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Blocking NF-kB nuclear translocation leads to p53-related autophagy activation and cell apoptosis

World Journal of Gastroenterology 2011年第4期17卷 478-487页

作者： Bao-Song Zhu Chun-Gen Xing fang Lin xiao-qing fan Kui Zhao Zheng-Hong Qin Department ofGeneral Surgery The Second Affiliated Hospital Soochow University Suzhou 215006 Jiangsu Province China Laboratory of Aging and Nervous Diseases Soochow University School of Medicine Suzhou215123 Jiangsu Province China Department of General Surgery The Second Hospital Of Jiaxing City Jiaxing 314000 Jiangsu ProvinceChina

AIM: To investigate the anti-tumor effects of nuclear factor-κB (NF-κB) inhibitor SN50 and related mechanisms of SGC7901 human gastric carcinoma cells. METHODS: MTT assay was used to determine the cytotoxic effects of SN50 in gastric cancer cell line SGC7901. Hoechst 33258 staining was used to detect apoptosis morphological changes after SN50 treatment. Activation of autophagy was monitored with monodansylcadaverine (MDC) staining after SN50 *** staining was used to detect the expression of light chain 3 (LC3). Mitochondrial membrane potential was measured using the fluorescent probe JC-1. Western blotting analysis were used to determine the expression of proteins involved in apoptosis and autophagy including p53, p53 upregulated modulator of apoptosis (PUMA), damage-regulated autophagy modulator (DRAM), LC3 and Beclin 1. We detected the effects of p53-mediated autophagy activation on the apoptosis of SGC7901 cells with the p53 inhibitor pifithrin-α. RESULTS: The viability of SGC7901 cells was inhibited after SN50 treatment. Inductions in the expression of apoptotic protein p53 and PUMA as well as autophagic protein DRAM, LC3 and Beclin 1 were detected with Western blotting analysis. SN50-treated cells exhibited punctuate microtubule-associated protein 1 LC3 in immunoreactivity and MDC-labeled vesicles increased after treatment of SN50 by MDC staining. Collapse of mitochondrial membrane potential Δψ were detected for 6 to 24 h after SN50 treatment. SN50-induced increases in PUMA, DRAM, LC3 and Beclin 1 and cell death were blocked by the p53 specific inhibitor pifithrin-α. CONCLUSION: The anti-tumor activity of NF-κB inhibitors is associated with p53-mediated activation of autophagy.

关键词： Nuclear factor-κB SN50 Autophagy P53 Cell apoptosis

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Effects of LY294002 on the invasiveness of human gastric cancer in vivo in nude mice

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World Journal of Gastroenterology 2009年第40期15卷 5044-5052页

作者： Chun-Gen Xing Bao-Song Zhu xiao-qing fan Hui-Hui Liu Xun Hou Kui Zhao Zheng-Hong Qin Department of General Surgery The Second Affiliated Hospital Soochow University Suzhou 215006 Jiangsu Province China Department of General Surgery The Second Hospital of Jiaxing City Jiaxing 314000 Jiangsu Province China Department of Neurology The Second Affiliated Hospital Soochow University Suzhou 215006 Jiangsu Province China Laboratory of Aging and Nervous Diseases Soochow University School of Medicine Suzhou 215123 Jiangsu Province China

AIM: To investigate the effects of class phosphatidy-linositol 3-kinase (PI3K) inhibitor LY294002 on the invasiveness and related mechanisms of implanted tumors of SGC7901 human gastric carcinoma cells in nude mice. METHODS: Nude mice were randomly divided into model control groups and LY294002 treatment groups. On days 5, 10 and 15 after treatment, the inhibitory rate of tumor growth, pathological changes in tumor specimens, expression levels of matrix metalloproteinase (MMP)-2, MMP-9, CD34 [representing microvessel density (MVD)] and vascular endothelial growth factor (VEGF), as well as apoptosis indexes in tumor samples were ***: In this study, we showed that treatingthe tumors with LY294002 could significantly inhibit carcinoma growth by 11.3%, 29.4% and 36.7%, after 5, 10 and 15 d, respectively, compared to the control group. Hematoxylin & eosin staining indicated that the rate of inhibition increased progressively (23.51% ± 3.11%, 43.20% ± 3.27% and 63.28% ± 2.10% at 5, 10 and 15 d, respectively) along with apoptosis. The expression of MMP-2 was also downregulated (from 71.4% ± 1.6% to 47.9% ± 0.7%, 31.9% ± 0.9% and 7.9% ± 0.7%). The same effects were observed in MMP-9 protein expression (from 49.4% ± 1.5% to 36.9% ± 0.4%, 23.5% ± 0.9% and 7.7% ± 0.6%), the mean MVD (from 51.2% ± 3.1% to 41.9% ± 1.5%, 30.9% ± 1.7% and 14.9% ± 0.8%), and the expression of VEGF (from 47.2% ± 3.1% to 25.9% ± 0.5%, 18.6% ± 1.2% and 5.1% ± 0.9%) by immunohistochemical ***: The classPI3K inhibitor LY294002could inhibit the invasiveness of gastric cancer cells by downregulating the expression of MMP-2, MMP-9, and VEGF, and reducing MVD.

