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MiRNA-145-5p inhibits gastric cancer progression via the serpin family E member 1-extracellular signal-regulated kinase-1/2 axis

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World Journal of Gastrointestinal Oncology 2024年第5期16卷 2123-2140页

作者： hong-xia Bai Xue-Mei Qiu Chun-hong Xu Jian-Qiang Guo Department of Gastroenterology The Second Hospital of Shandong UniversityJinan 250000Shandong ProvinceChina Department of Gastroenterology Liaocheng People’s HospitalLiaocheng 252000Shandong ProvinceChina Department of Reproductive Center Zaozhuang Maternal and Child Health Care HospitalZaozhuang 277000Shandong ProvinceChina

BACKGROUND MicroRNAs(miRNAs)regulate gene expression and play a critical role in cancer ***,there is still a limited understanding of the function and regulatory mechanism of miRNAs in gastric cancer(GC).AIM To investigate the role and molecular mechanism of miRNA-145-5p(miR145-5p)in the progression of *** Real-time polymerase chain reaction(RT-PCR)was used to detect miRNA expression in human GC tissues and *** ability of cancer cells to migrate and invade was assessed using wound-healing and transwell assays,*** proliferation was measured using cell counting kit-8 and colony formation assays,and apoptosis was evaluated using flow *** of the epithelial-mesenchymal transition(EMT)-associated protein was determined by Western *** of miR-145-5p were predicated using bioinformatics analysis and verified using a dual-luciferase reporter *** family E member 1(SERPINE1)expression in GC tissues and cells was evaluated using RT-PCR and immunohistochemical *** correlation between SERPINE1 expression and overall patient survival was determined using Kaplan-Meier plot *** association between SERPINE1 and GC progression was also tested.A rescue experiment of SERPINE1 overexpression was conducted to verify the relationship between this protein and *** mechanism by which miR-145-5p influences GC progression was further explored by assessing tumor formation in nude *** GC tissues and cells had reduced miR-145-5p expression and SERPINE1 was identified as a direct target of this *** of miR-145-5p was associated with decreased GC cell proliferation,invasion,migration,and EMT,and these effects were reversed by forcing SERPINE1 ***-Meier plot analysis revealed that patients with higher SERPINE1 expression had a shorter survival rate than those with lower SERPINE1 *** mouse tumorigenesis experiments confirmed that miR-145-5p target

关键词： Gastric cancer MicroRNA-145-5p Serpin family E member 1 Epithelial-mesenchymal transition Proliferation Extracellular signal-regulated kinase-1/2

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Inhibitory Effect of MiR-449b on Cancer Cell Growth and Invasion through LGR4 in Non-Small-Cell Lung Carcinoma

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Current Medical Science 2018年第4期38卷 582-589页

作者： Dong YANG Jin-song LI Qian-yu XU Tian xia Jia-hong xia Department of Thoracic Surger Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China Department of Cariovascular Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China

Non-small-cell lung carcinoma （NSCLC） is one of the most frequently diagnosed malignancies worldwide. Previous studies have shown that microRNA-449b （miR-449b） functions as a tumor suppressor in many cancers. However, the role of miR- 449b in NSCLC is still unknown. In the present study, miR-449b was significantly down- regulated in NSCLC samples and cell lines. Bioinformatics analysis revealed that 3＇-UTR region of leucine rich repeat containing G protein-coupled receptor 4 （LGR4） mRNA had putative complementary sequences to miR-449b, which was further confirmed by the luciferase assay. Western blotting showed that restoration of miR-449b in NSCLC cells decreased the expression of LGR4. Interestingly, over-expression of miR-449b inhibited growth and invasion of NSCLC cells in vitro. Furthermore, ectopic expression of LGR4 reversed miR-449b-suppressed proliferation and invasion of NSCLC cells. Therefore, the data of the present study demonstrate that miR-449b inhibits tumor cell growth and invasion by targeting LGR4 in NSCLC.

关键词： non-small-cell lung carcinoma microRNA-449b leucine rich repeat containing G protein-coupled receptor 4 growth invasion

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Microfabric features of microbial carbonates:experimental and natural evidence of mold holes and crusts

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Journal of Palaeogeography 2021年第3期10卷 321-333页

作者： Ya-Sheng Wu hong-xia Jiang Ying Li Gong-Liang Yu Key Laboratory of Cenozoic Geology and Environment Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China Innovation Academy for Earth Science Chinese Academy of SciencesBeijing 100029China University of Chinese Academy of Sciences Beijing 100049China Institute of Paleontology Hebei GEO UniversityShijiazhuang 050031Hebei ProvinceChina Institute of Hydrobiology Chinese Academy of SciencesWuhan 430072Hubei ProvinceChina

