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纳曲酮治疗酒依赖患者的安慰剂双盲对照研究

中国药物依赖性杂志 2009年第4期18卷 325-330页

作者：原伟武新汶卢怀云由炜米国琳王帅王锋刘希俊朱宝俊唐济生山东省精神卫生中心济南250014 山东省疾病预防控制中心济南250014 山东省枣庄市立二院枣庄277000 临沂市第四人民医院临沂276000 泰安市精神卫生中心泰安271000

目的:探讨纳曲酮治疗酒依赖患者的疗效与安全性。方法:采用纳曲酮和安慰剂双盲对照研究方法,将68例门诊酒依赖患者随机入组,治疗12周,在不同治疗时段采用密执根酒精依赖调查表(MAST)、饮酒渴求度、饮酒量调查表、SCL-90、不良事件、血... 详细信息

目的:探讨纳曲酮治疗酒依赖患者的疗效与安全性。方法:采用纳曲酮和安慰剂双盲对照研究方法,将68例门诊酒依赖患者随机入组,治疗12周,在不同治疗时段采用密执根酒精依赖调查表(MAST)、饮酒渴求度、饮酒量调查表、SCL-90、不良事件、血生化、心电图等各项工具进行测试。结果:观察期内纳曲酮组可视渴求量表在治疗的第10周及第12周分别为1.77±s1.71、1.52±s1.61,与安慰剂组比较差异有显著性(P值均0.05);不良事件纪录显示纳曲酮比较常见的不良反应主要为消化道反应(9/34)和乏力、头疼等神经系统副作用(3/34)。结论:纳曲酮能有效降低酒依赖患者的心理渴求、酒精消耗和复饮风险。同时也能缓解酗酒导致的焦虑、恐怖、偏执及躯体化症状。纳曲酮的不良反应程度较轻,持续时间较短,患者多可耐受,是一种安全有效的酒依赖治疗药物。

关键词：纳曲酮酒精诱发性障碍复发

Phosphatidylserine-Specific Phospholipase A1 is the Critical Bridge for Hepatitis C Virus Assembly

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Virologica Sinica 2019年第5期34卷 521-537页

作者： Qi Yang Min Guo Yuan Zhou Xue Hu Yun Wang Chunchen wu Min Yang Rongjuan Pei xinwen Chen Jizheng Chen Department of Gastroenterology Guangzhou Women and Children's Medical CenterGuangzhou 510623China State Key Laboratory of Virology Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China School of Life Science and Technology China Pharmaceutical UniversityNanjing 210009China

The phosphatidylserine-specific phospholipase A1(PLA1A)is an essential host factor in hepatitis C virus(HCV)*** this study,we mapped the E2,NS2 and NS5A involved in PLA1A interaction to their lumenal domains and membranous parts,through which they form oligomeric protein complexes to participate in HCV *** regions of PLA1A were involved in their interaction and complex ***,the results represented structures with PLA1A and E2 in closer proximity than NS2 and NS5A,and strongly suggest PLA1 A-E2,s physical interaction in ***,we mapped the NS5A sequence which participated in PLA1A interaction with the C-terminus of domain ***,these amino acids in the sequence are also essential for viral RNA *** experiments revealed that these four proteins interact with each ***,PLA1A expression levels were elevated in livers from HCV-infected *** conclusion,we exposed the structural determinants of PLA1A,E2,NS2 and NS5A proteins which were important for HCV assembly and provided a detailed characterization of PLA1A in HCV assembly.

关键词： Phosphatidylserine-specific phospholipase A1(PLA1 A) HCV assembly Viral RNA replication

Enhanced host immune responses in presence of HCV facilitate HBV clearance in coinfection

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Virologica Sinica 2022年第3期37卷 408-417页