关键词： Phosphatidylinositol 3-kinase LY294002 Gastric cancer Neoplasm invasiveness

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Chemoradiotherapy followed by surgery could improve the efficacy of treatments in patients with resectable esophageal carcinoma

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Chinese Medical Journal 2013年第16期126卷 3138-3145页

作者： WANG Feng WANG Ya-mei HE Wei LI Xiang-ke PENG fang-hui YANG xiao-li fan qing-xia Department of Oncology First Affiliated Hospital of ZhengzhouUniversity Zhengzhou Henan 450052 China

Background The effectiveness of chemoradiotherapy followed by surgery （CRTS） in patients with resectable esophageal carcinoma remains *** performed a systematic review of the literature with *** Electronic databases were used to identify published studies between January 1992 and April *** relative risk （RR） with 95％ confidence interval （95％ CI） was utilized to estimate the strength of the association between CRTS and surgery alone （SA） survival of the resectable esophageal carcinoma *** and publication bias were also assessed in the present *** The final analysis of 2755 resectable esophageal carcinoma cases from 21 randomized controlled trials （RCTs） are *** to the SA group,the 1,3-and 5-year survival rates were significantly higher in the CRTS group （all P ＜0.05）; the 3-and 5-year survival rates for the Eastern patients,Western patients,patients undergoing concurrent chemoradiotherapy,patients with squamous cell carcinoma,patients undergoing High-dose radiotherapy （≥40 Gy）,and patients given either ＂cisplatin ＋ Fluorouracil＂ or ＂cisplatin ＋ paclitaxel＂ chemotherapy were significantly higher in the CRTS group （all P ＜0.05）.There were no statistical significances in the 3-and 5-year survival rates for patients undergoing sequential chemoradiotherapy or patients with adenocarcinoma between the two groups （all P ＞0.05）.Compared to the RCTS group,the surgery rate in the SA group was higher （P ＜0.05）,while the CRTS group had significantly higher radical resection rate,R0 resection rate and lower postoperative local recurrence rate （all P ＜0.05）.The differences in postoperative complication incidence,post-operative distant metastasis and postoperative mortality rate were not statistically significant between the two groups （all P ＞0.05）.Conclusion CRTS can significantly improve the survival and surgical conditions of patients with resectable esophageal carcinoma.

关键词： oesophageal carcinoma chemoradiotherapy followed by surgery surgery alone randomized controlled trial meta-analysis

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非二进制SAR ADC的电容失配校正方法

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计算机工程与设计 2018年第6期39卷 1603-1609页

作者：陈晓青叶凡复旦大学专用集成电路与系统国家重点实验室上海201203

研究13比特逐次逼近型模数转换器的电容失配问题,提出结合DEM技术的基于LMS算法的校正方法。分析电容失配对权重的影响,为减小高精度ADC的面积开销,采用冗余结构的分段电容阵列,降低对电容失配的要求,为校正提供条件,设计基于LMS算法的... 详细信息

研究13比特逐次逼近型模数转换器的电容失配问题,提出结合DEM技术的基于LMS算法的校正方法。分析电容失配对权重的影响,为减小高精度ADC的面积开销,采用冗余结构的分段电容阵列,降低对电容失配的要求,为校正提供条件,设计基于LMS算法的结合DEM技术的校正方法。在MATLAB中搭建模型进行仿真,仿真结果表明,采用校正方法后INL可以达到-1.36/1.26LSB。

关键词：模数转换器逐次逼近最小均方算法动态元件匹配伪动态权重数模转换器

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Integrating lipidomics and gut microbiota to study the anti-hyperlipidemia effect of Sargentodoxae Caulis extract

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Traditional Medicine Research 2025年第1期10卷 1-11页

作者： xiao-xiao Zhang Li Jia Xiu-Jun Zhan Charity Ngina qing-Rui Zhang xiao-Ge Li Qi-Bao Jiang Shi Bai Miao-Miao Jiang National Key Laboratory of Chinese Medicine Modernization Tianjin University of Traditional Chinese MedicineTianjin 301617China Haihe Laboratory of Modern Chinese Medicine Tianjin 301617China School of Medicine Taizhou UniversityTaizhou 318000China.

