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I L- 15 trans-presentation regulates homeostasis of CD4^＋ T lymphocytes

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Cellular & Molecular Immunology 2014年第4期11卷 387-395页

作者： xi-lin chen Diwakar Bobbala Yuneivy Cepero Donates Marian Mayhue Subburaj Ilangumaran Sheela Ramanathan Department of Pediatrics Immunology Division Faculty of Medicine and Health Sciences Universite de Sherbrooke Sherbrooke Que Canada Centre de recherche clinique Etienne-Le Bel Sherbrooke QC Canada

Interleukin-15 （IL-15） is essential for the survival of memory CD8^＋ and CD4^＋ T cell subsets, and natural killer and natural killer T cells. Here, we describe a hitherto unreported role of IL-15 in regulating homoeostasis of naive CD4^＋ T cells. Adoptive transfer of splenocytes from non-obese diabetic （NOD） mice results in increased homeostatic expansion of T cells in lymphopenic ***15^-/- mice when compared to *** recipients. The increased accumulation of CD4^＋ T cells is also observed in ***15^-/- mice, indicating that IL-15-dependent regulation also occurs in the absence of lymphopenia. *** mice lacking the I L- 15Ra chain, but not those lacking the common gamma chain, also show increased accumulation of CD4^＋ T cells. These findings indicate that the IL-15-mediated regulation occurs directly on CD4^＋ T cells and requires trans-presentation of IL-15. CD4^＋ T cells expanding in the absence of IL-15 signaling do not acquire the characteristics of classical regulatory T cells. Rather, CD4^＋ T cells expanding in the absence of IL-15 show impaired antigen-induced activation and IFN-7 production. Based on these findings, we propose that the IL-15-dependent regulation of the naive CD4^＋ T-cell compartment may represent an additional layer of control to thwart potentially autoreactive cells that escape central tolerance, while permitting the expansion of memory T cells.

关键词： BDC2.5 CD4^+ T cells homeostasis IL-15 NOD mouse

Gli-1 siRNA induced apoptosis in Huh7 cells

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World Journal of Gastroenterology 2008年第4期14卷 582-589页

作者： xi-lin chen Liang-Qi Cao Miao-Rong She Qian wang xiao-Hui Huang xin-Hui Fu Department of Hepatobiliary Surgery the First Affiliated Hospital of Sun Yat-SenUniversity Guangzhou 510080 Guangdong Province China Department of Hematology GuangdongProvincial People's Hospital Guangzhou 510080 GuangdongProvince China Department of General SurgeryLaboratory the First Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou 510080 Guangdong Province China

AIM： To investigate the effects of Gli-1 small interference RNA （siRNA） on Huh7 cells, and the change of Bcl-2 expression in Huh7 cells. METHODS： Human hepatocellular carcinoma cells Huh7 were used. Cell viability was analyzed by 3-（4, 5-Dimethylthiazol-2-yl）-2, 5-diphenyl tetrazolium bromide （MTT） assay. The expressions of Gli-1 and Bcl-2 family members were detected by RT-PCR and Western blot. Apoptosis was detected by Flow cytometry using propidium iodide, measured by Hoechst 33258 staining using Advanced Fluorescence Microscopy and caspase-3 enzymatic assay. Cell growth was analyzed after treatment with Gli-1 siRNA and 5-fluorouracil （5-Fu）. RESULTS： Inhibition of Gli-1 mRNA in Huh7 cells through Gli-1 siRNA reduced cell viability. Gli-1 siRNA treatment also induced apoptosis by three criteria, increase in the sub-G1 cell cycle fraction, nuclear condensation, a morphologic change typical of apoptosis, and activation of caspase-3. Gli-1 siRNA was also able to down-regulate Bcl-2. However, Gli-1 siRNA resulted in no significant changes in Bcl-xl, Bax, Bad, and Bid. Furthermore, Gli-1 siRNA increased the cytotoxic effect of 5-Fu on Huh7 cell. CONCLUSION： Down-regulation of Bcl-2 plays an important role in apoptosis induced by Gli-1 siRNA in HCC cells. Combination Gli-1 siRNA with chemotherapeutic drug could represent a more promising strategy against HCC. The effects of the strategies need further investigation in vivo and may have potential clinical application.

