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Grapevine VlbZIP30 improves drought resistance by directly activating VvNAC17 and promoting lignin biosynthesis through the regulation of three peroxidase genes

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Horticulture Research 2020年第1期7卷 1061-1075页

作者： Mingxing Tu xianhang wang Wuchen Yin Ya wang Yajuan Li Guofeng Zhang Zhi Li Junyang Song Xiping wang State Key Laboratory of Crop Stress Biology in Arid Areas College of HorticultureNorthwest A&F UniversityYangling 712100 ShaanxiChina Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China Ministry of AgricultureNorthwest A&F UniversityYangling 712100 ShaanxiChina College of Enology Northwest A&F UniversityYangling 712100 ShaanxiChina

Drought stress severely affects grapevine quality and yield,and recent reports have revealed that lignin plays an important role in protection from drought *** little is known about lignin-mediated drought resistance in grapevine,we investigated its ***,we show that VlbZIP30 mediates drought resistance by activating the expression of lignin biosynthetic genes and increasing lignin *** grapevine plants overexpressing VlbZIP30 exhibited lignin deposition(mainly G and S monomers)in the stem secondary xylem under control conditions,which resulted from the upregulated expression of VvPRX4 and *** of VlbZIP30 improves drought tolerance,characterized by a reduction in the water loss rate,maintenance of an effective photosynthesis rate,and increased lignin content(mainly G monomer)in leaves under drought *** mobility shift assay,luciferase reporter assays,and chromatin immunoprecipitation-qPCR assays indicated that VlbZIP30 directly binds to the G-box cis-element in the promoters of lignin biosynthetic(VvPRX N1)and drought-responsive(VvNAC17)genes to regulate their *** summary,we report a novel VlbZIP30-mediated mechanism linking lignification and drought tolerance in *** results of this study may be of value for the development of molecular breeding strategies to produce drought-resistant fruit crops.

关键词： directly breeding drought

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VlbZIP30 of grapevine functions in dehydration tolerance via the abscisic acid core signaling pathway

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Horticulture Research 2018年第1期5卷 308-322页

作者： Mingxing Tu xianhang wang Yanxun Zhu Dejun wang Xuechuan Zhang Ye Cui Yajuan Li Min Gao Zhi Li Yuejin wang Xiping wang State Key Laboratory of Crop Stress Biology in Arid Areas College of HorticultureNorthwest A&F University712100YanglingShaanxiChina Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China Ministry of AgricultureNorthwest A&F University712100YanglingShaanxiChina Biomarker Technologies Corporation 101300BeijingChina

Drought stress limits the growth and development of grapevines,thereby reducing productivity,but the mechanisms by which grapevines respond to drought stress remain largely ***,we characterized a group A bZIP gene from“Kyoho”grapevine,VlbZIP30,which was shown to be induced by abscisic acid(ABA)and dehydration *** of VlbZIP30 in transgenic Arabidopsis thaliana enhanced dehydration *** analysis revealed that a major proportion of ABA-responsive and/or drought-responsive genes are transcriptionally regulated by VlbZIP30 during ABA or mannitol treatment at the cotyledon greening *** identified an *** G-box motif(CACGTG)and a potential grapevine G-box motif(MCACGTGK)in the promoters of the 39 selected *** genes upregulated in the transgenic plants and in the 35 grapevine homologs,***,using two grapevine-related databases,we found that 74%(23/31)and 84%(21/25)of the detected grapevine genes were significantly upregulated by ABA and drought stress,respectively,suggesting that these genes are involved in ABA or dehydration stress and may be regulated by VlbZIP30 in *** propose that VlbZIP30 functions as a positive regulator of dehydration-responsive signaling in the ABA core signaling pathway.

关键词： ZIP drought functions

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Whole-genome sequencing reveals rare off-target mutations in CRISPR/Cas9-edited grapevine

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Horticulture Research 2021年第1期8卷 1352-1362页

作者： xianhang wang Mingxing Tu Ya wang Wuchen Yin Yu Zhang Hongsong Wu Yincong Gu Zhi Li Zhumei Xi Xiping wang State Key Laboratory of Crop Stress Biology in Arid Areas College of HorticultureNorthwest A&F University712100 YanglingShaanxiChina College of Enology Northwest A&F University712100 YanglingShaanxiChina Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China Ministry of AgricultureNorthwest A&F University712100 YanglingShaanxiChina Novogene Technologies Corporation 100000 BeijingChina OEbiotech Corporation 200000 ShanghaiChina

The CRISPR(clustered regularly interspaced short palindromic repeats)-associated protein 9(Cas9)system is a powerful tool for targeted genome editing,with applications that include plant biotechnology and functional genomics ***,the specificity of Cas9 targeting is poorly investigated in many plant species,including fruit *** assess the off-target mutation rate in grapevine(Vitis vinifera),we performed whole-genome sequencing(WGS)of seven Cas9-edited grapevine plants in which one of two genes was targeted by CRISPR/Cas9 and three wild-type(WT)*** total,we identified between 202,008 and 272,397 single nucleotide polymorphisms(SNPs)and between 26,391 and 55,414 insertions/deletions(indels)in the seven Cas9-edited grapevine plants compared with the three WT ***,3272 potential off-target sites were selected for further *** one off-target indel mutation was identified from the WGS data and validated by Sanger *** addition,we found 243 newly generated off-target sites caused by genetic variants between the Thompson Seedless cultivar and the grape reference genome(PN40024)but no true off-target *** conclusion,we observed high specificity of CRISPR/Cas9 for genome editing of grapevine.

