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Dynamic patterns of the translatome in a hybrid triplet show translational fractionation of the maize subgenomes

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The Crop Journal 2022年第1期10卷 36-46页

作者： Wanchao Zhu Sijia Chen Tifu Zhang Jia Qian zi luo Han Zhao Yirong Zhang Lin Li National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina Jiangsu Provincial Key Laboratory of Agrobiology Institute of Germplasm Resources and BiotechnologyJiangsu Academy of Agricultural SciencesNanjing 210014JiangsuChina Key Laboratory of Crop Heterosis and Utilization(MOE)and State Key Laboratory for Agrobiotechnology Key Laboratory of Crop Genomics and Genetic Improvement(MOA)Beijing Key Laboratory of Crop Genetic ImprovementChina Agricultural UniversityBeijing 100193China

Heterosis,the phenomenon in which hybrids outperform their parents,has been utilized in maize(Zea mays L.)for over 100 *** provide a more complete understanding of heterosis,we collected a comprehensive transcriptome and translatome dataset on seedling leaves for B73,Mo17,and their F1 hybrid,which provided a dynamic landscape of transcriptomic and translatomic variation in *** additivity accounted for a large proportion of variation at two omics-levels,an elevated nonadditive effect was observed in the translatome,especially in the translated subgenome maize1 genes,and the genes that switched from additivity in the transcriptome to nonadditivity in the translatome were significantly enriched in the subgenome *** genes with allele-specific expression and translation show dramatic regulatory switches between the transcriptome and translatome,and partial genes with allele-specific translation underlying regulatory mechanism also exhibited subgenome ***,we found the translated isoforms show different expression patterns compared with transcriptome and more genes changed their dominant isoforms during the genetic flow from parents to the hybrid at the translatome *** translated genes with switched dominant isoforms significantly biased to the subgenome maize2 while genes with conserved dominant isoforms significantly enriched in subgenome ***,the dynamic changed patterns in translatome across hybrid and parental lines show translational fractionation of the maize subgenomes,which may be associated with heterosis in maize and provides a potential theoretical basis for breeding.

关键词： Heterosis Translatome Ribosome-sequencing(Ribo-seq) Subgenomes Maize

Site-specifically Hydrolytic Cleavage of Oxidized Insulin B Chain With Cu(II) Ion

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Chinese Chemical Letters 2000年第11期11卷 951-954页

作者： xue Mei luo Wei Jiang HE Yu ZHANG zi Jian GUO Long Gen ZHU State Key Laboratory of Coordination Chemistry Nanjing UniversityNanjing 210093

Electrospray mass spectrometry investigation shows that denatured oxidized insulin B chain can be selectively cleaved by simple Cu(II) ion and the site of cleavage is at Gly8 - Ser9 bond which is second amide bond lef... 详细信息

关键词： Cu2+ Electrospray mass spectrometry Hydrolytic cleavage Oxidized insulin B chain

Insensitive Acetylcholine Receptor Conferring Resistance of Plutella xylostella to Nereistoxin Insecticides

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Agricultural Sciences in China 2008年第7期7卷 847-852页

作者： CHENG luo-gen YU Guang CHEN zi-hao LI Zhong-yin Jiangsu Key Laboratory for Biodiversity and Biotechnology College of Life Science Nanjing Normal University Nanjing 210097 P.R. China The Pre-Clinical Medicine College Nanjing University of Traditional Chinese Medicine Nanjing 210029 P.R. China Institute of Plant Protection Guizhou Academy of Agricultural Sciences Guiyang 550006 P.R.China

The combinative rate measurement of （3-[I125] iodotyrosyl） α-bungarotoxin was applied in the analysis of the relation between nerve acetylcholine receptor and three types of insecticide resistance in diamondback moth, Plutella xylostella （L.）. In the dimehypo-resistant strain and in the cartap-resistant strain, the nerve acetylcholine receptor showed the remarkable insensitivity to dimehypo and cartap, of which the binding rate to ligand was approximately 66 and 60%, respectively, of the susceptible strain. The sensitivity to deltamethrin in the deltamethrin-resistant strain did not show visible change. These results indicated that the decline in the sensitivity of nerve acetylcholine receptor to insecticide might be a potential mechanism to nereistoxin insecticides resistance in the diamondback moth.

