检索结果-南通市图书馆

在线全文

学校读者我要写书评

暂无评论

贵州小型猪腭裂动物实验模型的建立

遵义医学院学报 2007年第1期30卷 23-24页

作者：佘小明田锟钱宁吴曙光赵菊花贵州省贵阳市口腔医院口腔颌面外科贵阳中医学院动物研究所贵州贵阳550002

目的利用贵州小型猪建立人工不完全性腭裂动物实验模型。方法在贵州小型猪的的硬腭上人工制造0.5cm×2.5 cm的骨缺损,术后观察硬组织和软组织的愈合情况。结果观察9mo:刀口愈合良好,裂隙保持完好,有呛食、腭裂音等先天性腭裂症状。结论... 详细信息

目的利用贵州小型猪建立人工不完全性腭裂动物实验模型。方法在贵州小型猪的的硬腭上人工制造0.5cm×2.5 cm的骨缺损,术后观察硬组织和软组织的愈合情况。结果观察9mo:刀口愈合良好,裂隙保持完好,有呛食、腭裂音等先天性腭裂症状。结论利用小型猪建立的腭裂动物模型可靠。

关键词：腭裂贵州小型猪动物模型

SURF1基因604G→C杂合性错义突变所致Leigh综合征患儿的临床与分子遗传学研究

在线全文

学校读者我要写书评

暂无评论

中华神经科杂志 2005年第9期38卷 560-564页

作者：孙芳杨艳玲戚豫王朝霞袁云宋金青钱宁姜玉武肖江喜王霄英王丽秦炯吴希如北京大学第一医院儿科 100034 北京大学第一医院中心实验室 100034 北京大学第一医院神经内科 100034 北京大学第一医院医学影像科 100034

目的研究因常染色体SURF1基因突变所致Leigh综合征患儿及其家系的临床与分子遗传学特点。方法收集39例Leigh综合征患儿及其家属的外周血白细胞脱氧核糖核酸(DNA),运用聚合酶链反应(PCR)扩增SURF1基因的全部外显子序列,进行正反向序列测... 详细信息

目的研究因常染色体SURF1基因突变所致Leigh综合征患儿及其家系的临床与分子遗传学特点。方法收集39例Leigh综合征患儿及其家属的外周血白细胞脱氧核糖核酸(DNA),运用聚合酶链反应(PCR)扩增SURF1基因的全部外显子序列,进行正反向序列测定检测突变,采用限制性片段长度多态性(RFLP)分析验证测序结果,并与100名健康成人对照。结果39例Leigh综合征患儿中有5例(12.8%)SURF1基因外显子7存在604G→C杂合性错义突变。5例患儿(男3例,女2例)均因智力或运动障碍于出生后8个月至9.8年来院就诊。其中3例患儿的父母接受了SURF1基因突变分析,发现双亲中一方SURF1基因存在604G→C杂合性错义突变,而另一方及健康对照的相关外显子序列未发现异常。结论我们首次报道了5例SURF1基因604G→C杂合性错义突变导致Leigh综合征的患儿及其家系,频度高达12.8%,提示该突变可能是中国人的热点突变。我们的研究将有助于今后Leigh综合征患者的诊断和遗传咨询。

关键词： Leigh病蛋白质类突变,误义电子传递复合物Ⅳ Leigh综合征错义突变 SURF 杂合性 1基因遗传学研究

鹰嘴豆芽素A对Hela细胞增殖、迁移及Bcl-2、Bax基因表达的影响

在线全文

学校读者我要写书评

暂无评论

海南医学院学报 2019年第11期25卷 805-809页

作者：赵凤婷王朋博王冰张宁薛钊孙志彬原文政姬国杰丰慧根胡焕焕新乡医学院三全学院生命科学技术学院河南新乡453000 新乡医学院生命科学技术学院河南新乡453000 新乡医学院三全学院生物与医学实验教学中心河南新乡453000

目的:探讨鹰嘴豆芽素A对宫颈癌细胞增殖及迁移能力的调控作用及其可能的分子机制。方法:体外培养Hela细胞,以不同质量浓度的鹰嘴豆芽素A处理宫颈癌Hela细胞,CCK-8法检测鹰嘴豆芽素A对宫颈癌细胞的增殖影响;划痕实验检测对细胞迁移的影响... 详细信息