关键词： Gli-1 transcription factor Small interferenceRNA Apoptosis Hepatocellular carcinoma 5-fluorouracil

Towards human-like and transhuman perception in AI 2.0:a review

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Frontiers of Information Technology & Electronic Engineering 2017年第1期18卷 58-67页

作者： Yong-hong TIAN xi-lin chen Hong-kai xiONG Hong-liang LI Li-rong DAI Jing chen Jun-liang xiNG xi-hong WU Wei-min HU Yu HU Tie-jun HUANG Wen GAO School of Electronics Engineering and Computer Science Peking University Institute of Computing Technology Chinese Academy of Sciences Department of Electronic Engineering Shanghai Jiao Tong University School of Electronic Engineering University of Electronic Science and Technology of China Department of Electronic Engineering and Information Sciences University of Science and Technology of China School of Optoelectronics Beijing Institute of Technology Institute of Automation Chinese Academy of Sciences

Perception is the interaction interface between an intelligent system and the real world. Without sophisticated and flexible perceptual capabilities, it is impossible to create advanced artificial intelligence （AI） systems. For the next-generation AI, called ＇AI 2.0＇, one of the most significant features will be that AI is empowered with intelligent perceptual capabilities, which can simulate human brain＇s mechanisms and are likely to surpass human brain in terms of performance. In this paper, we briefly review the state-of-the-art advances across different areas of perception, including visual perception, auditory perception, speech perception, and perceptual information processing and learning engines. On this basis, we envision several R＆D trends in intelligent perception for the forthcoming era of AI 2.0, including：（1） human-like and transhuman active vision; （2） auditory perception and computation in an actual auditory setting; （3） speech perception and computation in a natural interaction setting; （4） autonomous learning of perceptual information; （5） large-scale perceptual information processing and learning platforms; and （6） urban omnidirectional intelligent perception and reasoning engines. We believe these research directions should be highlighted in the future plans for AI 2.0.

关键词： Intelligent perception Active vision Auditory perception Speech perception Autonomous learning

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混合动力推进轴系扭转振动研究

船舶力学 2016年第10期20卷 1309-1319页

作者：周瑞平肖能齐林晞晨武汉理工大学能源与动力工程学院武汉430063

文章对混合动力推进轴系特点进行分析,采用集总参数法建立轴系扭转振动数学模型及其振动方程。着重讨论了混合动力推进轴系并车相位角和电机激励对轴系扭转振动的影响以及自主开发的振动测试系统。以混合动力推进轴系试验台架为基础,将... 详细信息

文章对混合动力推进轴系特点进行分析,采用集总参数法建立轴系扭转振动数学模型及其振动方程。着重讨论了混合动力推进轴系并车相位角和电机激励对轴系扭转振动的影响以及自主开发的振动测试系统。以混合动力推进轴系试验台架为基础,将台架测试值与理论计算值进行对比分析,验证了混合动力推进轴系数学模型和方法的正确性。

关键词：混合动力电机推进轴系相位角

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连接件不对中对轴系回旋振动的影响特性研究

船舶力学 2016年第7期20卷 866-873页

作者：林晞晨周瑞平肖能齐武汉理工大学能源与动力工程学院武汉430063

船舶推进轴系是船舶动力装置的重要组成部分,在航行过程中会受到多种复杂载荷的作用。文章基于轴心与质心的相对位置关系,推导了连接件不对中所导致的不平衡力,以及轴系回旋振动的受迫响应振幅数学模型。分别以转子轴系试验台及某船轴... 详细信息

船舶推进轴系是船舶动力装置的重要组成部分,在航行过程中会受到多种复杂载荷的作用。文章基于轴心与质心的相对位置关系,推导了连接件不对中所导致的不平衡力,以及轴系回旋振动的受迫响应振幅数学模型。分别以转子轴系试验台及某船轴系为对象,进行了仿真计算与试验测试。计算结果表明:连接件不对中使轴系振动响应振幅提高,具有较为明显的影响。

