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Synthetic anti-angiogenic genomic therapeutics for treatment of neovascular age-related macular degeneration

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Asian Journal of Pharmaceutical Sciences 2021年第5期16卷 623-632页

作者： Jing Wang xiang Shi Qiyu Bo Hong Wang Fang Wei Jun Liu Hao Wang Liuwei Zhang Yan Qi Zhen Li Qixian Chen Xiaodong sun Department of Ophthalmology Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China Shanghai Key Laboratory of Ocular Fundus Diseases Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China Shanghai Engineering Center for Visual Science and Photomedicine Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China National Clinical Research Center for Eye Diseases Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China Ningbo Hygeia Medical Technology Co. Ltd.Ningbo 315201China School of Bioengineering Dalian University of TechnologyDalian 116024China College of Pharmacy Dalian Medical UniversityDalian 116044China

In light of the intriguing potential of anti-angiogenic approach in suppressing choroidal neovascularization, we attempted to elaborate synthetic gene delivery systems encapsulating anti-angiogenic plasmid DNA as alternatives of clinical antibody-based therapeutics. Herein, block copolymer of cyclic Arg-Gly-Asp-poly(ethylene glycol)-poly(lysine-thiol) [RGD-PEG-PLys(thiol)] with multifunctional components was tailored in manufacture of core-shell DNA delivery nanoparticulates. Note that the polycationic PLys segments were electrostatically complexed with anionic plasmid DNA into nanoscaled core, and the tethered biocompatible PEG segments presented as the spatial shell(minimizing non-specific reactions in biological milieu). Furthermore, the aforementioned self-assembly was introduced with redox-responsive disulfide crosslinking due to the thiol coupling. Hence, reversible stabilities, namely stable in extracellular milieu but susceptible to disassemble for liberation of the DNA payloads in intracellular reducing microenvironment, were verified to facilitate transcellular gene transportation. In addition, RGD was installed onto the surface of the proposed self-assemblies with aim of targeted accumulation and internalization into angiogenic endothelial cells given that RGD receptors were specifically overexpressed on their cytomembrane surface. The proposed anti-angiogenic DNA therapeutics were validated to exert efficient expression of anti-angiogenic proteins in endothelial cells and elicit potent inhibition of ocular neovasculature post intravitreous administration. Hence, the present study approved the potential of gene therapy in treatment of choroidal neovascularization. In light of sustainable gene expression properties of DNA therapeutics, our proposed synthetic gene delivery system inspired prosperous potentials in long-term treatment of choroidal neovascularization, which should be emphasized to develop further towards clinical translations.

关键词： Age-related macular degeneration Anti-angiogenesis Gene therapy Polymer Vascular endothelial growth factor

重组C因子法检测医疗器械中细菌内毒素的方法学研究

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食品与药品 2024年第4期26卷 307-311页

作者：秦越孙令骁王国伟孙晓霞员栋良刘增祥焦沁连刘成虎山东省医疗器械和药品包装检验研究院国家药品监督管理局药品包装材料质量控制重点实验室山东济南250101

目的探究重组C因子法检测不同医疗器械中细菌内毒素的适用性,为重组C因子法在医疗器械检验中的使用提供依据。方法以2020年版《中国药典》四部9251细菌内毒素检查法应用指导原则为依据,对重组C因子检测法的准确度进行验证,并按GB/T 16... 详细信息

目的探究重组C因子法检测不同医疗器械中细菌内毒素的适用性,为重组C因子法在医疗器械检验中的使用提供依据。方法以2020年版《中国药典》四部9251细菌内毒素检查法应用指导原则为依据,对重组C因子检测法的准确度进行验证,并按GB/T 16886.1-2011中对医疗器械的分类,选取不同医疗器械样品,使用重组C因子试剂盒进行检测,与凝胶法及动态浊度法进行比对。结果高、中、低浓度内毒素标准品采用重组C因子法的实测值与理论值具有良好的相关性,说明方法具有较好的准确度,且不同医疗器械产品使用重组C因子法和动态浊度法检测结果的比值在50%~200%范围内,回收率在50%~200%之间,与凝胶法检测结果也一致。结论重组C因子法对于医疗器械中细菌内毒素的检测具有良好的适用性。

关键词：细菌内毒素重组C因子法鲎试剂医疗器械

Very long wavelength infrared focal plane arrays with 50% cutoff wavelength based on type-Ⅱ In As/GaSb superlattice

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Chinese Physics B 2017年第1期26卷 563-567页

作者： Xi Han Wei xiang Hong-Yue Hao Dong-Wei Jiang Yao-Yao sun Guo-Wei Wang Ying-Qiang Xu Zhi-Chuan Niu State Key Laboratory for Superlattices and Microstructures Institute of Semiconductors Chinese Academy of Sciences Beijing 100083 China Synergetic Innovation Centre of Quantum Information and Quantum Physics University of Science and Technology of China Hefei 230026 China

A very long wavelength infrared（VLWIR） focal plane array based on In As/Ga Sb type-Ⅱ super-lattices is demonstrated on a Ga Sb substrate. A hetero-structure photodiode was grown with a 50% cut-off wavelength of 15.2 μm, at 77 K.A 320×256 VLWIR focal plane array with this design was fabricated and characterized. The peak quantum efficiency without an antireflective coating was 25.74% at the reverse bias voltage of-20 mV, yielding a peak specific detectivity of 5.89×10^10cm·Hz^1/2·W^-1. The operability and the uniformity of response were 89% and 83.17%. The noise-equivalent temperature difference at 65 K exhibited a minimum at 21.4 mK, corresponding to an average value of 56.3 mK.

