检索结果-南通市图书馆

Soluble expression of active human β-defensin-3 in Escherichia coli and its effects on the growth of host cells

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2007年第8期120卷 708-713页

作者： SI Li-gang LIU xi-cheng LU You-yong WANG Gen-yu LI Wen-mei Beijing Pediatric Research Institute Beijing Children＇s Hospital Affiliated to Capital Medical University Beijing 100045 China Molecular Oncology Laboratory Beijing Institute for Cancer Research School of Oncology Peking University Beijing 100034 China

Background Human β-defensin-3 （HBD3） is an epithelial peptide that has been demonstrated to have a salt-insensitive broad spectrum of potent antimicrobial activity. Expressing antimicrobial peptides in Escherichia coil （E. colt） is very difficult for it can result in death of the bacterial host cells. Our aim was to establish a prokaryotic system expressing soluble HBD3 protein and demonstrate the antimicrobial activity of the expressed protein. We then studied whether the host cells would activate the suicide pathways. Methods We first cloned the complementary DNA coding for the mature chain of HBD3, inserted it into the vector PGEX-KG then transformed E. coil BL21 （DE3） with the appropriate recombinant plasmid. After induction with 0.5 mmol/L isopropyl-1-thio-β-D-galactopyranoside （IPTG） the transformed E. cofiproduced a recombinant glutathione S-transferase and HBD3 （GST-HBD3） fusion protein. The fusion protein was treated with thrombin to produce pure HBD3 protein then the antimicrobial activity of HBD3 was evaluated in a liquid microdilution assay. Results The fusion protein GST-HBD3 was efficiently cleaved by thrombin and yielded HBD3 that had anti-staphylococcus aureus activity with a minimal inhibitory concentration level of 12.5 μg/ml. The E. coil strain expressing the recombinant protein did not grow slower than the empty vector strain. Conclusion Active HBD3 in E. coil by expressing the recombinant protein GST-HBD3 could be produced, and suicide did not occur in the E. coli strain expressing the recombinant protein.

关键词： human beta-defensin-3 antimicrobial peptide prokaryotic expression

Layer-Wise Pre-Training Low-Rank NMF Model for Mammogram-Based Breast Tumor Classification

在线全文

学校读者我要写书评

暂无评论

Journal of the Operations Research Society of China 2019年第4期7卷 515-537页

作者： Wen-Ming Wu xiao-Hui Yang Yun-Mei Chen Juan zhang Dan Long Li-Jun Yang Chen-xi Tian Data Analysis Technology Lab Institute of Applied MathematicsSchool of Mathematics and StatisticsHenan UniversityKaifeng 475004HenanChina Department of Mathematics University of FloridaGainesvilleFL 32611USA Zhejiang Cancer Hospital Hangzhou 310022China

Image-based breast tumor classification is an active and challenging *** this paper,a robust breast tumor classification framework is presented based on deep feature representation learning and exploiting available information in existing *** representation learning of mammograms is fulfilled by a modified nonnegative matrix factorization model called LPML-LRNMF,which is motivated by hierarchical learning and layer-wise pre-training(LP)strategy in deep ***-rank(LR)constraint is integrated into the feature representation learning model by considering the intrinsic characteristics of ***,the proposed LPML-LRNMF model is optimized via alternating direction method of multipliers and the corresponding convergence is *** completing classification,an inverse projection sparse representation model is introduced to exploit information embedded in existing samples,especially in test *** on the public dataset and actual clinical dataset show that the classification accuracy,specificity and sensitivity achieve the clinical acceptance level.