关键词：推进轴系回旋振动连接件不对中响应振幅

Tranylcypromine upregulates Sestrin 2 expression to ameliorate NLRP3-related noise-induced hearing loss

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Neural Regeneration Research 2025年第5期20卷 1483-1494页

作者： xihang chen Zhifeng chen Menghua Li Weiwei Guo Shuolong Yuan Liangwei Xu Chang lin xi Shi Wei chen Shiming Yang Senior Department of Otolaryngology Head and Neck Surgery Chinese PLA General HospitalChinese PLA Medical SchoolBeijingChina State Key Laboratory of Hearing and Balance Science BeijingChina National Clinical Research Center for Otolaryngologic Diseases BeijingChina Key Laboratory of Hearing Science Ministry of EducationBeijingChina Beijing Key Laboratory of Hearing Impairment Prevention and Treatment BeijingChina Department of Otorhinolaryngology Head and Neck Surgery The First Affiliated Hospital of Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Otolaryngology Head and Neck Surgery The 940th Hospital of Joint Logistics Support Force of Chinese PLALanzhouGansu ProvinceChina Key Laboratory of Tropical Biological Resources of Ministry of Education School of Pharmaceutical SciencesHainan UniversityHaikouHainan ProvinceChina Academician Workstation of Hainan University School of Pharmaceutical SciencesYazhou BaySanyaHainan ProvinceChina

Noise-induced hearing loss is the primary non-genetic factor contributing to auditory ***,there are currently no effective pharmacological interventions for patients with noise-induced hearing ***,we present evidence suggesting that the lysine-specific demethylase 1 inhibitor–tranylcypromine is an otoprotective agent that could be used to treat noise-induced hearing loss,and elucidate its underlying regulatory *** established a mouse model of permanent threshold shift hearing loss by exposing the mice to white broadband noise at a sound pressure level of 120 d B for 4 *** found that tranylcypromine treatment led to the upregulation of Sestrin2(SESN2)and activation of the autophagy markers light chain 3B and lysosome-associated membrane glycoprotein 1 in the cochleae of mice treated with *** noise exposure group treated with tranylcypromine showed significantly lower average auditory brainstem response hearing thresholds at click,4,8,and 16 k Hz frequencies compared with the noise exposure group treated with *** findings indicate that tranylcypromine treatment resulted in increased SESN2,light chain 3B,and lysosome-associated membrane glycoprotein 1 expression after noise exposure,leading to a reduction in levels of 4-hydroxynonenal and cleaved caspase-3,thereby reducing noise-induced hair cell ***,immunoblot analysis demonstrated that treatment with tranylcypromine upregulated SESN2 expression via the autophagy *** treatment also reduced the production of NOD-like receptor family pyrin domaincontaining 3(NLRP3)*** conclusion,our results showed that tranylcypromine treatment ameliorated cochlear inflammation by promoting the expression of SESN2,which induced autophagy,thereby restricting NLRP3-related inflammasome signaling,alleviating cochlear hair cell loss,and protecting hearing *** findings suggest that inhibiting lysine-specific demethylase 1 is a

关键词： 4-hydroxynonenal apoptosis autophagy cleaved caspase-3 inflammation NOD-like receptor family pyrin domain-containing 3(NLRP3) noise-induced hearing loss oxidative stress Sestrin2 tranylcypromine

Upregulation of PTEN involved in rosiglitazone-induced apoptosis in human hepatocellular carcinoma cells

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Acta Pharmacologica Sinica 2007年第6期28卷 879-887页

作者： Liang-qi CAO~2,xi-lin chen~2,Qian WANG~3,xiao-hui HUANG,Mao-chuan ZHEN,Long-juan ZHANG,Wen LI,Jiong BIDepartment of Hepatobiliary Surgery the First Affiliated Hospital of Sun Yat-Sen University,Guangzhou 510080,China Department of Hepatobiliary Surgery the First Affiliated Hospital of Sun Yat-Sen University Guangzhou China