作者： Shuhui Liu Kaitao Zhao Xi Su Xiaoxiao Gao Yongxuan Yao Ranran Kong Yun Wang Chunchen wu Mengji Lu xinwen Chen Rongjuan Pei Center for Biosafety Mega-Science State Key Laboratory of VirologyWuhan Institute of VirologyChinese Academy of SciencesWuhan430071China University of Chinese Academy of Sciences Beijing100049China Department of Thoracic Surgery The Second Affiliated Hospital of Medical SchoolXi'an Jiaotong UniversityXi'an710004China Department of Medicine Icahn School of Medicine at Mount SinaiNew York10029USA Institute of Virology University Hospital EssenUniversity of Duisburg-EssenEssen45147Germany Guangzhou Laboratory Guangzhou510005China Current address:Division of Infectious Diseases Department of MedicineIcahn School of Medicine at Mount SinaiNew York10029USA Current address:State Key Laboratory of Virology and Hubei Province Key Laboratory of Allergy and Immunology School of Basic Medical SciencesWuhan UniversityWuhan430071China Current address:Henan Mental Hospital the Second Affiliated Hospital of Xinxiang Medical UniversityXinxiang453002China Current address:Joint Center of Translational Precision Medicine Guangzhou Institute of PediatricsGuangzhou Women and Children Medical CenterGuangzhou510623China Current address:Department of Laboratory Medicine Maternal and Child Health Hospital of Hubei ProvinceTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China

Hepatitis B virus(HBV)/Hepatitis C virus(HCV)coinfection is frequently observed because of the common infection *** the reciprocal inhibition exerted by HBV and HCV genomes,the coinfection of HBV and HCV is associated with more severe forms of liver ***,the complexity of viral interference and underlying pathological mechanism is still *** the demonstration of absence of direct viral interplay,some in vitro studies suggest the indirect effects of viral-host interaction on viral dominance ***,we comprehensively investigated the viral replication and host immune responses which might mediate the interference between viruses in HBV/HCV coinfected Huh7-NTCP cells and immunocompetent HCV human receptors transgenic ICR *** found that presence of HCV significantly inhibited HBV replication in vitro and in vivo irrespective of the coinfection order,while HBV did not affect HCV *** alteration was coincidently reproduced in coinfected *** addition to the participation of innate immune response,an involvement of HCV in up-regulating HBV-specific immune responses was described to facilitate HBV *** systems partially recapitulate HBV/HCV coinfection and unveil the uncharacterized adaptive anti-viral immune responses during coinfection,which renews the knowledge on the nature of indirect viral interaction during HBV/HCV coinfection.

关键词： Hepatitis B virus(HBV) Hepatitis C virus(HCV) Coinfection Viral-host interaction Immunocompetent mouse model Adaptive immune responses

Hepatitis B virus is degraded by autophagosome-lysosome fusion mediated by Rab7 and related components

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Protein & Cell 2019年第1期10卷 60-66页

作者： Yong Lin Chunchen wu Xueyu Wang Thekla Kemper Anthony Squire Matthias Gunzer Jiming Zhang xinwen Chen Mengji Lu Institute of Virology University Hospital EssenUniversity of Duisburg-Essen45117 EssenGermany State Key Laboratory of Virology Wuhan Institute of VirologyChinese Academy of ScienceWuhan 430071China Institute for Experimental Immunology and Imaging University Hospital EssenUniversity Duisburg-Essen45117 EssenGermany Department of Infectious Diseases Huashan HospitalFudan UniversityShanghai 200040China

Dear Editor,With an estimated 240 million chronically infected people worldwide,hepatitis B virus(HBV)infection is a major public health problem(Schweitzer et al.,2015).Despite more than 30 years of in tense research,... 详细信息

关键词： Editor people HBV

Complementation of Wild-Type and Drug-Resistant Hepatitis B Virus Genomes to Maintain Viral Replication and Rescue Virion Production under Nucleos(t)ide Analogs

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Virologica Sinica 2019年第4期34卷 377-385页

作者： Chunchen wu Baolin Li Xiaoyong Zhang Kaitao Zhao Yingshan Chen Yifei Yuan Yan Liu Rongjuan Chen Dongping Xu xinwen Chen Mengji Lu Department of Laboratory Medicine Maternal and Child Health Hospital of Hubei ProvinceTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430070China State Key Lab of Virology Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China Institute of Virology University Hospital of Essen45122 EssenGermany University of Chinese Academy of Sciences Beijing 100049China Institute of Infectious Diseases and Liver Failure Research Center Beijing 302 HospitalBeijing 100039China

