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Isolation and Characterization of Radiation-resistant Lung Cancer D6-R Cell Line

Biomedical and Environmental Sciences 2008年第4期21卷 339-344页

作者： QI-CHUn WEI LI SHEn SHU ZHEnG YOnG-LIAnG zhu Ministry of Education Key Laboratory of Cancer Prevention and Intervention Zhejiang University Hangzhou 310009 Zhejiang China Department of Radiation Oncology the Second Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310009 Zhejiang China

Objective To isolate an isogenic radioresistant cancer cell line after fractioned x-ray radiation and characterize the resistant cells. Methods D6 cells were exposed to repeated x-ray irradiation, and after a total dose of 5200 cGy in 8 fractions, a radioresistant monoclone D6-R was obtained. The radiosensitivity and drug sensitivity of the novel radioresistant D6-R cells, together with their parent D6 cells, were measured using clonogenic assay and MTT assay respectively. Cell cycle distribution was analyzed by flow cytometry. Fluorescence microscopy and flow cytometry were applied for apoptosis detection Comet assay was used for the detection of DnA damage and repair. Results D6-R cells showed higher and broader initial shoulder （D0=2.08 Gy, Dq=1.64 Gy, n=2.20） than the parent D6 cells （D0=1.84 Gy, Dq=0.34 Gy, n=1.20）. They were 1.65-fold more radioresistant than D6 cells in terms of SF2 （63% vs 38%） and were more resistant to ADM （3.15-fold） and 5-FU （3.86-fold） as compared with the latter. It was found that D6-R cells had higher fractions of cells in S phase （53.4% vs 37.8%） and lower fractions of cells in G1 （44.1% vs 57.2%） and G2-M phase （2.5% vs 5%）. There was no difference in radiation-induced apoptosis between D6-R and D6 cells. D6-R cells showed less initial DnA damage and increased capacity in DnA repair after irradiation, as compared with the parent cells. Conclusions D6-R cells have been isolated by exposing the parental D6 cells to repeated irradiation. The difference in cell cycle pattern together with the induction and repair of DnA damage might, at least partially, explain the mechanism of the radioresistance.

关键词： Radiosensitivity Chemosensitivity Cell cycle Apoptosis DnA damage DnA repair

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Variation characteristics of heavy metals and nutrients in the core sediments of Taihu Lake and their pollution history

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Science China Earth Sciences 2006年第Z1期49卷 82-91页

作者： LIU Enfeng SHEn Ji LIU x.ngqi zhu Yux.n WAnG Sumin nanjing Institute of Geography & Limnology Chinese Academy of Sciences Nanjing 210008 China Nanjing Institute of Geography & Limnology Chinese Academy of Sciences Nanjing 210008 China Nanjing Institute of Geography & Limnology Chinese Academy of Sciences Nanjing 210008 China Nanjing Institute of Geography & Limnology Chinese Academy of Sciences Nanjing 210008 China Nanjing Institute of Geography & Limnology Chinese Academy of Sciences Nanjing 210008 China

The sedimentary environment change, trophic evolution and heavy metals pollution history of the northern Taihu Lake in the last 100 years are studied according to the sedimentary geochemical proxies of the core sediments, such as grain size, nutrients, heavy metals, diatom, etc. The nutrients in the sediments depended mainly on the lake internal circulation and the heavy metals were from natural geogenic sources before the 1920s, which were not influenced by human activities generally, and grain size was one of the key factors influencing heavy metals content in the *** alternation of manner and strength of human activities in Taihu Lake catchment before and after the 1920s made the lake sediments coarser, and hence heavy metals and TP content decreased contrasted with that before the 1920s. TP content in sediments and water increased from the 1950s to late 1970s due to anthropogenic pollutants discharge, and the lake belonging to mesotrophic *** and TOC content and C/n ratio increased due to the increasing external pollutants into Taihu Lake by human activities, TP content also increased obviously in water and sediments, and the diatom association was dominated by eutrophic species since the late 1970s, indicating the eutrophication state of Taihu Lake in this period. Meanwhile the increasing in heavy metals content, such as Cu, Mn,ni, Pb and Zn, and their proportion of valid fractions in the sediments indicates that they all result from human pollutants since the late 1970s. The heavy metals in the surface sediments have certain potential biological toxicity due to the higher SEM/AVS ratio.

关键词： sediments, heavy metals, nutrients, human activities, pollution history, Taihu Lake.