Aim:To investigate the effects of rosiglitazone,a peroxisome proliferator-ac-tivated receptor gamma (PPART) agonist,on the expression of the phosphataseand tensin homologue deleted on chromosome 10 gene (PTEN) and cell growthin hepatocellular carcinoma cells,as well as the underlying mechanisms of ***:RT-PCR and Western blotting analyses were performed to de-tect transcription and the expression of PTEN in Hep3B cells treated withrosiglitazone.3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide as-say was used to evaluate cell *** cytometry,DNA fragmentation analysis,caspase enzymatic assay,and Hoechst 33258 staining were used to determine ***,small interfering RNA was used to suppress ***:Rosiglitazone increased the expression of PTEN in a dose-and time-dependent manner through the PPARγ-dependent signal *** upregulation was concomitant with a decreased level of Aktphosphorylation,subsequently resulting in cell growth inhibition and apoptosisin Hep3B *** knockdown dramatically blocked these effects ***,the exposure of cells to rosiglitazone activated caspases-9and-3 during apoptotic ***:Thus,upregulation of PTENis involved in the inhibition of cell growth and the induction of cell apoptosis byrosiglitazone,suggesting that rosiglitazone may be useful in liver cancer therapyvia apoptosis.

关键词： rosiglitazone PTEN apoptosis hepato cellular carcinoma PPARγ

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基于CFX的螺旋桨激振力数值预报研究

武汉理工大学学报 2014年第7期36卷 73-79页

作者：陈如星周瑞平林晞晨武汉理工大学能源与动力工程学院武汉430063

基于CFX的滑移网格技术预报伴流场下螺旋桨的非定常水动力性能,为螺旋桨激振力数值计算提供可行的思路。以桨舵系统为计算分析对象,比较分析了均匀来流下螺旋桨的定常和非定常水动力性能的差别;计算了理论伴流下螺旋桨的非定常水动力性... 详细信息

基于CFX的滑移网格技术预报伴流场下螺旋桨的非定常水动力性能,为螺旋桨激振力数值计算提供可行的思路。以桨舵系统为计算分析对象,比较分析了均匀来流下螺旋桨的定常和非定常水动力性能的差别;计算了理论伴流下螺旋桨的非定常水动力性能,将所得结果与理论伴流的趋势进行了比较分析,验证了CFD计算模型的准确性。预报伴流场下螺旋桨的激振力,将计算结果与文献所给建议值进行了比较分析。结果表明,该方法可有效预报螺旋桨激振力,能够代替经验公式求解螺旋桨激振扭矩。

关键词：非定常水动力性能螺旋桨激振力桨舵系统伴流 CFD

Interannual variability of boreal summer intraseasonal oscillation over the northwestern Pacific influenced by the Pacific Meridional Mode

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Atmospheric and Oceanic Science Letters 2025年第1期18卷 6-11页

作者： Haoyu Zhou Pang-Chi Hsu lin chen Yitian Qian Key Laboratory of Meteorological Disaster Ministry of Education/Joint International Research Laboratory of Climate and Environmental Change/Collaborative Innovation Center on Forecast and Evaluation of Meteorological DisastersNanjing University of Information Science and TechnologyNanjingChina

During the boreal summer,intraseasonal oscillations exhibit significant interannual variations in intensity over two key regions:the central-western equatorial Pacific(5°S-5°N,150°E-150°W)and the subtropical Northwestern Pacific(10°-20°N,130°E-175°W).The former is well-documented and considered to be influenced by the ENSO,while the latter has received comparatively less attention and is likely influenced by the Pacific Meridional Mode(PMM),as suggested by partial correlation analysis *** elucidate the physical processes responsible for the enhanced(weakened)intraseasonal convection over the subtropical northwestern Pacific during warm(cold)PMM years,the authors employed a moisture budget *** findings reveal that during warm PMM years,there is an increase in summer-mean moisture over the subtropical northwestern *** increase interacts with intensified vertical motion perturbations in the region,leading to greater vertical moisture advection in the lower troposphere and consequently resulting in convective *** a process is pivotal in amplifying intraseasonal convection *** observational findings were further verified by model experiments forced by PMM-like sea surface temperature patterns.

关键词： Boreal summer intraseasonal oscillation Interannual variability Pacific Meridional Mode Moisture budget analysis