As the open reading frames of hepatitis B virus(HBV)genomes are overlapping,resistance mutations(MTs)in HBV polymerase may result in stop codon MTs in hepatitis B surface proteins,which are usually detected as a mixed population with wild-type(WT)*** question was raised how the coexistence of nucleos(t)ide analogs(NAs)resistance MTs and WT sequences affects HBV *** the present study,HBV genomes with frequently detected reverse transcriptase(RT)/surface truncation MTs,rtA181T/sW172^*,rtV191I/sW182^*and rtM204I/sW196^*,were phenotypically characterized alone or together with their WT counterparts in different ratios by transient transfection in the absence or presence of *** the absence of Nas,RT/surface truncation MTs impaired the expression and secretion of HBV surface proteins,and had a dose-dependent negative effect on WT HBV virion ***,in the presence of Nas,coexistence of MTs with WT maintained viral replication,and the presence of WT was able to rescue the production of MT HBV *** findings reveal that complementation of WT and MT HBV genomes is highly effective under drug treatment.

关键词： Hepatitis B virus(HBV) Viral replication Nucleos(t)ide analogs resistance mutations Surface truncation mutation

Productive HBV infection of well-differentiated, hNTCP-expressing human hepatoma-derived(Huh7) cells

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Virologica Sinica 2017年第6期32卷 465-475页

作者： Ming Zhou Kaitao Zhao Yongxuan Yao Yifei Yuan Rongjuan Pei Yun Wang Jizheng Chen Xue Hu Yuan Zhou xinwen Chen Chunchen wu State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Shenzhen Xenotransplantation Research and Development Center State and Local Joint Cancer Genome Clinical Application of Key Technology Laboratory Shenzhen Second People's Hospital First Affiliated Hospital of Shenzhen University Institute of Immunology Zhongshan School of Medicine Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology Sun Yat-sen University

Feasible and effective cell models for hepatitis B virus(HBV) infection are required for investigating the complete lifecycle of this virus, including the early steps of viral entry. Resistance to dimethyl sulfoxide/polyethylene glycol(DMSO/PEG), h NTCP expression, and a differentiated state are the limiting factors for successful HBV infection models. In the present study, we used a hepatoma cell line(Hu7^(hDNTCPh)) to overcome these limiting factors so that it exhibits excellent susceptibility to HBV infection. To achieve this goal, different hepatoma cell lines were tested with 2.5% DMSO/4%PEG8000, and one resistant cell line(Huh7 D) was used to construct a stable h NTCP-expressing cell line(Hu7^(hDNTCPh)) using a recombinant lentivirus system. Then, the morphological characteristics and differentiation molecular markers of Hu7^(hDNTCPh) cells with or without DMSO treatment were characterized. Finally, the susceptibility of Hu7^(hDNTCPh) cells to HBV infection was assessed. Our results showed that Huh7 D cells were resistant to 2.5% DMSO/4% PEG8000, whereas the others were not. Hu7^(hDNTCPh) cells were established to express a high level of h NTCP compared to liver extracts, and Hu7^(hDNTCPh) cells rapidly transformed into a non-dividing, well-differentiated polarized phenotype under DMSO treatment. Hu7^(hDNTCPh) cells fully supported the entire lifecycle of HBV infection. This cell culture system will be useful for the analysis of host-virus interactions, which should facilitate the discovery of antiviral drugs and vaccines.

关键词： Hepatitis B virus(HBV) Na+/taurocholate cotransporting polypeptide(NTCP) Huh7 dimethyl sulfoxide(DMSO) polyethylene glycol(PEG) susceptibility

DNA甲基化检测在肺癌早期诊断和预后判断中的应用

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中华检验医学杂志 2024年第4期47卷 371-378页

作者：张鑫雯彭诗璇杨清周佳婷张玄谢子兰龙梦乐文清央贺忆李智吴勇军南华大学衡阳医学院湘潭市第一人民医院研究生协同培养基地衡阳421001 湖南省湘潭市第一人民医院骨科湘潭411100 中南大学湘雅医院临床药理研究所长沙410000 湖南省第二人民医院普通外科长沙410000 湖南省湘潭市第一人民医院病理科湘潭411100

肺癌是癌症死亡的主要类型,大多数肺癌患者在诊断时就已为晚期,预后相当差。虽然低剂量计算机断层扫描已经进入临床作为肺癌筛查的工具,但是其假阳性率超过90%。作为研究热点的表观修饰之一,DNA甲基化在包括癌症在内的多种疾病中起着关... 详细信息