关键词： Breast tumor classification Mammogram LPML-LRNMF Inverse space sparse representation ADMM

A Novel CA4P Polymeric Nanoparticle for Murine Hepatoma Therapy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Polymer Science 2023年第8期41卷 1223-1229,I0007页

作者： Zhi-Lin Liu xi-Tong Ren Yue Huang Jia-Li Sun xiao-Shuang Wang Meng-Fei Zheng Lin-Jie Cui Xue-Fei zhang Zhao-Hui Tang Key Laboratory of Polymer Ecomaterials Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China Jilin Biomedical Polymers Engineering Laboratory Changchun 130022China School of Applied Chemistry and Engineering University of Science and Technology of ChinaHefei 230026China No.1 Department of Neurology China-Japan Union Hospital of Jilin UniversityChangchun 130033China Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education and Key Laboratory of Polymeric Materials&Application Technology of Hunan Province College of ChemistryXiangtan UniversityXiangtan 411105China

Combretastatin A4 phosphate(CA4P)is a potent vascular disrupting agent with good water ***,it is only effective at high doses,which decreases clinical ***,we designed stable CA4P polymeric nanoparticles(CA4P NPs)consisting of various cholesterol derivatives,and with a drug loading efficacy of 93%.The nanoparticles released CA4P in a sustained manner and achieved a 72%inhibition rate in the murine H22 liver tumor model,which was about 2.9-fold higher than that of free CA4P(24.6%).Furthermore,the carrier components of CA4P NPs were metabolized to arginine,cholesterol,ethanol and poly(ethylene glycol)in vivo;therefore,the CA4P NPs are safe and have significant potential for clinical translation.

关键词： CA4P polymeric nanoparticle Cholesterol derivatives Vascular disrupting agent Phosphate-guanidine coordination Drug controlled release

Economic Analysis on Monetization of Soil Functions

在线全文

学校读者我要写书评

暂无评论

Asian Agricultural Research 2013年第5期5卷 6-8页

作者： Zenglei xi Shaoqing zhang School of Earth Science and Technology China University of Geosciences Department of Economics and Management Xingtai Polytechnic College

On the basis of making clear diversity characteristics of soil functions and multiple characteristics of income, this paper points out that the monetization of soil functions based functional maintenance and change decision process can be regarded as a game process of different utilization methods at the background of different functions. The balance of this game process will determine monetary value of soil functions. After understanding money and monetization concepts, it introduces that measurability and exchangeability of soil functions provide objective conditions for monetization of soil functions. Finally, it discusses that usefulness value of soil functions provide basis for monetization of soil functions.

关键词： Soil functions Monetization Economic analysis

Exploration of the mechanism by which icariin modulates hippocampal neurogenesis in a rat model of depression

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2022年第3期17卷 632-642页

作者： Ning-xi Zeng Hui-Zhen Li Han-zhang Wang Kai-Ge Liu xia-Yu Gong Wu-Long Luo Can Yan Li-Li Wu Research Center for Basic Integrative Medicine Guangzhou University of Chinese MedicineGuangzhouGuangdong ProvinceChina

Icariin(ICA) has a significant capacity to protect against depression and hippocampal injury,but it cannot effectively cross the bloodbrain barrier and accumulate in the ***,the mechanism by which ICA protects against hippocampal injury in depression remains *** this study,we performed proteomics analysis of cerebrospinal fluid to investigate the mechanism by which ICA prevents dysfunctional hippocampal neurogenesis in depression.A rat model of depression was established through exposure to chronic unpredictable mild stress for 6 weeks,after which 120 mg/kg ICA was administered subcutaneously every *** results showed that ICA alleviated depressive symptoms,learning and memory dysfunction,dysfunctional neurogenesis,and neuronal loss in the dentate gyrus of rats with *** stem cells from rat embryonic hippocampi were cultured in media containing 20% cerebrospinal fluid from each group of rats and then treated with 100 μM *** addition of cerebrospinal fluid from rats treated with ICA largely prevented the corticosterone-mediated inhibition of neuronal proliferation and ***-two differentially expressed proteins regulated by chronic unpredictable mild stress and ICA were identified through proteomics analysis of cerebrospinal *** proteins were mainly involved in the ribosome,PI3 K-Akt signaling,and interleukin-17 signaling *** reaction monitoring mass spectrometry showed that Rps4 x,Rps12,Rps14,Rps19,Hsp90 b1,and Hsp90 aa1 were up-regulated by chronic unpredictable mild stress and down-regulated by *** contrast,Htr A1 was down-regulated by chronic unpredictable mild stress and up-regulated by *** findings suggest that ICA can prevent depression and dysfunctional hippocampal neurogenesis through regulating the expression of certain proteins found in the cerebrospinal *** study was approved by the Experimental Animal Ethics Committee of Guangzhou University of Chinese

