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Melanization induced by Heliothis virescens ascovirus 3h promotes viral replication

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Insect Science 2021年第2期28卷 472-484页

作者： Zi-Qi Li xiao-hui song Min Wang Shu Wang Guo-Hua Huang Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests Hunan Agricultural UniversityChangshaChina College of Plant Protection Hunan Agricultural UniversityChangshaChina

Melanization is an important innate immune defense mechanism of insects,which can kill invading *** pathogens,for their survival and reproduction,inhibit the melanization of the ***,our results suggested that after infection with Heliothis virescens ascovirus 3h(HvAV-3h),the speed of melanization in infected Spodoptera exigiia larval hemolymph was accelerated and that the phenoloxidase(PO)activity of hemolymph in larvae infected with HvAV-3h increased significantly(1.20-fold at 96 hpi,1.52-fold at 120 hpi,1.23-fold at 144 hpi,1.12-fold at 168 hpi).The transcription level of the gene encoding *** prophenoloxidase-1(SePPO-1 gene)was upregulated dramatically in the fat body during the middle stage of *** addition,when melanization was inhibited or promoted,the replication of HvAV-3h was inhibited or promoted,*** conclusion,infection with HvAV-3h can markedly induce melanization in the middle stage of infection,and melanization is helpful for HvAV-3h viral replication.

关键词： Heliothis virescens ascovirus 3h melanization Spodoptera exigua viral replication

Decline of semen quality among Chinese sperm bank donors within 7 years （2008-2014）

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Asian Journal of Andrology 2017年第5期19卷 521-525页

作者： Li Wang Lin Zhang xiao-hui song Hao-Bo Zhang Cheng-Yan Xu Zi-Jiang Chen Center for Reproductive Medicine Shandong Provincial Hospital Affiliated to Shandong University Jinan China Shandong Provincial Key Laboratory of ReproductiveMedicine Jinan China National Research Center for Assisted Reproductive Technology and Reproductive Genetics Jinan China Key Laboratory for ReproductiveEndocrinology of Ministry of Education Jinan China

Semen from 5210 sperm bank donors was analyzed and trends in semen quality were evaluated at Shandong Human Sperm Bank between 2008 and 2014. After 2-7 days of abstinence, semen samples were collected. Measurements of semen volume, sperm concentration, sperm forward motility, and total sperm count were performed. There were significant declining trends in semen volume, sperm concentration, sperm forward motility, and total sperm count. Our results indicate that the quality of semen in this cohort of sperm donors had decreased during the study period.

关键词： male infertility semen quality sperm donation

Effect of SARS-CoV-2 infection on semen parameters in sperm bank volunteers with normal sperm concentration

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Asian Journal of Andrology 2024年第3期26卷 328-332页

作者： Li Wang Wen-Kai Han xiao-hui song Qi-Lin Zhang An-Quan Guan Yuan Gao Jin-Long Ma Center for Reproductive Medicine Shandong UniversityJinan 250001China State Key Laboratory of Reproductive Medicine and Offspring Health ShandongUniversityJinan 250001China Key Laboratory of Reproductive Endocrinology of Ministry of Education Shandong UniversityJinan 250001China Shandong KeyLaboratory of Reproductive Medicine Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250001China Shandong Provincial Clinical Research Center for Reproductive Health Jinan 250001China Shandong Technology Innovation Center for Reproductive Health Jinan 250001China NationalResearch Center for Assisted Reproductive Technology and Reproductive Genetics Shandong UniversityJinan 250001China Research Unit of Gametogenesis andHealth of ART-Offspring Chinese Academy of Medical Sciences(No.2021RU001)Jinan 250001China

In this study,we aimed to assess the effect of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection on *** study comprised 110 sperm volunteers who self-reported SARS-CoV-2 infection from the Human Sperm Bank ofthe Center for Reproductive Medicine,Shandong University(Jinan,China).The volunteers had normal sperm concentration *** volunteer provided semen samples before and after *** selected 90 days after infection as the cutoff *** parameters within 90 days after infection of 109 volunteers(group A)were compared with semen parameters before ***,semen parameters on or after 90 days after infection of 36 volunteers(group B)were compared with semen parameters ***,based on whether the volunteers had completed the three-dose SARS-CoV-2 vaccination booster,volunteersin group A and B were further divided into two subgroups *** parameters were compared before and after infection ineach *** results showed that in this cohort population,the semen quality in volunteers with normal sperm concentrationsbefore infection decreased after SARS-CoV-2 infection within 90 days,while the semen quality returned to preinfection levels after *** completion of a three-dose SARS-CoV-2 vaccination booster may exert a protective effect on semen quality after infection.