关键词： diamondback moth dimehypo cartap deltamethrin nerve acetylcholine receptor insecticide resistance

Preparation and properties of hot-deformed magnets processed from nanocrystalline/amorphous Nd-Fe-B powders

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Rare Metals 2021年第8期40卷 2033-2039页

作者： Peng Zhao Yang luo Dun-Bo Yu Hai-Jun Peng Wen-Long Yan zi-Long Wang xin-Yuan Bai National Engineering Research Center for Rare Earth Materials General Research Institute for Nonferrous MetalsBeijing100088China Grirem Advanced Materials Co. LtdBeijing100088China

The hot-deformed magnets processed from nanocrystalline/amorphous Nd-Fe-B powders were preparedunderdifferenthot-pressingtemperatures(600-750℃,at intervals of 25℃)by the self-made hotpressing *** microstructure and magnetic properties of hot-deformed magnets prepared at different temperatures were also *** the temperature is above 650℃,the density of magnet reaches 7.5 g·cm^(-3).The optimum magnetic properties of magnetic induction intensity of B_(r)=1.3 T,optimum energy product of(BH)_(max)=282.5 kJ·m^(-3),intrinsiccoercivityof H_(cj)=1130.0 kA·m-1 of hot-deformed magnets are obtained at hot-pressing temperature of 650℃.x-ray diffractometer pattern shows that the(00 L)texture has been *** the microstructural characteristic,on the one hand,the good magnetic performance is attributed to the fine platelet-like grains with an average length of 410-440 nm at the hot-pressing temperature range from625 to 675℃.On the other hand,the unaligned coarse grains are observed in all the *** the areal fraction of those is gradually increasing with the rise of the hot-pressing temperature,which tends to deteriorate the magnetic *** composition map shows the accumulation of Nd/Pr-rich phase in the coarse grains’region.

关键词： Nanocrystalline/amorphous powders Hotpressing temperature Hot-deformed Magnets Magnetic properties Microstructure

Lipotoxic hepatocyte-derived exosomal miR-1297 promotes hepatic stellate cell activation through the PTEN signaling pathway in metabolic-associated fatty liver disease

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World Journal of Gastroenterology 2021年第14期27卷 1419-1434页

作者： xin luo Sheng-Zheng luo zi-xin xu Cui Zhou Zheng-Hong Li xiao-Yan Zhou Ming-Yi xu Department of Gastroenterology Shanghai General HospitalShanghai 200080China

BACKGROUND Exosomes play an important role in metabolic-associated fatty liver disease(MAFLD),but the mechanism by which exosomes participate in MAFLD still remain *** To figure out the function of lipotoxic exosomal miR-1297 in *** MicroRNA sequencing was used to detect differentially expressed miRNAs(DEmiR)in lipotoxic exosomes derived from primary *** tools were applied to analyze the target genes and pathways regulated by the *** real-time PCR(qPCR)was conducted for the verification of ***,western blot,immunofluorescence staining and ethynyl-20-deoxyuridine assay were used to evaluate the function of lipotoxic exosomal miR-1297 on hepatic stellate cells(Lx2 cells).A luciferase reporter experiment was performed to confirm the relationship of miR-1297 and its target gene *** MicroRNA sequencing revealed that there were 61 exosomal DE-miRs(P2 from palmitic acid treated primary hepatocytes compared with the vehicle control ***-1297 was the most highly upregulated according to the microRNA *** tools showed a variety of target genes and pathways regulated by these DE-miRs were related to liver ***-1297 was overexpressed in exosomes derived from lipotoxic hepatocytes by *** promoting genes(α-SMA,PCNA)were altered in Lx2 cells after miR-1297 overexpression or miR-1297-rich lipotoxic exosome incubation via qPCR and western blot *** staining and ethynyl-20-deoxyuridine staining demonstrated that the activation and proliferation of Lx2 cells were also promoted after the above *** was found to be the target gene of miR-1297 and knocking down PTEN contributed to the activation and proliferation of Lx2 cells via modulating the PI3K/AKT signaling *** miR-1297 was overexpressed in exosomes derived from lipotoxic *** lipotoxic hepatocyte-derived exosomal miR-1297 could promot