Background:Sargentodoxae Caulis(SC)is the vine stem of Sargentodoxa Cuneata(Oliv.)Rehd.&*** in ***,and it in traditional Chinese medicine has been known for promoting blood circulation and removing blood stasis as recorded in the ancient book“Illustrated Classics of Materia Medica”.It has been used effectively to treat blood stasis too in modern clinical ***,the anti-hyperlipidemia effect of SC is not fully *** paper aims at exploring the use of SC stems to improve the balance of blood lipids in the body,and its new role in treating ***:The effects of SC extract on hyperlipidemia were explored by combining lipidomics and gut ***,we explored the potential mechanism of SC in treating hyperlipidemia by pathway ***:The results showed that the stem extract of SC could restore the physiological and biochemical indices of hyperlipidemia in mice,as well as repair the morphological and structural damage to *** to the Model group,the SC extract significantly reduced the liver index,epididymal fat index,and Lee’s *** also significantly decreased serum levels of total cholesterol,triglycerides,low-density lipoprotein cholesterol,peroxisome proliferator-activated receptor gamma(PPAR-γ),D-lactate,and free fatty acids,while significantly increasing the relative content of peroxisome proliferator-activated receptor alpha(PPAR-α).These changes were statistically ***-targeted lipidomics,based on LC-MS,were utilized to investigate the lipid metabolism characteristics in serum,liver,and epididymal fat of the *** was observed that,compared to the blank group,the Model group exhibited significant changes primarily in glycerol lipids and *** treatment group also displayed alterations in these lipids.A total of 38,81,and 27 differential lipids were identified in serum,liver,and epididymal fat samples,*** these,14 common differenti

关键词： hyperlipidemia Sargentodoxae Caulis lipidomics intestinal flora

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De novo synthesis of atropisomeric benzofurans via Cu/SPDO complex catalyzed asymmetric formal [3 + 2] annulation

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Science China Chemistry 2025年第1期 174-180页

作者： fan-xiao Meng Xing-Yu Wang Ka Lu Ju-Song Yang Fu-Min Zhang Ai-Jun Ma Bao Du Yong-Qiang Tu State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical Engineering Lanzhou University School of Chemistry and Chemical Engineering Shanghai Jiao Tong University School of Biotechnology and Health Sciences Wuyi University

Herein, the de novo synthesis of atropisomeric benzofurans through the Cu/spirocyclic-pyrrolidine-oxazoline(SPDO) complex catalyze asymmetric formal [3 + 2] cycloaddition between quinones and ynamides is explored, producing a range of structurally diverse axially chiral benzofurans in satisfactory yields with good to excellent enantioselectivities. Furthermore, density functional theory calculations were applied to explain the possible reaction mechanism.

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P185^(c-erBb-2)胞外区结构域在大肠杆菌BL21中的表达分析

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吉林大学自然科学学报 2001年第3期 76-78页

作者：王颖曾庆银何晓红孟庆繁高波傅学奇吉林大学生命科学学院药学系长春130023

以 PET-HT为表达载体 ,探讨在大肠杆菌 BL2 1中高效表达 P1 85c- er Bb- 2胞外区结构域蛋白的最佳条件 .经 SDS-PAGE与软件分析 ,确定其最佳表达时间为 3 h,当菌液密度为 0 .7时 ,所表达的蛋白含量最高 ,而 IPTG在一定浓度范围内对表达... 详细信息

以 PET-HT为表达载体 ,探讨在大肠杆菌 BL2 1中高效表达 P1 85c- er Bb- 2胞外区结构域蛋白的最佳条件 .经 SDS-PAGE与软件分析 ,确定其最佳表达时间为 3 h,当菌液密度为 0 .7时 ,所表达的蛋白含量最高 ,而 IPTG在一定浓度范围内对表达含量不产生影响 .