目的:探讨鹰嘴豆芽素A对宫颈癌细胞增殖及迁移能力的调控作用及其可能的分子机制。方法:体外培养Hela细胞,以不同质量浓度的鹰嘴豆芽素A处理宫颈癌Hela细胞,CCK-8法检测鹰嘴豆芽素A对宫颈癌细胞的增殖影响;划痕实验检测对细胞迁移的影响;RT-PCR法检测鹰嘴豆芽素A对Bcl-2/Bax mRNA水平表达的变化。结果:鹰嘴豆芽素A能够抑制Hela细胞的增殖和迁移,呈现剂量和时间依赖性(P<0.05);质量浓度为10、20、40、80、160μmol/L鹰嘴豆芽素A处理Hela细胞24h、48h后,抑制Hela细胞增殖和迁移速率明显上升,并且高于对照组(P<0.05);促凋亡蛋白Bax表达水平升高,抗凋亡蛋白Bcl-2表达水平降低,呈剂量依赖性。结论:鹰嘴豆芽素A能够抑制宫颈癌Hela细胞的增殖和迁移,其作用机制可能与下调Bcl-2,上调Bax有关。

关键词：鹰嘴豆芽素A 宫颈癌 Bcl-2 Bax

Rare variants play critical role in maize ear genetic architecture revealed by Random-Open-parents association mapping population

在线全文

学校读者我要写书评

暂无评论

Rare variants play critical role in maize ear genetic archit...

第六届全国动植物数量遗传学学术研讨会

作者： Yingjie xiao Hao Tong xiaohong Yang Shizhong Xu Qingchun Pan Feng Qiao Mohammad Sharif Raihan Haijun Liu Xuehai Zhang ning Yang Xiaqing Wang Min Deng Minliang Jin Lijun zhao Xin Luo Yang Zhou Xiang Li Jie Liu Wei Zhan Nannan Liu Hong Wang Genshen Chen Ye Cai Gen Xu Weidong Wang Debo Zheng Jianbing Yan National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University National Maize Improvement Center of China Beijing Key Laboratory of Crop Genetic ImprovementChina Agricultural University Department of Botany and Plant Sciences University of California

The improvement of grain yield is an essential long-term goal for maize breeders to meet continual and increasing food demands *** dissected the genetic architecture of maize ear traits with a set of 10 independent RIL(recombination inbred lines)populations(maize Random-Open-parents Association Mapping population,ROAM).We found that dozens of minor to moderate effect loci influence ear traits,

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

金家油田微生物稠油降黏剂的研制与评价

科学技术与工程 2021年第28期21卷 12088-12093页

作者：毛振强文小林韩春梅于红军黄文欢赵淑霞齐义彬韩佳均中国石化胜利油田分公司纯梁采油厂博兴256504 中国石油化工股份有限公司石油勘探开发研究院北京102206 中国石化胜利油田分公司石油开发中心东营257091

针对金家油田通38块稠油多轮次热采后经济效益变差的现状,开展了采用稠油冷采开发方式的技术探索。稠油冷采技术的核心是研发一种适合目标油藏稠油的低成本高效降黏剂。从油田采出液中筛选到一株能够高产生物表面活性剂菌株Q18,以Q18的... 详细信息

针对金家油田通38块稠油多轮次热采后经济效益变差的现状,开展了采用稠油冷采开发方式的技术探索。稠油冷采技术的核心是研发一种适合目标油藏稠油的低成本高效降黏剂。从油田采出液中筛选到一株能够高产生物表面活性剂菌株Q18,以Q18的发酵代谢产物为基础构建了一种稠油降黏剂RF180。RF180既具有良好的温度和矿化度耐受性,也有较宽的酸碱耐受性,在20~180℃﹑0~120000 mg/L矿化度和pH 5~11的范围内,具有良好的表面及降黏活性,能够适应金家油田的油藏条件。同时,进一步研究发现浓度大于0.3%的RF180对通38稠油就具有良好的乳化与静置降黏效果,乳化与静置降黏率分别达到99.93%和96.64%。物理模拟驱油实验证明,RF180能够大幅提高通38稠油的驱油效率,在不同注入方式条件下,分别提高12%~26%的目标油藏的采收率。

