检索结果-南通市图书馆

Claudin-4 is required for AMPK-modulated paraceliular permeability in submandibular gland ceils

Journal of Molecular Cell Biology 2014年第6期8卷 486-497页

作者： Ruo-lan xiang Mei Mei Xin Cong Jing Li Yan Zhang Chong Ding Li-Ling Wu Guang-Yan Yu Center for Salivary Gland Diseases of Peking University School and Hospital of Stomatology Department of Physiology and PathophysiologySchool of Basic Medical Sciences Peking University Health Science Center and Key Laboratory of Molecular Cardiovascular Sciences Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology Beijing 100081 China Central Laboratory Peking University School and Hospital of Stomatology Belling 100081 China

Tight junction plays an important rote in mediating paraceUular permeability in epithelia. We previously found that activation of AMP- activated protein kinase （AMPK） increased saliva secretion by modulating paraceUular permeability in submandibular glands. However, the molecular mechanisms underlying AMPK-modulated paraceUular permeability are unknown. In this study, we found that AICAR, an AMPK agonist, increased saliva secretion in the isolated rat submandibular glands, decreased transepithelial electrical resistance （TER）, and increased 4 kDa FITC-dextran flux in cultured SMG-C6 cells. AICAR also induced redistribution of tight junction protein claudin-4, but not claudin-1, claudin-3, occtudin, or ZO-1, from the cytoplasm to the membrane. Moreover, knockdown of claudin-4 by shRNA suppressed while claudin-4 re-expression restored the TER and 4 kDa FITC-dextran flux responses to AICAR. Additionally, AICAR increased ERK1/2 phosphorylation, and inhibition of ERK1/2 by U0126, an ERK1/2 kinase inhibitor, or by siRNA decreased AICAR-induced TER responses. AICAR induced the serine S199 phosphorylation of claudin-4 and enhanced the inter- action of claudin-4 and occludin. Furthermore, pretreatment with U0126 significantly suppressed AMPK-modulated phosphorytation, redistribution, and interaction with occludin of claudin-4. Taken together, these results indicated that claudin-4 played a crucial role in AMPK-rnodutated paraceUular permeability and ERK1/2 was required in AMPK-modulated tight junction barrier function in subman- dibular gland.

关键词： AMP-activated protein kinase daudin-4 tight junction paracellular permeability submandibular gland

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Small-invasive determination of iron content in coating of galvanized steel sheets by laser-induced breakdown spectroscopy

引用

Journal of Iron and Steel Research International 2019年第10期26卷 1137-1146页

作者： lan-xiang Sun Wei-ye Wang Mei-ting Guo Xue-yong Tian Peng Zhang Li-feng Qi Hai-bin Yu Peng Zeng Shenyang Institute of Automation Chinese Academy of SciencesShenyang 110016LiaoningChina Key Laboratory of Networked Control Systems Chinese Academy of SciencesShenyang 110016LiaoningChina Northeastern University Shenyang 110819LiaoningChina Central Research Institute SIASUN Robot&Automation Co.Ltd.Shenyang 110168LiaoningChina University of the Chinese Academy of Sciences Beijing 100049China

A new method was presented to determine the iron content in the coating of galvanized steel sheet based on laser-induced breakdown *** zinc-iron coating was characterized with a series of single laser pulses irradiated on the traversing sheet steel,each on a different steel sheet *** influences of laser fluence and elemental depth distribution were studied and *** protect the corrosion performance of the coating and meet requirements for small-invasive measurement,the ablation size of the crater under different laser fluences was *** the optimized experimental parameters,the diameter of ablation craters is about 50μm,and then,the Fe content in the coating was calibrated and analyzed by the linear standard calibration *** calibration result,however,is not *** that the Zn content in the coating was high and relatively constant,curve calibration was then carried out with the intensity ratio(IFe404.58/Izn468.01)instead of the net line intensity of Fe,and then,the determination coefficient of calibration curve increases from 0.7713 to 0.9511,and the root-mean-square error decreases from 0.4832%to 0.1509%.The results prove that the laser-induced breakdown spectroscopy is an effective way for the analysis of the Fe content in the coating of galvanized steel sheet.