关键词： very long wavelength infrared type-Ⅱ InAs/GaSb super-lattices(T2SLs) focal plane array

脂肪酶抑制剂Lipstatin的发酵罐发酵工艺优化

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食品与发酵工业 2014年第9期40卷 63-67页

作者：孙菲福建省微生物研究所福建福州350007

研究了溶氧和剪切力对毒三素链霉菌(Streptomyces tocytricini)发酵产生Lipstatin的影响,并对Lipstatin发酵从摇瓶到10 L发酵罐的放大及补料工艺进行了研究。根据Lipstatin菌株发酵溶氧要求高、对剪切力敏感的特性及高油培养基质地黏稠... 详细信息

研究了溶氧和剪切力对毒三素链霉菌(Streptomyces tocytricini)发酵产生Lipstatin的影响,并对Lipstatin发酵从摇瓶到10 L发酵罐的放大及补料工艺进行了研究。根据Lipstatin菌株发酵溶氧要求高、对剪切力敏感的特性及高油培养基质地黏稠的特点,优化了Lipstatin 10 L全自动发酵罐的搅拌工艺、通气量并建立合适的补料工艺,改善Lipstatin发酵过程中的溶氧水平。Lipstatin 10 L发酵罐发酵效价达到6 446μg/mL,比摇瓶发酵效价提高约40%,发酵周期缩短1 d。

关键词： Lipstatin 10 L发酵罐溶氧补料发酵毒三素链霉菌

Geological emission of methane from the Yakela condensed oil/gas field in Talimu Basin, Xinjiang, China

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Journal of Environmental Sciences 2008年第9期20卷 1055-1062页

作者： Tang Junhong Bao Zhengyu xiang Wu Gou Qinghong China Univ Geosci Minist Educ Key Lab Biogeol & Environm Geol Wuhan 430074 Peoples R China Hangzhou Dianzi Univ Dept Environm Engn & Sci Hangzhou 310018 Peoples R China sun Yat Sen Univ Dept Earth Sci Guangzhou 510275 Guangdong Peoples R China

A static flux chamber method was applied to study natural emissions of methane into the atmosphere in the Yakela condensed oil/gas field in Talimu Basin, Xinjiang, China. Using an online method, which couples a gas chromatography/high-temperature conversion/isotope ratio mass spectrometry (GC/C/MS) together, the 13C/12C ratios of methane in the flux chambers were measured. The results demonstrated that methane gases were liable to migrate from deep oil/gas reservoir to the surface through microseepage and p...

关键词： the Yakela condensed oil/gas field methane emission stable carbon isotopes flux chamber

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马克思劳动价值论的当代价值

当代经济研究 2008年第12期 10-13页

作者：孙飞首都师范大学政法学院北京100089

马克思劳动价值论是马克思主义政治经济学的理论基础,也是马克思主义理论体系的重要组成部分,在经济学说史上具有崇高的历史地位。我国现阶段发展社会主义市场经济,与马克思主义创始人当时所面对和研究的情况有了很大的差别,但马克思劳... 详细信息

马克思劳动价值论是马克思主义政治经济学的理论基础,也是马克思主义理论体系的重要组成部分,在经济学说史上具有崇高的历史地位。我国现阶段发展社会主义市场经济,与马克思主义创始人当时所面对和研究的情况有了很大的差别,但马克思劳动价值论并没有过时。马克思劳动价值论的当代价值在于:它是建立和完善社会主义市场经济的理论基础,是创建现代马克思主义政治经济学的科学基石,是坚定共产主义信念的理论源泉。

关键词：马克思劳动价值论社会主义市场经济现代马克思主义政治经济学共产主义信念

COVID-19 patient with an incubation period of 27 d:A case report

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World Journal of Clinical Cases 2021年第21期9卷 5955-5962页

作者： Xue Du Yang Gao Kai Kang Yang Chong Mei-Ling Zhang Wei Yang Chang-Song Wang xiang-Lin Meng Dong-Sheng fei Qing-Qing Dai Ming-Yan Zhao Department of Critical Care Medicine The First Affiliated Hospital of Harbin Medical UniversityHarbin 150001Heilongjiang ProvinceChina Department of Critical Care Medicine The Sixth Affiliated Hospital of Harbin Medical UniversityHarbin 150028Heilongjiang ProvinceChina Institute of Critical Care Medicine The Sino Russian Medical Research Center of Harbin Medical UniversityHarbin 150081Heilongjiang ProvinceChina Department of Critical Care Medicine The Harbin Medical University Cancer HospitalHarbin 150081Heilongjiang ProvinceChina Department of Critical Care Medicine The Second Affiliated Hospital of Harbin Medical UniversityHarbin 150086Heilongjiang ProvinceChina