Results of our study based on examination of induced precipitation of carbonate by a cyanobacterium, Lyngbya in the laboratory, and the analyses of microphotographs of both modern and ancient microbial carbonates,demonstrated the importance of recognition of mold holes and carbonate crusts in understanding microbial carbonates. In the experiment, only cyanobacteria Lyngbya can induce precipitation of carbonate, forming scattered grains on the surface of Lyngbya filaments. Carbonate crusts enclosing the old parts of the filaments were formed through aggregation of these scatter grains while mold holes were formed after decay of the filaments. Mainly based on the experiment, six different ways of microbial carbonate formation were recognized:(1) trapping without mold holes,(2) trapping with mold holes,(3) particle-forming induced-precipitation of carbonate,(4) discrete crustforming induced-precipitation of carbonate,(5) induced precipitation, forming tangled crusts that build a porous construction, and(6) induced precipitation, forming a dense construction. And mold holes and crusts can form in ways(4),(5), and(6). Examination of both modern microbial carbonates from the Shark Bay of Australia, Highborne Cay of Bahamas and the atoll of Kiritimati and the microbialites from the Cambrian dolostone sequence in Tarim,Xinjiang, China all demonstrated the limitation of recognizing only mesofabric features and importance of examining microfabric features for understanding of the genesis of the microbial carbonates and their proper classification. The shape, size and arrangement of the mold holes, crusts, and the features of the minerals filling in pores between the crusts, which are referred as the microfabric features here, are keys to better understand the formation and environments of both modern and ancient microbial carbonates.

关键词： Thrombolite Stromatolite Induced calcification Trapping Laminite Proclot

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Thermodynamic simulation of the effect of slag chemistry on the corrosion behavior of alumina–chromia refractory

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International Journal of Minerals,Metallurgy and Materials 2016年第12期23卷 1458-1465页

作者： Shi-xian Zhao Bin-li Cai hong-gang Sun Gang Wang hong-xia Li xiao-yan Song State Key Laboratory of Advanced Refractories Sinosteel Luoyang Institute of Refractories Research Co. Ltd. School of Materials Science and Engineering Beijing University of Technology

The corrosion behavior of alumina-chromia refractory against two kinds of industrial slags （coal slag and iron smelting slag） at 1550℃ was investigated via thermodynamic simulations. In the proposed simulation model, the initial slag first attacks the matrix and surface aggregates and subsequently attacks the inner aggregates. The simulation results indicate that the slag chemistry strongly affects the phase formation and corrosion behavior of the refractory brick. Greater amounts of alumina were dissolved and spinel solid phases formed when the brick interacted with iron smelting slag. These phenomena, as well as the calculated lower viscosity, may lead to much deeper penetration than that exhibited by coal slag and to more severe corrosion compared to that induced by coal slag. The thermodynamic calculations well match the experimental observations, demonstrating the efficiency of the proposed simulation model for evaluating the corrosion behavior of alumina-chromia refractory.

关键词： thermodynamic analysis simulation corrosion spinel alumina chromia refractories

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On Sufficient Conditions for k-Leaf-Connected Graphs

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Journal of Mathematical Research with Applications 2024年第6期44卷 711-722页

作者： Guoyan AO xia hong School of Mathematics and Physics Hulunbuir University Center for Information and Computing Science Hulunbuir University Department of Mathematics Luoyang Normal University

For any integer k ≥ 2, a graph G is called k-leaf-connected if |V(G)| ≥ k + 1 and given any subset S ? V(G) with |S| = k, G always has a spanning tree T such that S is precisely the set of leaves of T. In this paper, we prove best possible sufficient conditions for a graph to be k-leaf-connected in terms of the first Zagreb index, second Zagreb index and hyper-Zagreb index of G or its complement.

关键词： k-leaf-connected Zagreb index Hyper-Zagreb index

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Prox1 Suppresses Proliferation and Drug Resistance of Retinoblastoma Cells via Targeting Notch1

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Current Medical Science 2024年第1期44卷 223-231页

作者： hong-li ZHANG Na LI Lin DONG hong-xia MA Mo-chi YANG Department of Ophthalmology General Hospital of Ningxia Medical UniversityYinchuan 750004China

