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Growth Inhibition Effect of Parthenolide on Human Hepatocellular Carcinomacell Line BEL-7402

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Chemical Research in Chinese Universities 2006年第2期22卷 173-176页

作者： SONG xiao-kai ZHANG Wen College of Biology and Chemical Engineering Tianjin Institute of Technology Tianjin 300191 P. R. China

To study the effects of parthenolide on human hepatocellular carcinoma cell line BEL-7402 and explore its possible mechanism, human hepatocellular carcinoma cell line BEL-7402 was cultured in vitro and treated with parthenolide of different concentrations. Cells without addition of parthenolide were used as control. The growth of inhibition of cells induced by various concentrations was analyzed by using the MTT assay. The morphologic changes of apoptosis was observed under an inversion microscope by Giemsa staining. The expression levels of PCNA albumen were measured by means of immunohistochemical methods. Parthenolide can inhibit the proliferation of BEL-7402 cells in vitro in adose-dependent manner. Apoptosis with nuclear chromatin condensation, fragmentation and cell shrinkage were found by means of inversion microscopy. Formation of apoptotic bodies was observed by Giemsa staining. The immunohistochemical results show that the expression of PCNA has been decreased. Parthenolide can inhibit the tumor growing of BEL-7402 cells, and induce the apoptosis of cells. The mechanisms of those functions may be via inhibiting the expression of PCNA.

关键词： Parthenolide Human hepatocellular carcinomacell line PCNA

Cloning and Characterization of a Novel Purple Acid Phosphatase Gene （MtPAP1） from Medicago truncatula Barrel Medic

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Journal of Integrative Plant Biology 2006年第2期48卷 204-211页

作者： kai xiao Maria Harrison Zeng-Yu Wang College of Agronomy Agricultural University of Hebei Baoding 071001 China Boyce Thompson Institute for Plant Research Cornell University Ithaca NY 14853-1801 USA Forage Improvement Division The Samuel Roberts Noble Foundation Ardmore OK 73401 USA

A novel purple acid phosphatase gene （MtPAP1） was Isolated from the model legume Medicago truncatula Barrel Medic. The cDNA was 1 698 bp In length with an open reading frame （ORF） of 1 398 bp capable of encoding an N-terminal signal peptlde of 23 amino acids. The transcripts of MtPAP1 were mainly detected In leaves under high-phosphate conditions, whereas under low-phosphate conditions the transcript level was reduced In leaves and Increased In roots, with the strongest hybridization signal detected In roots. A chimeric gene construct fusing MtPAP1 and GFPwas made In which the fusion was driven by the CaMV35S promoter. Transgenlc Arabidopsis plants carrying the chimeric gene constructs showed that the fusion protein was mainly located at the apoplast based on confocal mlcroecoplc analysis, showing that MtPAP1 could be secreted to the outside of the cell directed by the signal peptlde at the N-terminal. The coding region of MtPAP1 without signal peptlde was Inserted Into the prokaryotlc expression vector pET-30a （＋） and overexpressed In Escherichla coil BL21 （DE3）. The acid phosphatase （APase） proteins extracted from bacterial culture were found largely based on sodium dodecyl sulfate-polyecrylamlde gel electrophoresls. An enzyme activity assay demonstrated that the APase activity In the transformed bacteria was 3.16-fold higher than that of control. The results Imply that MtPAP1 functions to Improve phosphorus acquisition In plants under conditions of phosphorus （P） stress.

关键词： gene cloning gene expression MtPAP1.

Full-domain collaborative deployment method of multiple interference sources and evaluation of its deployment effect

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Defence Technology（防务技术） 2024年第2期32卷 572-595页

作者： Yue Wang Fuping Sun Xian Wang Jinming Hao kai xiao School of Geospatial Information The PLA Information Engineering UniversityZhengzhou 450001HenanChina

This paper realizes the full-domain collaborative deployment of multiple interference sources of the global satellite navigation system(GNSS)and evaluates the deployment effect to enhance the ability to disturb the attacker and the capability to defend the GNSS during navigation *** evaluation indicators for the jamming effect of GNSS suppressive and deceptive jamming sources are first created,their evaluation models are built,and their detection procedures are sorted out,as the basis for determining the deployment *** principles for collaboratively deploying multi-jamming sources are developed to obtain the deployment structures(including the required number,structures in demand,and corresponding positions)of three single interference sources required by collaboratively ***,simulation and hardware-in-loop testing results are presented to determine a rational configuration of the collaborative deployment of multi-jamming sources in the set situation and further realize the full-domain deployment of an interference network from ground,air to *** evaluation indices for the deployment effect are finally developed to evaluate the deployment effect of the proposed configuration and further verify its reliability and rationality.

