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Heterogeneity in predisposition of hepatic cells to be induced into pancreatic endocrine cells by PDX-1

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World Journal of Gastroenterology 2005年第15期11卷 2277-2282页

作者： ShunLu Wei-PingWang xiao-feiwang Zong-MeiZheng PingChen Kang-TaoMa Chun-YanZhou DepartmentofBiochemistryandMolecularBiology SchoolofBasicMedicalSciencesPekingUniversityBeijing100083China PekingUniversityStemCellResearchCenter Beijing100083China

AIM: The role of Pancreatic and Duodenal Homeobox-1(PDX-1) as a major regulator of pancreatic development determines the function and phenotype of β cell. In this study, potential plasticity of liver cells into pancreatic endocrine cells induced by PDX-1 was ***: Human hepatoma cell line HepG2 was stably transfected with mammalian expression plasmid pcDNA3-PDX encoding human PDX-1 gene. Ectopic expression of PDX-1 and insulin were detected by RT-PCR,Western blot and/or immunostaining. PDX-1+ HepG2 cells were transplanted under renal capsule of STZ-induced diabetic nude mice (n = 16) to examine the inducing effect in ***: Exogenous PDX-1 transgene was proved to express effectively in HepG2 cell at both mRNA and protein levels. The expression of endogenous insulin and some βcell-specific differentiation markers and transcription factors were not induced in PDX-1+ HepG2 cells. When transplanted under renal capsule of STZ-induced diabetic nude mice, PDX-1+ HepG2 cells did not generate insulinproducing cells. These data indicated that stable transfected PDX-1 could not convert hepatoma cell line HepG2 to pancreatic cells in vitro or in vivo. Mature hepatocytes might need much more complicated or rigorous conditions to be shifted to insulin-producing ***: The expression of exogenous PDX-1 is not sufficient to induce relatively mature hepatocytes differentiating into insulin-producing cells.

关键词： PDX-1 HepG2, Insulin Transgene Diabetes

Enhanced Resistance of Snowdrop Lectin （Galanthus nivalis L. agglutinin） Expressing Maize to Asian Corn Borer （Ostriniafurnacalis Guenée）

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Journal of Integrative Plant Biology 2005年第7期47卷 873-880页

作者： Zhao-YuWANG xiao-FenSUN feiwang Ke-XuanTANG Ju-RenZHANG SchoolofLifeSciences ShandongUniversityJinan250100China StateKeyLaboratoryofGeneticEngineering InstituteofGeneticsSchoolofLifeSciencesFudanUniversityShanghai200433China SchoolofAgricultureandBiology ShanghaiJiaoTongUniversityShanghai200030China

In order to enhance the resistance to pests, transgenic maize (Zea mays L.) plants from elite inbred lines containing the gene encoding snowdrop lectin (Galanthus nivalis L. agglutinin; GNA) under control of a phloem-specific promoter were generated through the Agrobacterium tumefaciens-mediated also studied. Thirty-six independently derived plants were subjected to molecular analyses. The level of GNA expression at 0.13%-0.28% of total soluble protein was observed in different transgenic plants. The progeny of three GNA-expressing independent transformants that were derived separately from the elite inbred lines DH4866, DH9942, and 8902, were selected for examination of resistance to ACB. These plants synthesized GNA at levels above 0.24% total soluble protein and enhanced resistance to ACB was demonstrated by exposing the plants to insects under greenhouse conditions. Semi-artificial diet bioassays also showed the toxic effect of GNA on ACB. Field evaluation of the transgenic plants supported the results from the artificial trial. In the present study, we have obtained new insect-resistant maize material for further breeding work and have found that GNA-expressing plants not only gained significant resistance to homopterans, but also showed toxicity to ACB, which is a type of Lepidoptera.

关键词： com borer Galanthus nivalis agglutinin gene(gna) maize resistance to pests transgene.