肺癌是癌症死亡的主要类型,大多数肺癌患者在诊断时就已为晚期,预后相当差。虽然低剂量计算机断层扫描已经进入临床作为肺癌筛查的工具,但是其假阳性率超过90%。作为研究热点的表观修饰之一,DNA甲基化在包括癌症在内的多种疾病中起着关键作用。抑癌基因的高甲基化和原癌基因的低甲基化是肿瘤发生发展中的重要事件。因此,DNA甲基化分析可以为肺癌的早筛、诊断、治疗和预后中提供一些有用的信息。尽管组织活检等侵入性方法仍然是肿瘤诊断和监测的金标准,但也存在取样不便等局限性。近年来,液体活检发展迅速,它可以检测原发性或转移性恶性肿瘤,并能反映肿瘤的异质性。此外,它可以微创或无创的形式进行样本收集,年老体弱患者也具有良好的耐受性。本文探讨了一些用于DNA甲基化分析的新兴技术并概述了DNA甲基化在肺癌诊疗中的应用。

关键词： DNA甲基化后成说遗传肺肿瘤液体活检

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中国粮食行业标准化进程和发展展望

粮食科技与经济 2024年第3期49卷 98-102页

作者：姜佳佳胡斌毛永荣王新文吴云季一顺安徽省粮油产品质量监督检测站安徽合肥230031

随着中国标准化引领供给侧结构性改革持续推进,粮食行业发展迈入新阶段,政府主导与市场驱动相互协调的二元新型标准体系基本建立,粮食行业标准体系覆盖粮油收购、储存、加工、运输、销售的全链条,顶层设计精简国家标准、优化行业标准、... 详细信息

随着中国标准化引领供给侧结构性改革持续推进,粮食行业发展迈入新阶段,政府主导与市场驱动相互协调的二元新型标准体系基本建立,粮食行业标准体系覆盖粮油收购、储存、加工、运输、销售的全链条,顶层设计精简国家标准、优化行业标准、彰显地方标准、培优团体标准、搞活企业标准。但粮食行业标准体系出现一些新问题,如标准结构不合理,原始创新动力不足等。面对产业发展新需求,统筹规划粮食标准体系框架、聚焦节粮减损绿色生态领域等手段是增加标准有效供给和保障能力,打造体系健全、技术创新、先进合理、开放融合的格局,引领粮食行业高质量发展,实现粮食行业现代化的重要抓手。

关键词：粮食行业标准化现状

White organic light-emitting devices fabricated by spin-coating molecular materials

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Chinese Science Bulletin 2010年第24期55卷 986-991页

作者： WANG DongDong wu ZhaoXin ZHANG xinwen JIAO Bo WANG DaWei HOU Xun School of Science Xi' an Jiaotong University Xi'an 710049 China Key Laboratory for Physical Electronics and Devices of the Ministry of Education Key Laboratory of Photonics Technology for Information School of Electronic and Information Engineering Xi' an Jiaotong University Xi 'an 710049 China

White organic light-emitting device (WOLEDs) employing molecular mixed host (MH) is demonstrated by *** spin-coated film functions as light-emitting layer and hole transporting layer,with the former formed by spin-coating solution containing MH of NPB (N.N'-Bis(naphthalene-1-yl)-N,N'-bis(phenyl)-benzidine) and MADN (2-methyl-9,10-di(2-naphthyl) anthracene),blue dye (4,4'-bis[2-(4-(N,N-diphenylamino)phenyl)vinyl]biphenyl) and yellow dye (5,6,11,12-tetraphenylnaphacene).The performances of the devices made with different mixed ratio of MH are *** is found that the device performances depends on the MH ratio,and under the optimal NPB:MADN ratio (60:40),the WOLEDs show a maximum luminance of 24 671 cd/m2 and a current efficiency of 5.8 cd/A for the practical luminance of 1000 cd/*** effect of MH ratio on device performances can be attributed to the difference of hole mobility between the NPB and MADN.

关键词：白色有机发光器件高分子材料制备设备旋涂混合比例装置性能空穴传输层 NPB

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