关键词： cerebrospinal fluid chronic unpredictable mild stress depression dysfunctional hippocampal neurogenesis hippocampus icariin proteomics ribosome pathway

CD 147 receptor is essential for TFF3-mediated signaling regulating colorectal cancer progression

在线全文

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2021年第8期6卷 2521-2535页

作者： Hong-Yong Cui Shi-Jie Wang Fei Song Xu Cheng gang Nan Yu Zhao Mei-Rui Qian xi Chen Jia-Yue Li Fen-Ling Liu Yu-Meng Zhu Ruo-Fei Tian Bin Wang Bin Wu Yang zhang xiu-Xuan Sun Ting Guo xiang-Min Yang Hai zhang Ling Li Jing Xu Hui-Jie Bian Jian-Li Jiang Zhi-Nan Chen National Translational Science Center for Molecular Medicine and Department of Cell Biology Fourth Military Medical UniversityXi'anChina Department of Urology Shenzhen Institute of Translational MedicineThe First Affiliated Hospital of Shenzhen UniversityShenzhen Second People's HospitalShenzhenChina Medical Research Institute School of MedicineWuhan UniversityWuhanChina College of Chemistry and Materials Science Northwest UniversityXi'anChina National Facility for Protein Science Shanghai Zhangjiang LabShanghai Advanced Research InstituteChinese Academy of SciencesShanghaiChina Medical Research Center Southern University of Science and Technology HospitalShenzhenChina

Major gaps in understanding the molecular mechanisms of colorectal cancer(CRC)progression and intestinal mucosal repair have hampered therapeutic development for gastrointestinal *** factor 3(TFF3)has been reported to be involved in CRC progression and intestinal mucosal repair;however,how TFF3 drives tumors to become more aggressive or metastatic and how TFF3 promotes intestinal mucosal repair are still poorly ***,we found that the upregulated TFF3 in CRC predicted a worse overall survival ***3 deficiency impaired mucosal restitution and ***147,a membrane protein,was identified as a binding partner for *** binding to CD147,TFF3 enhanced CD147-CD44s interaction,resulting in signal transducer and activator of transcription 3(STAT3)activation and prostaglandin G/H synthase 2(PTGS2)expression,which were indispensable for TFF3-induced migration,proliferation,and ***2-derived PGE2 bound to prostaglandin E2 receptor EP4 subtype(PTGER4)and contributed to TFF3-stimulated CRC *** NMR studies of the TFF3-CD147 interaction revealed the key residues critical for TFF3 binding and the induction of PTGS2 *** ability of TFF3 to enhance mucosal restitution was weakened by a PTGS2 *** of TFF3-CD147 signaling using competitive inhibitory antibodies or a PTGS2 inhibitor reduced CRC lung metastasis in *** findings bring strong evidence that CD147 is a novel receptor for TFF3 and PTGS2 signaling is critical for TFF3-induced mucosal restitution and CRC progression,which widens and deepens the understanding of the molecular function of trefoil factors.

