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Protein and gene expression characteristics of heterogeneous nuclear ribonucleoprotein H1 in esophageal squamous cell carcinoma

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World Journal of Gastroenterology 2016年第32期22卷 7322-7331页

作者： Yu-Lin Sun Fei Liu Fang Liu xiao-hang zhao State Key Laboratory of Molecular Oncology National Cancer Center/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College

AIM To investigate the expression characteristics of heterogeneous nuclear ribonucleoprotein H1(HNRNPH1) m RNA and protein in cell lines and tissues of esophageal squamous cell carcinoma(ESCC). METHODS Western blotting was used to assess the expression of HNRNPH1 protein in seven ESCC cell lines and 30 paired fresh tissue specimens. The subcellular localization of HNRNPH1 was determined by immunofluorescence in ESCC cells. The RNA sequencing data from 87 patients with ESCC were obtained from the cancer genome atlas(TCGA), and the expression and clinical characteristics analysis of different transcript variants of HNRNPH1 were evaluated in this dataset. In addition, immunohistochemistry was carried out to detect the expression of HNRNPH1 protein in 125 *** The expression of HNRNPH1 protein varied across different ESCC cell lines. It was exclusively restricted to the nucleus of the ESCC cells. There are two transcript variants of the HNRNPH1 gene. Variant 1 was constitutively expressed, and its expression did not change during tumorigenesis. In contrast, levels of variant 2 were low in non-tumorous tissues and were dramatically increased in ESCC(P = 0.0026). The high levels of variant 2 were associated with poorer differentiated tumors(P = 0.0287). Furthermore, in paired fresh tissue specimens, HNRNPH1 protein was overexpressed in 73.3%(22/30) of neoplastic tissues. HNRNPH1 was significantly upregulated in ESCC, with strong staining in 43.2%(54/125) of tumor tissues and 22.4%(28/125) of matched non-cancerous tissues(P = 0.0005). Positive HNRNPH1 expression was significantly associated with poor tumor differentiation degree(P = 0.0337).CONCLUSION The different alternative transcript variants of HNRNPH1 exhibited different expression changes during tumorigenesis. Its m RNA and protein were overexpressed in ESCC and associated with poorer differentiation of tumor cells. These findings highlight the potential of HNRNPH1 in the therapy and diagnosis of ESCC.

关键词： Heterogeneous nuclear ribonucleoprotein H1 Esophageal squamous cell carcinoma Alternative transcript variants Biomarker

Novel method for extracting exosomes of hepatocellular carcinoma cells

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World Journal of Gastroenterology 2014年第21期20卷 6651-6657页

作者： Lin Zhu Xiu-Hua Qu Yu-Lin Sun Yang-Ming Qian xiao-hang zhao Third School of Clinical Medicine Southern Medical University Center of Basic Medical Sciences Navy General Hospital of Chinese PLA State Key Laboratory of Molecular Oncology Cancer Institute and HospitalChinese Academy of Medical Sciences and Peking Union Medical College

AIM: To develop a novel method for the rapid and efficient extraction of exosomes secreted by tumor cells.

关键词： Exosome Membrane vesicles Tissue transglutaminase 2 Annexin A2 Nanomaterials

Aberrant expression of peroxiredoxin 1 and its clinical implications in liver cancer

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World Journal of Gastroenterology 2015年第38期21卷 10840-10852页

作者： Yu-Lin Sun Jian-Qiang Cai Fang Liu Xin-Yu Bi Lan-Ping Zhou xiao-hang zhao State Key Laboratory of Molecular Oncology Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical College Department of Abdominal Surgery Cancer HospitalChinese Academy of Medical Science and Peking Union Medical College

AIM: To investigate the expression characteristics of peroxiredoxin 1(PRDX1) m RNA and protein in liver cancer cell lines and ***: The RNA sequencing data from 374 patients with liver cancer were obtained from The Cancer Genome Atlas. The expression and clinical characteristics of PRDX1 m RNA were analyzed in this dataset. The Kaplan-Meier and Cox regression survival analysis was performed to determine the relationship between PRDX1 levels and patient survival. Subcellular fractionation and Western blotting were used to demonstrate the expression of PRDX1 protein in six liver cancer cell lines and 29 paired fresh tissue specimens. After bioinformatics prediction,a putative posttranslational modification form of PRDX1 was observed using immunofluorescence under confocal microscopy and immunoprecipitation analysis in liver cancer ***: The m RNA of PRDX1 gene was upregulated about 1.3-fold in tumor tissue compared with the adjacent non-tumor control(P = 0.005). Its abundance was significantly higher in men than women(P < 0.001). High levels of PRDX1 m RNA were associated with a shorter overall survival time(P =0.04) but not with recurrence-free survival. The Cox regression analysis demonstrated that patients with high PRDX1 m RNA showed about 1.9-fold increase of risk for death(P = 0.03). In liver cancer cells,PRDX1 protein was strongly expressed with multiple different bands. PRDX1 in the cytosol fraction existed near the theoretical molecular weight,whereas two higher molecular weight bands were present in the membrane/organelle and nuclear fractions. Importantly,the theoretical PRDX1 band was increased,whereas the high molecular weight form was decreased in tumor tissues. Subsequent experiments revealed that the high molecular weight bands of PRDX1 might result from the post-translational modification by small ubiquitin-like modifier-1(SUMO1).CONCLUSION: PRDX1 was overexpressed in the tumor tissues of liver cancer and served as an independent p

