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An inducible CRISPR activation tool for accelerating plant regeneration

Plant Communications 2024年第5期5卷 109-122页

作者： Cuimei Zhang Yajun Tang Shanjie Tang Lei Chen Tong Li Haidi Yuan Yujun Xu Yangyan Zhou Shuaibin Zhang Jianli Wang Hongyu Wen Wenbo Jiang Yongzhen Pang Xian Deng Xiaofeng Cao Junhui Zhou xianwei song Qikun Liu State Key Laboratory of Protein and Plant Gene Research School of Advanced Agricultural SciencesPeking UniversityBeijing 100871China Peking University Institute of Advanced Agricultural Sciences Shandong Laboratory of Advanced Agricultural Sciences at WeifangShandong 261000China National Center for Plant Gene Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Grass and Science Institute of Heilongjiang Academy of Agricultural Sciences Heilongjiang 150086China Institute of Animal Science Chinese Academy of Agricultural SciencesBeijing 100193China Key Laboratory of Seed Innovation and National Center for Plant Gene Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China

The inducible CRISPR activation(CRISPR-a)system offers unparalleled precision and versatility for regu-lating endogenous genes,making it highly sought after in plant *** this study,we developed a chem-ically inducible CRISPR-a tool for plants called ER-Tag by combining the LexA-VP16-ER inducible system with the SunTag CRISPR-a *** systematically compared different induction strategies and achieved high efficiency in target gene *** demonstrated that guide RNAs can be multiplexed and pooled for large-scale screening of effective morphogenic genes and gene pairs involved in plant *** experiments showed that induced activation of these morphogenic genes can accelerate regenera-tion and improve regeneration efficiency in both eudicot and monocot plants,including alfalfa,woodland strawberry,and *** study expands the CRISPR toolset in plants and provides a powerful new strategy for studying gene function when constitutive expression is not feasible or ideal.

关键词： inducible CRISPR activation morphogenic gene genetic transformation plant regeneration

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“直接电影”美学视阈下纪录片创作的共生关系——以纪录片《美国工厂》《蜂蜜之地》为例

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四川戏剧 2024年第8期 98-103,111页

作者：宋献伟山西传媒学院视听学院

富于“直接电影”美学特征的纪录片《美国工厂》《蜂蜜之地》获得国际电影奖项,使得“直接电影”手法再次引起纪录片界的关注,纪录片创作中拍摄者与被摄者的共生关系也被细致探讨。回顾“直接电影”的美学嬗变过程可以发现,拍摄者与被... 详细信息

富于“直接电影”美学特征的纪录片《美国工厂》《蜂蜜之地》获得国际电影奖项,使得“直接电影”手法再次引起纪录片界的关注,纪录片创作中拍摄者与被摄者的共生关系也被细致探讨。回顾“直接电影”的美学嬗变过程可以发现,拍摄者与被摄者的共生关系不是简单的纪录片伦理关系,而是包含了纪实美学、叙事美学、视听美学、接受美学在内的多维度的共生关系,集中体现在拍摄者与被摄者共生关系的叙事呈现范畴内不同动机诉求下的故事共生、不同角度静观下的情感共生、不同立场建构下的认知共生等方面。基于此,纪录片创作者如何运用“直接电影”的美学理念指导创作,处理好叙事美学角度的预设议程与随机调整、视听美学角度的独立品格与合谋呈现、接受美学角度的人性揭露与人文关怀等具体问题,值得深入研究。

关键词： “直接电影” 《美国工厂》《蜂蜜之地》纪录片创作共生关系

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Telomere-to-telomere genome of the allotetraploid legume Sesbania cannabina reveals transposon-driven subgenome divergence and mechanisms of alkaline stress tolerance

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Science China(Life Sciences) 2024年第1期67卷 149-160页

