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Vacuolar processing enzyme positively modulates plant resistance and cell death in response to Phytophthora parasitica infection

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Journal of Integrative Agriculture 2023年第5期22卷 1424-1433页

作者： GAO Xian-xian TANG Ya-ling SHI Qing-yao WEI yu-shu WANG xiao-xue SHAN Wei-xing qiang xiao-yu State Key Laboratory of Crop Stress Biology for Arid Areas/College of Agronomy Northwest A&F UniversityYangling 712100P.R.China

Oomycete, particularly Phytophthora species, causes the most devastating crop diseases, such as potato late blight,and threatens the sustainable crop production worldwide. Our previous studies identified Resistance to Phytophthora parasitica 1(RTP1) as a negative regulator of Arabidopsis resistance to multiple biotrophic pathogens and RTP1 lossof-function plants displayed rapid cell death and reactive oxygen species(ROS) production during early colonization of P. parasitica. In this study, we aim to decipher the mechanism of RTP1-mediated cell death, and identify a member of vaculoar processing enzymes(VPEs), γVPE, playing a role in rtp1-mediated resistance to P. parasitica and cell death occurrence. Our results showed up-regulation of the expression of γVPE as well as increased VPE/caspase 1-like protease activity in P. parasitica-infected rtp1 mutant plants. Besides, we found that the VPE/caspase 1-like protease activity was required for the cell death occurrence in Arabidopsis plants during the infection of P. parasitica as well as rtp1-mediated resistance to P. parasitica. Further pathogenicity assays on either Arabidopsis γvpe mutant plants or leaves of Nicotiana benthamiana with transient overexpression of γVPE demonstrated γVPE could positively affect plant resistance to P. parasitica. Together, our studies suggest that γVPE might function as an important regulator of plant defense and cell death occurrence in response to P. parasitica infection, and VPE/caspase 1-like protease activity is required for rtp1-mediated resistance to P. parasitica.

关键词： cell death caspase 1 plant resistance Phytophthora parasitica RTP1 susceptibility factor vacuolar processingenzyme

Transcriptome-wide analysis of mRNA N^(6)-methyladenosine modification in the embryonic development of Spodoptera frugiperda

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Insect Science 2023年第5期30卷 1229-1244页

作者： Yaqing Chen yushan Lai Runzhou Liu Lin Yao xiao-qiang yu xiaoyun Wang Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina

N^(6)-methyladenosine(m^(6)A)RNA is the most abundant modification of mRNA,and has been demonstrated in regulating various post-transcriptional *** studies have shown that m^(6)A methylation plays key roles in sex determination,neuronal functions,and embryonic development in Drosophila and ***,we analyzed transcriptome-wide profile of m^(6)A modification in the embryonic development of the destructive agricultural pest Spodoptera *** found that the 2 key mRNA m^(6)A methyltransferases SfrMETTL3 and SfrMETTL14 have high homologies with other insects and mammals,suggesting that SfrMETTL3 and SfrMETTL14 may have conserved function among different *** methylated RNA immunoprecipitation sequencing analysis,we obtained 46869 m^(6)A peaks representing 8587 transcripts in the 2-h embryos after oviposition,and 41389 m^(6)A peaks representing 9230 transcripts in the 24-h *** addition,5995 m^(6)A peaks were differentially expressed including 3752 upregulated and 2243 downregulated *** analysis with Gene Ontology and Kyoto Encyclopedia of Genes and Genomes suggested that differentially expressed m^(6)A peak-modified genes were enriched in cell and organ development between the 2-and 24-h *** conjoint analysis of methylated RNA immunoprecipitation-seq and RNA-seq data,we found that RNA m^(6)A methylation may regulate the transcriptional levels of genes related to tissue and organ development from 2-to 24-h *** study reveals the role of RNA m^(6)A epigenetic regulation in the embryonic development of ***,and provides new insights for the embryonic development of insects.