关键词： Metabolic-associated fatty liver disease miRNA-1297 Exosome Hepatic stellate cell PTEN Liver fibrosis

High energy storage capability of perovskite relaxor ferroelectrics via hierarchical optimization

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Rare Metals 2022年第3期41卷 730-744页

作者： Min-Hao Zhang Jun-Lei Qi Yi-Qian Liu Shun Lan zi-xi luo Hao Pan Yuan-Hua Lin State Key Laboratory of New Ceramics and Fine Processing School of Materials Science and EngineeringTsinghua UniversityBeijing 100084China Innovation Team for New High Volumetric Efﬁcient MLCC Materials and Devices Foshan(Southern China)Institute for New MaterialsFoshan 528000China Division of Physics and Applied Physics School of Physical and Mathematical SciencesNanyang Technological UniversitySingapore 639798Singapore

Ultrafast charge/discharge process and ultrahigh power density enable dielectrics essential components in modern electrical and electronic devices, especially in pulse power systems. However, in recent years, the energy storage performances of present dielectrics are increasingly unable to satisfy the growing demand for miniaturization and integration, which stimulates further researches on dielectrics with higher energy density and *** various inorganic dielectrics, perovskite relaxor ferroelectrics are recognized as promising candidates for energy storage applications, with high permittivity and relatively high efficiency. Here, we focus on recent progress and achievements on optimizing perovskite relaxor ferroelectrics toward better energy storage capability through hierarchical design. The principles and key parameters of dielectric energy storage, together with the definition of majority types of dielectrics, are introduced at first. Strategies within various scales include domain, grain size, orientation, and composite engineering are summarized. The existing challenges are presented and future prospects are proposed in the end, with the background of both academic explorations and industrial applications.

关键词： Dielectric Energy storage Capacitance Perovskite Relaxor ferroelectric

Inhibition of α5 GABAA receptors has preventive but not therapeutic effects on isoflurane-induced memory impairment in aged rats

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Neural Regeneration Research 2019年第6期14卷 1029-1036页

作者： zi-Fang Zhao Lei Du Teng Gao Lin Bao Yuan luo Yi-Qing Yin Yong-An Wang Department of Anesthesiology China-Japan Friendship Hospital Department of Radiology China-Japan Friendship Hospital Department of Anesthesiology Beijing Shijitan Hospital Affiliated to Capital Medical University Institute of Pharmacology and Toxicology Academy of Military Medical Sciences

The α5 subunit-containing gamma-amino butyric acid type A receptors(α5 GABAARs) are a distinct subpopulation that are specifically distributed in the mammalian hippocampus and also mediate tonic inhibitory currents in hippocampal neurons. These tonic currents can be enhanced by low-dose isoflurane, which is associated with learning and memory impairment. Inverse agonists of α5 GABAARs, such as L-655,708, are able to reverse the short-term memory deficit caused by low-dose isoflurane in young animals. However, whether these negative allosteric modulators have the same effects on aged rats remains unclear. In the present study, we mainly investigated the effects of L-655,708 on low-dose(1.3%) isoflurane-induced learning and memory impairment in elderly rats. Young(3-month-old) and aged(24-month-old) Wistar rats were randomly assigned to receive L-655,708 0.5 hour before or 23.5 hours after 1.3% isoflurane *** Morris Water Maze tests demonstrated that L-655,708 injected before or after anesthesia could reverse the memory deficit in young rats. But in aged rats, application of L-655,708 only before anesthesia showed similar effects. Reverse transcription-polymerase chain reaction showed that low-dose isoflurane decreased the mRNA expression of α5 GABAARs in aging hippocampal neurons but increased that in young animals. These findings indicate that L-655,708 prevented but could not reverse 1.3% isoflurane-induced spatial learning and memory impairment in aged Wistar rats. All experimental procedures and protocols were approved by the Experimental Animal Ethics Committee of Academy of Military Medical Science of China(approval No. NBCDSER-IACUC-2015128) in December 2015.