关键词： Peroxiredoxin 1 Liver cancer Prognostic factor Pos

Research on Preparation of the Composite Materials LiFePO4-Li3V2（PO4）3

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International Journal of Technology Management 2014年第4期 98-100页

作者： Haoyu JIANG Jianrong xiao hang zhao College of Science Guilin University of Technology Guilin Guangxi 541006 China

The article developed a lithium iron phosphate - composite cathode material of lithium vanadium phosphate. Using X-ray diffraction （XRD）, electronic scanning electron microscopy surface （SEM）, laser particle size analyzer, carbon and sulfur analyzer, and X-ray photoelectron spectroscopy, etc. for the prepared composites were characterized and found the material is mainly crystalline structure of lithium iron phosphate, and lithium vanadium, wherein a small amount of impurities; finer particle size of the material, the particle size distribution is narrow and uniform, smooth particle surface, wrapping in good carbon composite with other materials prepared in comparison the case has a carbon content of about optimum conductivity. To assemble the material into a cell after the 0.1C, IC, 2C when and 5C, the first discharge capacity were 160,145,127 and 109 mA·h·g^-1, after 50 cycles, the discharge capacity of 162, respectively, 144,126 and 106 mA·h·g^-1, which showed good rate characteristics and cycle characteristics.

关键词： Lithium iron phosphate lithium vanadium phosphate graphene composite materials

Identification of biomarkers for hepatocellular carcinoma by semiquantitative immunocytochemistry

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World Journal of Gastroenterology 2014年第19期20卷 5826-5838页

作者： Hong Mu Kai-Xuan Lin Hong zhao Shu Xing Cong Li Fang Liu Hai-Zhen Lu Ze Zhang Yu-Lin Sun Xi-Yun Yan Jian-Qiang Cai xiao-hang zhao State Key Laboratory of Molecular Oncology Cancer Institute HospitalChinese Academy of Medical Sciences and Peking Union Medical College Center of Basic Medical Sciences Navy General Hospital of Chinese PLA Department of Abdominal Surgery Cancer Institute HospitalChinese Academy of Medical Science and Peking Union Medical College Key Laboratory of Protein and Peptide Pharmaceutical National Laboratory of BiomacromoleculesInstitute of BiophysicsChinese Academy of Sciences Department of Pathology Cancer Institute HospitalChinese Academy of Medical Sciences and Peking Union Medical College

AIM: To investigate the expression of key biomarkers in hepatoma cell lines, tumor cells from patients’ blood samples, and tumor tissues.

关键词： Hepatocellular carcinoma Biomarker Immunocytochemistry Semiquantitative analysis Three-dimensional reconstruction

Clinical significance of serum expression of GROβ in esophageal squamous cell carcinoma

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World Journal of Gastroenterology 2011年第21期17卷 2658-2662页

作者： Qiao-Mei Dong Jin-Qiang Zhang Qian Li Jacqueline C Bracher Denver T Hendricks xiao-hang zhao State Key Laboratory of Molecular Oncology Cancer Institute and Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100021 China Center of Basic Medical Sciences Navy General Hospital Beijing 100048 China Division of Medical Biochemistry Faculty of Health Sciences University of Cape Town Cape Town 7925 South Africa

AIM:To determine the association between serum levels of growth-related gene product β(GROβ) and clinical parameters in esophageal squamous cell carcinoma(ESCC).METHODS:Using enzyme-linked immunosorbent assay,serum GROβ levels were measured in ESCC patients(n = 72) and healthy volunteers(n = 83).The association between serum levels of GROβ and clinical parameters of ESCC was analyzed ***:The serum GROβ levels were much higher in ESCC patients than in healthy controls(median:645 ng/L vs 269 ng/L,P < 0.05).Serum GROβ levels were correlated positively with tumor size,lymph node metastasis,and tumor-node-metastasis(TNM) staging,but not with gender or the histological grade of tumors in ESCC *** sensitivity and specificity of the assay for serum GROβ were 73.61% and 56.63%,***:GROβ may function as an oncogene product and contribute to tumorigenesis and metastasis of ESCC.