关键词：稠油降黏剂乳化微生物物理模拟驱油

猪源生物被膜乳酸菌的筛选与体外益生特性评价

在线全文

学校读者我要写书评

暂无评论

中国兽医科学 2019年第10期49卷 1288-1296页

作者：靳胜男舒慧萍张冬星赵泽林康元环张蕾单晓枫钱爱东吉林农业大学动物科学技术学院

为了研制优良的猪用微生态制剂,从健康猪的新鲜粪便中成功分离获得1株抑菌效果较好、生物被膜形成能力较强的L-11乳酸菌,经16S rDNA分子生物学鉴定,确定该分离株为约氏乳杆菌。并进一步对其体外益生特性包括产酸能力,耐酸碱、耐胰蛋白... 详细信息

为了研制优良的猪用微生态制剂,从健康猪的新鲜粪便中成功分离获得1株抑菌效果较好、生物被膜形成能力较强的L-11乳酸菌,经16S rDNA分子生物学鉴定,确定该分离株为约氏乳杆菌。并进一步对其体外益生特性包括产酸能力,耐酸碱、耐胰蛋白酶、耐高温、生长曲线、抑菌性能、黏附性以及对小鼠的致病性进行了检测。结果表明,该菌株具有一定的产酸能力,对pH4.0~10.0、10 g/L胰蛋白酶、50和60℃高温环境具有耐受能力;并在MRS液体培养基中培养大约4 h后进入对数生长期,12 h后达到稳定期;此外,该乳酸菌分离株具有广泛的抑菌谱和一定的黏附性,安全、无毒性。这说明乳酸菌L-11分离株可作为猪用益生菌研制的菌株。

关键词：乳酸菌生物被膜益生特性

Discovery of Kernel Row Number2 that enhances grain yield and its application in maize breeding

在线全文

学校读者我要写书评

暂无评论

Discovery of Kernel Row Number2 that enhances grain yield an...

山西省地下水2016-2020年水质变化趋势及污染因子分析

第二十届中国作物学会学术年会

作者：陈文康 Xuan Zhang Jianghua Guo Zhenyuan Chen Xiangyu zhao Xiuyi Fu Min Wang Shenghui Ji Pengfei Yin ning Yang Lu Chen Lichun Cai Jing Xu Lili Zhang Yingjia Han Yingni xiao Gen Xu Yuebin Wang Shuhui Wang Fang Yang David Jackson Jinkui Cheng Feng Qin Feng Tian Jianbing Yan Jiansheng Li 杨小红 State Key Laboratory of Plant Environmental Resilience and National Maize Improvement Center of China China Agricultural University National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Hubei Hongshan Laboratory Cold Spring Harbor Laboratory Cold Spring Harbor Center for Crop Functional Genomics and Molecular Breeding China Agricultural University

【Objective】 Increasing maize yield per unit area plays an important role in food demands and security. As a key component of maize yield, kernel row number(KRN) has a direct effect on improving maize yield per unit area. Therefore, understanding the genetic basis of KRN and cloning the gene controlling KRN are of great significance for molecular breeding of new maize varieties with high yield. 【Method】Following a recombinantderived progeny testing strategy, we performed positional cloning of q KRN2 for KRN identified in a maize recombinant inbred line(RIL) population developed from a cross between an inbred line, B73, and an introgression line, MT-6, of which ～25% of its genome is derived from that of teosinte, the wild ancestor of maize. 【Result】We delimited qKRN2 to a 5799–base pair(bp) region that contained only one candidate gene, which we named KRN2(Kernel Row Number2). Selection in the noncoding upstream regions resulted in a reduction of KRN2 expression and an increased grain number through an increase in kernel rows. KRN2 encodes a WD40 protein and function synergistically with a gene of unknown function, DUF1644, by protein-protein interaction. Field tests show that knockout of KRN2 in maize increased grain yield by ～10%, with no apparent trade-off in other agronomic traits. To further test whether KRN2 has the same effects in the elite inbred lines and hybrids used in modern maize breeding, we used two strategies—CRISPR/Cas9-mediated gene editing and marker assisted selection(MAS)—to improve a set of maize elite inbred lines. Compared with the corresponding wild-types, most of the improved lines with null allele of KRN2 showed an increased KRN by ～0.8 to 2.1 rows. Subsequently, we developed two improved hybrids, Zhengdan958 and Nongda788, for yield testing and investigated their KRN and other agronomic traits. Remarkably, both improved Zhengdan958 and improved Nongda788 derived from crossing improved male parent and female parent could increase KRN by ～1.5