关键词： Laser-induced breakdown spectroscopy Galvanized steel sheet Quantitative analysis Small-invasive analysis Iron content

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Four-year experience with more than 1000 cases of total laparoscopic liver resection in a single center

引用

World Journal of Gastroenterology 2022年第25期28卷 2968-2980页

作者： xiang lan Hai-Li Zhang Hua Zhang Yu-Fu Peng Fei Liu Bo Li Yong-Gang Wei Department of Hepatobiliary Surgery The First Affiliated HospitalChongqing Medical UniversityChongqing 400016China Department of Liver Surgery&Liver Transplantation Center Sichuan University West China HospitalChengdu 610041Sichuan ProvinceChina

BACKGROUND Laparoscopic liver resection(LLR)has become a safe surgical procedure that needs additional *** To review 4 years of total LLR surgeries,exceeding 1000 cases,which were performed at a single *** Patients who underwent LLR at West China Hospital of Sichuan University between January 2015 and December 2018 were *** details,including the interventional year,category of liver disease,and malignant liver tumors prognosis,were *** learning curve for LLR was evaluated using the cumulative sum *** Kaplan-Meier method was used to perform survival *** Ultimately,1098 patients were *** carcinoma(HCC)was the most common disease that led to the need for LLR at the center(n=462,42.08%).The average operation time was 216.94±98.51 *** conversion rate was 1.82%(20/1098).The complication rate was 9.20%(from grade II to V).The 1-year and 3-year overall survival rates of HCC patients were 89.7%and 81.9%,*** learning curve was grouped into two phases for local resection(cases 1-106 and 107-373),three phases for anatomical segmentectomy(cases 1-44,45-74 and 75-120),and three phases for hemihepatectomy(cases 1-17,18-48 and CONCLUSION LLR may be considered a first-line surgical intervention for liver resection that can be performed safely for a variety of primary,secondary,and recurrent liver tumors and for benign diseases once technical competence is proficiently attained.

关键词： Laparoscopic liver resection Single-center experience Learning curve Liver

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Feasibility of using marginal liver grafts in living donor liver transplantation

引用

World Journal of Gastroenterology 2018年第23期24卷 2441-2456页

作者： xiang lan Hua Zhang Hong-Yu Li Ke-Fei Chen Fei Liu Yong-Gang Wei Bo Li Department of Liver Surgery and Liver Transplantation Center West China Hospital of Sichuan University Department of Pancreatic Surgery West China Hospital of Sichuan University

Liver transplantation(LT) is one of the most effective treatments for end-stage liver disease caused by related risk factors when liver resection is contraindicated. Additionally,despite the decrease in the prevalence of hepatitis B virus(HBV) over the past two decades,the absolute number of HBs Ag-positive people has increased,leading to an increase in HBV-related liver cirrhosis and hepatocellular carcinoma. Consequently,a large demand exists for LT. While the wait time for patients on the donor list is,to some degree,shorter due to the development of living donor liver transplantation(LDLT),there is still a shortage of liver grafts. Furthermore,recipients often suffer from emergent conditions,such as liver dysfunction or even hepatic encephalopathy,which can lead to a limited choice in grafts. To expand the pool of available liver grafts,one option is the use of organs that were previously considered "unusable" by many,which are often labeled "marginal" organs. Many previous studies have reported on the possibilities of using marginal grafts in orthotopic LT; however,there is still a lack of discussion on this topic,especially regarding the feasibility of using marginal grafts in LDLT. Therefore,the present review aimed to summarize the feasibility of using marginal liver grafts for LDLT and discuss the possibility of expanding the application of these grafts.

关键词： Marginal liver grafts Living donor liver transplantation Liver transplant waiting lists Small-forsize grafts Older donors ABO-incompatible Steatosis Chronic hepatitis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Potential etiopathogenesis of seventh day syndrome following living donor liver transplantation:ischemia of the graft?