作者： Haofei Luo Xiaofei Wang Changqing You Xuedan Wu Duofeng Pan Zhiyao Lv Tong Li Dongmei Zhang Zhongbao Shen Xiaodong Zhang Guodao Liu Kaixuan He Qingtong Ye Yajun Jia Qinghua Zhao Xian Deng Xiaofeng Cao xianwei song Gai Huang State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Hainan Yazhou Bay Seed Lab Sanya 572025China University of Chinese Academy of Sciences Beijing 100049China Institute of Forage and Grassland Sciences Heilongjiang Academy of Agricultural SciencesHarbin 150086China Shandong Academy of Agricultural Sciences Jinan 250100China National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land Dongying 257345China Shandong Green Manure Ecological Technology Co. LtdDongying 257345China State Key Laboratory of Tropical Crop Breeding Chinese Academy of Tropical Agricultural SciencesHaikou 571101China

Alkaline soils pose an increasing problem for agriculture worldwide,but using stress-tolerant plants as green manure can improve marginal ***,we show that the legume Sesbania cannabina is very tolerant to alkaline conditions and,when used as a green manure,substantially improves alkaline *** understand genome evolution and the mechanisms of stress tolerance in this allotetraploid legume,we generated the first telomere-to-telomere genome assembly of *** spanning~2,087 *** assembly included all centromeric regions,which contain centromeric satellite repeats,and complete chromosome ends with telomeric *** genome analysis distinguished A and B subgenomes,which diverged approximately 7.9 million years *** genomic analysis revealed that the chromosome homoeologs underwent large-scale inversion events(>10 Mb)and a significant,transposon-driven size expansion of the chromosome 5A *** further identified four specific alkali-induced phosphate transporter genes in ***;these may function in alkali tolerance by relieving the deficiency in available phosphorus in alkaline *** work highlights the significance of *** as a green tool to improve marginal lands and sheds light on subgenome evolution and adaptation to alkaline soils.

关键词： Sesbania cannabina alkaline stress telomere-to-telomere genome legume green manure phosphate transporter allotetraploid

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Grey-theory based intrusion detection model

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Journal of Systems Engineering and Electronics 2006年第1期17卷 230-235页

作者： Qin Boping Zhou xianwei Yang Jun song Cunyi School of Information Engineering Univ. of Science and Technology Beijing Beijing 100083 P. R. China

To solve the problem that current intrusion detection model needs large-scale data in formulating the model in real-time use, an intrusion detection system model based on grey theory （GTIDS） is presented. Grey theory has merits of fewer requirements on original data scale, less limitation of the distribution pattern and simpler algorithm in modeling. With these merits GTIDS constructs model according to partial time sequence for rapid detect on intrusive act in secure system. In this detection model rate of false drop and false retrieval are effectively reduced through twice modeling and repeated detect on target data. Furthermore, GTIDS framework and specific process of modeling algorithm are presented. The affectivity of GTIDS is proved through emulated experiments comparing snort and next-generation intrusion detection expert system （NIDES） in SRI international.

关键词： network security intrusion detection grey theory model.

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北部湾防城河流域水源涵养格局及其对降雨的响应特征

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应用生态学报 2024年第2期35卷 407-414页

作者：甘文静莫上萱张建洪宋贤威冼金梅杨露农海勤广西大学林学院广西森林生态与保育重点实验室广西高校亚热带人工林培育与利用重点实验室南宁530004 广西壮族自治区水文中心南宁530023

评估南亚热带季风气候影响下流域水源涵养量的时空格局及其对降雨的响应特征,可为揭示不同时间尺度流域降水变化下水源涵养演变规律提供科学依据。本研究以北部湾防城河流域为研究对象,基于水量平衡原理,应用SWAT模型探究水源涵养量的... 详细信息

