics and microbial dysbiosis in the gut of Chinese patients with IBD. Bacteroidetes may have a negative impact on inflammatory development.

关键词： Crohn’s disease Ulcerative colitis Chinese Microbial dysbiosis 16S ribosomal DNA

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Association of COL1A1 polymorphism with high myopia: a Meta-analysis

引用

International Journal of Ophthalmology(English edition) 2016年第4期9卷 604-609页

作者： Guang-Ming Jin xiao-jing zhao Ai-Ming Chen Yong-Xing Chen Qin Li State Key Laboratory of Ophthalmology Zhongshan Ophthalmic Center Sun Yat-sen University Department of Ophthalmology the Fifth Affiliated Hospital of Sun Yat-sen University Department of Pharmacy the Fifth Affiliated Hospital of Sun Yat-sen University Department of Otorhinolaryngology Jiangmen Central Hospital

AIM： To investigate the association between collagen type I alpha 1（COL1A1） gene and high myopia.· METHODS： In this Meta-analysis, we examined 5published case-control studies that involved 1942 high myopia cases and 2929 healthy controls to assess the association between the COL1A1 rs2075555 polymorphism and high myopia risk. We calculated the pooled odds ratios（ORs） of COL1A1 rs2075555 polymorphism in high myopia cases vs healthy controls to evaluate the strength of the association.·RESULTS： Overall, there was no significant difference both in the genotype and allele distributions of COL1A1rs2075555 polymorphism between high myopia cases and healthy controls： CC vs AA OR =1.10, 95% confidence interval（CI）=0.76-1.58; AC vs AA OR=0.98, 95%CI 0.80-1.20; CC/AC vs AA/OR=1.01, 95%CI 0.84-1.22; CC vs AC/AA OR =1.06, 95% CI =0.93-1.20; C vs A OR =1.06, 95% CI0.91-1.23）. In addition, in the stratified analyses by ethnicity, no significant associations were found in any genetic model both in European and Asia cohorts.·CONCLUSION： Our results indicate that the COL1A1rs2075555 polymorphism may not affect susceptibility to high myopia.

关键词： collagen type I alpha 1 polymorphism high myopia Meta-analysis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Tailoring Variant Pairing to Enhance Impact Toughness in High-Strength Low-Alloy Steels via Trace Carbon Addition

引用

Acta Metallurgica Sinica(English Letters) 2021年第6期34卷 755-764页

作者： Yi-Shuang Yu Zhi-Quan Wang Bin-Bin Wu jing-xiao zhao Xue-Lin Wang Hui Guo Cheng-Jia Shang Collaborative Innovation Center of Steel Technology University of Science and Technology BeijingBeijing 100083China Key Laboratory for Advanced Materials of Ministry of Education School of Materials Science and EngineeringTsinghua UniversityBeijing 100084China State Key Laboratory of Metal Material for Marine Equipment and Application Anshan 114021China

Alloying can make conventional metals reach ultra-high strength,but this usually comes at dramatic loss of *** this work,a desirable strength–toughness combination in high-strength low-alloy steel achieved via trace carbon *** significance of carbon in tailoring variant pairing and tuning impact toughness was elucidated from the perspective of crystallography and *** the carbon content increases,the packets and blocks are refined,and the-40 impact toughness *** enhancement of impact toughness results from the higher density of block boundaries,and the fracture mode shifts from brittle fracture to ductile–brittle combined fractures,then to ductile fracture due to the increased *** the carbon content would lower the martensite start temperature(M_S)temperature and driving force for martensitic transformation,and increase the strength of austenite matrix,which in turn contributes to producing more V1/V2 variant pairs to accommodate the transformation strain.

关键词： High-strength low-alloy steel Carbon Impact toughness Variant pairing Phase transformation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Tranilast inhibits TGF-β-induced collagen gel contraction mediated by human corneal fibroblasts

引用

International Journal of Ophthalmology(English edition) 2018年第8期11卷 1247-1252页

作者： Ye Liu xiao-jing zhao xiao-Shuo Zheng Hui Zheng Lei Liu Ling-Bin Meng Qin Li Yang Liu Department of Pathology the Fifth Affiliated HospitalSun Yat-sen University Department of Ophthalmology the Fifth Affiliated HospitalSun Yat-sen University Department of Ophthalmology the First Hospital of Jilin University Department of Internal Medicine Florida Hospital

AIM： To determine if tranilast affects human corneal fibroblast（HCFs） ***： HCFs cultured in a three-dimensional type I collagen gel were treated with or without transforming growth factor beta（TGF-β） or tranilast. Gel diameter was measured as an indicator for collagen contraction. Immunoblot was performed to evaluate myosin light chain（MLC） and paxillin phosphorylation. Confocal microscopy was employed to examine the focal adhesions and actin stress fiber formation. Immunoblot analysis and gelatin zymography were performed to detect tissue inhibitors of metalloproteinases and matrix metalloproteinases（MMPs） in ***： The inhibitory effect of tranilast on HCFsmediated collagen gel contraction induced by TGF-β was dose-dependent. The significant effect of tranilast was started from 100 μmol/L and maximized at 300 μmol/L. The peak effect of 300 μmol/L tranilast also relied on the duration of treatment, which showed statistical significance from day 2. TGF-β-induced paxillin and MLC phosphorylation, stress fiber formation, focal adhesions, and MMP-1, MMP-2, and MMP-3 secretion in HCFs were also inhibited by ***： Tranilast suppresses the HCFs-cultured collagen gel contraction induced by TGF-β. It attenuates actin stress fibers formation, focal adhesions, and the secretion of MMPs, with these actions likely contributing to the inhibitory effect on HCF contractility. By attenuating the contractility of corneal fibroblasts, tranilast treatment may inhibit corneal scarring.