关键词： Whole Sanger rare

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传统无机化学材料的应用现状及新材料的发展探索

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化工管理 2024年第35期 112-115页

作者：王先航天津城建大学理学院天津300384

无机化学材料作为工业基石之一,在医药、环保、新能源等领域的应用日益广泛。然而,传统材料的功能局限、稳定性不足、寿命短及环境污染等问题亟待解决。为此,文章聚焦于新材料开发,探索低价无机物质转化为高性能材料的路径。实验表明,... 详细信息

无机化学材料作为工业基石之一,在医药、环保、新能源等领域的应用日益广泛。然而,传统材料的功能局限、稳定性不足、寿命短及环境污染等问题亟待解决。为此,文章聚焦于新材料开发,探索低价无机物质转化为高性能材料的路径。实验表明,新材料在机械强度、电气特性及环保性上均有显著优势,不仅拓宽了无机材料的应用领域,也为提升材料性能、优化生产过程、降低环境污染提供了新策略,对推动社会科技进步具有重要意义。

关键词：传统无机化学材料新材料开发环境保护

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肝癌细胞侵袭行为与其流变特性相关

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生物医学工程学杂志 2000年第2期17卷 133-138页

作者：吴泽志罗向东王宪航张钢龙勉蔡绍皙重庆大学生物工程学院第三军医大学烧伤研究所重庆400038

以原代肝细胞、肝细胞癌（Hepatocellularcarcinoma，HCC）细胞及对人工基底膜Matrigel具有侵袭能力的HCC细胞（HCC-Inv细胞）为研究对象，研究了三种细胞的粘弹特性、与胶原蛋白Ⅰ的粘附力学特性及与肝血窦内皮细胞（Liversinosoidalen... 详细信息

以原代肝细胞、肝细胞癌（Hepatocellularcarcinoma，HCC）细胞及对人工基底膜Matrigel具有侵袭能力的HCC细胞（HCC-Inv细胞）为研究对象，研究了三种细胞的粘弹特性、与胶原蛋白Ⅰ的粘附力学特性及与肝血窦内皮细胞（Liversinosoidalendothelialcell，LEC）的粘附力学特性。结果发现，HCC细胞的上述流变特性较之于肝细胞发生明显的变化；与肝细胞及HCC细胞相比较，HCC－Inv细胞表现出高的粘弹特性，高的与胶原蛋白Ⅰ的粘附力，高的与LEC的粘附力；免疫细胞化学的结果证实了HCC细胞与HCC－Inv细胞形态与细胞骨架结构的差异。作者对肝癌细胞流变特性改变及病理学意义作了简要讨论。

关键词：肝细胞癌胶原蛋白Ⅰ Matrigel 细胞流变学

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肝癌细胞与胶原蛋白Ⅰ裱衬表面粘附特性

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生物物理学报 1996年第4期12卷 686-690页

作者：王宪航龙勉王晓军王红兵罗向东吴泽志宋关斌吴云鹏重庆大学生物工程研究院第三军医大学烧伤研究所

本文以正常胎肝细胞的原代培养及肝癌细胞的传代培养为基础，应用微管吸吮技术在单个细胞水平上研究肝实质细胞癌细胞在胶原蛋白Ⅰ裱衬表面的粘附力学特性。实验将正常原代肝细胞与肝癌细胞进行对照，阐明肝癌细胞与胶原蛋白Ⅰ裱衬表面... 详细信息

本文以正常胎肝细胞的原代培养及肝癌细胞的传代培养为基础，应用微管吸吮技术在单个细胞水平上研究肝实质细胞癌细胞在胶原蛋白Ⅰ裱衬表面的粘附力学特性。实验将正常原代肝细胞与肝癌细胞进行对照，阐明肝癌细胞与胶原蛋白Ⅰ裱衬表面粘附行为的时间与浓度依赖性，结果表明肝癌细胞与胶原蛋白Ⅰ裱衬表面具有更强的粘附力。这一研究对肝癌转移的定量研究有方法学的参考意义。

关键词：肝癌细胞胶原蛋白Ⅰ 粘附特性

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二苯基乙二胺衍生的铜配合物催化丙交酯聚合

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广州化学 2023年第4期48卷 53-56页

作者：赵宁屈晨阳刘浩哲汪显杭杨正超陕西科技大学化学与化工学院陕西西安710021 中国石油独山子石化公司新疆克拉玛依833699

用(1R,2R)-二苯基乙二胺和水杨醛为原料合成了具有C_(2)对称性的手性配体1,将1与Cu(OAc)_(2)反应制备了手性铜配合物2,并用其来催化外消旋丙交酯(rac-LA)聚合。经核磁共振、IR、元素分析和X-单晶衍射确定配体1和配合物2的结构,经TGA确... 详细信息