评估南亚热带季风气候影响下流域水源涵养量的时空格局及其对降雨的响应特征,可为揭示不同时间尺度流域降水变化下水源涵养演变规律提供科学依据。本研究以北部湾防城河流域为研究对象,基于水量平衡原理,应用SWAT模型探究水源涵养量的时空格局及其对降水的响应特征。结果表明:防城河流域水源涵养量为1637.4 mm·a^(-1),占年均降水量的50.7%。不同子流域的水源涵养变化量的差异明显,其中,森林植被覆盖度高、坡度较陡的子流域水源涵养量大,而其他土地利用类型(如田地、草地)、坡度较缓、人类活动强度大的子流域水源涵养量较低。在月尺度上,水源涵养量和水源涵养变化量均与流域降水呈现相似的变化特征。水源涵养变化量对次降水量的响应呈现两种类型:短期降雨(降雨持续时长≤2 d)与水源涵养变化量呈线性变化,中长期降雨(2 d<降雨持续时长≤10 d)与水源涵养变化量呈非线性曲线变化,且主要受到蒸散发等过程的影响。高频次短持续时间的降水事件相对于长持续时间的降水事件更有利于增加生态系统水源涵养。

关键词：水源涵养 SWAT模型时空格局降水水文模拟

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Epigenetic regulation and epigenomic landscape in rice

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National Science Review 2016年第3期3卷 309-327页

作者： Xian Deng xianwei song Liya Wei Chunyan Liu Xiaofeng Cao State Key Laboratory of Plant Genomics and National Center for Plant Gene Research CAS Center for Excellence in Molecular Plant SciencesInstitute of Genetics and Developmental BiologyChinese Academy of Sciences College of Life Sciences Hebei University

Epigenetic regulation has been implicated in the control of complex agronomic traits in rice(Oryza sativa),a staple food crop and model monocot plant. Recent advances in high-throughput sequencing and the moderately complex genome of rice have made it possible to study epigenetic regulation in rice on a genome-wide scale. This review discusses recent advances in our understanding of epigenetic regulation in rice, with an emphasis on the roles of key epigenetic regulators, the epigenomic landscape, epigenetic variation, transposon repression, and plant development.

关键词： epigenetics DNA methylation histone modification non-coding RNAs transposable element epigenomics rice

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Targeted DNA demethylation produces heritable epialleles in rice

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Science China(Life Sciences) 2022年第4期65卷 753-756页

作者： Shanjie Tang Chao Yang Dong Wang Xian Deng Xiaofeng Cao xianwei song State Key Laboratory of Plant Genomics and National Center for Plant Gene Research(Beijing) Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing100101China University of Chinese Academy of Sciences Beijing100049China Key Laboratory of Molecular Biology and Gene Engineering in Jiangxi Province College of Life ScienceNanchang UniversityNanchang330031China Center for Excellence in Molecular Plant Sciences Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing100101China Innovative Academy of Seed Design Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing100101China

Dear Editor,DNA methylation is an important epigenetic mark that is associated with the silencing of genes and transposable elements(TEs)(Law and Jacobsen,2010).Changes in DNA methylation can be transgenerationally in... 详细信息

关键词： alleles rational Targeted

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喀斯特地下河涌出后CO_(2)逸散通量的时空格局——基于广西巴马盘阳河

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中国岩溶 2024年第4期43卷 753-765页

作者：冼金梅宋贤威张乐林沛新韦岳春杨取宝甘文静杨露广西大学林学院广西南宁530000 广西巴马和泰长寿产业有限公司广西河池市巴马瑶族自治县547503 广西河池市巴马瑶族自治县气象局广西河池547503

喀斯特流域是岩石风化碳汇的关键区域,同时也是CO_(2)逸散研究的热点区域。为探究喀斯特地下河涌出后CO_(2)分压(pCO_(2))及其逸散通量的时空变化格局,选择喀斯特流域巴马盘阳河为对象,分析水体的pH、碱度、总溶解性固体(TDS)、溶解无机... 详细信息