Review on COVID-19 and acute lung injury

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Journal of Hainan Medical University 2020年第12期26卷 10-14页

作者： Shuang-Qin Xu Jin Qian Qi-Feng Huang Tang Deng Li-Hua Li Qi Li Hang-feiwang Zhi-Qian Luo xiao-Ran Liu Chuan-Zhu Lv Hainan Medical College No.3 Xueyuan RoadLonghua DistrictHaikou CityHainan 571199 Emergency Department of the first affiliated Hospital of Hainan Medical College No.31 Longhua RoadLonghua DistrictHaikou CityHainan 570102 Key Laboratory of Emergency and Trauma Research of Ministry of Education Hainan Medical UniversityHaikouHainan 571199China Research Unit of Island Emergency Medicine Chinese Academy of Medical Sciences(No.2019RU013)Hainan Medical UniversityHaikouHainan 571199China

The epidemic caused by the new coronavirus infection is a global public health emergency. The new coronavirus pneumonia (COVID-19) has caused more deaths in China than the SARS-CoV outbreak in 2003. Since the virus has a high homology with SARS-CoV, it is speculates that it may infect cells through the S protein-ACE2 pathway then cause tracheal epithelial cell damage and alveolar damage, further induce inflammatory factor storm and trigger apoptosis cascade reaction and other mechanisms leading to acute lung injury. At early stage, the new coronavirus pneumonia mainly presents with non-specific manifestations of fatigue, fever, cough, etc., Then the respiratory tract will be involved, and evolved into the new type of infectious disease with lung lesions. Under radiology images, ground glass-like changes can be observed in the lungs. With its aggravation, progressive hypoxemia, acute respiratory distress syndrome, and even death occurs. At present, the correlation between the new coronavirus pneumonia and acute lung injury is not yet clear. This article reviews the characteristics of the new coronavirus, the possible mechanism of acute lung injury in new coronavirus pneumonia, post-infection diagnosis and current treatment options in order to provide evidence for its treatment.

关键词： New coronavirus Acute lung injury(ALI) Acute respiratory distress syndrome (ARDS) Treatme

咔唑基团功能化全同聚丙烯抗紫外老化性能研究

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高分子学报 2022年第12期53卷 1523-1533页

作者：马松峰刘超孙晓伟高欢王菲潘莉李悦生天津大学材料科学与工程学院天津300350

通过丙烯和含咔唑基团的α-烯烃的直接共聚,向全同聚丙烯中引入了具有可吸收紫外光的咔唑基团.咔唑基团具有吸收紫外光及365 nm紫外光下具有明显的蓝紫色发光特性,可能赋予材料抗光氧老化性能.利用核磁共振波谱和红外等对丙烯/11-咔唑-1... 详细信息

通过丙烯和含咔唑基团的α-烯烃的直接共聚,向全同聚丙烯中引入了具有可吸收紫外光的咔唑基团.咔唑基团具有吸收紫外光及365 nm紫外光下具有明显的蓝紫色发光特性,可能赋予材料抗光氧老化性能.利用核磁共振波谱和红外等对丙烯/11-咔唑-1-十一碳烯(P-C)共聚物结构进行了明确表征,共聚物中咔唑基团的含量分别为0.45 mol%~1.67 mol%.对不同咔唑基团含量的P-C样品进行了紫外老化处理,并对其抗紫外老化性能进行了研究.在相同老化条件下,当丙烯均聚物(PP)样品表面出现大而深的裂纹时,P-C样品表面没有裂纹或仅出现了细微的裂纹.与iPP样品相比,老化后的P-C样品拉伸性能、热性能等在老化过程中得到了良好保持,降解反应受到明显抑制.综上所述,含有共轭结构的咔唑基团的引入能有效增强聚丙烯的抗紫外老化性能.

关键词：全同聚丙烯咔唑基团配位聚合抗紫外老化

The role and mechanism of midkine in malignant tumors and inflammation

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Journal of Hainan Medical University 2020年第14期26卷 63-66页

作者： Qi-Feng Huang Tang Deng Li-Hua Li Jin Qian Qi Li Shuang-Qin Xu Hang-feiwang xiao-Ran Liu Emergency Department the First Affiliated Hospital of Hainan Medical universityHaikouHainan 570102China Hainan Medical University HaikouHainan 570000China

Midkine(MK)is a heparin-bound growth *** MK was discovered and only expressed during the embryonic *** the advances of research,MK was found to be expressed in many diseases,such as tumor diseases(pancreatic cancer,liver cancer,gastric cancer,lung cancer,etc.),acute orchronic inflammatory diseases(ARDS,Chronic renal failure,COPD,etc.),which are related to the molecular structure,signaling pathways and biological effects of *** the signaling pathway mechanism of MK has not been fully elucidated,it is necessary to study its signaling pathway mechanism and play a key role in the treatment of some clinical *** article summarizes the molecular structure of MK and related mechanisms of MK and related diseases,and summarizes how MK participates in the occurrence and development of *** provide a reference for MK may become an important target for disease diagnosis and treatment.

关键词： Midkine(MK) Signaling pathway Biological effect