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The plant circadian clock regulates autophagy rhythm through transcription factor LUX ARRHYTHMO

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Journal of Integrative Plant Biology 2022年第11期64卷 2135-2149页

作者： Ming-Kang Yang xiao-jie zhu Chu-Min Chen Xu Guo Shu-Xuan Xu Ya-Rou Xu Shen-Xiu Du Shi xiao Bernd Mueller-Roeber Wei Huang Liang Chen State Key Laboratory for Conservation and Utilization of Subtropical Agro‐Bioresources College of Life SciencesSouth China Agricultural UniversityGuangzhou 510642China Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou 510642China Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms South China Agricultural UniversityGuangzhou 510642China State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources School of Life SciencesSun Yat‐Sen UniversityGuangzhou 510275China Department of Molecular Biology Institute of Biochemistry and BiologyUniversity of Potsdam14476 Potsdam‐GolmGermany Max Planck Institute of Molecular Plant Physiology 14476 Potsdam‐GolmGermany

Autophagy is an evolutionarily conserved degradation pathway in eukaryotes;it plays a critical role in nutritional stress *** circadian clock is an endogenous timekeeping system that generates biological rhythms to adapt to daily changes in the *** evidence indicates that the circadian clock and autophagy are intimately interwoven in ***,the role of the circadian clock in regulating autophagy has been poorly elucidated in ***,we show that autophagy exhibits a robust circadian rhythm in both light/dark cycle(LD)and in constant light(LL)in ***,autophagy rhythm showed a different pattem with a phase-advance shift and a lower amplitude in LL compared to ***,mutation of the transcription factor LUX ARRHYTHMO(LUX)removed autophagy rhythm in LL and led to an enhanced amplitude in *** represses expression of the core autophagy genes ATG2,ATG8 a,and ATG11 by directly binding to their *** analysis revealed that LUX is responsible for improved resistance of plants to carbon starvation,which is dependent on moderate autophagy *** transcriptomic analysis revealed that the autophagy rhythm is ubiquitous in *** together,our findings demonstrate that the LUXmediated circadian clock regulates plant autophagy rhythms.

关键词： autophagy carbon starvation circadian clock LUX rhythm

Cyano migration-mediated radical-polar crossover cyclopropanation

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Science China Chemistry 2025年第1期68卷 241-248页

作者： Ziqiang Wang Yasu Chen jie Li Chen zhu Key Laboratory of Organic Synthesis of Jiangsu Province College of Chemistry Chemical Engineering and Materials ScienceSoochow University Frontiers Science Center for Transformative Molecules Zhangjiang Institute for Advanced Study and Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs Shanghai Jiao Tong University

We report a novel cyano migration-mediated radical-polar crossover process, whereby both the sulfonylcyanation of aliphatic alkenes and the formation of cyclopropanes are concurrently achieved. This transformation yields a diverse array of multisubstituted cyclopropanes under mild photocatalytic conditions. Mechanistic studies elucidate that the reaction proceeds via intramolecular nucleophilic substitution, leading to the formation of the cyclopropane derivatives.

关键词： cyclopropanation cyano migration radical-polar crossover radical reactions

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酸性环境中骨料成分对混凝土桩的影响

中南大学学报（自然科学版） 2016年第10期47卷 3530-3537页

作者：肖杰屈文俊朱鹏同济大学建筑工程系上海200092

为了探寻骨料成分对混凝土抗硫酸腐蚀的影响,进行4种骨料组合(青石+黄砂,青石+大理砂,大理石+黄砂,大理石+大理砂)的混凝土抗硫酸加速腐蚀试验。每天用浓硫酸调节溶液p H保持在0.95左右,试件采用直径×长度为100 mm×200 mm的圆柱体。... 详细信息

为了探寻骨料成分对混凝土抗硫酸腐蚀的影响,进行4种骨料组合(青石+黄砂,青石+大理砂,大理石+黄砂,大理石+大理砂)的混凝土抗硫酸加速腐蚀试验。每天用浓硫酸调节溶液p H保持在0.95左右,试件采用直径×长度为100 mm×200 mm的圆柱体。通过对这4种骨料组合的12个混凝土试件,进行为期194 d的12次跟踪监测,测试计算得到腐蚀深度、长度、质量、吸水率、动弹性模量这5个指标。研究结果表明:粗骨料采用大理石,细骨料采用大理砂,硫酸腐蚀后,混凝土的腐蚀深度小,质量损失小,吸水率小;粗骨料采用青石,细骨料采用大理砂,硫酸腐蚀后,混凝土的动弹性模量降低小;细骨料对腐蚀深度、质量损失、动弹性模量的影响大于粗骨料的影响,粗骨料对于吸水率的影响大于细骨料的影响。

关键词：骨料成分硫酸腐蚀耐久性混凝土

Photoinduced radical sulfur dioxide insertion with asymmetric cyclization of alkenes: accessing β-chiral sulfones bearing S-stereocentric cyclic sulfinamides

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Science China Chemistry 2025年第1期68卷 257-263页

作者： Jiapian Huang Chenxin Wang Xu Wang xiaoyu Zhou Wei xiao jie Wu School of Pharmaceutical and Chemical Engineering & Institute for Advanced Studies Taizhou University State Key Laboratory of Organometallic Chemistry Shanghai Institute of Organic Chemistry Chinese Academy of Sciences School of Chemistry and Chemical Engineering Henan Normal University

The generation of various β-chiral sulfones bearing chiral cyclic sulfinamides through photoredox-catalyzed sulfur dioxide insertion with asymmetric radical cyclization is described. This method features a broad substrate scope and excellent stereospecificity. Computational studies reveal that the dispersion interaction between sulfinyl group and CH2SO2Ph group plays a crucial role in the stereoselectivity control.