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A new and efficient method for the synthesis of isoquinoline-3-carboxylate

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Chinese Chemical Letters 2008年第3期19卷 253-255页

作者： xiang Wei Liao Bao He Guan Zhan zhu Liu Institute of Materia Medica Chinese Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100050. PR China

An isoquinoline-3-carboxylate compound 3 was obtained with a moderate yield of 40% when n-acetyl-（3′-hydroxy-4′- methoxy-5′-methyl）phenylalanine methyl ester 1 was refluxed in HMTA/TFA. However, the anticipated product n-acetyl-（3′- hydroxy-4′-methoxy-5′-methyl-6′-formyl）phenylalanine methyl ester 2 could not be found. The possible mechanism was discussed in this article.

关键词： Isoquinolinc-3-carboxylate Duff reaction n-Acetyl-phenylalanine methyl ester

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辽东湾地区孔隙演化的机理

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地球科学（中国地质大学学报） 2007年第3期32卷 366-372页

作者：蒋恕蔡东升朱筱敏朱光辉胡孝林中海石油研究中心北京100027 中海石油有限公司湛江分公司广州湛江524057 中国石油大学资源与信息学院北京102249

孔隙演化机理对研究中深层储层控制因素、预测中深层有利储层及中深层隐蔽油气藏勘探具有重大意义.通过辽东湾地区的辽中凹陷、辽西凸起及辽西凹陷孔隙演化分析表明,辽东湾地区凹陷和凸起的孔隙演化阶段是一致的,即在总体降低的背景中存... 详细信息

孔隙演化机理对研究中深层储层控制因素、预测中深层有利储层及中深层隐蔽油气藏勘探具有重大意义.通过辽东湾地区的辽中凹陷、辽西凸起及辽西凹陷孔隙演化分析表明,辽东湾地区凹陷和凸起的孔隙演化阶段是一致的,即在总体降低的背景中存在4个次生孔隙发育带,所不同的是凹陷的次生孔隙发育带相对凸起深,次生孔隙发育带与成岩演化、有机质的演化、粘土矿物的转化、超压、油气的早期充注密切相关.探讨了孔隙演化过程中孔隙度降低和次生孔隙发育的机理,认为辽东湾地区4个次生孔隙发育带的形成主要源于有机酸对碳酸盐胶结物、长石、岩屑的溶蚀以及超压和早期油气充注对孔隙的保护.

关键词：辽东湾地区孔隙演化次生孔隙演化机理成岩有机质演化早期油气充注超压

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Precipitation behavior of Ti in high strength steels

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Journal of Central South University 2017年第12期24卷 2767-2772页

作者： WU Si-wei LIU Zhen-yu ZHOU xiao-guang YAnG Hao WAnG Guo-dong State Key Laboratory of Rolling Technology and Automation Northeastern University Shenyang 110819 China

Precipitation behavior of Ti in high strength steels was investigated by means of the equilibrium solid solubility theory. The contributions of Ti content to yield strength were calculated. The calculated results were verified by the hot rolling experiment for C–Mn steel and C–Mn–Ti micro alloyed steel, respectively. The research results show that the precipitates are mainly Ti n at the higher temperature. With the decreasing temperature, the proportion of Ti C in precipitates increases gradually. When the temperature drops to 800 °C, Ti C will become predominant for the precipitation of Ti. When Ti content is less than 0.014%(mass fraction), Ti has little influence on the yield strength. When Ti content is in the range of 0.014%–0.03%(mass fraction), the yield strength of Ti micro alloyed steel is greatly increased, which leads to instability of the mechanical properties of the steel. Therefore, the design of Ti content in high strength steels should avoid this Ti content range. When Ti content is higher than 0.03%, the yield strength increases stably. In this experiment, when added Ti content was controlled in the range of 0.03%–0.05%, the contribution to the yield strength of Ti micro alloyed steel can reach about 92.44 MPa.