用(1R,2R)-二苯基乙二胺和水杨醛为原料合成了具有C_(2)对称性的手性配体1,将1与Cu(OAc)_(2)反应制备了手性铜配合物2,并用其来催化外消旋丙交酯(rac-LA)聚合。经核磁共振、IR、元素分析和X-单晶衍射确定配体1和配合物2的结构,经TGA确定了配合物2的热稳定性,通过GPC确定了聚合物的分子量和分子量分布。结果表明,配合物2在聚合反应温度下的结构是稳定的,能有效催化rac-LA进行本体聚合,所得聚合物分子量分布较窄,但分子量较低,这可能是由于高温下发生了酯交换反应,同时发现异丙醇的加入使聚合物的分子量明显增大。

关键词：铜配合物丙交酯聚合催化聚乳酸结构

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Microseismic event waveform classification using CNN-based transfer learning models

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International Journal of Mining Science and Technology 2023年第10期33卷 1203-1216页

作者： Longjun Dong Hongmei Shu Zheng Tang xianhang Yan School of Resources and Safety Engineering Central South UniversityChangsha 410083China International College of Digital Innovation Chiang Mai UniversityChiang Mai 50200Thailand

The efficient processing of large amounts of data collected by the microseismic monitoring system(MMS),especially the rapid identification of microseismic events in explosions and noise,is essential for mine disaster ***,this work is primarily performed by skilled technicians,which results in severe workloads and *** this paper,CNN-based transfer learning combined with computer vision technology was used to achieve automatic recognition and classification of multichannel microseismic signal ***,data collected by MMS was generated into 6-channel original waveforms based on *** that,sample data sets of microseismic events,blasts,drillings,and noises were established through manual *** datasets were split into training sets and test sets according to a certain proportion,and transfer learning was performed on AlexNet,GoogLeNet,and ResNet50 pre-training network models,*** training and tuning,optimal models were retained and compared with support vector machine *** show that transfer learning models perform well on different test ***,GoogLeNet performed best,with a recognition accuracy of 99.8%.Finally,the possible effects of the number of training sets and the imbalance of different types of sample data on the accuracy and effectiveness of classification models were discussed.

关键词： Mine safety Machine learning Transfer learning Microseismic events Waveform classification Image identification and classification

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Thermogravimetric study of the effect of a PVA oxygen-insulating barrier on the spontaneous combustion of coal

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Mining Science and Technology 2010年第6期20卷 882-885页

作者： MENG xianhang CHU Ruizhi WU Guoguang XU Hongfeng ZHU Jiamei wang Zhihua School of Chemical Engineering and Technology China University of Mining & Technology Xuzhou 221008 China Key Laboratory of Coal Processing and Efficient Utilization China University of Mining & Technology Ministry of Education Xuzhou 221008 China

Coal samples in the air for three months were characterized by Thermogravimetric Analysis (TGA). The effect of a PVA oxygen-insulating barrier on the spontaneous combustion of coal was examined. The moisture loss activation energy, oxidation activation energy and combustion activation energy were calculated by an integral method using the Coats-Redfen formula. The results show that the tendency for spontaneous combustion of three coal samples (judged by the activation energy) falls in the order: CYW>YJL>SW. The oxidation activation energy and combustion activation energy of coal protected by the PVA oxygen-insulating barrier increased. A significant increase in the combustion activation energy was noted, especially for the CYW coal where the in-crease was 28.53 kJ/mol. Hence, oxidation of the protected coal samples was more difficult. The PVA oxygen-insulating barrier helps to prevent spontaneous combustion of the coal.

关键词： coal spontaneous combustion oxygen-insulating barrier activation energy thermogravimetric analysis

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Research on the Path and Guarantee Mechanism of the Integration of Industry and Educationfor Applied Undergraduates--A Case Study of Human Resource Management Major

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教育研究前沿（中英文版） 2021年第3期11卷 46-51页

作者： xianhang Xu School of Innovation and Entrepreneurship Chongqing Institute of EngineeringChongqing 400056China

Deepening the integration of industryand educationand school-enterprise cooperation are effective waysto cultivate high-quality applied *** has become an essentialdemand for social and economic development and an inevitable trend for applied *** view of this,based on the development strategy of national and local integration of industry and education and the demand for talents in the human resources industry,this article takes human resource management as an example,combined with the current situation of industry and education integration of applied undergraduate human resource management,and explores its products from the perspective of one course and two *** also proposes a guarantee mechanism for integratingindustryand education from the aspects of top-level guarantee,multiple evaluations,teaching co-management,and teacher *** is hoped to establishan application-oriented undergraduate human resource management professional industryand education implementation systemand promote the deep integration of applied undergraduate industry and education.

关键词： Applied Undergraduate Integration of Industry and Education One Course with Two Teachers

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