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奥奈达希瓦氏菌(Shewanella oneidensis)MR-1对氟西汀降解过程的转录组及功能基因分析

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生态与农村环境学报 2024年第4期40卷 532-540页

作者：万宇杭小帅王志刚张澜周莉尤晓慧朱冬冬王燕肖静陈翔扬州大学环境科学与工程学院江苏扬州225009 生态环境部南京环境科学研究所江苏南京210042

该文研究了厌氧条件下奥奈达希瓦氏菌(Shewanella oneidensis)MR-1对氟西汀的降解性能,并从转录组学分析遗传分子代谢机制。结果表明,Shewanella oneidensis MR-1在厌氧条件下可有效降解体系中93.12%的氟西汀,降解速率达0.94 mg·L^(-1)... 详细信息

该文研究了厌氧条件下奥奈达希瓦氏菌(Shewanella oneidensis)MR-1对氟西汀的降解性能,并从转录组学分析遗传分子代谢机制。结果表明,Shewanella oneidensis MR-1在厌氧条件下可有效降解体系中93.12%的氟西汀,降解速率达0.94 mg·L^(-1)·h^(-1)。采用Illumina高通量测序平台对降解氟西汀后的细菌与对照组细菌进行测序,通过基因本体数据库(GO)和京都基因与基因组百科全书数据库(KEGG)富集分析,结合筛选的高表达高上调的差异表达基因,得到了耐受和降解氟西汀的功能基因,其中包膜应激反应膜蛋白基因、ABC转运蛋白基因、噬菌体休克蛋白PspA基因、氧化应激防御蛋白基因等在Shewanella oneidensis MR-1对环境的耐受中起重要作用;细胞色素C基因、硝基还原酶NfsB基因、乳酸利用蛋白基因等在氟西汀的降解转化中起关键作用。该研究从转录组水平分析了氟西汀的降解机制,可为Shewanella oneidensis MR-1在环境修复中的应用提供参考依据。

关键词： Shewanella oneidensis MR-1 氟西汀转录组差异表达基因功能基因

Identification and characterization of cell cultures with various embryogenic/regenerative potential in cotton based on morphological,cytochemical,and cytogenetical assessment

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Journal of Integrative Agriculture 2019年第1期18卷 1-8页

作者： GUO hui-hui WU Jian-fei CHEN Cui-xia WANG Hong-mei zhao Yun-lei ZHANG Chao-jun JIA Yin-hua LIU Fang NING Tang-yuan CHU zhao-hui ZENG Fan-chang State Key Laboratory of Crop Biology Ministry of Science and Technology/College of AgronomyShandong Agricultural University State Key Laboratory of Cotton Biology Ministry of Science and Technology/Cotton Research InstituteChinese Academy of Agricultural Sciences Key Laboratory of Water Resources and Drought Resistant Germplasm Improvement Ministry of Agriculture

Somatic embryogenesis(SE) plays a vital role in genetic transformation and massive propagation of important agronomical and economical ***,we conducted a systematic assessment of the morphological,cytochemical,and cytogenetical characteristics of six culture strains with various embryogenic/regenerative potential during SE process in *** indicated that the six cell culture strains had stable ploidy levels,and did not reveal any relationship between the cytogenetic state and their morphogenetic ***,the six culture strains were compared via double staining with Evans blue and Acetocarmine to efficiently distinguish embryogenic and non-embryogenic cells and determine the embryogenic nature of the *** addition,the kind of auxins added in medium affected not only growth property,color,size of cell clumps but also ploidy level and regeneration *** combining analysis of morphological,cytochemical,and cytogenetical characteristics of the cell cultures,we are able to obtain and maintain homogeneous cell population with high morphogenic and regeneration ability and establish efficient somatic embryogenesis and regeneration system from short-term cell cultures in upland cotton,which highlight the application of biotechnological approaches in crop breeding,and above all,to better understand totipotency of cells in higher plants.

关键词： cotton somatic embryogenesis cotton cell culture strains cytochemical EB-AC double staining cytogenetical ploidy stability embryogenic/regenerative potential, morphology

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用户侧电力碳排放量预测方法

计算机仿真 2024年第9期41卷 494-499页

作者：张禄严嘉慧王立永李香龙国网北京市电力公司电力科学研究院

为了帮助电力行业分摊碳排放责任，优化碳减排策略，研究了用户侧电力相关碳排放预测方法。按照先预测用电量，再根据电碳转换换算的方法实现用户侧电力相关碳排放的预测。其中，用户的用电量预测是核心。设计一种多模态嵌入的循环神经... 详细信息