Objective Retinoblastoma(RB)is a prevalent type of eye cancer in *** homeobox 1(Prox1)is a homeobox transcriptional repressor and downstream target of the proneural gene that is relevant in lymphatic,hepatocyte,pancreatic,heart,lens,retinal,and cancer *** goal of this study was to investigate the role of Prox1 in RB cell proliferation and drug resistance,as well as to explore the underlying Notch1 *** Human RB cell lines(SO-RB50 and Y79)and a primary human retinal microvascular endothelial cell line(ACBRI-181)were used in this *** expression of Prox1 and Notch1 mRNA and protein in RB cells was detected using quantitative real time-polymerase chain reaction(RT-qPCR)and Western *** proliferation was assessed after Prox1 overexpression using the Cell Counting Kit-8 and the MTS ***-resistant cell lines(SO-RB50/vincristine)were generated and treated with Prox1 to investigate the role of Prox1 in drug *** employed pcDNA-Notch1 to overexpress Notch1 to confirm the role of Notch1 in the protective function of ***,a xenograft model was constructed to assess the effect of Prox1 on RB in *** Prox1 was significantly downregulated in RB *** of Prox1 effectively decreased RB cell growth while increasing the sensitivity of drug-resistant cells to ***1 was involved in Prox1’s regulatory ***1 was identified as a target gene of Prox1,which was found to be upregulated in RB cells and repressed by increased Prox1 *** pcDNA-Notch1 was transfected,the effect of Prox1 overexpression on RB was ***,by downregulating Notch1,Prox1 overexpression slowed tumor development and increased vincristine sensitivity in *** These data show that Prox1 decreased RB cell proliferation and drug resistance by targeting Notch1,implying that Prox1 could be a potential therapeutic target for RB.

关键词： Proxl Notch1 retinoblastoma cells proliferation drug resistance

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BL19U2:Small-angle X-ray scattering beamline for biological macromolecules in solution at SSRF

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Nuclear Science and Techniques 2020年第12期31卷 32-40页

作者： Yi-Wen Li Guang-Feng Liu hong-Jin Wu Ping Zhou Chun-xia hong Na Li Feng-Gang Bian Shanghai Advanced Research Institute(Zhangjiang Laboratory) Chinese Academy of SciencesShanghai 201204China CAS Key Laboratory of Molecular Virology and Immunology Institute Pasteur of ShanghaiChinese Academy of SciencesShanghai 200031China University of Chinese Academy of Sciences Beijing 100049China

The BL19U2 at the Shanghai Synchrotron Radiation Facility is a small-angle X-ray scattering beamline dedicated to structural studies pertaining to biological macromolecules in *** beamline has been officially opened to users in March 2015,and since then,a series of technological innovations has been developed to optimize beamline performance,thereby significantly improving the data collection efficiency and broadening the application scope of biological small-angle X-ray ***19U2 is ideal for the high-throughput screening of weakly scattered proteins,protein assemblies,nucleic acids,inorganic nanomaterials,and organic drug *** paper describes the design and overview of the BL19U2 *** sample environments at the experimental station and some recent scientific highlights are presented.

关键词： Shanghai synchrotron radiation facility Biological small-angle X-ray scattering High-throughput screening Biological macromolecules

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A Control Method for Congested Traffic in the Car-Following Model

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Chinese Physics Letters 2012年第5期29卷 49-51页

作者： GE hong-xia YU Jian LU Zhao-Ming Faculty of Science Ningbo UniversityNingbo 315211 Department of Civil and Architectural Engineering City University of Hong KongKowloonHong Kong

A simple control method to suppress traffic congestion is proposed for the car-following *** stability conditions are derived by using the control method,and the feedback signals,which act on our traffic system,are extended to the car-following *** control signals will play an effect only if the traffic is *** corresponding numerical simulation results agree well with our theoretical analysis,and our control method can successfully suppress traffic jams.

关键词： traffic method stability

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Dynamical Process of Dissociation of Excitons in Polymer Chains with Impurities

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Chinese Physics Letters 2011年第9期28卷 221-224页

作者： ZHAO hong-xia ZHAO Hui CHEN Yu-Guang Department of Physics Tongji UniversityShanghai 200092

Within an extended Su–Schrieffer–Heeger model including impurity interactions,the dynamical process of exciton dissociation in the presence of an external electric field is investigated by using a non-adiabatic evolution *** the action of impurities,the stability as well as the effective mass of the exciton is *** results show that the field required to dissociate the excitons depends sensitively on the strength of the impurity *** the impurity potential strength increases,the dissociation field decreases *** theoretical results are expected to provide useful predictions concerning which polymers with properly impurity-assisted interactions are likely to be more suitable for use in organic solar cells.

关键词： potential method dissociation

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AlGaN/GaN Ultraviolet Detector with Dual Band Response

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Chinese Physics Letters 2011年第5期28卷 253-255页

作者： GAO Bo LIU hong-xia WANG Shu-Long School of Microelectronics Key Laboratory of Wide Band-Gap Semiconductor Materials and DevicesXidian UniversityXi’an 710071

The AlGaN/GaN ultraviolet detector with dual band response is investigated by a self-consistent solution of the Poisson-Schr(o)dinger *** of the polarization effect,the AlGaN/GaN UV detector with dual band response can be realized by varying the external *** a low external voltage,the detector is mainly sensitive to the short *** the increasing external voltage is larger than the critical voltage,the detector has an obvious dual band response characteristic and the critical voltage is *** characteristic makes nitride-based UV detector a larger potential application to develop multi band response by adjusting the Aluminum mole fraction in the AlGaN layer or even by using the AlGaN/GaN/InGaN heterostructure.

关键词： AlGaN/GaN polarization AlGaN

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