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Induction of apoptosis in human liver carcinoma HepG2 cell line by 5-allyl-7-gen-difluoromethylenechrysin

World Journal of Gastroenterology 2009年第18期15卷 2234-2239页

作者： xiang-Wen Tan hong xia Jin-Hua Xu Jian-Guo Cao Department of Laboratory Animal Science University of South China Hengyang 421001 Hunan ProvinceChina Institute of Cancer Research University of South China Hengyang 421001 Htman Province China Center of Biochemistry and Molecular Biology Laboratory University of South China Hengyang 421001Hunan Province China Laboratory of Medicine Engineering Medical College Hunan Normal University Changsha 410006 Hunan Province China

AIM： To investigate the effect of 5-allyl-7-gen-difluoromethylenechrysin （ADFMChR） on apoptosis of human liver carcinoma HepG2 cell line and the molecular mechanisms ***： HepG2 cells and L-02 cells were cultured in vitro and the inhibitory effect of ADFMChR on their proliferation was measured by MTT assay. The apoptosis of HepG2 cells was determined by flow cytometry （FCM） using propidium iodide （PI） fluorescence staining. DNA ladder bands were observed by DNA agarose gel electrophoresis. The influence of ADFMChR on the proxisome proliferator-activated receptor γ （PPARγ）, NF-κB, Bcl-2 and Bax protein expression of HepG2 cells were analyzed by Western ***： MTT assay showed that ADFMChR significantly inhibited proliferation of HepG2 cells in a dose- dependent manner, with little effect on growth of L-02 cells, and when ICs0 was measured as 8.45 μmol/L and 191.55 μmol/L respectively, the potency of ADFMChR to HepG2 cells, was found to be similar to 5-fluorouracil （5-FU, ICso was 9.27 μmol/L）. The selective index of ADFMChR cytotoxicity to HepG2 cells was 22.67 （191.55/8.45）, higher than 5-FU （SI was 7.05 （65.37/9.27）. FCM with PI staining demonstrated that the apoptosis rates of HepG2 cells treated with 3.0, 10.0 and 30.0 μmol/L ADFMChR for 48 h were 5.79%, 9.29% and 37.8%, respectively, and were significantly higher when treated with 30.0 μmol/L ADFMChR than when treated with 30.0 μmol/L ChR （16.0%）（P 〈 0.05） and were similar to those obtained with 30.0 μmol/L 5-FU（41.0%）. DNA agarose gel electrophoresis showed that treatment of HepG2 cells with 10.0 μmol/L ADFMChR for 48 h and 72 h resulted in typical DNA ladders which could be reversed by 10.00 pmol/1 GW9662, a blocker of PPARy. Western blotting analysis revealed that aEer 24 h of treatment with 3.0, 10.0, 30.0 μmol/L ADFMChR, PPARy and Bax protein expression in HepG2 cells increased but Bcl-2 and NF-κB expression decreased; however, pre-incubation with 10.0 μmol/L GW9662 could efficiently

关键词： Liver neoplasm Chrysin 5-allyl-7-gen-difluoromethylenechrysin Apoptosis Proxisome prolif-erator-activated receptor γ

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Traffic flow prediction based on BILSTM model and data denoising scheme

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Chinese Physics B 2022年第4期31卷 191-200页

作者： Zhong-Yu Li hong-xia Ge Rong-Jun Cheng Faculty of Maritime and Transportation Ningbo UniversityNingbo 315211China Jiangsu Provincial Collaborative Innovation Center for Modern Urban Traffic Technologies Nanjing 210096China National Traffic Management Engineering and Technology Research Center Ningbo University Subcenter Ningbo 315211China

Accurate prediction of road traffic flow is a significant part in the intelligent transportation *** prediction can alleviate traffic congestion,and reduce environmental *** the management department,it can make effective use of road *** individuals,it can help people plan their own travel paths,avoid congestion,and save *** to complex factors on the road,such as damage to the detector and disturbances from environment,the measured traffic volume can contain *** the influence of noise on traffic flow prediction is a piece of very important ***,in this paper we propose a combination algorithm of denoising and BILSTM to effectively improve the performance of traffic flow *** the same time,three denoising algorithms are compared to find the best combination *** this paper,the wavelet(WL) denoising scheme,the empirical mode decomposition(EMD) denoising scheme,and the ensemble empirical mode decomposition(EEMD) denoising scheme are all introduced to suppress outliers in traffic flow *** addition,we combine the denoising schemes with bidirectional long short-term memory(BILSTM)network to predict the traffic *** data in this paper are cited from performance measurement system(PeMS).We choose three kinds of road data(mainline,off ramp,on ramp) to predict traffic *** results for mainline show that data denoising can improve prediction ***,prediction accuracy of BILSTM+EEMD scheme is the highest in the three methods(BILSTM+WL,BILSTM+EMD,BILSTM+EEMD).The results for off ramp and on ramp show the same performance as the results for *** is indicated that this model is suitable for different road sections and long-term prediction.