引用

Hepatobiliary & Pancreatic Diseases International 2010年第1期9卷 22-26页

作者： lan, xiang Li, Bo Wang, Xiao-Fei Wei, Yong-Gang Yan, Lu-Nan Zhao, Ji-Chun Sichuan Univ W China Hosp Div Liver Transplantat Chengdu 610041 Peoples R China Sichuan Univ W China Hosp Div Vasc Surg Chengdu 610041 Peoples R China

BACKGROUND: Seventh-day syndrome (7DS) is an early serious complication following liver transplantation with a high mortality because of its unknown etiopathogenesis. This study aimed to analyze the potential etiopathogenesis of 7DS. METHODS: A retrospective analysis of 98 consecutive living donor liver transplants performed from 2001 to 2007 at our center revealed that 5 patients had suffered from 7DS; their age, MELD score, portal vein inflow and other parameters were compared with those of the other recipients. RESULTS: The 5 patients showed common features: (a) initial uneventful recovery, and a dramatic rise of serum liver enzyme levels I to 2 weeks later; (b) decreased inflow in the portal vein accompanied by augmentation of serum creatinine and urea nitrogen level; and (c) serial liver biopsy findings of apoptosis and ischemic necrosis of hepatocytes. Four of the 5 patients died. Age, waiting time to transplantation, MELD score, operation time, cold ischemic time, portal interceptive time and diameter of the portal vein were not significantly different between the 2 groups, but a difference was found in the flow rate of the portal vein (t=3.234, P<0.001). CONCLUSIONS: The 5 patients suffered from a decreased portal vein inflow, ischemic liver necrosis, and renal failure. Hence, hypoperfusion of the liver graft was considered to be the etiopathogenesis of 7DS, for which, however sufficient evidence is lacking. More studies of 7DS are needed.

关键词： seventh-day syndrome living donor liver transplantation ischemic hepatitis etiopathogenesis hepatocellular necrosis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Efficacy of immunosuppression monotherapy after liver transplantation:A meta-analysis

引用

World Journal of Gastroenterology 2014年第34期20卷 12330-12340页

作者： xiang lan Meng-Gang Liu Hong-Xu Chen Hong-Ming Liu Wei Zeng Dong Wei Ping Chen Department of Hepatobiliary Surgery Daping Hospitalthe Third Military Medical University

AIM: To assess the advantages and disadvantages of immunosuppression monotherapy after transplantation and the impact of monotherapy on hepatitis C virus (HCV) recurrence.

关键词： Liver transplantation Immunosuppression monotherapy Cytomegalovirus Diabetes Meta-analysis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Impact of Mental Poverty on Rural Economic Development

引用

Asian Agricultural Research 2011年第10期3卷 35-37,41页

作者： ZHAO lan-xiang Hebei Agricultural University Baoding 071001China

This paper introduces the definition of mental poverty and the status quo of mental poverty in China's rural *** poverty in China's rural areas embodies the following aspects:the sense of parochialism is serious;the small farmer consciousness is strong;there is misgiving about *** paper analyses the reason of mental poverty influencing farmers' behaviour model and rural economic *** poverty influences the farmers' changing current situation;mental poverty influences the rural population structure;mental poverty influences the rural normal *** countermeasures and proposals are put forward to obviate mental poverty in rural areas as follows:first,strengthen farmers' consciousness of main body,and improve farmers' psychological feeling;second,coordinate urban-rural development,and strengthen farmers' social identity degree;third,increase inputs into cultural building,and promote farmers' quality;fourth,open up mind,and encourage the outflow of labour forces.

关键词： Mental poverty Rural economy Material poverty China

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Laser image refractive index sensor based on the combination of a coreless optical fiber and a digital camera

引用

Optoelectronics Letters 2025年第2期21卷 65-68页

作者： GUO Jingzhong lan Feng CHEN xiang LIU Haifeng Xi'an Institute of Space Radio Technology Key Laboratory of Optoelectronic Sensor and Sensing Network Technology College of Electronic Information and Optical Engineering Nankai University

As a laser passes through a scattering medium,the light interacts with the irregular reflections within the medium,resulting in light scattering and the formation of *** this paper,an image sensor based on the combination of a coreless optical fiber and a digital camera is proposed for liquid refractive index sensing *** coreless fiber is used as a sensing unit,and the change in the speckle pattern is measured using the digital correlation method to detect the magnitude of the liquid's refractive *** experimental results indicate that the laser image sensing technique is capable of effectively distinguishing liquid samples with refractive indices ranging from 1.332 8 to1.390 8,with a sensing sensitivity of-1.306 ***,the laser image sensing technique,with its advantages of high experimental reproducibility,simple system design,remote over-control,holds great research significance and potential application in laser communication and sensor integration.