关键词： P185c-erBb-2 SDS-聚丙烯酰胺凝胶电泳表达大肠杆菌 BL21 跨膜糖蛋白胞外区结构域

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Integration of perioperative features and intragraft TCF7L2 expression to predict lipid metabolic disorder in liver transplant recipients

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Science China Life Sciences 2025年第1期 297-299页

作者： Junbin Zhou Rongli Wei Guanghan fan Zhengxing Lian Xuanyu Zhang Linsong Tang Zhetuo Qi Haiyang Xie Shusen Zheng Qiang Wei xiao Xu Department of Hepatobiliary and Pancreatic Surgery People's Hospital of LishuiAffiliated Lishui Hospital of Wenzhou Medical UniversityThe First Affiliated Hospital of Lishui University NHFPC Key Laboratory of Combined Multi-Organ Transplantation Key Laboratory of Integrated Oncology and Intelligent Medicine of Zhejiang Province Department of Hepatobiliary and Pancreatic SurgeryHangzhou First People's Hospital Department of Hepatobiliary and Pancreatic Surgery The First Affiliated HospitalZhejiang University School of Medicine Department of Urology The Second Affiliated HospitalZhejiang University School of Medicine Department of Hepatobiliary and Pancreatic Surgery Department of Liver TransplantationShulan (Hangzhou) Hospital Hangzhou Medical College Zhejiang University School of Medicine

Dear Editor, Liver transplantation(LT) is the only curative therapy for patients with endstage liver disease(He et al., 2023; Ling et al., 2022). Post-transplant hypertriglyceridemia(PTHT), which has an incidence of up to 32.8% in 6 months after LT, is one of the major post-transplant complications(Toshima et al., 2020).

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一类生物演化模型的统计特性

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系统工程理论与实践 2003年第1期23卷 77-81页

作者：龚小庆范文涛杭州商学院统计与计算科学学院浙江杭州310012 中国科学院武汉物理与数学研究所湖北武汉430071

在给出的生态系列演化模型中 ,首先 ,每次只让适应度最小的物种发生变异 ,直到其适应度比原来高为止 ,对此我们给出了每一次变异所需时间的数学期望 ,并证明了最小适应度序列是几乎必然收敛的 ;然后 ,逐渐增加相互作用的强度 ,通过数值... 详细信息

在给出的生态系列演化模型中 ,首先 ,每次只让适应度最小的物种发生变异 ,直到其适应度比原来高为止 ,对此我们给出了每一次变异所需时间的数学期望 ,并证明了最小适应度序列是几乎必然收敛的 ;然后 ,逐渐增加相互作用的强度 ,通过数值模拟表明。

关键词：生物演化模型统计特性数学期望生态系统复杂系统

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miR-483-3p增强胰腺癌对吉西他滨敏感性及分子机制研究

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大连理工大学学报 2025年第1期65卷 30-37页

作者：刘青肖桂山大连理工大学化工学院辽宁大连116024

吉西他滨耐药限制了其对胰腺癌的治疗效果,而传统化学药物无法有效克服吉西他滨对胰腺癌的耐药.研究表明,microRNA(miRNA)的异常表达与肿瘤发生、发展和化疗耐药性有关.对miR-483-3p在胰腺癌吉西他滨耐药中的作用和潜在机制进行了研究.q... 详细信息

吉西他滨耐药限制了其对胰腺癌的治疗效果,而传统化学药物无法有效克服吉西他滨对胰腺癌的耐药.研究表明,microRNA(miRNA)的异常表达与肿瘤发生、发展和化疗耐药性有关.对miR-483-3p在胰腺癌吉西他滨耐药中的作用和潜在机制进行了研究.qPCR结果显示miR-483-3p在胰腺癌耐药细胞中显著下调.miR-483-3p minics瞬时转染显著提高了耐药细胞内miR-483-3p的表达水平,显著减弱了耐药细胞的增殖、迁移、转移和侵袭能力,恢复了吉西他滨的反应性.Western blot结果显示miR-483-3p抑制PI3K和p-AKT蛋白的表达水平,抑制Bcl2蛋白表达,促进Bax、Bad和cleaved-Caspase-3蛋白表达,促进细胞凋亡进程.总之,研究结果表明miR-483-3p通过抑制PI3K/AKT信号通路促进细胞凋亡,增加胰腺癌对吉西他滨的敏感性,miR-483-3p可能是吉西他滨耐药胰腺癌患者的潜在治疗策略.

关键词： miR-483-3p 胰腺癌吉西他滨耐药性 miRNA

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