BACKGROUND As a highly contagious disease,coronavirus disease 2019(COVID-19)is wreaking havoc around the world due to continuous spread among close contacts mainly via droplets,aerosols,contaminated hands or ***,centralized isolation of close contacts and suspected patients is an important measure to prevent the transmission of *** present,the quarantine duration in most countries is 14 d due to the fact that the incubation period of severe acute respiratory syndrome coronavirus type 2(SARS-CoV-2)is usually identified as 1-14 d with median estimate of 4-7.5 *** COVID-19 patients in the incubation period are also contagious,cases with an incubation period of more than 14 d need to be *** SUMMARY A 70-year-old male patient was admitted to the Department of Respiratory Medicine of The First Affiliated Hospital of Harbin Medical University on April 5 due to a cough with sputum and shortness of *** April 10,the patient was transferred to the Fever Clinic for further treatment due to close contact to one confirmed COVID-19 patient in the same *** the period from April 10 to May 6,nucleic acid and antibodies to SARS-CoV-2 were tested 7 and 4 times,respectively,all of which were *** May 7,the patient developed fever with a maximum temperature of 39℃,and his respiratory difficulties had *** results of nucleic acid and antibody detection of SARS-CoV-2 were *** May 8,the nucleic acid and antibody detection of SARS-CoV-2 by Heilongjiang Provincial Center for Disease Control were also positive,and the patient was diagnosed with COVID-19 and reported to the Chinese Center for Disease Control and *** This case highlights the importance of the SARS-CoV-2 incubation *** epidemiological investigations and clinical observations are urgently needed to identify the optimal incubation period of SARS-CoV-2 and formulate rational and evidence-based quarantine policies for COVID-19

关键词： COVID-19 Incubation period Quarantine duration SARS-CoV-2 Case report

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解析马克思产权理论的三个关键点

经济纵横 2010年第2期 21-25页

作者：孙飞首都师范大学政法学院北京100089

学术界关于是否存在马克思产权理论、马克思产权理论的性质及马克思的所有制理论是否就是他的产权理论等问题一直存在着争论和困惑。本文围绕这三个问题展开讨论,认为存在科学的马克思产权理论,马克思理论中产权的性质具有二重性即包含... 详细信息

学术界关于是否存在马克思产权理论、马克思产权理论的性质及马克思的所有制理论是否就是他的产权理论等问题一直存在着争论和困惑。本文围绕这三个问题展开讨论,认为存在科学的马克思产权理论,马克思理论中产权的性质具有二重性即包含经济关系和法权关系两方面,产权制度是所有制的具体化。

关键词：马克思产权理论存在性二重性所有制的具体化

Gold Nanoparticle Enantiomers and Their Chiral-Morphology Dependence of Cellular Uptake

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CCS Chemistry 2022年第2期4卷 660-670页

作者： Ning-Ning Zhang Hao-Ran sun Shuhan Liu Yu-Chen Xing Jun Lu fei Peng Cheng-Long Han Zhonglin Wei Tianmeng sun Bai Yang Kun Liu State Key Laboratory of Supramolecular Structure and Materials College of ChemistryJilin UniversityChangchun 130012 Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education Institute of ImmunologyThe First HospitalJilin UniversityChangchun 130012 National-Local Joint Engineering Laboratory of Animal Models for Human Diseases Changchun 130012 Department of Chemical Engineering University of MichiganAnn ArborMI 48109 Department of Organic Chemistry College of ChemistryJilin UniversityChangchun 130012 International Center of Future Science Jilin UniversityChangchun 130012 Chiral Nanomaterial Research Center International Center of Future ScienceJilin UniversityChangchun 130012

Chiral molecules are widely prevalent in nature and biological systems,and artificial chiral nanoparticles have drawn enormous interest owing to their unique optical and physical ***,nanoparticles with chiral morphologies and their potential role in biology have been rarely ***,we report a seed-mediated synthesis of enantiomorphic Au nanooctopods(NOPs)and their chiralmorphology dependence of cellular *** a high yield(∼80%),the chiral NOPs possess eight uniform arms that bend fromtodirections,like a propeller *** chiral NOPs synthesized with L-or D-glutathione(GSH)have opposite handedness,resulting in opposite circular dichroism signals,which is consistent with finite-difference time-domain simulations.D-GSH NOPs demonstrate greater than 30%(ca.15%)enhanced cellular uptake in GL261 and bEnd.3 cells compared with L-GSH NOPs(racemic NOPs).Moreover,D-GSH NOPs modified with poly(ethylene glycol)or L-GSH are also preferred by the cells,proving the chiral-morphology dependence of cellular *** study develops the exploration of the chiral-specific interaction in biological systems,providing potential applications for drug delivery,biosensing,and tumor detection.

关键词： chirality branched nanoparticles chiral morphology selective cellular uptake surface plasmonic resonance