关键词： embryonic cdevelopment N^(6)-methyladenosine RNA methylation Spodopterafrugiperda

Genome-wide identification and comparative analysis of Cry toxin receptor families in 7 insect species with a focus on Spodoptera litura

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Insect Science 2022年第3期29卷 783-800页

作者： Qilin Li Mengge Li Mengyao Zhu Jielai Zhong Liang Wen Jie Zhang Ruonan Zhang qiang Gao xiao-qiang yu yuzhen Lu Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhou510631China College of Biology Hunan UniversityChangsha410082China

Cadherin,aminopeptidase N(APN)and alkaline phosphatase(ALP)have been characterized as Cry *** this study,comparative genomic analysis of the 3 receptor families was performed in 7 *** and APNs are divided into three and eight clades in phylogenetic trees,*** in clade 3 and APNs in clade 1 contain multiple paralogs within each species and most paralogs are located closely in *** melanogaster has expanded APNs in clade 5 and were lowly expressed in *** are divided into 16 clades;they may diverge before holometabolous insect speciation except for BtR and Cad89D-like *** insects from different orders containing BtR orthologs are sensitive to Cryl A or Cry3A,while five species without BtR are insensitive to both *** APNs in clade 1,several ALPs in clade 3,BtR and Cad89D-like genes were highly or moderately expressed in larval midgut of Spodoptera litura and the other six species,and several members in these clades have been identi-•fied as Cry *** of putative *** Cry receptors in the midgut after exposing to Bt toxins were also analyzed.

关键词： alkaline phosphatase aminopeptidase N Bacillus thuringiensis cadherin Cry toxin

Genome-wide identification and characterization of basic helix-loop-helix transcription factors in Spodoptera litura upon pathogen infection

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Insect Science 2022年第4期29卷 977-992页

作者： Jie Zhang Mengyao Zhu Qilin Li Ting Tang Liang Wen Jielai Zhong Ruonan Zhang xiao-qiang yu yuzhen Lu Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina Guangzhou Key Laboratory of Insect Development Regulation and Application Research Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina

Basic helix-loop-helix(bHLH)transcription factors play an important role in a wide range of metabolic and developmental processes in eukaryotes,and bHLH proteins also participate in immune responses,especially in ***,their roles in insects upon entomopathogen infection are *** this study,54 bHLH genes in 41 families were identified in a polyphagous pest,Spodoptera litura,including a new bHLH gene in group B,which is specifically present in Lepidoptera and was thus named *** conserved amino acids in the bHLH domain,structural architecture,and chromosomal distribution of bHLH genes in *** were *** bHLH genes in Plutella xylostella and Apis mellifera were also updated,and genome-wide comparison and phylogenetic analysis of bHLH members in 5 holometabolous insects were *** expression profiles of *** bHLH(SlbHLH)genes in 3 tissues at different developmental stages and their responses to *** nucleopolyhedrovirus(SpltNPV),Nomuraea rileyi(Nr),and Bacillus thuringiensis(Bt)infection were *** SlbHLHs in group B were expressed and differentially expressed during pathogen infections,and SlbHLHs tended to be downregulated in the midgut of *** larvae after *** *** study provides an overview of bHLH family members in *** and their responses to different pathogens used for pest *** findings on bHLH members may contribute to uncovering the mechanism of host-pathogen interaction.

关键词： basic helix-loop-helix entomopathogen infection pest biocontrol Spodoptera liura

BmMD-2A responds to 20-hydroxyecdysone and regulates Bombyx mori silkworm innate immunity in larva-to-pupa metamorphosis

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Insect Science 2023年第2期30卷 411-424页

作者： Ruonan Zhang Xue Chen yuan Wang Xu Bai Qiong Yang Yangjin Zhong xiao-qiang yu Fengliang Jin Wanying Yang Guangdong Laboratory for Lingnan Modern Agriculture Laboratory of Insect Molecular Biology and BiotechnologyGuangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular BreedingCollege of Animal ScienceSouth China Agricultural UniversityGuangzhou510642China Guangdong Laboratory of Bio-Pesticide Creation and Application of Guangdong Province College of Plant ProtectionSouth China Agricultural UniversityGuangzhou510642China The Sericultural and Agri-Food Research Institute of the Guangdong Academy of Agricultural Sciences GuangzhouChina Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina

20E-hydroxyecdysone(20E)plays important roles in larval molting and metamorphosis in insects and is also involved in the insect innate immune *** metamorphosis is a highly successful strategy for environmental adaptation and is the most vulnerable stage during which the insect is susceptible to various pathogens.20E regulates a series of antimicrobial peptides(AMPs)through the immunodeficiency(IMD)pathway activation in Drosophila;nevertheless,whether other immune pathways are involved in 20E-regulated insect immunity is *** previous studies showed that BmMD-2A is a member of the MD-2-related lipid recognition(ML)family of proteins that are involved in the Bombyx mori innate immunity Toll signaling *** this study,we further demonstrate that BmMD-2A is also positively regulated by 20E,and the BmMD-2A neutralization experiment suggested that 20E activates some downstream immune effect factors,the AMP genes against Escherichia coli and Staphylococcus aureus,through the regulation of BmMD-2A in larval metamorphosis,implying that *** may use the Toll-ML signaling pathway to maintain innate immune balance in the larval-pupal metamorphosis stage,which is a different innate immunity pathway regulated by 20E compared to the IMD pathway in Drosophila.