关键词： isoflurane postoperative cognitive dysfunction hippocampus inverse agonist α5 GABAA receptors L-655,708 aged Morris Water Maze memory impairment neural regeneration

Effect of Tillage System on Distribution of Aggregates and Organic Carbon in a Hydragric Anthrosol

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Pedosphere 2008年第5期18卷 574-581页

作者： GAO Ming luo You-Jin WANG zi-Fang TANG xiao-Hong WEI Chao-Fu College of Resources and Environment Southwest University Chongqing 400716 （China）.

The effect of different tillage systems on the size distribution of aggregates and organic carbon distribution and storage in different size aggregates in a Hydragric Anthrosol were studied in a long-term experiment in Chongqing,China. The experiment included three tillage treatments:conventional tillage with rotation of rice and winter fallow(CT-r) system,no-till and ridge culture with rotation of rice and rape(RT-rr) system,and conventional tillage with rotation of rice and rape(CT-rr) system. The results showed that the aggregates 0.02-0.25 mm in diameter accounted for the largest portion in each soil layer under all treatments. Compared with the CT-r system,in the 0-10 cm layer,the amount of aggregates > 0.02 mm was larger under the RT-rr system,but smaller under the CT-rr system. In the 0-20 cm layer,the organic carbon content of all fractions of aggregates was the highest under the RT-rr system and lowest under the CT-rr system. The total organic carbon content showed a positive linear relationship with the amount of aggregates with diameter ranging from 0.25 to 2 mm. The storage of organic carbon in all fractions of aggregates under the RT-rr system was higher than that under the CT-r system in the 0-20 cm layer,but in the 0-60 cm soil layer,there was no distinct difference. Under the CT-rr system,the storage of organic carbon in all fractions of aggregates was lower than that under the CT-r system;most of the newly lost organic carbon was from the aggregates 0.002-0.02 and 0.02-0.25 mm in diameter.

关键词： aggregates Hydragric Anthrosol organic carbon tillage system

Hot air treatment activates defense responses and induces resistance against Botrytis cinerea in strawberry fruit

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Journal of Integrative Agriculture 2016年第11期15卷 2658-2665页

作者： JIN Peng ZHENG Cong HUANG Yu-ping WANG xiao-li luo zi-sheng ZHENG Yong-hua College of Food Science and Technology Nanjing Agricultural University College of Life Science and Food Engineering Huaiyin Institute of Technology College of Biosystems Engineering and Food Science Zhejiang University

The effect of hot air（HA, 45°C, 3.5 h） treatment on reducing gray mold caused by Botrytis cinerea in strawberry fruit and the possible mechanisms were investigated. The results showed that HA treatment significantly reduced lesion diameter and enhanced activities of chitinase（CHI）, β-1,3-glucanase and phenylalanine ammonia-lyase（PAL） in strawberry fruit. Total phenolic contents were also increased by HA treatment. The activities of antioxidant enzymes including superoxide dismutase（SOD）, catalase（CAT） and ascorbate peroxidase（APx） were higher in HA treated strawberry fruit than those in control. Expression of three defense related genes such as CAT, CCR-1 allele and PLA6 was greatly induced in HA treated strawberry fruit with or without inoculation by B. cinerea. In addition, the in vitro experiment showed that HA treatment inhibited spore germination and tube growth of B. cinerea. These results suggested that HA treatment directly activated disease resistance against B. cinerea in strawberry fruit without priming response and directly inhibiting growth of B. cinerea.

关键词： strawberry hot air postharvest disease induced resistance Botrytis cinerea