关键词： Jamming effect Multiple interference sources Collaborative deployment Effect evaluation Defense capability

Molecular Characterization and Expression Analysis of TaZFP15, a C_2H_2-Type Zinc Finger Transcription Factor Gene in Wheat (Triticum aestivum L.)

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Journal of Integrative Agriculture 2012年第1期11卷 31-42页

作者： SUN Zhao-hua DING Chang-huan LI xiao-juan xiao kai College of Life Science Agricultural University ofHebei Baoding 071001 P.R.China College of Agronomy Agrieultural University of Hebei Baoding 071001 P.R. China

Based on sequencing of part clones in a root subtractive cDNA library, an expressed sequence tag （EST） sharing high similarity to a rice C2H2 zinc finger transcription factor （ZFP15） was obtained in wheat. Through bioinformatics approach, the wheat C2H2-type ZFP gene referred to TaZFP15 has been identified and characterized. As a full-length cDNA of 670 bp, TaZFP15 has an open reading frame of 408 bp and encodes a 135-aa polypeptide. TaZFP15 contains two C2H2 zinc finger domains and each one has a conserved motif QALGGH. The typical L-box, generally identified in the C2H2 type transcription factors, has also been found in TaZFP15. Phylogenetic analysis suggested that TaZFP15 shares high similarities with rice ZFP15 （GenBank accession no. AY286473）, maize ZFP （GenBank accession no. NM_001159094） and a subset of other zinc-finger transcription factor genes in plant species. The expression of TaZFP15 was up-regulated by starved-Pi stress, showing a pattern to be gradually elevated along with the progression of the Pi-stress in a 23-h treatment regime. Similarly, the transcripts of TaZFP15 in roots were also induced by nitrogen deficiency, and abiotic stresses of drought and salinity. No responses of TaZFP15 were detected in roots to nutrition deficiencies of P, Zn, and Ca, and the external treatment of abscisic acid （ABA）. TaZFP15 could be specifically amplified in genome A, B, and D, and without variability in the sequences, suggesting that TaZFP15 has multi-copies in the homologous hexaploid species. Transgenic analysis in tobacco revealed that up-regulation of TaZFP15 could significantly improve plant dry mass accumulation via increasing the plant phosphorus acquisition capacity under Pi-deficiency condition. The results suggested that TaZFP15 is involved in mediation of signal transductions of diverse external stresses.

关键词： Triticum aestivum L. zinc-finger transcription factor gene nutrition deficiency abiotic stress expression pattern

Distribution Characteristics of Minerals and Elements in Chromite Ore Processing Residue

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Transactions of Tianjin University 2012年第1期18卷 52-56页

作者： LI Guocheng xiao kai School of Civil Engineering and Mechanics Huazhong University of Science and TechnologyWuhan 430074China

In this paper, environmental scanning electron microscopy （ESEM） is applied to characterizing the mineral and element distribution of chromite ore processing residue （COPR）. The test results show that Cr-bearing brownmillerite occurs in the rim of COPR particle, while hydroandradite with Cr （Ⅵ） in its structure presents inside the COPR particle. Periclase and calcite occur in the interstitial area. Element analyses show that Ca, Fe and Al are distributed throughout the COPR particle, and Mg exists mostly in the interstitial area or on the particle surface. A lower content of Cr is evenly distributed in the COPR particle, while slightly higher concentration of Cr occurs inside the particle. It is suggested that it will take a relatively longer time for Cr to migrate out of COPR, especially fbr hexavalent chromium, so the leaching time and the particle size may be two important factors to affect the release of Cr （Ⅵ）.

关键词： chromium chromite ore processing residue scanning electron microscopy mineralogy element distribution

Synthesis, Structure and Electrochemistry of Macrocyclic 1,1'-Bis(1,3-phenyleneoxyacetylpyrazoyl)ferrocene

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Chinese Journal of Structural Chemistry 2007年第5期26卷 505-510页

作者： ZHU Mei-Jun SONG xiao-kai SUN Jing YAN Chao-Guo Department of Civil Engineering Jianghai Polytechnic College Yangzhou 225101 China College of Chemistry ＆ ChemiealEngineering Yangzhou University Yangzhou 225002 China

1,1＇-Diacetoacetylferrocene 1 reacted with phenylene-1,3-dioxyactyl hydrazine 2 in absolute ethanol to give the macrocyclic ferrocenyl dipyrazole compound in moderate yield. Determined by X-ray structure analysis, it crystallizes in monoclinic system, space group P21/c with a = 13.7509（4）, b = 8.1277（2）, c = 21.7472（6） A, β = 103.1030（10）°, V = 2367.25（11） A^3, Z = 4, Dc = 1.505 g/cm^3, R = 0.0353 and wR = 0.0811. The electrochemical studies reveal that redox of Fe^＋/Fe in ferrocene is a reversible one-electron process.