关键词： precipitation strengthening solid solubility Ti(C, n) yield strength high strength steel

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novel expanded porphyrinoids with multiple-inner-ring-fusion and/or tunable aromaticity

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Chinese Chemical Letters 2019年第11期30卷 1895-1902页

作者： Wei Miao Zhaoyang zhu Zhongx.n Li Erhong Hao Lijuan Jiao Laboratory of Functional Molecular Solids Ministry of EducationSchool of Chemistry and Materials ScienceAnhui Normal University

novel expanded porphyrinoids with advanced structure features(such as multiple-inner-ring-fusion)have a wide range of benefits(such as multi-metal coordination and facile tunable aromaticity)not offered by their normal porphyrin analogues,and have found wide applications as sensors,fluorescent probes,novel ligands and functionalized nIR organic dyes in various research ***,the structures of these expanded porphyrinoids are scarce due to their limited synthetic ***,we summarized the lately reported efficient synthesis of novel expanded porphyrinoids with multipleinner-ring-fusion(up to six-inner-ring-fusion)and smaragdyrins with tunable *** synthesis is either based on an oxidative ring cyclization on linear/macrocyclic oligopyrroles containing n-confused pyrrole unit(s)or a straightforward double SnAr reaction on readily available 3,5-dibromoBODIPY,respectively.

关键词： Porphyrinoids Oligopyrroles BODIPYs Aromaticity Macrocycles

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乌司他丁联合连续性血液净化治疗重症脓毒症的效果及对微循环指标、炎症指标及预后的影响

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黑龙江医药科学 2024年第1期47卷 145-147页

作者：徐飞方朱义洪薛细松莆田市第一医院急诊科福建莆田351100

目的:探讨重症脓毒症患者采用乌司他丁联合连续性血液净化(CBP)治疗的效果及对其微循环指标、炎症指标及预后产生的影响。方法:选取2021年12月至2023年5月莆田市第一医院ICU收治的重症脓毒症102例患者,根据其入院编号顺序按照1:1比例分... 详细信息

目的:探讨重症脓毒症患者采用乌司他丁联合连续性血液净化(CBP)治疗的效果及对其微循环指标、炎症指标及预后产生的影响。方法:选取2021年12月至2023年5月莆田市第一医院ICU收治的重症脓毒症102例患者,根据其入院编号顺序按照1:1比例分至两组中,即对照组(n=51,予以单纯CBP治疗)与观察组(n=51,予以CBP+乌司他丁治疗),对两组治疗效果、微循环指标、炎症指标及预后情况进行综合对比分析。结果:观察组94.12%和对照组76.47%的总有效率比较,前者更具优势(P<0.05);两组治疗前后微循环指标差异显著,PVD、PPV及MFI提升显著,TVD下降显著,而观察组以上指标提升/下降幅度大于对照组,差异有统计学意义(P<0.05);两组治疗前后血清炎性因子水平差异明显,PCT、CRP及IL-10可见显著下降,但组间对比发现,观察组以上各指标更低(P<0.05);与对照组比较,观察组在机械通气、ICU住院及总住院时间方面更占优势,且28d病死率更低(P<0.05)。结论:乌司他丁联合CBP治疗重症脓毒症可提高治疗效果,不仅可有效改善机体微循环,还能减轻机体炎症反应,改善患者预后。

关键词：乌司他丁重症脓毒症微循环指标炎症指标预后

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上海市中小学校心理健康服务现状与影响因素调查

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海军军医大学学报 2024年第7期45卷 821-830页

作者：王丽霞柏涌海张泽堃朱园飞王一浩孙铭赛庞维国上海市教师教育学院上海200234 海军军医大学(第二军医大学)第二附属医院医学心理科上海200003 华东师范大学心理与认知科学学院上海200062

目的调查上海市中小学校心理健康服务现状和影响因素,为加强学校心理健康师资队伍建设、科学有效地开展儿童青少年心理健康服务工作提供依据。方法采用自行设计的中小学校心理健康服务问卷对上海市14个区的422名心理健康教师进行调查。... 详细信息