关键词： AMPs BmMD-2A 20E innate immune metamorphosis

Sublethal exposure to spinetoram impacts life history traits and dengue virus replication in Aedes aegypti

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Insect Science 2023年第2期30卷 486-500页

作者： Luoluo Wang Zhichang Pang Qi Chen Zhentao Song Yi Lu Meng Yang Jia Huang xiao-qiang yu xiaoyun Wang Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Instute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina State Key Laboratory of Biocontrol School of Life SciencesSun Yat-sen UniversityGuangzhouChina

Insecticides are anthropogenic environmental stressors and also a common stressor for mosquito ***,the use of insecticides is often guided by short-term efficacy,and the sublethal effect on their target or nontarget species has long been ***,we analyzed how sublethal exposure of the promising vector-control bioinsecticide spinetoram to Aedes aegypti larvae alter adult performance and susceptibility to dengue virus(DENV)*** found that the surviving adult mosquitoes were significantly smaller and exhibited weaker blood-feeding capacity than control females,apart from the extended immature development *** terms of reproductive potential,although the F_(0) generation produced a similar number of eggs and offspring during the first gonotrophic cycle,the survival rates of the F_(1) generations were significantly lower as compared to the control group,suggesting transgenerational sublethal effects on the F_(1) ***,surviving adult females had higher DENV-2 viral loads than the control group after spinetoram sublethal ***,transcriptomic analysis showed that inhibition of oxidative phosphorylation may function in stimulating DENV production in adult *** Aag2 cells,significant accumulation of apoptosis,mitochondrial reactive oxygen species production,and DENV-2 replication by spinetoram exposure consistently support our *** study highlights the threat of sublethal spinetoram exposure on outbreaks of mosquito-borne viruses.

关键词： Aedes aegypti dengue virus spinetoram sublethal exposure susceptibility

Molecular docking-aided AIEgen design: concept, synthesis and applications

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Science China Chemistry 2024年第8期67卷 2614-2628页

作者： Jian-Qing Zhang xiao-yu Xu Fu-Sheng Liu Shu-qiang Cao yu-Xin Gui Yi-Wen Su xiao-yu He Ji-yuan Liang You-Quan Zou Department of Radiation and Medical Oncology Zhongnan Hospital of Wuhan UniversitySchool of Pharmaceutical SciencesWuhan UniversityWuhan 430071China Key Laboratory of Combinatorial Biosynthesis and Drug Discovery Ministry of EducationWuhan UniversityWuhan 430071China Tai Kang Center for Life and Medical Sciences Wuhan UniversityWuhan 430071China Department of Hepatobiliary Pancreatic SurgeryZhongnan Hospital of Wuhan UniversityWuhan 430071China

Aggregate-induced emission luminogens(AIEgens) have been widely used in biological imaging, chemical sensing, and disease treatments. The rational design and construction of AIEgens have received considerable research interests during the last few years. Herein, molecular docking-aided AIEgen design has been reasonably proposed and AIEgen TBQZY with excellent ~1O_(2) generation ability has been synthesized. The newly developed TBQZY could efficiently kill S. epidermidis and methicillinresistant S. epidermidis(MRSE) by tightly binding to bacteria and triggering the accumulation of ~1O_(2) in bacteria. TBQZY specifically regulated the immune system and polarized macrophages from M1 to M2 to accelerate the elimination of biofilm in vivo. In addition, healing acceleration was observed in chronic wounds treated with TBQZY, and side effects were ***, TBQZY had extraordinary potential for combating drug-resistant bacteria in the clinical setting. This research not only provided new concepts for the design of AIEgens, but also shed some lights on the discovery of drugs against drug-resistant bacteria.