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

环境科学导刊 2022年第6期41卷 73-79页

作者：孙晓杰赵立邢益毓郝晓丹刘红陈一宁太原师范学院山西晋中030619

2016-2020年,通过调查和研究山西省1139个地下水监测点位的水质,获取各点位水质超标因子及超标倍数、水质类别评价结果等,以国标为基准,采用地下水质量单项组分评价法和地下水质量综合评价法对山西省地下水进行水质分析和评价。研究结... 详细信息

2016-2020年,通过调查和研究山西省1139个地下水监测点位的水质,获取各点位水质超标因子及超标倍数、水质类别评价结果等,以国标为基准,采用地下水质量单项组分评价法和地下水质量综合评价法对山西省地下水进行水质分析和评价。研究结果表明:各监测点位中I~III类水比例<75%,V类水比例<20%,山西省总体地下水质属于轻度污染状态;山西省地下水污染主要发生在浅层孔隙水,中深层的岩溶水和裂隙水受到的影响较小;氟化物对山西省的地下水质有较大影响,随时间变化氨氮对地下水质的影响逐渐下降,硫酸根离子对山西省水质的影响由南到北呈递减趋势,氯离子是造成孔隙水污染的主要因子,总硬度、溶解性总固体和硫酸根离子对岩溶水污染的影响较大。

关键词：地下水水质评价变化趋势污染因子山西省

Expert Consensus on Diagnosis and Treatment of End-Stage Liver Disease Complicated with Infections

在线全文

学校读者我要写书评

暂无评论

Infectious Diseases & Immunity 2022年第3期2卷 168-178页

作者： Qin ning Tao Chen Guiqiang Wang Dong Xu Yanyan Yu Qing Mao Taisheng Li Lanjuan Li Jun Li xiaoju Lu Jiabin Li Zhiwei Li Wenhong Zhang Yonghong xiao Qinghua Meng Yuqiang Mi Jia Shang Yunsong Yu Yingren zhao Caiyan zhao Hong zhao Jianrong huang Jie Peng Hong Tang xiaoping Tang Jinhua Hu Bijie Hu Wei Guo Bo Zheng Baiyi Chen Yuexin Zhang Jia Wei Jifang Sheng Zhi Chen Minggui Wang Qing Xie Yuming Wang Fu-Sheng Wang Jinlin Hou Zhongping Duan Lai Wei Jidong Jia Chinese Society of Infectious Disease of Chinese Medical Association Department of Infectious Disease Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Infectious Disease Center for Liver DiseasePeking University First HospitalBeijing 100034China Department of Infectious Diseases Southwest HospitalArmy Medical UniversityChongqing 400038China Department of Infectious Diseases Peking Union Medical College HospitalPeking Union Medical College and Chinese Academy of Medical SciencesBeijing 100730China State Key Laboratory for Diagnosis and Treatment of Infectious Diseases Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesThe First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310003China Department of Infectious Disease The First Affiliated Hospital with Nanjing Medical UniversityNanjing 210029China Center of Infectious Diseases West China Hospital of Sichuan UniversityChengdu 610041China Department of Infectious Diseases First Affiliated Hospital of Anhui Medical UniversityHefei 230031China Department of Infectious Diseases Shengjing HospitalAffiliated Hospital of China Medical UniversityShenyang 110801China Department of Infectious Diseases Institute of Infectious DiseasesHuashan HospitalFudan UniversityShanghai 200040China Department of Severe Liver Diseases Beijing Youan HospitalCapital Medical UniversityBeijing 100069China Nankai University Second People’s Hospital Tianjin 300071China Department of Infectious Disease People’s Hospital of Henan ProvinceZhengzhou 450003China Department of Infectious Diseases Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310020China Department of Infectious Diseases First Affiliated Hospital of Xi’an Jiaotong UniversityXi’an 710061China Department of Infectious Diseases Third Affiliated Hospital of Hebei Medical UniversityShijiazhuang 050051China State Key Laboratory of Organ Failure Research Guangdong Provincial Key Laboratory of Viral Hepatitis ResearchDepartment o

End-stage liver disease(ESLD)is a life-threatening clinical syndrome that markedly increases mortality in patients with *** patients with ESLD,infections can induce or aggravate the occurrence of liver ***,infections are among the most common complications of disease *** is a lack of working procedure for early diagnosis and appropriate management for patients with ESLD complicated by infections as well as local and international guidelines or *** consensus assembled up-to-date knowledge and experience across Chinese colleagues,providing data on principles as well as working procedures for the diagnosis and treatment of patients with ESLD complicated by infections.

关键词： Consensus Diagnosis End stage of liver disease Infection Therapy