关键词： SARS-CoV-2 SARS-CoV-2 vaccination semen quality sperm donation sperm parameters

Comparative transcriptomic analysis reveals adriamycin-induced apoptosis via p53 signaling pathway in retinal pigment epithelial cells

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2018年第12期19卷 895-909页

作者： Yu-chen LIN Ze-ren SHEN xiao-hui song Xin LIU Ke YAO Eye Center the Second Affiliated HospitalZhejiang University School of MedicineHangzhou 310009China Key Laboratory of Ophthalmology of Zhejiang Province Hangzhou 310009China

Objective: This paper applied a transcriptomic approach to investigate the mechanisms of adriamycin(ADR) in treating proliferative vitreoretinopathy(PVR) using ARPE-19 cells. Methods: The growth inhibitory effects of ADR on ARPE-19 cells were assessed by sulforhodamine B(SRB) assay and propidium iodide(PI) staining using flow cytometry. The differentially expressed genes between ADR-treated ARPE-19 cells and normal ARPE-19 cells and the signaling pathways involved were investigated by microarray analysis. Mitochondrial function was detected by JC-1 staining using flow cytometry and the Bcl-2/Bax protein family. The phosphorylated histone H2 AX(γ-H2 AX), phosphorylated checkpoint kinase 1(p-CHK1), and phosphorylated checkpoint kinase 2(p-CHK2) were assessed to detect DNA damage and repair. Results: ADR could significantly inhibit ARPE-19 cell proliferation and induce caspasedependent apoptosis in vitro. In total, 4479 differentially expressed genes were found, and gene ontology items and the p53 signaling pathway were enriched. A protein–protein interaction analysis indicated that the TP53 protein molecules regulated by ADR were related to DNA damage and oxidative stress. ADR reduced mitochondrial membrane potential and the Bcl-2/Bax ratio. p53-knockdown restored the activation of c-caspase-3 activity induced by ADR by regulating Bax expression, and it inhibited ADR-induced ARPE-19 cell apoptosis. Finally, the levels of the γ-H2 AX, p-CHK1, and p-CHK2 proteins were up-regulated after ADR exposure. Conclusions: The mechanism of ARPE-19 cell death induced by ADR may be caspase-dependent apoptosis, and it may be regulated by the p53-dependent mitochondrial dysfunction, activating the p53 signaling pathway through DNA damage.

关键词： Adriamycin Proliferative vitreoretinopathy Retinal pigment epithelial p53 Apoptosis

Ammonia Volatilization Losses from Urea Applied to Wheat on a Paddy Soil in Taihu Region, China

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Pedosphere 2005年第1期15卷 59-65页

作者： FANxiao-hui songYong-Sheng LINDe-Xi YANGLin-Zhang ZHOUJian-Min InstituteofSoilScience ChineseAcademyofSciencesNanjing210008China

Ammonia volatilization losses from urea applied as a basal fertilizer and a top dressing at tillering stage in a wheat field of Taihu Region, China, were measured with a micrometeorological technique. Urea as fertilizer was surface broadcast at 81 (low N) and 135 (high N) kg N ha-1 as basal at the 3-leaf stage of the wheat seedling on December 2002, and 54 (low N) and 90 (high N) kg N ha-1 as top dressing on February 2003. Ammonia volatilization losses occurred mainly in the first week after applying N fert…

关键词： ammonia volatilization urea wheat

Electrochemical conversion of methane to bridge the gap in the artificial carbon cycle

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Journal of Energy Chemistry 2025年第1期100卷 286-308页

作者： Yuhao Peng Yuefeng song Ihar Razanau Juanxiu xiao Wei xiao Di Hu Guoxiong Wang College of Chemistry and Molecular Sciences Hubei Key Laboratory of Electrochemical Power Sources Wuhan University State Key Laboratory of Catalysis Dalian National Laboratory for Clean Energy iChEM (Collaborative Innovation Center of Chemistry for Energy Materials) Dalian Institute of Chemical Physics Chinese Academy of Sciences Laboratory of Physical-Chemical Technologies SSPA Scientific and Practical Materials Research Centre of NAS of Belarus State Key Laboratory of Marine Resources Utilization in South China Sea Collaborative Innovation Center of Marine Science and Technology School of Marine Science and Engineering Hainan University Department of Chemical and Environmental Engineering University of Nottingham Ningbo China