关键词： traffic flow prediction bidirectional long short-term memory network data denoising

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Recycling of Solanum Sucrose Transporters Expressed in Yeast, Tobacco, and in Mature Phloem Sieve Elements

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Molecular Plant 2010年第6期3卷 1064-1074页

作者： Johannes Liesche hong-xia He Bernhard Grimm Alexander Schulz Christina Kuhn Humboldt University Institute of Biology Plant Physiology Philippstraβe 13 Building 12 10115 Berlin Germany Plant Physiology and Anatomy Laboratory Department of Plant Biology Faculty of Life Sciences Copenhagen University Thorvaldsensvej 40 DK-1871 Frederiksberg C Denmark

The plant sucrose transporter SUT1 （from Solanum tuberosum, S. lycopersicum, or Zea mays） exhibits redoxdependent dimerization and targeting if heterologously expressed in S. cerevisiae （Krtigel et al., 2008）. It was also shown that SUT1 is present in motile vesicles when expressed in tobacco cells and that its targeting to the plasma membrane is reversible. StSUT1 is internalized in the presence of brefeldin A （BFA） in yeast, plant cells, and in mature sieve elements as confirmed by immunolocalization. These results were confirmed here and the dynamics of intracellular SUT1 localization were further elucidated. Inhibitor studies revealed that vesicle movement of SUT1 is actin-dependent. BFA-mediated effects might indicate that anterograde vesicle movement is possible even in mature sieve elements, and could involve components of the cytoskeleton that were previously thought to be absent in SEs. Our results are in contradiction to this old dogma of plant physiology and the potential of mature sieve elements should therefore be re-evaluated. In addition, SUT1 internalization was found to be dependent on the plasma membrane lipid composition. SUT1 belongs to the detergent-resistant membrane （DRM） fraction in planta and is targeted to membrane raft-like microdomains when expressed in yeast （Kr（igel et al,, 2008）, Here, SUT1-GFP expression in different yeast mutants, which were unable to perform en- docytosis and/or raft formation, revealed a strong link between SUT1 raft localization, the sterol composition and mem- brane potential of the yeast plasma membrane, and the capacity of the SUT1 protein to be internalized by endocytosis. The results provide new insight into the regulation of sucrose transport and the mechanism of endocytosis in plant cells.

关键词： Transporters membrane proteins phloem physiology protein targeting potato.

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Thermoelectric properties of (GeTe)_(1-x)[(Ag_(2)Te)_(0.4)(Sb_(2)Te_(3))_(0.6)]_(x) alloys

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Rare Metals 2022年第3期41卷 921-930页

作者： hong-xia Liu Xin-Yue Zhang Zhong-Lin Bu Wen Li Yan-Zhong Pei School of Materials Science and Engineering Taiyuan University of Science and TechnologyTaiyuan 030024China Laboratory of Magnetic and Electric Functional Materials and the Applications The Key Laboratory of Shanxi ProvinceTaiyuan 030024China Interdisciplinary Materials Research Center School of Materials Science and EngineeringTongji UniversityShanghai 201804China