喀斯特流域是岩石风化碳汇的关键区域,同时也是CO_(2)逸散研究的热点区域。为探究喀斯特地下河涌出后CO_(2)分压(pCO_(2))及其逸散通量的时空变化格局,选择喀斯特流域巴马盘阳河为对象,分析水体的pH、碱度、总溶解性固体(TDS)、溶解无机碳(DIC)、溶解有机碳(DOC)、pCO_(2)的时空变化特征,探讨pCO_(2)的调控因素并估算了CO_(2)逸散通量。结果表明,流域内地下水碱度、TDS、DIC和pCO_(2)显著高于地表水,表明喀斯特碳酸盐风化释放大量DIC进入地下水,地下水涌出后产生CO_(2)逸散降低了地表水DIC含量和pCO_(2)。在时间尺度上,旱季常规地表、地下水的碱度、TDS、DIC、pCO_(2)、CO_(2)逸散通量均显著高于雨季,主要归结于雨季雨水的稀释效应。然而次降雨事件下地表、地下水的pH、碱度、TDS、DIC、DOC、pCO_(2)无显著性差异,可能由于降雨量不足或降雨持续时间短。研究期间,巴马盘阳河流域地表水、地下水CO_(2)逸散通量范围分别为-0.10~9.20 kg C m^(-2)year^(-1),-0.12~17.28kg C m^(-2)year^(-1),平均CO_(2)逸散通量分别为1.06±1.46 kg C m^(-2)year^(-1)和2.40±3.14 kg C m^(-2)year^(-1),远高于全球主要大型流域的平均CO_(2)逸散通量(0.64 kg Cm^(-2)year^(-1))。阐明喀斯特流域的CO_(2)逸散通量及其时空变化特征对准确评估河流碳收支状况与评估岩石风化碳汇具有重要意义。

关键词：喀斯特岩溶泉溶解无机碳河流CO_(2)分压 CO_(2)逸散通量

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Mobile ARGONAUTE 1d binds 22-nt miRNAs to generate phasiRNAs important for low-temperature male fertility in rice

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Science China(Life Sciences) 2023年第2期66卷 197-208页

作者： Fuyan Si Haofei Luo Chao Yang Jie Gong Bin Yan Chunyan Liu xianwei song Xiaofeng Cao State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China The Municipal Key Laboratory of the Molecular Genetics of Hybrid Wheat Institute of Hybrid WheatBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China Innovative Academy of Seed Design(INASEED) Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China College of Life Sciences University of the Chinese Academy of SciencesBeijing 100039China CAS Center for Excellence in Molecular Plant Sciences Chinese Academy of SciencesBeijing 100101China

Phased small interfering RNAs(phasiRNAs) are abundantly expressed in anthers and linked to environment-related male fertility in grasses, yet how they function under different environmental conditions remains unclear. Here, we identified a rice(Oryza sativa) low temperature-induced Argonaute(AGO) protein, OsAGO1d, that is responsible for generating phasiRNAs and preserving male fertility at low temperature. Loss of OsAGO1d function causes low-temperature male sterility associated with delayed programmed cell death of tapetal cells during anther development. OsAGO1d binds miR2118 and miR2275 family members and triggers phasiRNA biogenesis;it also binds 21-nt phasiRNAs with a 5′ terminal U. In total, phasiRNAs from 972loci are OsAGO1d-dependent. OsAGO1d protein moves from anther wall cells into meiocytes, where it loads miR2275 to produce 24-nt phasiRNAs. Together, our results show that OsAGO1d acts as a mobile signal to fine-tune phasiRNA production and this function is important for male fertility at low temperature.

关键词： mobile signal ARGONAUTE 22-nt miRNA phasiRNA low temperature male sterility rice

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An epiallele of rice AK1 affects photosynthetic capacity

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Journal of Integrative Plant Biology 2017年第3期59卷 158-163页

作者： Xiangjin Wei xianwei song Liya Wei Shaoqing Tang Jing Sun Peisong Hu Xiaofeng Cao State Key Laboratory of Rice Biology Key Laboratory of Rice Biology and Breeding of Ministry of AgricultureChina National Rice Research InstituteHangzhou 310006China State Key Laboratory of Plant Genomics and National Center for Plant Gene Research CAS Center for Excellence in Molecular Plant SciencesInstitute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China College of Life Sciences Hebei UniversityBaoding O71OO2China

Summary Epigenetic gene variants, termed epialleles, can broaden genetic and phenotypic diversity in eukaryotes. Here, we identify a natural epiallele of OsAK1, which encodes a rice adenylate kinase. The Epiak1 plants... 详细信息

关键词： gene Figure AK An epiallele of rice AK1 affects photosynthetic capacity

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