Methane, an abundant one-carbon（C1） resource, is extensively used in the industrial production of vital fuels and value-added chemicals. However, current industrial methane conversion technologies are energy-and carbon-intensive, mainly due to the high activation energy required to break the inert C–H bond, low selectivity, and problematic side reactions, including CO2emissions and coke deposition. Electrochemical conversion of methane（ECM） using intermittent renewable energy offers an attractive solution, due to its modular reactor design and operational flexibility across a broad spectrum of temperatures and pressures. This review emphasizes conversion pathways of methane in various reaction systems, highlighting the significance and advantages of ECM in facilitating a sustainable artificial carbon cycle. This work provides a comprehensive overview of conventional methane activation mechanisms and delineates the complete pathways of methane conversion in electrolysis contexts. Based on surface/interface chemistry, this work systematically analyzes proposed reaction pathways and corresponding strategies to enhance ECM efficiency towards various target products, including syngas, hydrocarbons, oxygenates, and advanced carbon materials. The discussion also encompasses opportunities and challenges for the ECM process, including insights into ECM pathways, rational electrocatalyst design, establishment of benchmarking protocols, electrolyte engineering, enhancement of CH4conversion rates, and minimization of CO2emission.

关键词： Methane Electrochemical conversion Reaction mechanism Catalyst design Electrode Electrocatalysis

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基于辅助阵元的近场源幅相误差校正算法

电子与信息学报 2018年第10期40卷 2415-2422页

作者：倪萌钰陈辉校松倪柳柳张佳佳空军预警学院雷达兵器运用工程重点实验室武汉430019

针对近场源的定位及阵列幅相误差校正问题,该文提出一种基于均匀对称阵列利用辅助阵元矢量重构解耦合的幅相误差校正方法。通过重构虚拟阵列实现距离参数的分离,再通过对虚拟阵列导向矢量的变换实现方位和幅相误差之间的解耦合;最后通... 详细信息

针对近场源的定位及阵列幅相误差校正问题,该文提出一种基于均匀对称阵列利用辅助阵元矢量重构解耦合的幅相误差校正方法。通过重构虚拟阵列实现距离参数的分离,再通过对虚拟阵列导向矢量的变换实现方位和幅相误差之间的解耦合;最后通过对实阵列导向矢量的变换,实现距离与幅相误差的解耦合,从而实现对近场源的方位角、距离以及阵列的幅相误差系数的级联估计。仿真结果表明所提算法相比现有算法运算量小,方位及距离参数估计精确,幅相误差校正精度高。

关键词：对称阵列近场源辅助阵元矢量重构幅相误差校正

Ultrasonic Associated Synthesis of Pentacyclo[5.4.0.02,6.03,10.05,9]undecane-8,11-diol

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Ultrasonic Associated Synthesis of Pentacyclo[5.4.0.02,6.03,...

Synergistically enhanced ORR and HER performance on Co-N-C coupled in-situ generated PtCo intermetallic

中国化学会第26届学术年会有机化学分会场

作者： xiao-hui song, Yun-Zhou Xue, Hong Yan College of Life Science and Bioengineering Beijing University of Technology, Beijing 100124

＜正＞Polycyclic cages have held a special fascination for organic chemists for their considerable strain energy as evidence by the fact that they contain unusually long framework C-C σ-bond, C-C-C bond angles that d...

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Journal of Energy Chemistry 2025年第1期100卷 721-729页

作者： Guanyu Luo Min song Lulu An xiao Huang Qian Zhang Chenhao Zhang Tao Shen Shuang Wang Deli Wang Key Laboratory of Material Chemistry for Energy Conversion and Storage (Huazhong University of Science and Technology) Ministry of Education School of Chemistry and Chemical Engineering Huazhong University of Science and Technology

Integrating multi-scale sites in a composite catalyst is vital to realize efficient ***,a synergistic composite catalyst consisting of Co atomic sites and in-situ generated PtCo intermetallic compounds （IMCs）(o-PtCo@CoNC) is proposed through Co pre-anchoring and subsequent impregnation-reduction *** loading of Co atoms provides a chance for in-situ generating PtCo ordered intermetallic *** remaining Co single atoms and PtCo IMCs construct synergistic electrocatalytic *** from the ordered structure,synergistic effect of Pt Co IMCs and Co single atoms,o-PtCo@CoNC exhibits excellent electrocatalytic performance for oxygen reduction reaction （ORR） and hydrogen evolution reaction （HER） with mass activity of 1.21 A mgPt-1（at 0.9 V） and5.70 A mgPt-1（at an overpotential of 100 mV）,***,o-PtCo@CoNC delivers negligible loss of half-wave potential and overpotential during long-term stability test in acid solutions,with 13 mV decay after 50,000 potential cycles for ORR and a 2.7 mV decay after 20,000 potential cycles for *** integration strategy of single-atomic sites coupled IMCs paves the way for enhancing the activity and durability of Pt-based electrocatalysts.

关键词： Electrolytic reduction