The(GeTe)_(x)(AgSbTe_(2))_(100-x)alloys(TAGS-x)have been demonstrated as a promising p-type candidate for thermoelectric applications,attracting numerous atten-tions on the advancements of thermoelectric *** of carrier concentration for optimizing ther-moelectric performance in TAGS can be achieved by varying the ratio of Ag to Sb,and the Ag/Sb ratio of~2/3 has been proven as the optimal ***,this work focuses on the systematic investigation on ther-moelectric properties of(GeTe)_(1-x)[(Ag_(2)Te)_(0.4)(Sb_(2)Te_(3))_(0.6)]_(x) *** crystal structure for the alloys transfers from rhombohedral to cubic at room temperature as x≥*** evolution of band parameter is estimated using a single parabolic band(SPB)model with acoustic phonon *** density of states effective mass increases with x increasing,which leads to an enhancement of Seebeck coefficient along with a reduction in Hall mobility due to the additional carrier scattering by point ***-while,the lattice thermal conductivity of lower than~0.7 W·m^(-1)·K^(-1) in the entire temperature range and the lowest one of only 0.45 W·m^(-1)·K^(-1) is achieved due to additional phonon scattering by point *** a result,a peak thermoelectric figure of merit(zT)of~1.80 and an average one of~1.37 in 300–800 K are realized in non-stoichiometric TAGS alloys here.

关键词： Thermoelectric Tellurium-antimonygermanium-silver(TAGS) Crystal structure Lattice thermal conductivity

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Experimental Study of Hydrodynamic and Self-Buried Behavior of Submarine Pipeline with Perpendicular Spoilers

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China Ocean Engineering 2021年第2期35卷 250-261页

作者： FAN hong-xia WANG Jian-zhong ZHU Li-jun WANG Nai-ru CHEN Huai State Key Laboratory of Hydrology Water Resources and Hydraulic EngineeringNanjing Hydraulic Research InstituteNanjing 210029China

The spoiler is a kind of device to disturb current and promote *** present,all submarine pipeline spoilers at home and abroad are parallel spoilers,that is,the plane of the spoiler is parallel to the vertical plane of the pipeline *** to the results of indoor experiments,when the pipeline with the forward spoiler is installed perpendicular to the direction of water flow,the spoiler will accelerate the seabed erosion and cause the pipeline to endure downward pressure,which will eventually cause the pipeline self-buried to form a ***,when the pipeline direction is consistent with the flow direction,the self-buried behavior and protective effect is *** aiming at the defect that the forward spoiler cannot be self-buried when the direction of the pipeline and the flow are basically parallel,the spoiler burying aid device perpendicular to the pipeline axis has been innovatively developed,and the hydrodynamic changes and sediment erosion characteristics near the pipeline after the installation of the device were studied based on the *** reveal that although the perpendicular spoiler cannot generate downforce,it can greatly increase the turbulent kinetic energy of the flow and the rate of sediment *** larger the angle between the pipeline axis and the spoiler plane is,the larger the increase in turbulent energy will *** increase in turbulent energy near the bed surface can reach up about 70%when the angle is 90°,while serious sediment erosion mainly occurs along both sides of the pipeline with a distance of about 2−4 times the pipe *** the future,we can further explore the influence of the perpendicular spoiler size and installation position on the pipeline downforce and the effect of burying *** the same time,field tests on the perpendicular spoiler burying aid device currently developed will conduct to observe the actual effect of perpendicular spoiler promoting pipeline scouring and buryi

关键词： submarine pipeline spoiler self-burial turbulent kinetic energy sediment erosion

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Analysis of temporal and spatial distribution characteristics of ammonium chloride smoke particles in confined spaces

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Defence Technology（防务技术） 2022年第7期18卷 1269-1280页

作者： Chen-youshi Xu Bing-ting Zha Jia-qian Bao He Zhang hong-xia Li ZNDY of Ministerial Key Laboratory Nanjing University of Science and TechnologyJiangsuNanjing210094China

In response to the demand for short-range detection of anti-smoke environment interference by laser fuzes,this study proposes a smoke environment simulation of non-uniform continuous point source diffusion and investigates an experimental laboratory smoke environment using an ammonium chloride smoke *** particle size distribution,composition,and mass flow distribution of the smoke were *** on a discrete phase model and a kεturbulence model,a numerical simulation was developed to model the smoke generation and diffusion processes of the smoke agent in a confined *** temporal and spatial distribution characteristics of the smoke mass concentration,velocity,and temperature in the space after smoke generation were analyzed,and the motion law governing the smoke diffusion throughout the entire space was *** with the experimental verification of the smoke environment laboratory,the results showed that the smoke plume changed from fan-shaped to umbrella-shaped during smoke generation,and then continued to spread ***,the mass concentration of smoke in the space decreased from the middle outward;the changes in temperature and velocity were small and *** the diffusion stage(after 900 s),the mass concentration of smoke above 0.8 m was relatively uniform across an area of smoke that was 12 m *** concentration decreased over time,following a consistent decreasing trend,and the attenuation was negligible in a very short ***,this system was suitable for conducting experimental research on laser fuzes in a smoke *** to the stability of the equipment and facilities,the setup could reproduce the same experimental smoke environment by artificially controlling the smoke emission of the smoke ***,this work provides a theoretical reference for subsequent research efforts regarding the construction of uniform smoke environments and evaluating laser transmission characteristics in smoky envir