关键词： β-chiral sulfones chiral cyclic sulfinamides sulfur dioxide insertion asymmetric radical cyclization stereoselectivity

miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells

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Neural Regeneration Research 2025年第1期20卷 277-290页

作者： Meng Cong Jing-Jing Hu Yan Yu xiao-Li Li xiao-Ting Sun Li-Ting Wang Xia Wu Ling-jie zhu xiao-Jia Yang Qian-Ru He Fei Ding Hai-Yan Shi School of Medicine Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Coinnovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Physiology Jiangsu Health Vocational CollegeNanjingJiangsu ProvinceChina Department of Pathology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

Our previous study found that rat bone marrow–derived neural crest cells(acting as Schwann cell progenitors)have the potential to promote long-distance nerve ***-based therapy can enhance peripheral nerve repair and regeneration through paracrine bioactive factors and intercellular ***,the complex contributions of various types of soluble cytokines and extracellular vesicle cargos to the secretome remain *** investigate the role of the secretome and extracellular vesicles in repairing damaged peripheral nerves,we collected conditioned culture medium from hypoxia-pretreated neural crest cells,and found that it significantly promoted the repair of sensory neurons damaged by oxygen-glucose *** mRNA expression of trophic factors was highly expressed in hypoxia-pretreated neural crest *** performed RNA sequencing and bioinformatics analysis and found that miR-21-5p was enriched in hypoxia-pretreated extracellular vesicles of neural crest ***,to further clarify the role of hypoxia-pretreated neural crest cell extracellular vesicles rich in miR-21-5p in axonal growth and regeneration of sensory neurons,we used a microfluidic axonal dissociation model of sensory neurons in vitro,and found that hypoxia-pretreated neural crest cell extracellular vesicles promoted axonal growth and regeneration of sensory neurons,which was greatly dependent on loaded ***,we constructed a miR-21-5p-loaded neural conduit to repair the sciatic nerve defect in rats and found that the motor and sensory functions of injured rat hind limb,as well as muscle tissue morphology of the hind limbs,were obviously *** findings suggest that hypoxia-pretreated neural crest extracellular vesicles are natural nanoparticles rich in ***-21-5p is one of the main contributors to promoting nerve regeneration by the neural crest cell *** helps to explain the mechanism of action of the secretome and e

关键词： axotomy cell-free therapy conditioned medium extracellular vesicles hypoxic preconditioning microRNA oxygen-glucose deprivation peripheral nerve injury Schwann cell precursors

Effects of the extrusion parameters on microstructure,texture and room temperature mechanical properties of extruded Mg-2.49Nd-1.82Gd-0.2Zn-0.2Zr alloy

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 136-146页

作者： Chenjin Zhang Guangyu Yang Lei xiao Zhiyong Kan Jing Guo Qiang Li Wanqi jie State Key Laboratory of Solidification Processing Northwestern Polytechnical UniversityXi’an 710072China College of Materials Science and Engineering Fuzhou UniversityFuzhou 350108China College of Chemistry and Environmental Science Qujing Normal UniversityQujing 655011China

Microstructure,texture,and mechanical properties of the extruded Mg-2.49Nd-1.82Gd-0.2Zn-0.2Zr alloy were investigated at different extrusion temperatures(260 and 320℃),extrusion ratios(10:1,15:1,and 30:1),and extrusion speeds(3 and 6 mm/s).The experimental results exhibited that the grain sizes after extrusion were much finer than that of the homogenized alloy,and the second phase showed streamline distribution along the extrusion direction(ED).With extrusion temperature increased from 260 to 320℃,the microstructure,texture,and mechanical properties of alloys changed *** dynamic recrystallization(DRX)degree and grain sizes enhanced as the extrusion ratio increased from 10:1 to 30:1,and the strength gradually decreased but elongation(EL)*** the extrusion speed increased from 3 to 6 mm/s,the grain sizes and DRX degree increased significantly,and the samples presented the typical-rare-earth(RE)*** alloy extruded at 260℃ with extrusion ratio of 10:1 and extrusion speed of 3 mm/s showed the tensile yield strength(TYS)of 213 MPa and EL of 30.6%.After quantitatively analyzing the contribution of strengthening mechanisms,it was found that the grain boundary strengthening and dislocation strengthening played major roles among strengthening *** results provide some guidelines for enlarging the industrial application of extruded Mg-RE alloy.