为了帮助电力行业分摊碳排放责任，优化碳减排策略，研究了用户侧电力相关碳排放预测方法。按照先预测用电量，再根据电碳转换换算的方法实现用户侧电力相关碳排放的预测。其中，用户的用电量预测是核心。设计一种多模态嵌入的循环神经网络，考虑用电量的多重影响因素，建模用电序列的短期依赖关系；提出一种历史注意力机制，考虑用户用电习惯的周期性特点，捕获用电序列中的周期性因素。实验结果表明，上述方法的用电量预测结果性能明显地优于一些常用的用电量预测方法，有助于用户侧电力相关碳排放的准确预测。

关键词：用户侧碳排放用电量预测循环神经网络注意力机制周期性

Density disturbance of small-scale fieldaligned irregularities in the ionosphere heating experiments

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Plasma Science and Technology 2018年第12期20卷 9-18页

作者： xiang WANG Chen ZHOU Moran LIU Binbin NI Zhengyu zhao Department of Space Physics School of Electronic InformationWuhan UniversityWuhan 430072People＇s Republic of China

A theoretical model which describes the small-scale irregularities excited by powerful high frequency （3–30 MHz） electromagnetic wave in ionosphere heating is investigated quantitatively in this paper. The model is based on the transport equation in magnetic plasma and mode conversion from electromagnetic wave to electrostatic wave in ionospheric *** electric field for exciting small-scale （meter scale） irregularities and spatial spectra of irregularities are analytically calculated by this model. The results indicate that background electron density and geomagnetic field play an important role for the threshold electric field and the spatial scale of the electron density irregularities. The results demonstrate that the electric field threshold increases with the decrease of the spatial scale of the irregularities. For exciting meter scale irregularities, the threshold electric field is about tens of mV m^（-1）. The theoretical results are consistent with those of the experiments.

关键词： small-scale irregularities ionosphere heating experiment thermal parametric instability resonant instability

饲粮中添加活性酵母对生长肉兔生长性能、肉品质和肠道菌群的影响

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动物营养学报 2024年第4期36卷 2603-2611页

作者：李响谢乾成郭文文毛晓峰宋光明刘永需赵红李福昌刘磊山东农业大学动物科技学院农业农村部非粮食饲料资源高效利用重点实验室(部省共建)山东省动物生物技术与疾病预防控制重点实验室泰安271018 邹平市农业农村局滨州256200 青岛峰尚生物技术有限公司青岛266000 乐斯福管理(上海)有限公司上海200235 青岛康大佳惠饲料有限公司青岛266499

本试验旨在研究饲粮中添加活性酵母对生长肉兔生长性能、肉品质和肠道菌群的影响。选取体重相近的健康35日龄断奶仔兔360只,随机分为3组,每组6个重复,每个重复20只仔兔。对照组饲喂基础饲粮(不添加活性酵母),试验组分别在基础饲粮中添加... 详细信息

本试验旨在研究饲粮中添加活性酵母对生长肉兔生长性能、肉品质和肠道菌群的影响。选取体重相近的健康35日龄断奶仔兔360只,随机分为3组,每组6个重复,每个重复20只仔兔。对照组饲喂基础饲粮(不添加活性酵母),试验组分别在基础饲粮中添加500和1000 g/t的活性酵母。试验期34 d。结果表明:1)与对照组相比,饲粮中添加500和1000 g/t活性酵母显著提高了生长肉兔的成活率(P<0.05),显著降低了生长肉兔的平均日采食量(P<0.05)。2)与对照组相比,饲粮中添加500 g/t活性酵母显著提高了肌肉滴水损失(P<0.05),饲粮中添加1000 g/t活性酵母显著降低了肌肉滴水损失(P<0.05)。3)与对照组相比,饲粮中添加1000 g/t活性酵母显著提高了盲肠微生物的Simpson指数(P<0.05);在门水平上,饲粮中添加1000 g/t活性酵母提高了盲肠厚壁菌门(Firmicutes)的相对丰度;在属水平上,饲粮中添加1000 g/t活性酵母提高了盲肠未分类鞭毛虫菌科(norank_f_Lachnospiraceae)、未分类鼠杆菌科(norank_f_Muribaculaceae)、毛螺旋菌科NK4A136群(Lachnospiraceae_NK4A136_group)、未分类梭状芽孢杆菌属vadinBB60群(norank_f_norank_o_Clostridia_vadinBB60_group)的相对丰度。4)属水平上组间物种差异分析显示,与对照组相比,饲粮中添加500和1000 g/t活性酵母显著降低了盲肠互养菌属(Synergistes)的相对丰度(P<0.05),饲粮中添加500 g/t活性酵母显著提高了盲肠厌氧管菌属(Anaerostipes)的相对丰度(P<0.05),饲粮中添加1000 g/t活性酵母显著提高了盲肠芽孢杆菌属(Bacillus)的相对丰度(P<0.05)。综上所述,饲粮中添加500和1000 g/t活性酵母能够提高生长肉兔的成活率,降低平均日采食量;饲粮中添加1000 g/t活性酵母能降低生长肉兔肌肉滴水损失,从而改善肉质;饲粮中添加1000 g/t活性酵母能改善生长肉兔肠道菌群结构,有助于肠道健康。