目的调查上海市中小学校心理健康服务现状和影响因素,为加强学校心理健康师资队伍建设、科学有效地开展儿童青少年心理健康服务工作提供依据。方法采用自行设计的中小学校心理健康服务问卷对上海市14个区的422名心理健康教师进行调查。采用Pearson χ^(2)检验和独立样本t检验比较不同学历心理健康教师在学校心理健康服务情况和影响因素评估上的差异,构建结构方程模型分析学生获得心理健康服务的影响因素。结果422名调查对象的毕业专业主要有应用心理学(124人,29.38%)、教育学(117人,27.73%)、发展与教育心理学(65人,15.40%)等。心理健康教师认为学生中最常见的心理健康问题依次是社会/人际问题、家庭问题,焦虑、压力或学校恐惧,以及适应问题。与学校签订协议/合作提供心理健康服务的组织主要有区心理健康中心(376所,89.10%)、社区健康中心(155所,36.73%)、当地医院(106所,25.12%)。在学校提供的心理健康服务、学校提供的预防和早期干预项目、学校收集和留存的数据、学生获得心理健康服务影响因素等方面的一些题目上,硕士学历心理健康教师报告的比例或评分高于本科学历心理健康教师(均P<0.05)。近3年对心理健康服务需求(328所,77.73%)、服务的学生数(314所,74.41%)增加,而心理健康服务的资金(212所,50.24%)、学校心理健康服务体系人员的数量(226所,53.55%)没有变化。学生获得心理健康服务影响因素的结构方程模型包含学校、家庭和社会3个维度,模型拟合指数良好(χ^(2)/df=2.708,近似误差均方根为0.064,规范拟合指数为0.947,拟合优度指数为0.946,调整拟合优度指数为0.912,比较拟合指数为0.965,相对拟合指数为0.926,塔克-刘易斯指数为0.952,增值拟合指数为0.966,均方根残差为0.046)。结论上海市中小学校心理健康工作成效显著,未来仍须加强专职心理健康教师职后培训,通过家校社协同、医教结合等多元化方式保障资源供给,并且要保障心理健康专项经费。

关键词：心理健康心理健康教师职后培训中小学校

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Inhomogeneous Deformation of Multilayered Roll-Bonded Brass/Cu Composites

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Acta Metallurgica Sinica(English Letters) 2015年第5期28卷 600-607页

作者： nan Jia Ming-Wei zhu Yi-Ran Zheng Tong He xiang Zhao Key Laboratory for Anisotropy and Texture of Materials (ATM) Northeastern University Shenyang national Laboratory for Materials Science Institute of Metal Research Chinese Academy of Sciences Research Institute Northeastern University

The deformation gradients in multilayered hot roll-bonded composite materials incorporating two dissimilar face-centered cubic metals, i.e., brass and Cu, were investigated by characterizing the deformation microstructure, hardness and texture at different thickness positions of the composites. For the constitutive metals, the center part of each metal sheet forms the substructure containing coarse grains, while the ultrafine grains and significant shear banding form in the outer part. As deformation increases, the cross-interface shear occurs in the Cu sheet of the composites. Then, grain fragmentation, shear banding and dynamic recovery become the main factors that influence hardness of the metal. Moreover, in the co-deformed composites, the interface between brass and Cu plays a role in texture developments of the individual metals.

关键词： Deformation Multilayers Brass/Cu composites Texture

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Variation of bacterial community associated with Phaeodactylum tricornutum in response to different inorganic nitrogen concentrations

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Acta Oceanologica Sinica 2018年第12期37卷 118-128页

作者： SHI Feng WEI xiaoxue FEnG Jianfeng SUn Yingxue zhu Lin Key Laboratory of Pollution Process and Environmental Criteria of Ministry of Education and Tianjin Key Laboratory of Environmental Remediation and Pollution Control College of Environmental Science and Engineering Nankai University

Specific bacterial communities interact with phytoplankton in laboratory algal cultures. These communities influence phytoplankton physiology and metabolism by transforming and exchanging phytoplankton-derived organic matter. Functional bacterial groups may participate in various critical nutrients fluxes within these associations, including nitrogen(n) metabolism. However, it is unclear how bacterial communities and the associated algae respond to changes of phycosphere n conditions. This response may have far-reaching implications for global nutrient cycling, algal bloom formation, and ecosystem function. Here, we identified changes in the bacterial communities associated with Phaeodactylum tricornutum when co-cultured with different forms and concentrations of n based on the Illumina HiSeq sequencing of 16 S rRnA *** analysis identified Proteobacteria and Bacteroidetes as the dominant phyla, accounting for 99.5% of all sequences. Importantly, bacterial abundance and community structure were more affected by algal abundance than by the form or concentration of inorganic n. The relative abundance of three gammaproteobacterial genera(Marinobacter, Algiphilus and Methylophaga) markedly increased in n-deficient cultures. Thus, some bacterial groups may play a role in the regulation of n metabolism when co-cultured with ***.

关键词： Phaeodactylum tricornutum nitrogen concentrations nitrogen forms bacterial diversity community structure Gammaproteobacteria

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