关键词： Mg-rare earth alloys extrusion temperature extrusion ratio extrusion speed strengthening mechanisms

Increased excitatory amino acid transporter 2 levels in basolateral amygdala astrocytes mediate chronic stress–induced anxiety-like behavior

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Neural Regeneration Research 2025年第6期20卷 1721-1734页

作者： Xirong Xu Shoumin Xuan Shuai Chen Dan Liu Qian xiao jie Tu Shenzhen Key Laboratory of Neuroimmunomodulation for Neurological Diseases Shenzhen-Hong Kong Institute of Brain ScienceShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina CAS Key Laboratory of Brain Connectome and Manipulation Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina Guangdong Provincial Key Laboratory of Brain Connectome and Behavior the Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina University of Chinese of Academy of Sciences BeijingChina Faculty of Life and Health Sciences Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina

The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain function and encoding behaviors associated with ***, astrocytes in the basolateral amygdala have been found to play a role in the modulation of anxiety-like behaviors triggered by chronic stress. Nevertheless, the precise molecular mechanisms by which basolateral amygdala astrocytes regulate chronic stress–induced anxiety-like behaviors remain to be fully elucidated. In this study, we found that in a mouse model of anxiety triggered by unpredictable chronic mild stress, the expression of excitatory amino acid transporter 2 was upregulated in the basolateral amygdala. Interestingly, our findings indicate that the targeted knockdown of excitatory amino acid transporter 2 specifically within the basolateral amygdala astrocytes was able to rescue the anxiety-like behavior in mice subjected to stress. Furthermore, we found that the overexpression of excitatory amino acid transporter 2 in the basolateral amygdala, whether achieved through intracranial administration of excitatory amino acid transporter 2agonists or through injection of excitatory amino acid transporter 2-overexpressing viruses with GfaABC1D promoters, evoked anxiety-like behavior in mice. Our single-nucleus RNA sequencing analysis further confirmed that chronic stress induced an upregulation of excitatory amino acid transporter 2 specifically in astrocytes in the basolateral amygdala. Moreover, through in vivo calcium signal recordings, we found that the frequency of calcium activity in the basolateral amygdala of mice subjected to chronic stress was higher compared with normal *** knocking down the expression of excitatory amino acid transporter 2 in the basolateral amygdala, the frequency of calcium activity was not significantly increased, and anxiety-like behavior was ob

关键词： anxiety astrocytes basolateral amygdala behavior dihydrokainic acid excitatory amino acid transporter 2 fiber photometry glutamate LDN-212320 transporter

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泾渭分明的课程壁垒逐渐淡化

全球教育展望 2018年第3期47卷 31-43页

作者：肖驰朱婕胡航舟华东师范大学教育学部华东师范大学课程与教学研究所

在40年的课程改革历程中,课程知识壁垒逐渐消融,学生生活经验越来越多地进入学校课程,课程内容与学生生活联系日益紧密。一方面校本课程、综合实践活动等课程为地方性经验和个人经验等生活知识进入课程体系提供了合法的渠道;另外一方面... 详细信息

在40年的课程改革历程中,课程知识壁垒逐渐消融,学生生活经验越来越多地进入学校课程,课程内容与学生生活联系日益紧密。一方面校本课程、综合实践活动等课程为地方性经验和个人经验等生活知识进入课程体系提供了合法的渠道;另外一方面是传统的学科课程作为学校课程的基础,源源不断吸纳学生个人经验,使得个人知识与学科知识不断融通对话。此外,学校内部的学科互通也越来越频繁,过于强调学科体系的课程结构开始打破,课程的整合程度不断提高。

关键词：课程改革课程整合课程知识生活经验

Trinuclear gold-catalyzed site-selective alkylation of peptides

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Science China Chemistry 2025年第1期68卷 249-256页

作者： Qing-Yun Fang Chengyihan Gu Yinghan Chen Wencheng Yan Yong Liang Weipeng Li Chengjian zhu jie Han Jin Xie State Key Laboratory of Coordination Chemistry Jiangsu Key Laboratory of Advanced Organic Materials Chemistry and Biomedicine Innovation Center (Chem BIC) School of Chemistry and Chemical Engineering Nanjing University Green Catalysis Center College of Chemistry Zhengzhou University State Key Laboratory of Natural Medicines China Pharmaceutical University

Selective modification of peptides has always been a challenging issue. The selective alkylation of peptides can alter the biological activity and physical properties of molecules, and it has gained significant research interest. We herein disclose an intriguing gold-catalyzed alkylation of peptides using bench-stable unactivated alkyl bromides under photoredox catalysis. A wide range of structurally diverse primary, secondary and tertiary alkyl bromides serve as effective coupling partners to precisely connect with the α-C(sp3)–H in glycine residue of peptides. This protocol demonstrates an exceptionally broad scope for alkyl halides and excellent tolerance for a wide range of useful functional groups. A series of relay transformations to construct cyclic peptides, to proceed click reaction and to realize peptide fluorescence labeling further enhance its synthetic practicality. In addition, mechanistic studies and density functional theory(DFT) calculations reveal an inner-sphere and subsequent outersphere single electron transfer(SET) mechanism.

关键词： gold catalysis peptide alkylation C-C coupling radical

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