关键词： Digital cameras

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

水热法制备纳米分散颗粒和晶须材料进展

引用

化工学报 2014年第7期65卷 2638-2644页

作者：向兰王靖清华大学化学工程系北京100084

纳米分散颗粒和一维晶须材料性能优越、用途广泛,是近年研究热点。液相法尤其是水热法因具有过程简单和能耗低等特点而被广泛用于制备纳米材料。首先介绍了近年来水热技术在纳米分散颗粒制备中的进展,包括超临界水热法、连续水热法以及... 详细信息

纳米分散颗粒和一维晶须材料性能优越、用途广泛,是近年研究热点。液相法尤其是水热法因具有过程简单和能耗低等特点而被广泛用于制备纳米材料。首先介绍了近年来水热技术在纳米分散颗粒制备中的进展,包括超临界水热法、连续水热法以及水热改性法,并分析了其实现纳米颗粒粒径及分散性控制的原理。然后介绍了两种水热制备晶须材料的新思路,包括水热重结晶法和离子诱导-结构重整法,阐述了其实现晶须定向生长的机制。

关键词：纳米颗粒晶须水热分散定向生长

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

In situ analysis of magnesium alloy using a standoff and double-pulse laser-induced breakdown spectroscopy system

引用

Frontiers of physics 2016年第5期11卷 151-160页

作者： Yong Xin lan-xiang Sun Zhi-Jia Vang Peng Zeng Zhi-Bo Cong Li-Feng Qi Key Laboratory of Networked Control Systems Shenyang Institute of Automation Chinese Academy of Sciences Shenyang 110016 China University of Chinese Academy of Sciences Beijing 100049 China

To monitor the components of molten magnesium alloy during the smelting process in real time and online, we designed a standoff double-pulse laser-induced breakdown spectroscopy （LIBS） analysis system that can perform focusing, collecting and imaging of long-range samples. First, we tested the system on solid standard magnesium alloy samples in the laboratory to establish a basis for the online monitoring of the components of molten magnesium alloy in the future. The experimental results show that the diameters of the focus spots are approximately 1 mm at a range of 3 m, the ablation depth of the double-pulse mode is much deeper than that of the single-pulse mode, the optimum interpulse delay of the double pulse is inconsistent at different ranges, and the spectral intensity decays rapidly as the range increases. In addition, the enhancement effect of the double pulse at 1.89 m is greater than that at 2.97 m, the maximum enhancement is 7.1-fold for the Y（I）550.35-nm line at 1.89 m, and the calibration results at 1.89 m are better than those at 2.97 m. At 1.89 m, the determination coefficients （R2） of the calibration curves are approximately 99% for Y, Pr, and Zr; the relative standard deviations （RSDs） are less than 10% for Y, Pr, and Zr; the root mean square errors （RMSEs） are less than 0.037% for Pr and Zr; the limits of detection （LODs） are less than 1000 ppm for Y, Pr, and Zr; and the LODs of Y, Pr, and Zr at 2.97 m are higher than those at 1.89 m. Additionally, we tested the system on molten magnesium alloy in a magnesium alloy plant. The calibration results of the liquid magnesium alloy are not as favorable as those of the sampling solid magnesium alloys. In particular, the RSDs of the liquid magnesium alloy are approximately 20% for Pr and La. However, with future improvements in the experimental conditions, the developed system is promising for the in situ analysis of molten magnesium alloy.

关键词： laser-induced breakdown spectroscopy standoff double-pulse online magnesium alloy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：