关键词： GROβ Esophageal squamous cell carcinoma Metastasis Cytokine Tumor markers

细胞重要分子表型与肿瘤个体化治疗的研究进展

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世界华人消化杂志 2008年第16期16卷 1781-1787页

作者：何平赵晓航中国医学科学院北京协和医学院肿瘤医院肿瘤研究所分子肿瘤学国家重点实验室北京市100021

恶性肿瘤严重危害着人类的健康.尽管肿瘤的病因学研究取得了一定进展,肿瘤的临床处置依然面临挑战,包括手术切除、化学药物治疗和放射治疗在内的标准治疗模式不能对所有患者都奏效.如何选择合适的治疗方案和准确评估患者的预后,临床医... 详细信息

恶性肿瘤严重危害着人类的健康.尽管肿瘤的病因学研究取得了一定进展,肿瘤的临床处置依然面临挑战,包括手术切除、化学药物治疗和放射治疗在内的标准治疗模式不能对所有患者都奏效.如何选择合适的治疗方案和准确评估患者的预后,临床医生经常感到困惑.针对信号通路重要蛋白的分子靶向治疗的结果显示,不同肿瘤患者具有独特的分子表型特征并决定了肿瘤治疗的疗效.有效治疗肿瘤需要量体裁衣式地设计出个体化的治疗方案.通过"组"学研究发现了许多肿瘤细胞特异的分子表型,而针对这些表型的个体化治疗将是未来肿瘤治疗的方向.

关键词：分子表型个体化治疗蛋白质组学肿瘤标志信号通路

Constructing Donor–Acceptor‑Linked COFs Electrolytes to Regulate Electron Density and Accelerate the Li^(+)Migration in Quasi‑Solid‑State Battery

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Nano-Micro Letters 2025年第1期17卷 456-471页

作者： Genfu zhao hang Ma Conghui Zhang Yongxin Yang Shuyuan Yu Haiye Zhu Yongjiang Sun Hong Guo School of Materials and Energy International Joint Research Center for Advanced Energy Materials of Yunnan ProvinceYunnan UniversityKunming 650091People’s Republic of China Yunnan Yuntianhua Co. LtdR&D CenterKunming 650228People’s Republic of China Southwest United Graduate School Kunming 650091People’s Republic of China

Regulation the electronic density of solid-state electrolyte by donor–acceptor(D–A)system can achieve highly-selective Li^(+)transportation and conduction in solid-state Li metal *** study reports a high-performance solid-state electrolyte thorough D–A-linked covalent organic frameworks(COFs)based on intramolecular charge transfer *** other reported COFbased solid-state electrolyte,the developed concept with D–A-linked COFs not only achieves electronic modulation to promote highly-selective Li^(+)migration and inhibit Li dendrite,but also offers a crucial opportunity to understand the role of electronic density in solid-state Li metal *** introduced strong electronegativity F-based ligand in COF electrolyte results in highlyselective Li^(+)(transference number 0.83),high ionic conductivity(6.7×10^(-4)S cm^(−1)),excellent cyclic ability(1000 h)in Li metal symmetric cell and high-capacity retention in Li/LiFePO_(4)cell(90.8%for 300 cycles at 5C)than substituted C-and N-based *** is ascribed to outstanding D–A interaction between donor porphyrin and acceptor F atoms,which effectively expedites electron transferring from porphyrin to F-based ligand and enhances Li^(+)***,we anticipate that this work creates insight into the strategy for accelerating Li^(+)conduction in high-performance solid-state Li metal batteries through D–A system.

关键词： Electronic modulation engineering Donor-acceptor-linked covalent organic frameworks Quasi-solid-state Li metal battery

Monitoring disease progression and treatment efficacy with circulating tumor cells in esophageal squamous cell carcinoma: A case report

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World Journal of Gastroenterology 2015年第25期21卷 7921-7928页

作者： Yuan-Yuan Qiao Kai-Xuan Lin Ze Zhang Da-Jin Zhang Cheng-He Shi Ming Xiong Xiu-Hua Qu xiao-hang zhao Center for Basic Medical Sciences Navy General Hospital of Chinese PLA Graduate School of Southern Medical University State Key Laboratory of Molecular Oncology Cancer Institute and Hospital Chinese Academy of Medical Sciences and Peking Union Medical College

This study investigated whether changes in circulating tumor cell(CTC) numbers reflect tumor progression and treatment efficacy in esophageal squamous cell carcinoma(ESCC). A 47-year-old male patient with ESCC is presented in this case study. The patient was evaluated for a series of serum tumor markers and subjected to radiological examinations before and after surgery and during follow-up over the course of five years. In addition, the CTCs in 7.5 m L of peripheral blood were enriched by magneticactivated cell sorting negative selection and identified by immunofluorescence staining. Serum tumor markers remained within normal ranges and were discordant with imaging scans during the follow-up. Initially, one CTC was detected in the peripheral blood sample, and 14 were observed seven days after the operation. After 12 wk, subcutaneous metastases and bone metastases occurred, and the number of CTCs increased to 84. After 48 wk, lung metastases were noted, and the CTC level was 21. At 104 wk, the number of CTCs was 14, and disease recurrence was detected by positron emission tomography-computed tomography. The CTC counts were in accord with the imaging studies at several time points. The additional information provided by CTC enumeration could thus facilitate monitoring of disease status and treatment efficacy and provide support for treatment decisions.

关键词： Individualized treatment Adjuvant therapy Esophageal squamous cell carcinoma Circulating tumorcells