关键词： tranilast transforming growth factor beta corneal fibroblast corneal wound healing

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Efficacy and economic benefits of a modified Valsalva maneuver in patients with paroxysmal supraventricular tachycardia

引用

World Journal of Clinical Cases 2020年第23期8卷 5999-6008页

作者： Wei Wang Teng-Fei Jiang Wei-Zhong Han Lin Jin xiao-jing zhao Ying Guo Internal Medicine-Cardiovascular Department Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250021Shandong ProvinceChina Department of Nursing The First Affiliated Hospital of Shandong First Medical University Department of Nursing Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250021Shandong ProvinceChina

BACKGROUND A modified Valsalva maneuver(VM)has been suggested to be superior to the standard VM for conversion of paroxysmal supraventricular tachycardia(PSVT).AIM To evaluate the efficacy and economic benefits of a modified VM in Chinese *** Patients with PSVT admitted to our center between October 2017 and September 2019 were randomly assigned to the modified and standard VM *** via VM was performed up to three *** primary outcome of the study was the success rate of PSVT conversion to sinus *** secondary outcomes included the incidence of adverse events,economic cost during the visit,and the degree of patient acceptance of the *** Overall,361 patients were enrolled,with 180 allocated to the modified VM group and 181 to the standard VM *** characteristics were well matched in the ***,the modified VM group had higher success rates of PSVT conversion after single(47.78%vs 15.38%,P0.05).Moreover,the economic cost of the clinic visit was significantly lower for the modified VM group than for the standard VM group(P<0.05).CONCLUSION The modified VM may confer both therapeutic and economic benefits as compared with the standard VM for conversion of PSVT.

关键词： Paroxysmal supraventricular tachycardia Modified Valsalva maneuver Costeffective analysis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Oxidative intramolecular coupling of 2,3-disubstituted phenyl acrylic acids and derivatives promoted by di-tert-butylperoxide

引用

Chinese Chemical Letters 2014年第2期25卷 348-350页

作者： De-Rong Ji Hua Yang xiao-jing zhao Hao Yang Yang-zhao Liu Dai-Hui Liao Chun Feng Cheng-Gang Zhang College of Chemistry and Materials Sichuan Normal University

Polymethoxy-substituted phenanthrene-9-carboxylic acids or their methylate are key intermediates for the synthesis of tylophora alkaloids and their analogs. An intramolecular oxidative coupling reaction of unfunctionalized 2,3-disubstituted phenyl acrylic acids and derivatives promoted by di-tert-butylper- oxide gave above intermediates in high yields. The mild reaction conditions and easy purification procedures of this method provide a new approach for the synthesis of phenanthrenes.

关键词： Polymethoxylphenanthrene Cross dehydrogenative coupling (cdc) Radical cation Intramolecular oxidative coupling

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

多重重组酶介导等温扩增技术检测食品中3种食源性致病菌

引用

食品安全质量检测学报 2024年第5期15卷 97-104页

作者：秦爱李根容邓方进张明娟周朝旭肖昭竞重庆市计量质量检测研究院重庆401121 中国电信股份有限公司重庆分公司重庆401121

目的建立快速、灵敏的多重重组酶介导等温扩增法(recombinase aided amplification, RAA)同时检测食品中的大肠埃希氏菌O157:H7、沙门氏菌和金黄色葡萄球菌。方法根据大肠埃希氏菌O157:H7的rfbE基因、沙门氏菌的invA基因以及金黄色葡... 详细信息

目的建立快速、灵敏的多重重组酶介导等温扩增法(recombinase aided amplification, RAA)同时检测食品中的大肠埃希氏菌O157:H7、沙门氏菌和金黄色葡萄球菌。方法根据大肠埃希氏菌O157:H7的rfbE基因、沙门氏菌的invA基因以及金黄色葡萄球菌的nuc基因的序列保守区域设计RAA特异性引物,筛选出最优的引物组合并优化各项实验条件;通过标准菌株验证方法特异性和灵敏度,并通过人工污染实验,验证方法在实际食品样本中的检测能力。结果多重RAA方法最佳扩增温度为37℃,反应时间为50min;方法特异性良好,仅对目标菌株存在特异性扩增条带,与其他常见食源性致病菌无交叉反应;方法对基因组DNA的检测灵敏度为0.10 ng/μL;对经过简单增菌的人工污染牛奶样本和牛肉干样本中目标菌株的检出限为100 CFU/mL。结论本研究建立的多重RAA扩增方法具有特异性好、灵敏度高、检测通量高等优势,适用于多种场景下食品样本中3种食源性致病菌的快速检测。

关键词：重组酶介导扩增技术大肠埃希氏菌O157:H7 沙门氏菌金黄色葡萄球菌

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：