关键词： Laser fuze Smoke environment Temporal distribution Spatial distribution Confined space Smoke agent

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Maturing process of solitary wave train in a step-down chain

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Chinese Physics B 2012年第2期21卷 368-372页

作者： xia Ji-hong Wang Ping-Jian Liu Chang-Song Key Laboratory of Materials Physics Institute of Solid State PhysicsChinese Academy of SciencesHefei 230031China Department of Physics Chongqing University of Arts and SciencesChongqing 402160China

In a step-down chain a solitary wave （SW） evolves into an SW train （SWT）, but the formation of well-defined SWT takes time and space and little is known of the process from immature into mature SWT. We therefore perform a detailed numerical study of this process by analysing the local velocity amplitude and peak overlap of immature ordered SWs. The first SW continuously increases to maximal velocity amplitude and peak overlap until it is matured, but for following SWs there exist a minimal and maximal value of local velocity amplitude and, a minimal and maximal value of local peak overlap, clarifying the details of the energy propagation along the stepped chain. The immature and mature SWTs show the same dependence of the phase velocity on the SWs sequence. These provide guidelines for when or where the attention should be paid in the study of SWT.

关键词： granular systems solitary wave molecular dynamics simulation

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Genus Ptyobathra Turner new to China,with description of a new species(Lepidoptera:Pyralidae:Phycitinae)

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动物分类学报 2014年第2期39卷 292-296页

作者： hong-xia Liu Hou-Hun Li College of Life Sciences Nankai University

The genus Ptyobathra Turner is newly reported from China. Ptyobathra recta sp. nov. is described as new; P. hypolepidota Turner, 1905 is newly recorded for China. Images of adults and genitalia are provided.

关键词： Lepidoptera Pyralidae Phycitinae Ptyobathra new species new record China.

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Factors affecting the production and regeneration of protoplasts: the case of the mycorrhizal fungus Tulasnella calospora from roots of orchids

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Forestry Studies in China 2013年第3期15卷 186-195页

作者： Li-Kun WANG hong-xia LIU College of Forestry Beijing Forestry University

Protoplast isolation is relevant for many different applications and has been principally used in proceduresnvolving genetic manipulation. In this study, the age of mycelium, osmotic stabilizers, enzyme, incubation temperature and incubation time were evaluated in terms of their effects on protoplast yield. The young mycelia （3 d） of Tulasnella calospora were digested for 6 h at 30℃ in a mixture of 1.2 mol·L-1 MgSO_4 ＋ 10 mmoI·L-1 K2HPO4 as the osmotic stabilizer, with a 1.0% lysing enzyme and 1.5% driselase： more than 106 protoplasts mL-1 were obtained. When collected 3y density gradient centrifugation, the concentration of protoplasts can reach 107-108 protoplasts mL-1, an amount suitable enough for experiments of transformation in fungi. For every 10_5 protoplasts, about 15-25 protoplasts can egenerate after 24-36 h cultivation in a liquid medium and after 8-10 d in an agar medium. This study produced an efficient method for protoplast production, reverting them into a typical mycelia morphology using a Tulasnella calospora solate.

关键词： mycorrhizal fungi Tulasnella calospora protoplasts osmotic stabilizer regeneration

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Taxonomic study of the genus Etiella Zeller(Lepidoptera:Pyralidae:Phycitinae) in China

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动物分类学报 2014年第1期39卷 149-153页

作者： hong-xia Liu Hou-Hun Li College of Life Sciences Nankai University

The genus Etiella Zeller, 1839 is reviewed in China. Etiella brevis sp. nov. is described as new; E. grisea Hampson, 1903 and E. walsinghamella Ragonot, 1888 are newly recorded for China. Images of adults and genitali... 详细信息

关键词： Lepidoptera Pyralidae Phycitinae Etiella new species China.

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