关键词：肉兔活性酵母生长性能肉品质肠道菌群

Spatial patterns of phytoplankton communities in an International Seabed Authority licensed area(COMRA,Clarion-Clipperton Zone)in relation to upper ocean biogeochemistry

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Acta Oceanologica Sinica 2022年第11期41卷 45-57页

作者： Yu Wang Aiqin Han Xuebao He Fangfang Kuang Feng zhao Peng xiang Kuidong Xu Third Institute of Oceanography Ministry of Natural ResourcesXiamen 361005China Laboratory of Marine Organism Taxonomy and Phylogeny Chinese Academy of SciencesQingdao 266071China

The Clarion-Clipperton Zone(CCZ)hosts one of the largest known oceanic nodule fields worldwide and is regulated by the International Seabed Authority.A baseline assessment of diversity and distribution patterns is essential for reliable predictions of disturbed ecosystem response scenarios for sustained commercial activities in the *** the present study,the spatial patterns and diversity of phytoplankton communities were analyzed along with upper ocean biogeochemistry,in the licensed China Ocean Mineral Resources R&D Association(COMRA)contract area and the surrounding western CCZ between August 21 and October 8,*** indicated this was a typical low-nutrient low-chlorophyll a(Chl a)environment,characterized by low levels of phytoplankton abundance and *** total 112 species belonging to 4 phyla were recorded(>10μm),with species counts including 82 diatoms,27 dinoflagellates,1 cyanobacteria and 2 *** taxa in successive order of descending abundance and occurrence included Nizschia marina,Cyclotella stylorum,Dactyliosolen mediterraneus,Rhizosolenia setigera,Pseudo-nitzschia delicatissima,Thalassiothrix frauenfeldii,Synedra sp.,Chaetoceros simplex and Pseudo-nitzschia *** depth-averaged abundance and Chl a concentrations were(265±233)cells/L and(0.27±0.30)μg/L,*** accounted for 90.94%of the community with(241±223)cells/L,while dinoflagellates accounted for 5.67%and(15±13)cells/*** distribution pattern exhibited the same trend as abundance,Chl a and species richness,showing subsurface maximum levels at around 100 m,with stations near 10°N having higher levels than in the *** analysis was performed in two assemblages,relating to geographic locations to the south and north of 12°*** subsurface maximum of abundance,Chl a,species richness,dissolved oxygen and nitrite were generally corresponding to the presence of high salinity North Pacific Central Water at depths of 50-120 *** availabilit

关键词： phytoplankton communities spatial patterns diversity upper ocean biogeochemistry Clarion-Clipperton Zone baseline assessment

上海市长宁区居民胃癌和结直肠癌伤残调整寿命年的统计分析

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中华肿瘤杂志 2024年第2期46卷 168-176页

作者：吴景张磊姜玉唐丹丹肖宇轩张云李泓澜赵文穗夏庆华项永兵上海交通大学公共卫生学院上海200025 上海交通大学医学院附属仁济医院上海市肿瘤研究所肿瘤系统医学全国重点实验室上海200032 上海市长宁区疾病预防控制中心上海200051

目的分析上海市长宁区户籍居民胃癌和结直肠癌伤残调整寿命年(DALY)的现况及其变化趋势, 为长宁区胃癌和结直肠癌的防治提供科学依据。方法利用2002—2019年上海市长宁区肿瘤登记数据, 估算该地居民胃癌和结直肠癌的DALYs、粗DALY率、标... 详细信息