关键词： TFF3 colorectal impaired

Unraveling the relationship between Sr stoichiometry in Sr_(x)Fe_(1.5)Mo_(0.5)O_(6)-σ and its catalytic performance for high-temperature CO_(2) electrolysis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Materials Reports(Energy) 2023年第1期3卷 86-92,I0003页

作者： xiuan xi xiaoyu Liu Lingui Huang Jianwen Liu Bo-Wen zhang Gadi Rothenberg xian-Zhu Fu Jing-Li Luo College of Materials Science and Engineering Shenzhen UniversityShenzhen518060China Department of Chemical and Materials Engineering University of AlbertaEdmontonABT6G 1H9Canada Van‘t Hoff Institute for Molecular Sciences University of AmsterdamScience Park 9041098XHAmsterdamthe Netherlands

The solid oxide electrolytic cell(SOEC)is one of the most promising energy conversion and storage devices,which could convert CO_(2) to CO with high Faradaic efficiency and production ***,the lack of active and stable cathode materials impedes their practical *** we focus on the promising perovskite oxide cathode material Sr_(2)Fe_(1.5)Mo_(0.5)O_(6)-σ,with the aim of understanding how A-atom stoichiometry and catalytic performance are *** find that increasing the strontium content in the perovskite improves the chemisorption of CO_(2) on its surface,forming a SrCO_(3) *** hinders the charge transfer and oxygen exchange ***-neously,strontoium segregation to the cathode surface facilitates coking of the surface during CO_(2) electrolysis,which poisons the ***,a small number of Sr deficiencies are optimal for both electrochemical performance and long-term *** results provide new insights for designing high-performance CO_(2) electrolysis cathode materials.

关键词： SOECs Perovskite oxides Cathode Sr segregation CO_(2) electrolysis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

地面多目标快速响应任务轨道设计

宇航学报 2023年第5期44卷 677-686页

作者：张海洋张刚哈尔滨工业大学航天学院卫星技术研究所哈尔滨150001

针对多目标快速响应对地成像任务,提出了一种J_(2)摄动下4个地面目标重复访问以及5个地面目标单次访问的轨道设计方法。首先在轨道倾角已知的假设下,通过第1个目标点的访问约束解析得到升交点赤经和参数纬度幅角,然后通过第2、3、4个目... 详细信息

针对多目标快速响应对地成像任务,提出了一种J_(2)摄动下4个地面目标重复访问以及5个地面目标单次访问的轨道设计方法。首先在轨道倾角已知的假设下,通过第1个目标点的访问约束解析得到升交点赤经和参数纬度幅角,然后通过第2、3、4个目标点的访问约束数值求解得到近地点角距、偏心率和半长轴。最后,通过增加回归轨道约束或第5个目标点的访问约束,将轨道设计问题转化为只含有倾角的一维非线性方程,通过一维数值搜索进行求解。仿真结果表明,通过所提方法设计轨道的星下点轨迹能够精确经过指定的多个地面目标,验证了方法的有效性和正确性。

关键词：快速响应空间多目标点星下点轨迹轨道设计 J2摄动

数字闭环石英挠性加速度计信号检测技术(英文)

在线全文

学校读者我要写书评

暂无评论

红外与激光工程 2014年第10期43卷 3356-3362页

作者：张晞张声艳北京航空航天大学仪器科学与光电工程学院北京100191 中国运载火箭技术研究院研究发展中心北京100076

数字闭环石英挠性加速度计可以有效地解决传统模拟式加速度计在模数转换中引起的精度损失问题,而数字式加速度计的精度主要取决于差动电容检测环节。阐述了数字式加速度计的工作原理,重点设计了差动电容检测方法,利用单载波调制与数字... 详细信息

数字闭环石英挠性加速度计可以有效地解决传统模拟式加速度计在模数转换中引起的精度损失问题,而数字式加速度计的精度主要取决于差动电容检测环节。阐述了数字式加速度计的工作原理,重点设计了差动电容检测方法,利用单载波调制与数字自相关解调技术构建完整的数字式加速度计信号检测方案。搭建数字式加速度计原理样机,对其进行0 g/±1 g稳定性测试,得到数字式加速度计0 g/±1 g零偏稳定性分别为17.595 0μg、19.363 7μg、20.715 3μg。测试结果表明,当前的数字式加速度计精度达到了模拟式加速度计精度量级,有效验证了该方案的可行性及正确性。

关键词：自相关检测加速度计数字闭环差动电容调制解调