关键词： AIEgen molecular docking drug-resistant bacteria biofilm chronic wounds healing

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Constructing oxygen deficiency-rich V_(2)O_(3)@PEDOT cathode for high-performance aqueous zinc-ion batteries

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Rare Metals 2024年第2期43卷 635-646页

作者： Dong-Fei Sun Zi-Juan Wang Tian Tian Xin yu Dan-Dan yu xiao-Zhong Zhou Guo-Fu Ma Zi-qiang Lei Key Laboratory of Eco-functional Polymer Materials of the Ministry of Education Key Laboratory of Eco-environmental Polymer Materials of Gansu ProvinceSchool of College of Chemistry and Chemical EngineeringNorthwest Normal UniversityLanzhou730070China

Aqueous zinc-ion batteries(AZIBs)have attracted widespread attention due to the advantages of high safety and environmental *** V_(2)O_(3) is a promising cathode,the strong electrostatic interaction between Zn^(2+) and V_(2)O_(3) crystal,and the sluggish reaction kinetics still limit their application in ***,the oxygen defects rich V_(2)O_(3) with conducive poly(3,4-ethylenedioxythiophene)(PEDOT)shell(V_(2)O_(3)-Od@PEDOT)was fabricated for AZIBs by combining the sulfur-assisted thermal reduction and in-situ polymerization *** introduced oxygen vacancies of V_(2)O_(3)–Od@PEDOT weaken the electrostatic interaction between Zn^(2+) and the host material,improving the interfacial electron transport,while the PEDOT coating enhances the structural stability and conductivity of V_(2)O_(3),thus accelerating the reaction *** on the advantages,V_(2)O_(3)–Od@PEDOT electrode delivers a reversible capacity of 495 mAh·g^(−1) at 0.1 A·g^(−1),good rate capability(189 mAh·g^(−1)at 8.0 A·g^(−1)),and an impressive cycling stability with 90.1%capacity retention over 1000 cycles at 8.0 A·g^(−1).The strategy may provide a path for exploiting the other materials for high performance AZIBs.

关键词： Oxygen defects V_(2)O_(3) PEDOT Electrochemical self-optimization Aqueous zinc-ion batteries

Micro defects formation and dynamic response analysis of steel plate of quasi-cracking area subjected to explosive load

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Defence Technology（防务技术） 2024年第1期31卷 580-593页

作者： Zheng-qing Zhou Ze-chen Du xiao Wang Hui-ling Jiang qiang Zhou yu-long Zhang yu-zhe Liu Pei-ze Zhang Research Institute of Macro-Safety Science University of Science and TechnologyBeijing100083China School of Civil and Resource Engineering University of Science and Technology BeijingBeijing100083China School of Mechanical Engineering Nantong UniversityNantong226019China China North Industry Advanced Technology Generalization Institute Beijing100089China China Academy of Ordnance Science China

As the protective component,steel plate had attracted extensive attention because of frequently threats of explosive *** this paper,the evolution of microstructure and the mechanism of damage in the quasi-cracking area of steel plate subjected to explosive load were discussed and the relationships between micro defects and dynamic mechanical response were *** the explosion experiment,five observation points were selected equidistant from the quasi-cracking area of the section of the steel plate along the thickness direction,and the characteristics of micro defects at the observation points were analyzed by optical microscope(OM),scanning electron microscope(SEM) and electron backscattered diffraction(EBSD).The observation result shows that many slip bands(SBs) appeared,and the grain orientation changed obviously in the steel plate,the two were the main damage types of micro *** addition,cracks,peeling pits,grooves and other lager micro defects were appeared in the lower area of the *** stress parameters of the observation points were obtained through an effective numerical *** mechanism of damage generation and crack propagation in the quasicracking area were clarified by comparing the specific impulse of each observation point with the corresponding micro *** result shows that the generation and expansion of micro defects are related to the stress area(*** upper compression area,the neutral plane area,and the lower tension area).The micro defects gather and expand at the grain boundary,and will become macroscopic damage under the continuous action of tensile ***,the micro defects at the midpoint of the section of the steel plate in the direction away from the explosion center(*** horizontal direction) were also *** was found that the specific impulse at these positions were much smaller than that in the thickness direction,the micro defects were only SBs and a few micro cracks,and the those decrea

关键词： Explosive load Quasi-cracking area Micro defects Steel plate Dynamic response Numerical simulation