目的分析上海市长宁区户籍居民胃癌和结直肠癌伤残调整寿命年(DALY)的现况及其变化趋势, 为长宁区胃癌和结直肠癌的防治提供科学依据。方法利用2002—2019年上海市长宁区肿瘤登记数据, 估算该地居民胃癌和结直肠癌的DALYs、粗DALY率、标化DALY率等指标, 采用Joinpoint回归分析计算标化率的年度变化百分比(APC)和年均变化百分比(AAPC), 描述其不同阶段的时间变化趋势。结果 2002—2019年长宁区胃癌和结直肠癌的DALYs分别为55 931人年和65 252人年, 粗DALY率分别为512.16/10万和597.51/10万;男性疾病负担高于女性, 胃癌粗DALY率峰值分布在75~79岁组, 结直肠癌粗DALY率峰值分布在85岁以上组。Joinpoint回归分析显示, 2002—2019年长宁区胃癌标化DALY率呈下降趋势(AAPC=-3.86%, P0.05), 但结直肠癌标化DALY率在男女性中变化趋势不同, 男性呈上升趋势(AAPC=1.24%, P<0.05), 女性呈下降趋势(AAPC=-1.67%, P<0.05)。结论上海市长宁区居民的胃癌和结直肠癌DALY整体上有所下降, 男性及中老年人群仍是防治重点。

关键词：胃肿瘤结直肠肿瘤疾病负担伤残调整寿命年趋势变化上海市

Enantioselective Synthesis of Tetrahydroquinolines via One-Pot Cascade Biomimetic Reduction

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Chinese Journal of Chemistry 2020年第12期38卷 1691-1695页

作者： Zi-Biao zhao xiang Li Mu-Wang Chen Bo Wu Yong-Gui Zhou State Key Laboratory of Catalysis Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalianLiaoning 116023China State Key Laboratory of Organometallic Chemistry Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai 200032China

of main observation and conclusion A novel and efficient protocol for the synthesis of chiral tetrahydroquinoline derivatives with excellent enantioselectivities and high yields has been developed through one-pot cascade biomimetic *** detailed reaction pathway includes the acid-catalyzed and ruthenium-catalyzed formation of aromatic quinoline intermediates and biomimetic asymmetric reduction.

关键词： reduction. catalyzed quinoline

机插种植均匀度对水稻产量构建及品质形成的影响

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作物学报 2024年第10期50卷 2625-2636页

作者：唐承翰陈惠哲叶天承张玉屏向镜张义凯王志刚王亚梁中国水稻研究所/水稻生物育种全国重点实验室浙江杭州311400

水稻种植均匀度对产量和品质有显著的影响,本试验旨在明确均匀度的提高协同产量和品质提升的机理。试验选用籼粳杂交稻甬优1540,常规粳稻浙禾香2号,杂交籼稻华浙优210为供试品种,设置4种不同种植均匀度处理:T_(1)(条播机插,种植均匀度65... 详细信息

水稻种植均匀度对产量和品质有显著的影响,本试验旨在明确均匀度的提高协同产量和品质提升的机理。试验选用籼粳杂交稻甬优1540,常规粳稻浙禾香2号,杂交籼稻华浙优210为供试品种,设置4种不同种植均匀度处理:T_(1)(条播机插,种植均匀度65%~75%)、T_(2)(撒播机插,种植均匀度45%~55%)、T_(3)(人工模拟机插,种植均匀度100%)、T_(4)(人工模拟机插,种植均匀度50%),比较不同种植均匀度群体的分蘖动态变化、叶面积光合效能、干物质积累与转运、产量形成及稻米品质的变化。结果表明:(1)种植均匀度提高通过促进分蘖增加了群体有效分蘖数,3个品种平均分蘖高峰苗数表现为T_(3)>T_(1)>T_(2)>T_(4),不同品种在处理间变化趋势一致。(2)种植均匀度的提高增加了群体叶面积指数,同时显著提高了抽穗期上三叶高效叶面积指数。而且,增加均匀度显著提高了干物质积累并促进了植株干物质转运,成熟期干物质积累量表现为T_(3)>T_(1)>T_(2)>T_(4),品种间趋势一致,但是T_(2)跟T_(4)差异不明显。均匀度提高对灌浆期茎鞘物质运转有一定促进作用,品种间存在差异,T_(1)、T_(2)和T_(4)间差异不明显。(3)不同均匀度处理下每穗粒数无显著性差异,但高均匀度下一次枝梗和二次枝梗的着粒分布更均匀。(4)高均匀度群体主要通过有效穗数提高产量,品种间趋势一致,T_(3)比T_(1)、T_(2)、T_(4)平均产量分别高8.16%、15.41%、15.61%。(5)提高种植均匀度提高了稻米糙米率、精米率、整精米率和蛋白质含量,降低了稻米垩白米率和垩白度。以上结果表明,提高种植均匀度能够促进群体分蘖,增加群体叶面积指数和干物质积累,提高有效穗数,从而提升了稻谷产量和出米率,并一定程度上改善了稻米品质。试验结果明确生产上采用精准条播育秧机插能够通过提高种植均匀度协同提升稻谷产量和稻米品质。

关键词：水稻种植均匀度播种方式产量稻米品质