关键词： ferrocene acetylacetone pyrazole cyclic voltammetry

Growth traits and nitrogen assimilation-associated physiological parameters of wheat(Triticum aestivum L.) under low and high N conditions

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Journal of Integrative Agriculture 2015年第7期14卷 1295-1308页

作者： ZHANG Fei-fei GAO Si ZHAO Yuan-yuan ZHAO xiao-lei LIU xiao-man xiao kai College of Agronomy Agricultural University of Hebei

In this study, 14 wheat cultivars with contrasting yield and N use efficiency （NUE） were used to investigate the agronomic and NUE-related traits, and the N assimilation-associated enzyme activities under low and high N conditions. Under deficient-N, the cultivars with high N uptake efficiency （UpE） and high N utilization efficiency （UtE） exhibited higher plant biomass, yields, and N contents than those with medium and low NUEs. The high UpE cultivars accumulated more N than other NUE type cultivars. Under sufficient-N, the tested cultivars showed similar patterns in biomass, yield, and N content to those under deficient-N, but the varietal variations in above traits were smaller. In addition, the high UpE cultivars displayed much more of root biomass and larger of root length, surface area, and volume than other NUE type cultivars, indicating that the root morphological traits under N deprivation are closely associated with the plant biomass through its improvement of the N acquisition. The high UtE cultivars showed higher activities of nitrate reductase （NR）, nitrite reductase （NIR）, and gluta- mine synthetase （GS） at stages of seediling, heading and filling than other NUE type cultivars under both low and high N conditions. Moreover, the high UpE and UtE cultivars also displayed higher photosynthetic rate under deficient-N than the medium and low NUE cultivars. Together, our results indicated that the tested wheat cultivars possess dramatically genetic variations in biomass, yield, and NUE. The root morphological traits and the N assimilation enzymatic acitivities play critical roles in regulating N accumulation and internal N translocation under the N-starvation stress, respectively. They can be used as morphological and biochemical references for evaluation of UpE and UtE in wheat.

关键词： wheat (Triticum aestivum L.) low and high N biomass yield nitrogen use efficiency root morphological traits,N assimilzation-associated enzyme photosynthetic rate

Impact of transcranial electrical stimulation on serum neurotrophic factors and language function in patients with speech disorders

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World Journal of Clinical Cases 2024年第10期12卷 1742-1749页

作者： Li Sun kai xiao xiao-Yan Shen Shu Wang Department of Rehabilitation Medicine General Hospital of the Yangtze River ShippingWuhan 430010Hubei ProvinceChina

BACKGROUND Speech disorders have a substantial impact on communication abilities and quality of *** treatments such as speech and psychological therapies frequently demonstrate limited effectiveness and patient *** electrical stimulation(TES)has emerged as a promising non-invasive treatment to improve neurological ***,its effectiveness in enhancing language functions and serum neurofactor levels in individuals with speech disorders requires further *** To investigate the impact of TES in conjunction with standard therapies on serum neurotrophic factor levels and language function in patients with speech *** In a controlled study spanning from March 2019 to November 2021,81 patients with speech disorders were divided into a control group(n=40)receiving standard speech stimulation and psychological intervention,and an observation group(n=41)receiving additional *** study assessed serum levels of ciliary neurotrophic factor(CNTF),glial cell-derived neurotrophic factor(GDNF),brainderived neurotrophic factor(BDNF),and nerve growth factor(NGF),as well as evaluations of motor function,language function,and development quotient *** After 3 wk of intervention,the observation group exhibited significantly higher serum levels of CNTF,GDNF,BDNF,and NGF compared to the control ***,improvements were noted in motor function,cognitive function,language skills,physical abilities,and overall development quotient *** is worth mentioning that the observation group also displayed superior perfor CONCLUSION This retrospective study concluded that TES combined with traditional speech and psychotherapy can effectively increase the levels of neurokines in the blood and enhance language function in patients with speech *** results provide a promising avenue for integrating TES into standard treatment methods for speech disorders.

关键词： Transcranial electrical stimulation Serum neurofactor levels Developmental level Language features

Expression pattern and function analyses of the MADS thranscription factor genes in wheat(Triticum aestivum L.) under phosphorusstarvation condition

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Journal of Integrative Agriculture 2016年第8期15卷 1703-1715页

作者： SHI Shu-ya ZHANG Fei-fei GAO Si xiao kai Key Laboratory of Crop Growth Regulation of Hebei Province/College of Agronomy Agricultural University of Hebei

supported by the National Natural Science Foundation of China （31201674 and 31371618）;the National Transgenic Major Program, China （2011ZX08008）

关键词： wheat(Triticum aestivum L) MADS type transcription factor Pi deprivation expression gene function